BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,854	T→C	L271P (CTG→CCG)	lysR →	LysR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,854	0	T	C	99.5%	669.6 / NA	211	L271P (CTG→CCG)	lysR	LysR
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (100/110); total (100/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

CGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTC  >  NC_000913/2977764‑2977943

cGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGa                                                                                         >  1:2965630/1‑93 (MQ=255)

    ggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCg                                                                                     <  1:4977209/93‑1 (MQ=255)

      ttAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGGTCCGTTCACCGTCAGCCCGCTCCGcc                                                                                   >  1:5810854/1‑93 (MQ=255)

        aaCCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGcccc                                                                                 >  2:243904/1‑93 (MQ=255)

         aCCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGccccc                                                                                <  2:5144413/93‑1 (MQ=255)

          cccGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCt                                                                               <  2:776746/93‑1 (MQ=255)

            cGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGc                                                                             >  2:173951/1‑93 (MQ=255)

             gCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCa                                                                            <  2:6684811/93‑1 (MQ=255)

             gCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCa                                                                            >  2:3359940/1‑93 (MQ=255)

              cTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCAc                                                                           <  2:2530712/93‑1 (MQ=255)

                cACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCg                                                                         >  1:3862305/1‑93 (MQ=255)

                cACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCg                                                                         >  2:1988698/1‑93 (MQ=255)

                 aCCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGc                                                                        >  1:1344677/1‑93 (MQ=255)

                  ccGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGcc                                                                       <  2:5201669/93‑1 (MQ=255)

                  ccGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGcc                                                                       <  2:2333015/93‑1 (MQ=255)

                   cGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGccc                                                                      <  2:896250/93‑1 (MQ=255)

                   cGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGccc                                                                      <  1:1168784/93‑1 (MQ=255)

                    gCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCg                                                                     >  1:5305692/1‑93 (MQ=255)

                     cACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGt                                                                    >  2:659365/1‑93 (MQ=255)

                      aCTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtc                                                                   >  2:4013869/1‑93 (MQ=255)

                      aCTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtc                                                                   >  2:4369304/1‑93 (MQ=255)

                      aCTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtc                                                                   >  1:2203503/1‑93 (MQ=255)

                      aCTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtc                                                                   >  1:5037416/1‑93 (MQ=255)

                       cTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtca                                                                  <  2:5266913/93‑1 (MQ=255)

                       cTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtca                                                                  >  2:1014935/1‑93 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcat                                                                 >  1:358252/1‑93 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcat                                                                 >  1:5409152/1‑93 (MQ=255)

                          gATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcatca                                                               <  1:3940175/93‑1 (MQ=255)

                           aTTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAg                                                              <  1:2445180/93‑1 (MQ=255)

                            ttATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgc                                                             >  2:666699/1‑93 (MQ=255)

                            ttATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgc                                                             >  1:5704024/1‑93 (MQ=255)

                             tATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgcg                                                            >  2:2386551/1‑93 (MQ=255)

                              aTGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgcgc                                                           >  1:6541011/1‑93 (MQ=255)

                               tGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCt                                                          <  1:3112253/93‑1 (MQ=255)

                               tGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCt                                                          >  2:3605955/1‑93 (MQ=255)

                                 cGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTgg                                                        <  1:2337163/93‑1 (MQ=255)

                                 cGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTgg                                                        <  1:5660003/93‑1 (MQ=255)

                                  ggCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGt                                                       <  1:1078462/93‑1 (MQ=255)

                                  ggCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGt                                                       <  2:4575156/93‑1 (MQ=255)

                                     aaGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCa                                                    >  1:3797550/1‑93 (MQ=255)

                                      aGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAg                                                   >  2:2038530/1‑93 (MQ=255)

                                      aGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAg                                                   >  2:6234760/1‑93 (MQ=255)

                                       gCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAgg                                                  <  1:6157282/93‑1 (MQ=255)

                                        cGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGc                                                 <  1:1692254/93‑1 (MQ=255)

                                        cGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGc                                                 <  1:3358652/93‑1 (MQ=255)

                                         ggTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCg                                                <  2:4244363/93‑1 (MQ=255)

                                          gTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGt                                               >  2:5310120/1‑93 (MQ=255)

                                          gTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGt                                               <  2:182763/93‑1 (MQ=255)

                                           tttAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGtt                                              <  1:2488120/93‑1 (MQ=255)

                                           tttAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGtt                                              >  2:1087200/1‑93 (MQ=255)

