BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,953,377	T→C	T217A (ACG→GCG)	recB ←	RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,953,377	0	T	C	99.6%	861.4 / NA	264	T217A (ACG→GCG)	recB	RecB
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (135/128); minor base A (1/0); total (136/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

TCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCC  >  NC_000913/2953287‑2953466

tCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGctt                                                                                         >  1:6357364/1‑93 (MQ=255)

tCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAAATTGCGCGTGACGGGAAGCCAGCGctt                                                                                         >  2:6732121/1‑93 (MQ=255)

 cAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtc                                                                                        >  1:3854197/1‑93 (MQ=255)

 cAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtc                                                                                        <  2:1243688/93‑1 (MQ=255)

  aGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtca                                                                                       >  2:3671546/1‑93 (MQ=255)

  aGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtca                                                                                       <  1:2807180/93‑1 (MQ=255)

   gcgcATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtcat                                                                                      <  2:5448748/93‑1 (MQ=255)

   gcgcATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtcat                                                                                      >  1:6230012/1‑93 (MQ=255)

   gcgcATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtcat                                                                                      >  1:4151805/1‑93 (MQ=255)

      cATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTcatcat                                                                                   >  1:2129882/1‑93 (MQ=255)

      cATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTcatcat                                                                                   >  1:80823/1‑93 (MQ=255)

      cATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTcatcat                                                                                   >  1:443356/1‑93 (MQ=255)

        tCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCg                                                                                 <  2:1482178/93‑1 (MQ=255)

         ccAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCgg                                                                                <  2:1347443/93‑1 (MQ=255)

         ccAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCgg                                                                                >  2:5795949/1‑93 (MQ=255)

          cAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCggg                                                                               >  2:3827921/1‑93 (MQ=255)

           aGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggc                                                                              >  2:2181680/1‑93 (MQ=255)

            gTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggcg                                                                             >  1:894260/1‑93 (MQ=255)

            gTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggcg                                                                             >  2:2643653/1‑93 (MQ=255)

             ttCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggcgg                                                                            <  1:3087147/93‑1 (MQ=255)

             ttCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggcgg                                                                            >  2:4908061/1‑93 (MQ=255)

             ttCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggcgg                                                                            >  2:5638899/1‑93 (MQ=255)

               cACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGgcggcg                                                                          >  2:2230039/1‑93 (MQ=255)

               cACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGgcggcg                                                                          >  2:3938519/1‑93 (MQ=255)

               cACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTCGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGgcggcg                                                                          <  2:1968946/93‑1 (MQ=255)

                aCCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGcggcgg                                                                         >  1:5863875/1‑93 (MQ=255)

                aCCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGcggcgg                                                                         >  1:5283891/1‑93 (MQ=255)

                 cccACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGcggcggg                                                                        >  1:3401302/1‑92 (MQ=255)

                 cccACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGt                                                                        >  1:2397933/1‑93 (MQ=255)

                   cACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGATTCATCATCGGGCGGCGGTGc                                                                      >  1:1923974/1‑93 (MQ=255)

                   cACTGCGTCGCGACACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGc                                                                      >  2:541192/1‑93 (MQ=255)

                    aCTGNGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCt                                                                     <  2:3053016/93‑1 (MQ=255)

                    aCTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCt                                                                     <  2:4113757/93‑1 (MQ=255)

                    aCTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCt                                                                     >  1:4972978/1‑93 (MQ=255)

                     cTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCtt                                                                    <  2:5031309/93‑1 (MQ=255)

                       gCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTg                                                                  >  2:2271427/1‑93 (MQ=255)

                        cGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGa                                                                 <  2:3737949/93‑1 (MQ=255)

                         gTCGCCCCCCTGCTTGTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGAt                                                                <  1:2594905/93‑1 (MQ=255)

                          tCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATa                                                               >  2:2366649/1‑93 (MQ=255)

                             cgcCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAAcc                                                            <  1:5133927/93‑1 (MQ=255)

                              gcCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCg                                                           <  2:6288721/93‑1 (MQ=255)

                                cACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGgc                                                         >  1:5644140/1‑93 (MQ=255)

                                  ctgctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGgcgc                                                       >  2:5209959/1‑93 (MQ=255)

                                   tgctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCt                                                      >  2:1397793/1‑93 (MQ=255)

                                   tgctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCt                                                      >  2:4695624/1‑93 (MQ=255)

                                    tctgTTTTACCGTATCAATACGCGCCACAATCTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCtt                                                     <  1:546068/92‑1 (MQ=255)

                                    gctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCtt                                                     >  1:6673302/1‑93 (MQ=255)

                                     ctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTc                                                    >  1:290214/1‑93 (MQ=255)

