BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	361,946	A→G	L153P (CTG→CCG)	lacY ←	galactoside permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	361,946	0	A	G	100.0%	682.1 / NA	213	L153P (CTG→CCG)	lacY	galactoside permease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (115/98); total (115/98)

Only 100 of 223 total aligned reads displayed.

AAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCT  >  NC_000913/361856‑362035

aaaaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGgc                                                                                         >  2:4207093/1‑93 (MQ=255)

aaaaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGgc                                                                                         <  2:2483801/93‑1 (MQ=255)

 aaaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGgcg                                                                                        >  1:1633395/1‑93 (MQ=255)

  aaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGgcgc                                                                                       >  2:795226/1‑93 (MQ=255)

   aCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGcc                                                                                      >  2:39345/1‑93 (MQ=255)

   aCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGcc                                                                                      >  2:548634/1‑93 (MQ=255)

    cGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGccc                                                                                     >  1:3304415/1‑93 (MQ=255)

    cGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGccc                                                                                     <  1:5363298/93‑1 (MQ=255)

      gCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAg                                                                                   >  1:1002435/1‑93 (MQ=255)

       cGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGc                                                                                  <  2:1748913/93‑1 (MQ=255)

       cGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGc                                                                                  <  1:339713/93‑1 (MQ=255)

        gAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAGCATGATGCCGACAATCGAGGCACACGGCGCCCAGcc                                                                                 >  2:3074659/1‑93 (MQ=255)

           gATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAac                                                                              >  2:6348030/1‑93 (MQ=255)

           gATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAac                                                                              <  2:1030638/93‑1 (MQ=255)

           gATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAac                                                                              <  1:6474641/93‑1 (MQ=255)

           gATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAac                                                                              <  2:4201278/93‑1 (MQ=255)

           gATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAac                                                                              >  1:343304/1‑93 (MQ=255)

           gATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAac                                                                              <  1:3574775/93‑1 (MQ=255)

             tGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAacac                                                                            >  2:3450223/1‑93 (MQ=255)

              gAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAAcaca                                                                           >  1:2698664/1‑93 (MQ=255)

               aGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAg                                                                          >  1:3944584/1‑93 (MQ=255)

                 tGCACAGCCAGAGACCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGcc                                                                        <  2:6429074/93‑1 (MQ=255)

                     caGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAAc                                                                    >  2:892735/1‑93 (MQ=255)

                      aGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACa                                                                   <  2:3391919/93‑1 (MQ=255)

                      aGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACa                                                                   <  1:3755920/93‑1 (MQ=255)

                      aGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACa                                                                   <  1:179461/93‑1 (MQ=255)

                      aGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACa                                                                   >  2:5087391/1‑93 (MQ=255)

                      aGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACa                                                                   <  2:4125841/93‑1 (MQ=255)

                          agagCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcg                                                               >  2:1895913/1‑93 (MQ=255)

                          agagCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACAGGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcg                                                               <  1:6716013/93‑1 (MQ=255)

                            agCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcg                                                             <  2:3555490/93‑1 (MQ=255)

                             gCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgc                                                            <  2:371839/93‑1 (MQ=255)

                             gCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgc                                                            >  2:4914078/1‑93 (MQ=255)

                              cccAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgcg                                                           <  2:3738935/93‑1 (MQ=255)

                              cccAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgcg                                                           >  1:2778730/1‑93 (MQ=255)

                              cccAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgcg                                                           >  1:4868230/1‑93 (MQ=255)

                                cagccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgcgcg                                                         >  1:3902900/1‑93 (MQ=255)

                                   ccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGAca                                                      >  2:3656008/1‑92 (MQ=255)

                                   ccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGAca                                                      >  1:5884238/1‑92 (MQ=255)

                                    cagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCa                                                     >  2:2466097/1‑93 (MQ=255)

                                      gAAAACAAACTGATTATTGATGGTGATCATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaaa                                                   <  1:2541360/93‑1 (MQ=255)

                                      gAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGGCCAGCCAACACAGCCAAACATCCGCGCGCGACCaaa                                                   >  2:4402576/1‑93 (MQ=255)

                                        aaacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAtt                                                 >  2:4072327/1‑93 (MQ=255)

                                        aaacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAtt                                                 >  1:3794058/1‑93 (MQ=255)

                                         aacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTc                                                >  1:1050179/1‑93 (MQ=255)

                                         aacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTc                                                <  2:1338173/93‑1 (MQ=255)

                                         aacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTc                                                >  2:6542064/1‑93 (MQ=255)

