BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,851,044	A→G	T296A (ACG→GCG)	hypD →	HypD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,851,044	0	A	G	99.6%	714.3 / ‑4.9	224	T296A (ACG→GCG)	hypD	HypD
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (114/109); minor base T (1/0); total (115/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 233 total aligned reads displayed.

GCGCAACAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGACGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTGTGGTGAG  >  NC_000913/2850954‑2851133

gcgcAACAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCg                                                                                         <  2:842044/93‑1 (MQ=255)

  gcAACAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGcc                                                                                       >  2:1136645/1‑93 (MQ=255)

   cAACAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCg                                                                                      <  1:5642594/93‑1 (MQ=255)

    aaCAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCgg                                                                                     >  1:4572226/1‑93 (MQ=255)

    aaCAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCgg                                                                                     >  1:5980646/1‑93 (MQ=255)

     aCAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGa                                                                                    >  1:3453925/1‑93 (MQ=255)

     aCAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGa                                                                                    >  1:6390351/1‑93 (MQ=255)

     aCAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGa                                                                                    >  2:3366854/1‑93 (MQ=255)

       aGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGAtt                                                                                  >  2:2782333/1‑93 (MQ=255)

        ggCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTa                                                                                 <  1:5826627/93‑1 (MQ=255)

         gCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTAt                                                                                >  2:2607411/1‑93 (MQ=255)

         gCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTAt                                                                                >  2:4336533/1‑93 (MQ=255)

          cGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATc                                                                               <  2:3036886/93‑1 (MQ=255)

           gATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCa                                                                              <  2:2569044/93‑1 (MQ=255)

           gATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCa                                                                              >  2:2020284/1‑93 (MQ=255)

            aTTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCaa                                                                             >  2:232452/1‑93 (MQ=255)

            aTTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCaa                                                                             <  1:6432213/93‑1 (MQ=255)

            aTTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCaa                                                                             <  2:939437/93‑1 (MQ=255)

             ttGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAAc                                                                            >  2:4667343/1‑93 (MQ=255)

              tGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACg                                                                           >  1:3228300/1‑93 (MQ=255)

               gCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGa                                                                          >  1:6044165/1‑93 (MQ=255)

               gCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGa                                                                          <  1:2562725/93‑1 (MQ=255)

               gCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGa                                                                          >  1:1438160/1‑93 (MQ=255)

               gCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGa                                                                          <  2:5157707/93‑1 (MQ=255)

                ccGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGAt                                                                         <  1:2913287/93‑1 (MQ=255)

                 cGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGAtt                                                                        <  1:1605700/93‑1 (MQ=255)

                  gATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGAttg                                                                       >  2:486104/1‑92 (MQ=255)

                  gATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTc                                                                       <  2:604042/93‑1 (MQ=255)

                   aTGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCg                                                                      >  1:4533829/1‑93 (MQ=255)

                   aTGTGTTCTGTGTCAACGGAGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCg                                                                      >  1:6519865/1‑93 (MQ=255)

                    tgtgTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGGGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGa                                                                     >  2:3562474/1‑93 (MQ=255)

                    tgtgTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGa                                                                     <  2:1900921/93‑1 (MQ=255)

                    tgtgTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGa                                                                     <  1:2306097/93‑1 (MQ=255)

                     gtgtTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGAt                                                                    >  2:4081818/1‑93 (MQ=255)

                       gtTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGc                                                                  <  1:2991027/93‑1 (MQ=255)

                        ttCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGcc                                                                 >  1:3588925/1‑93 (MQ=255)

                          cTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGa                                                               >  2:5452415/1‑93 (MQ=255)

                          cTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGa                                                               <  1:3084871/93‑1 (MQ=255)

                           tgtgTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGaa                                                              <  1:6625705/93‑1 (MQ=255)

                            gtgtCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAg                                                             >  2:2155618/1‑93 (MQ=255)

                             tgtCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGc                                                            <  1:4648893/93‑1 (MQ=255)

                               tCAACGGCGACAGCGAATGGCGCGGCTTAGTCGTGTTTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGcac                                                          >  1:744980/1‑93 (MQ=255)

                               tCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGcac                                                          >  1:1955682/1‑93 (MQ=255)

                                 aaCGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACAt                                                        >  1:1241346/1‑93 (MQ=255)

                                 aaCGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACAt                                                        <  2:2362431/93‑1 (MQ=255)

                                 aaCGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACAt                                                        <  1:114633/93‑1 (MQ=255)

                                  aCGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACAtt                                                       >  1:4114288/1‑93 (MQ=255)

                                    ggCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTc                                                     <  2:4451477/93‑1 (MQ=255)

                                    ggCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTc                                                     <  1:3914192/93‑1 (MQ=255)

