BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,185,807	T→C	F131S (TTT→TCT)	yqiG →	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,185,807	0	T	C	100.0%	1044.3 / NA	312	F131S (TTT→TCT)	yqiG	putative membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (167/145); total (167/145)

Only 100 of 314 total aligned reads displayed.

GCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCTTGACT  >  NC_000913/3185718‑3185896

    aaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTAGCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCt                                                                                    <  2:335206/93‑1 (MQ=255)

     aCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTc                                                                                   <  2:149799/93‑1 (MQ=255)

       cTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAg                                                                                 <  1:1492146/93‑1 (MQ=255)

       cTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAg                                                                                 <  2:6006425/93‑1 (MQ=255)

       cTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAg                                                                                 >  1:2488838/1‑93 (MQ=255)

        tGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAgg                                                                                >  2:878065/1‑93 (MQ=255)

        tGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAgg                                                                                >  2:2624749/1‑93 (MQ=255)

        tGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAgg                                                                                >  1:4057672/1‑93 (MQ=255)

         gATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGc                                                                               >  2:4377581/1‑93 (MQ=255)

           tCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCAt                                                                             <  1:5824244/93‑1 (MQ=255)

            cGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATg                                                                            <  2:4990157/93‑1 (MQ=255)

            cGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATg                                                                            <  2:3072474/93‑1 (MQ=255)

             gATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATgg                                                                           <  2:2409513/93‑1 (MQ=255)

               tGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGtt                                                                         >  1:3879366/1‑93 (MQ=255)

                ggTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCACGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTa                                                                        <  1:6625858/93‑1 (MQ=255)

                 gttgttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaa                                                                       <  1:5373573/93‑1 (MQ=255)

                    gttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAAt                                                                    <  2:6440960/93‑1 (MQ=255)

                     ttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATa                                                                   <  1:826518/93‑1 (MQ=255)

                     ttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATa                                                                   <  1:4680970/93‑1 (MQ=255)

                      tACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATAc                                                                  >  1:2974390/1‑93 (MQ=255)

                      tACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATAc                                                                  <  2:725456/93‑1 (MQ=255)

                       aCCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACa                                                                 >  1:4067530/1‑93 (MQ=255)

                          cTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAg                                                              >  1:3936670/1‑93 (MQ=255)

                           tATCGAAACAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGa                                                             >  1:6554179/1‑93 (MQ=255)

                            aTCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGAt                                                            <  1:4712896/93‑1 (MQ=255)

                            aTCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGAt                                                            <  1:1415850/93‑1 (MQ=255)

                              cGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGc                                                          <  2:2563860/93‑1 (MQ=255)

                               gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                                                         <  2:2099939/93‑1 (MQ=255)

                               aaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                                                         <  2:1795707/92‑1 (MQ=255)

                                  aaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAAc                                                      >  2:5526882/1‑93 (MQ=255)

                                    agaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTg                                                    >  2:2920504/1‑93 (MQ=255)

                                    agaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTg                                                    >  2:3247770/1‑93 (MQ=255)

                                     gaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTgg                                                   >  1:1893942/1‑93 (MQ=255)

                                     gaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTgg                                                   >  1:6022696/1‑93 (MQ=255)

                                      aaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGa                                                  >  2:1251389/1‑93 (MQ=255)

                                       aaaaCAAATTACAATTTATCTAGATAAAGGGAATATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                                                 >  1:3572765/1‑93 (MQ=255)

                                         aaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGc                                               >  1:4369880/1‑93 (MQ=255)

                                         aaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGc                                               >  2:3892705/1‑93 (MQ=255)

                                           cAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcct                                             <  1:5550793/93‑1 (MQ=255)

                                           cAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcct                                             >  1:6108075/1‑93 (MQ=255)

                                            aaaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctc                                            <  2:4482282/93‑1 (MQ=255)

                                            aaaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctc                                            <  1:5045261/93‑1 (MQ=255)

                                            aaaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctc                                            <  2:5078771/93‑1 (MQ=255)

                                             aaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcc                                           <  1:3310119/93‑1 (MQ=255)

                                              aTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcct                                          >  2:4702668/1‑93 (MQ=255)

                                               ttACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTg                                         >  1:4456054/1‑93 (MQ=255)

                                                 aCAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGaa                                       >  1:6064041/1‑93 (MQ=255)

                                                 aCAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGaa                                       >  1:1775527/1‑93 (MQ=255)

                                                 aCAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGaa                                       >  1:2798482/1‑93 (MQ=255)

                                                  cAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAAc                                      >  1:1865535/1‑93 (MQ=255)