                                         aacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTc                                                <  1:4123319/93‑1 (MQ=255)

                                            aaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGaa                                             >  1:2418116/1‑93 (MQ=255)

                                             aacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGaaa                                            <  1:4709802/93‑1 (MQ=255)

                                              acTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAAt                                           >  1:5270422/1‑93 (MQ=255)

                                                 gATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTAc                                        >  1:3217996/1‑93 (MQ=255)

                                                 gATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTAc                                        >  1:4383406/1‑93 (MQ=255)

                                                  attattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACt                                       <  2:936444/93‑1 (MQ=255)

                                                  attattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACt                                       >  1:2744596/1‑93 (MQ=255)

                                                   ttattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTg                                      >  1:2588982/1‑93 (MQ=255)

                                                      ttGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGa                                   <  1:2915222/93‑1 (MQ=255)

                                                      ttGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGa                                   >  1:5143415/1‑93 (MQ=255)

                                                       tGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGAc                                  <  2:2352963/93‑1 (MQ=255)

                                                        gATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACg                                 >  1:4209341/1‑93 (MQ=255)

                                                        gATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACg                                 >  2:721713/1‑93 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCTAGCCAACACAGCCAAACATCCGCGCGTGACCAGATTCGAAATTACTGCGACGGc                               >  2:2051369/1‑93 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGc                               <  1:1297302/93‑1 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGc                               <  1:5600726/93‑1 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGc                               >  2:4829614/1‑93 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGc                               >  1:472557/1‑93 (MQ=255)

                                                           ggTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCt                              >  2:5856258/1‑93 (MQ=255)

                                                             tGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAANTCGAAATTACTGCGANGGCNGa                            >  2:6460939/1‑93 (MQ=255)

                                                               aaCATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACt                          >  2:6499157/1‑93 (MQ=255)

                                                                aCATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACtt                         <  1:1988755/93‑1 (MQ=255)

                                                                aCATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACCGCGACGGCTGACtt                         <  2:1452976/93‑1 (MQ=255)

                                                                  atgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtc                       >  1:4742953/1‑93 (MQ=255)

                                                                   tgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                      >  2:4608226/1‑93 (MQ=255)

                                                                   tgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                      <  1:5964011/93‑1 (MQ=255)

                                                                    gatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtctc                     <  2:2237357/93‑1 (MQ=255)

                                                                     atgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCa                    >  2:4859689/1‑93 (MQ=255)

                                                                     atgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCa                    >  1:2618018/1‑93 (MQ=255)

                                                                      tgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCaa                   <  1:898817/93‑1 (MQ=255)

                                                                        ccGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATa                 <  2:6190575/93‑1 (MQ=255)

                                                                         cGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATaa                <  2:5178741/93‑1 (MQ=255)

                                                                          gACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATaaa               >  2:5736349/1‑93 (MQ=255)

                                                                           aCAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAAt              >  2:2926309/1‑93 (MQ=255)

                                                                           aCAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCAGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAAt              >  2:4706011/1‑93 (MQ=255)

                                                                              aTCGAGGCACGCGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGcc           <  2:1113070/93‑1 (MQ=255)

                                                                              aTCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGcc           <  2:288546/93‑1 (MQ=255)

                                                                              aTCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGcc           >  1:2233181/1‑93 (MQ=255)

                                                                              aTCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGcc           >  1:4348839/1‑93 (MQ=255)

                                                                               tCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCct          >  2:331301/1‑93 (MQ=255)

                                                                                cGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCctc         <  2:321135/93‑1 (MQ=255)

                                                                                 gAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCctct        <  1:5948644/93‑1 (MQ=255)

                                                                                 gAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCctct        >  1:2075195/1‑93 (MQ=255)

                                                                                 gAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCctct        <  1:4566433/93‑1 (MQ=255)

                                                                                 gAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCctct        >  2:2689893/1‑93 (MQ=255)

                                                                                  aGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTa       >  2:6077463/1‑93 (MQ=255)

                                                                                   ggCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAc      <  2:1846672/93‑1 (MQ=255)

                                                                                   gccacacgGCGCCCAGCCAACACAGACAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAc      <  1:1501093/91‑1 (MQ=255)

                                                                                    gCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAct     <  1:5101180/93‑1 (MQ=255)

                                                                                    gCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAct     >  1:6165494/1‑93 (MQ=255)

                                                                                      acacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgc   >  1:303042/1‑93 (MQ=255)

                                                                                       cacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgct  >  2:1394612/1‑93 (MQ=255)

AAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCT  >  NC_000913/361856‑362035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`