BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,905	A→G	S584P (TCG→CCG)	tktA ←	transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,905	0	A	G	99.6%	792.7 / NA	245	S584P (TCG→CCG)	tktA	transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (121/123); minor base C (0/1); total (121/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

CCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGACATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGA  >  NC_000913/3077815‑3077994

ccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCa                                                                                         >  1:4107018/1‑93 (MQ=255)

ccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCa                                                                                         <  2:1794296/93‑1 (MQ=255)

ccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCa                                                                                         <  2:3129736/93‑1 (MQ=255)

 atcTTCTACAGCATCTATTGCAGTAACCTCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCAt                                                                                        <  1:3452936/91‑1 (MQ=255)

  gCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATg                                                                                       >  2:857170/1‑93 (MQ=255)

  gCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATg                                                                                       <  2:4918737/93‑1 (MQ=255)

     tCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGac                                                                                    <  1:5583884/93‑1 (MQ=255)

          aGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcaccac                                                                               >  2:1672074/1‑93 (MQ=255)

          aGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcaccac                                                                               <  2:1524270/93‑1 (MQ=255)

           gCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcg                                                                              >  2:5960462/1‑93 (MQ=255)

              aCGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgcgc                                                                           >  2:4035182/1‑93 (MQ=255)

                 cgTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCt                                                                        <  1:5732246/93‑1 (MQ=255)

                  gTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCtt                                                                       <  2:3504885/93‑1 (MQ=255)

                  gTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCtt                                                                       <  1:4258947/93‑1 (MQ=255)

                   tGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCttt                                                                      <  1:1869913/93‑1 (MQ=255)

                   tGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCttt                                                                      <  1:4018126/93‑1 (MQ=255)

                    gCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTc                                                                     >  1:1693809/1‑93 (MQ=255)

                    gCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTc                                                                     >  2:731772/1‑93 (MQ=255)

                    gCAGTAACCGCTTTCGGCAGTACGGATTCACGATAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTc                                                                     <  2:2396428/93‑1 (MQ=255)

                      aGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCAc                                                                   <  1:3421670/93‑1 (MQ=255)

                       gTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCAcc                                                                  >  2:2485389/1‑92 (MQ=255)

                       gTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACg                                                                  >  2:1210797/1‑93 (MQ=255)

                       gTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACg                                                                  >  1:4548983/1‑93 (MQ=255)

                         aaCCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGTTAGACGGCATGGACACCACGCGCGCTTTCACGcc                                                                <  1:3172916/93‑1 (MQ=255)

                          aCCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCt                                                               >  1:3929479/1‑93 (MQ=255)

                          aCCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCt                                                               <  1:1901914/93‑1 (MQ=255)

                          aCCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCt                                                               <  2:1320411/93‑1 (MQ=255)

                          aCCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCt                                                               <  2:4010246/93‑1 (MQ=255)

                          aCCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCt                                                               <  2:4983849/93‑1 (MQ=255)

                           ccGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCtt                                                              <  2:3630189/93‑1 (MQ=255)

                            cGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTc                                                             <  2:4755305/93‑1 (MQ=255)

                            cGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTc                                                             >  1:6137103/1‑93 (MQ=255)

                            cGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTc                                                             <  1:6036648/93‑1 (MQ=255)

                            cGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTc                                                             <  2:1344862/93‑1 (MQ=255)

                             gCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCg                                                            >  2:4597914/1‑93 (MQ=255)

                                ttCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca                                                         >  2:5167382/1‑93 (MQ=255)

                                     cAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcag                                                    <  1:4578709/93‑1 (MQ=255)

                                       gTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGtt                                                  >  1:4184538/1‑93 (MQ=255)

                                       gTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGtt                                                  <  1:5915019/93‑1 (MQ=255)

                                       gTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGtt                                                  <  2:4635605/93‑1 (MQ=255)

                                       gTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGtt                                                  <  2:2410805/93‑1 (MQ=255)

                                       gTACGGATGCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGtt                                                  <  1:3247741/93‑1 (MQ=255)

                                         aCGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGtttt                                                <  2:4139187/93‑1 (MQ=255)

                                          cGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGGATGGACACCACGCGCGCGTTCACGCCTTCGGCAGTCAGttttt                                               <  2:6005427/93‑1 (MQ=255)

                                          cGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGttttt                                               <  1:2234701/93‑1 (MQ=255)

                                          cGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGttttt                                               >  2:6196840/1‑93 (MQ=255)

                                           ggATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTc                                              <  1:4300327/93‑1 (MQ=255)

                                            gATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCg                                             >  1:6307418/1‑93 (MQ=255)

                                            gATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCg                                             <  2:2058792/93‑1 (MQ=255)