                                             tAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTa                                            <  2:5767789/93‑1 (MQ=255)

                                              aGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAg                                           <  2:3083039/93‑1 (MQ=255)

                                               gtggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGt                                          <  2:5720682/93‑1 (MQ=255)

                                                 ggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTgg                                        <  1:650699/93‑1 (MQ=255)

                                                     cggcggTGCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCAt                                    <  1:4613793/93‑1 (MQ=255)

                                                      ggcggTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCAtt                                   <  1:6724407/93‑1 (MQ=255)

                                                          gTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACa                               <  1:4846855/93‑1 (MQ=255)

                                                          gTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGGTTAGTGGGCATTTACa                               >  1:5867874/1‑93 (MQ=255)

                                                           ttCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACaa                              <  2:4888105/93‑1 (MQ=255)

                                                           ttCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACaa                              <  1:6682560/93‑1 (MQ=255)

                                                            tCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAg                             <  2:165254/93‑1 (MQ=255)

                                                              aGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCg                           <  2:1300430/93‑1 (MQ=255)

                                                              aGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCg                           >  1:2811175/1‑93 (MQ=255)

                                                               gTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgg                          >  2:448686/1‑93 (MQ=255)

                                                               gTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgg                          >  1:4752717/1‑93 (MQ=255)

                                                               gTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgg                          >  1:1417306/1‑93 (MQ=255)

                                                               gTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgg                          <  1:1957500/93‑1 (MQ=255)

                                                               gTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgg                          >  1:2730783/1‑93 (MQ=255)

                                                                tATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCggg                         <  1:2610062/93‑1 (MQ=255)

                                                                tATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCggg                         <  2:6638917/93‑1 (MQ=255)

                                                                  ttGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGt                       <  1:1003698/93‑1 (MQ=255)

                                                                  ttGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGt                       >  1:5149503/1‑93 (MQ=255)

                                                                  ttGCGGTTCCGTCCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGt                       <  2:3443240/93‑1 (MQ=255)

                                                                    gCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTa                     >  2:4151366/1‑93 (MQ=255)

                                                                     cGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTAc                    >  2:267552/1‑93 (MQ=255)

                                                                     cGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTAc                    <  1:4571326/93‑1 (MQ=255)

                                                                     cGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTAc                    >  1:3652406/1‑93 (MQ=255)

                                                                        ttCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGa                 >  1:4327586/1‑93 (MQ=255)

                                                                          ccGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGaaa               <  1:4188334/93‑1 (MQ=255)

                                                                          ccGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGaaa               <  2:4376626/93‑1 (MQ=255)

                                                                          ccGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGaaa               >  2:1483953/1‑93 (MQ=255)

                                                                           cGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAAc              >  2:2722889/1‑93 (MQ=255)

                                                                           cGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAAc              <  1:3015714/93‑1 (MQ=255)

                                                                            gTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCTGGGCTACCGAAACt             >  2:2919866/1‑93 (MQ=255)

                                                                            gTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACt             >  2:2313869/1‑93 (MQ=255)

                                                                            gTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACt             <  2:5941618/93‑1 (MQ=255)

                                                                            gTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACt             <  2:1921248/93‑1 (MQ=255)

                                                                             ttCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTATTGGGCATTTACAAGCGGGGGTACCGAAACTg            >  1:5969709/1‑93 (MQ=255)

                                                                             ttCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTg            >  1:866738/1‑93 (MQ=255)

                                                                              tCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTgg           >  1:6307024/1‑93 (MQ=255)

                                                                               cACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGt          <  2:3825161/93‑1 (MQ=255)

                                                                               cACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGt          <  1:6047961/93‑1 (MQ=255)

                                                                               cACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGt          <  1:5534049/93‑1 (MQ=255)

                                                                                 ccGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCa        >  1:3026366/1‑93 (MQ=255)

                                                                                 ccGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCa        <  2:4517366/93‑1 (MQ=255)

                                                                                    tCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACtt     <  1:2537496/93‑1 (MQ=255)

                                                                                      aGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTtct   >  2:2119364/1‑93 (MQ=255)

                                                                                       gCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTtctc  >  2:2629917/1‑93 (MQ=255)

                                                                                       gCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTtctc  <  2:472139/93‑1 (MQ=255)

                                                                                       gCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTtctc  <  2:2965630/93‑1 (MQ=255)

CGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTC  >  NC_000913/2977764‑2977943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`