                                                   aaCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACt                                     <  2:4171455/93‑1 (MQ=255)

                                                     cTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACttt                                   >  1:2382146/1‑93 (MQ=255)

                                                      ttATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTg                                  >  2:5851290/1‑93 (MQ=255)

                                                      ttATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTg                                  >  2:4948049/1‑93 (MQ=255)

                                                        atctCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGa                                <  1:1633172/90‑1 (MQ=255)

                                                         tctCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGaa                               <  1:6447909/91‑1 (MQ=255)

                                                          ctCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAAt                              <  2:1126503/92‑1 (MQ=255)

                                                           tCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATc                             <  2:6619533/93‑1 (MQ=255)

                                                           tCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATc                             <  2:2249077/93‑1 (MQ=255)

                                                            cGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCa                            <  2:3542319/93‑1 (MQ=255)

                                                             gATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCAt                           <  1:6233588/93‑1 (MQ=255)

                                                              aTAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATg                          >  2:686873/1‑93 (MQ=255)

                                                              aTAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATg                          >  1:5288272/1‑93 (MQ=255)

                                                              aTAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATg                          >  1:3501787/1‑93 (MQ=255)

                                                               tAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATgg                         >  2:3733089/1‑93 (MQ=255)

                                                                aaaGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGt                        <  1:1350776/93‑1 (MQ=255)

                                                                aaaGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGt                        >  2:2679172/1‑93 (MQ=255)

                                                                aaaGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGt                        <  1:6163938/93‑1 (MQ=255)

                                                                aaaGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGACGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGt                        <  1:3148106/93‑1 (MQ=255)

                                                                 aaGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTa                       >  1:1069219/1‑93 (MQ=255)

                                                                  aGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTAt                      <  1:2576706/93‑1 (MQ=255)

                                                                  aGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTAt                      >  1:5877322/1‑93 (MQ=255)

                                                                  aGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTAt                      >  2:6262617/1‑93 (MQ=255)

                                                                  aGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATAGGTAAAATACAAAGATGCAAACTGGACGCCTCCTGCACTTTCGAATCATGGTAt                      >  1:4019527/1‑93 (MQ=255)

                                                                    ggAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTg                    >  2:2643303/1‑93 (MQ=255)

                                                                      aaaaTGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCt                  >  2:5682084/1‑93 (MQ=255)

                                                                       aaaTGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTg                 >  2:5578371/1‑93 (MQ=255)

                                                                        aaTGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTgg                >  2:2337952/1‑93 (MQ=255)

                                                                         aTGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTggg               <  1:3237490/93‑1 (MQ=255)

                                                                          tGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTgggg              <  2:1847863/93‑1 (MQ=255)

                                                                            cAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCa            <  2:6085627/93‑1 (MQ=255)

                                                                             aaTTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCAt           <  1:2691060/93‑1 (MQ=255)

                                                                              aTTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCAtt          <  1:4876014/93‑1 (MQ=255)

                                                                              aTTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCAtt          >  1:4543254/1‑93 (MQ=255)

                                                                               ttATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCAttt         >  2:1963299/1‑93 (MQ=255)

                                                                                tATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTc        <  2:4842978/93‑1 (MQ=255)

                                                                                tATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTc        <  2:4109936/93‑1 (MQ=255)

                                                                                tATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTc        <  1:3343378/93‑1 (MQ=255)

                                                                                 aTCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCt       <  1:3825467/93‑1 (MQ=255)

                                                                                 aTCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCt       <  1:712598/93‑1 (MQ=255)

                                                                                 aTCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCt       <  2:590002/93‑1 (MQ=255)

                                                                                 aTCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCt       <  1:2186464/93‑1 (MQ=255)

                                                                                 aTCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCt       <  2:6132476/93‑1 (MQ=255)

                                                                                  tCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCtt      >  1:4249647/1‑93 (MQ=255)

                                                                                  tCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCtt      >  2:181031/1‑93 (MQ=255)

                                                                                   ccATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCTTg     <  2:2743134/93‑1 (MQ=255)

                                                                                   ccATATCTGCACCTCAGGCATGGTTAAAATACAAAGAAGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCTTg     <  1:3640160/93‑1 (MQ=255)

                                                                                    cATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCTTGa    >  2:2465334/1‑93 (MQ=255)

                                                                                     atatCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCTTGAc   <  1:5423481/93‑1 (MQ=255)

                                                                                     atatCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCTTGAc   >  2:3472540/1‑93 (MQ=255)

GCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATCATGGTATTGCTGGGGCATTTCTTGACT  >  NC_000913/3185718‑3185896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`