                                          ttcccGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCtt                                               <  1:4749832/90‑1 (MQ=255)

                                          ttACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCtt                                               <  1:272488/93‑1 (MQ=255)

                                             ccGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCa                                            <  2:2804820/93‑1 (MQ=255)

                                             ccGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCa                                            <  1:2629267/93‑1 (MQ=255)

                                             ccGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCa                                            >  1:4670267/1‑93 (MQ=255)

                                                tATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAt                                         <  2:5862252/93‑1 (MQ=255)

                                                tATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAt                                         >  2:1128132/1‑93 (MQ=255)

                                                tATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAt                                         <  2:6404642/93‑1 (MQ=255)

                                                 aTCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATa                                        >  2:4199579/1‑93 (MQ=255)

                                                 aTCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATa                                        >  2:2738711/1‑93 (MQ=255)

                                                  tCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATaa                                       >  2:2881962/1‑93 (MQ=255)

                                                   cAATACGCGCCACAATTTGCGCGTGACGGGAAGCGCGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGACTTGCAGATAAc                                      >  2:5252503/1‑93 (MQ=255)

                                                   cAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAAc                                      <  1:1078744/93‑1 (MQ=255)

                                                    aaTACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACg                                     >  1:4328616/1‑93 (MQ=255)

                                                     aTACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGa                                    >  2:781995/1‑93 (MQ=255)

                                                     aTACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGa                                    >  1:1739541/1‑93 (MQ=255)

                                                      tACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAt                                   >  1:6618007/1‑93 (MQ=255)

                                                      tACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAt                                   >  2:3273705/1‑93 (MQ=255)

                                                      tACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAt                                   <  1:3721005/93‑1 (MQ=255)

                                                      tACGCGCCACAATTTGCACGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAt                                   <  2:5063792/93‑1 (MQ=255)

                                                        cgcgCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTa                                 <  1:1861659/93‑1 (MQ=255)

                                                          cgcCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAat                               <  1:1180533/93‑1 (MQ=255)

                                                           gcCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAata                              <  2:6122984/93‑1 (MQ=255)

                                                           gcCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAata                              <  2:4602179/93‑1 (MQ=255)

                                                             cacaATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATc                            >  2:873465/1‑93 (MQ=255)

                                                             cacaATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATc                            <  1:5405230/93‑1 (MQ=255)

                                                               caATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATcgc                          >  2:1843150/1‑93 (MQ=255)

                                                                aaTTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATcgcg                         >  1:3771702/1‑93 (MQ=255)

                                                                 aTTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCgcgc                        <  2:3224077/93‑1 (MQ=255)

                                                                  tttGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgca                       >  1:4597352/1‑93 (MQ=255)

                                                                   ttGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgcag                      <  1:1534610/93‑1 (MQ=255)

                                                                     gcgcGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgcagca                    <  2:6023420/93‑1 (MQ=255)

                                                                      cgcgTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCaa                   >  1:543613/1‑93 (MQ=255)

                                                                       gcgTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAAc                  >  2:5539598/1‑93 (MQ=255)

                                                                       gcgTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGGGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAAc                  >  1:3988697/1‑93 (MQ=255)

                                                                         gttaCGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGc                <  2:5913904/90‑1 (MQ=255)

                                                                          tGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGcc               <  1:2004849/93‑1 (MQ=255)

                                                                           gACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCt              >  2:2511250/1‑93 (MQ=255)

                                                                             cGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGc            >  1:2962286/1‑93 (MQ=255)

                                                                               ggAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCgg          >  1:575146/1‑93 (MQ=255)

                                                                               ggAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCgg          >  2:6398961/1‑93 (MQ=255)

                                                                               ggAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCgg          >  1:942627/1‑93 (MQ=255)

                                                                                  aGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGccc       >  2:5478236/1‑93 (MQ=255)

                                                                                  aGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGccc       <  1:5036993/93‑1 (MQ=255)

                                                                                  aGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGccc       >  2:448127/1‑93 (MQ=255)

                                                                                  aGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGccc       >  2:4557671/1‑93 (MQ=255)

                                                                                  aGCCAGCGCTTCATAATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGccc       >  2:3496504/1‑93 (MQ=255)

                                                                                   gCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCt      <  1:1425923/93‑1 (MQ=255)

                                                                                   gCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCt      <  1:1014254/93‑1 (MQ=255)

                                                                                     cAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCttt    >  1:6153949/1‑93 (MQ=255)

                                                                                     cAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCCGCGCTTCGCCTTGCAGATAACGATTAATATCGGGCAGCAACGCCTGCGGCCCttt    >  1:2164636/1‑93 (MQ=255)

                                                                                       gcgcTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTcc  <  2:4800322/93‑1 (MQ=255)

TCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCC  >  NC_000913/2953287‑2953466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`