                                             aTTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGt                                            >  2:4817576/1‑93 (MQ=255)

                                             aTTCACGCTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGCCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGt                                            <  2:1608225/93‑1 (MQ=255)

                                              ttCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTa                                           >  2:5614635/1‑93 (MQ=255)

                                              ttCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCCTTCACGCCTTCGGCAGTCAGTTTTTCGTa                                           >  1:2838000/1‑93 (MQ=255)

                                               tCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAg                                          <  1:1475576/93‑1 (MQ=255)

                                                cACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAgg                                         <  2:2706535/93‑1 (MQ=255)

                                                 aCGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgc                                        >  2:2757628/1‑93 (MQ=255)

                                                  cGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgca                                       <  1:3016750/93‑1 (MQ=255)

                                                    gTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgcagc                                     <  1:1275547/93‑1 (MQ=255)

                                                       agcagcATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAAc                                  >  2:1433325/1‑93 (MQ=255)

                                                        gcagcaTCCTTCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACa                                 <  2:2848352/93‑1 (MQ=255)

                                                          agcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGc                               <  1:5316928/93‑1 (MQ=255)

                                                           gcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCGTGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCCGTTTTTCGTAGGCAGCAAGAGcc                              >  1:2706172/1‑93 (MQ=255)

                                                           gcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGcc                              >  2:2580206/1‑93 (MQ=255)

                                                           gcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGcc                              >  2:5417731/1‑93 (MQ=255)

                                                            caTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCa                             <  2:5046406/93‑1 (MQ=255)

                                                             aTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAg                            <  1:6501284/93‑1 (MQ=255)

                                                              tCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGt                           >  1:3136778/1‑93 (MQ=255)

                                                              tCCTGCTTGTCAAATGCGACGGTAGAAGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGCTTTTCGTAGGCAGACACAGCCAGt                           >  1:863963/1‑93 (MQ=255)

                                                                cTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTc                         <  1:3591589/93‑1 (MQ=255)

                                                                cTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTc                         >  1:2394647/1‑93 (MQ=255)

                                                                cTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTc                         <  1:817205/93‑1 (MQ=255)

                                                                cTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTc                         <  2:220870/93‑1 (MQ=255)

                                                                 tGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCa                        >  1:2637421/1‑93 (MQ=255)

                                                                 cgCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCa                        <  1:1997918/92‑1 (MQ=255)

                                                                  gCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCaa                       <  2:1063149/93‑1 (MQ=255)

                                                                    ttGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACt                     <  1:5465045/93‑1 (MQ=255)

                                                                    ttGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACt                     <  1:538338/93‑1 (MQ=255)

                                                                     tGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACtt                    >  1:1511190/1‑93 (MQ=255)

                                                                      gTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTc                   <  1:1741555/93‑1 (MQ=255)

                                                                      gTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTc                   >  1:3059636/1‑93 (MQ=255)

                                                                      gTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTc                   <  1:4955371/93‑1 (MQ=255)

                                                                       tCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCt                  <  1:3804099/93‑1 (MQ=255)

                                                                       tCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCt                  <  2:6446455/93‑1 (MQ=255)

                                                                         aaaTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGa                >  2:3375066/1‑93 (MQ=255)

                                                                         aaaTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGa                >  1:1618979/1‑93 (MQ=255)

                                                                         aaaTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGACAGTTCAACTTCTGa                >  1:5243088/1‑93 (MQ=255)

                                                                          aaTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGaa               <  2:3746598/93‑1 (MQ=255)

                                                                          aaTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGaa               <  2:2919346/93‑1 (MQ=255)

                                                                          aaTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGaa               >  1:813777/1‑93 (MQ=255)

                                                                            tGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAAcc             <  2:5471806/93‑1 (MQ=255)

                                                                             gCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCg            >  2:2600465/1‑93 (MQ=255)

                                                                                tCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTa         <  1:5825332/93‑1 (MQ=255)

                                                                                tCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTa         >  2:660207/1‑93 (MQ=255)

                                                                                  ggTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGc       <  1:4729698/93‑1 (MQ=255)

                                                                                  ggTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGc       >  1:5039285/1‑93 (MQ=255)

                                                                                    tAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGa     <  1:1151502/93‑1 (MQ=255)

                                                                                    tAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGa     <  2:1979252/93‑1 (MQ=255)

                                                                                    tAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGa     <  2:5817318/93‑1 (MQ=255)

                                                                                    tAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGa     <  1:6420670/93‑1 (MQ=255)

                                                                                     aGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGAt    >  2:6024967/1‑93 (MQ=255)

CCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGACATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGA  >  NC_000913/3077815‑3077994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`