                                     gCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTcc                                                    <  1:4099863/93‑1 (MQ=255)

                                        aCAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGcc                                                 >  1:5481620/1‑93 (MQ=255)

                                         cAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGccc                                                <  1:6099753/93‑1 (MQ=255)

                                         cAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCCTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGccc                                                <  2:5868737/93‑1 (MQ=255)

                                            cGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGc                                             <  2:5636932/93‑1 (MQ=255)

                                                tGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCg                                         >  2:1781102/1‑93 (MQ=255)

                                                 ggCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCgc                                        >  2:2950681/1‑93 (MQ=255)

                                                   cgcgGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCgcag                                      <  2:1969296/93‑1 (MQ=255)

                                                     cgGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCgcagcg                                    >  1:1680278/1‑92 (MQ=255)

                                                     cgGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCgcagca                                    >  2:5007813/1‑93 (MQ=255)

                                                      ggCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGcagcag                                   <  2:171966/93‑1 (MQ=255)

                                                        cTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGt                                 <  1:993010/93‑1 (MQ=255)

                                                         ttAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAggcc                                >  2:3996401/1‑91 (MQ=255)

                                                         ttAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTc                                >  2:2411284/1‑93 (MQ=255)

                                                            ggCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGc                             >  1:1931476/1‑93 (MQ=255)

                                                            ggCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGc                             <  2:4108563/93‑1 (MQ=255)

                                                             gCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCg                            >  2:1397172/1‑93 (MQ=255)

                                                               gTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGAt                          >  2:225747/1‑93 (MQ=255)

                                                               gTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGAt                          >  1:2185418/1‑93 (MQ=255)

                                                                tGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATg                         >  1:5765051/1‑93 (MQ=255)

                                                                 gATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGa                        >  2:4988972/1‑93 (MQ=255)

                                                                 gATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGa                        >  2:5400611/1‑93 (MQ=255)

                                                                  aTTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAGCGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGAc                       <  2:6329579/93‑1 (MQ=255)

                                                                  aTTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGAc                       >  1:4786747/1‑93 (MQ=255)

                                                                   ttGAATCTTCTGGCGTGCACCTGTCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTACGCCCGGCACCGCAGCAGGTCTGCGATGAcc                      >  2:5755436/1‑93 (MQ=255)

                                                                   ttGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGAcc                      >  2:1261700/1‑93 (MQ=255)

                                                                   ttGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGAcc                      >  2:1449330/1‑93 (MQ=255)

                                                                   ttGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGAcc                      >  2:2432514/1‑93 (MQ=255)

                                                                    tGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGAccc                     >  1:3512168/1‑93 (MQ=255)

                                                                     gAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcg                    <  1:2028989/93‑1 (MQ=255)

                                                                      aaTCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgc                   <  1:1329849/93‑1 (MQ=255)

                                                                      aaTCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgc                   <  1:755785/93‑1 (MQ=255)

                                                                       aTCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgcg                  >  1:5039118/1‑93 (MQ=255)

                                                                       aTCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgcg                  >  1:2531233/1‑93 (MQ=255)

                                                                        tcttctGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgcgc                 >  1:2270877/1‑93 (MQ=255)

                                                                         cttctGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgcgcg                >  2:644605/1‑93 (MQ=255)

                                                                           tctGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgcgcgcg              >  2:6128569/1‑93 (MQ=255)

                                                                            ctGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgcgcgcgc             <  2:1642840/93‑1 (MQ=255)

                                                                             tGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgcgcgcgcg            >  1:1801799/1‑93 (MQ=255)

                                                                              tgCGTGCGCCTGGCGCGGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGt           <  1:5401632/92‑1 (MQ=255)

                                                                              ggCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGt           >  1:4820034/1‑93 (MQ=255)

                                                                              ggCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGt           <  2:3005840/93‑1 (MQ=255)

                                                                               gCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGtt          <  2:90277/93‑1 (MQ=255)

                                                                               gCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGtt          <  1:4071204/93‑1 (MQ=255)

                                                                                cGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTtg         <  1:2522052/93‑1 (MQ=255)

                                                                                 gTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTtgt        <  2:6541217/93‑1 (MQ=255)

                                                                                   gCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTgtgg      <  1:334908/93‑1 (MQ=255)

                                                                                     aCCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTgtggtg    <  2:3176065/93‑1 (MQ=255)

                                                                                      ccTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTgtggtgg   >  2:311963/1‑92 (MQ=255)

                                                                                       cTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTGTGGTGAg  >  2:5121807/1‑93 (MQ=255)

                                                                                       cTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTGTGGTGAg  >  1:5219035/1‑93 (MQ=255)

GCGCAACAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGACGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTGTGGTGAG  >  NC_000913/2850954‑2851133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`