BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,221,280	T→C	L336P (CTG→CCG)	b3076 →	evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,221,280	0	T	C	100.0%	794.1 / NA	243	L336P (CTG→CCG)	b3076	evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (128/115); total (128/115)

Only 100 of 259 total aligned reads displayed.

ACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGC  >  NC_000913/3221190‑3221369

aCGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGAtgccgc                                                                                         >  1:4497233/1‑93 (MQ=255)

 cGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCa                                                                                        >  2:582026/1‑93 (MQ=255)

  gTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCAc                                                                                       <  2:6029235/93‑1 (MQ=255)

  gTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCAc                                                                                       >  2:552/1‑93 (MQ=255)

  gTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCAc                                                                                       >  2:590640/1‑93 (MQ=255)

   ttCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACg                                                                                      >  2:2480340/1‑93 (MQ=255)

    tCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACgg                                                                                     <  1:6440995/93‑1 (MQ=255)

       ggAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGt                                                                                  >  1:4588139/1‑93 (MQ=255)

        gAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTc                                                                                 <  2:5471235/93‑1 (MQ=255)

         aaGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGGCa                                                                                >  2:4393551/1‑93 (MQ=255)

          aGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGGCGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCaa                                                                               <  1:3849545/93‑1 (MQ=255)

          aGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCaa                                                                               <  2:5455549/93‑1 (MQ=255)

          aGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCaa                                                                               <  2:1788204/93‑1 (MQ=255)

           gtggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAAc                                                                              >  1:5293613/1‑93 (MQ=255)

             ggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCg                                                                            >  1:1563151/1‑93 (MQ=255)

             ggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCg                                                                            <  1:3393454/93‑1 (MQ=255)

              gtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGt                                                                           >  1:4056077/1‑93 (MQ=255)

                 ccACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCAc                                                                        >  2:1594311/1‑93 (MQ=255)

                   acaACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGa                                                                      <  1:286718/93‑1 (MQ=255)

                    caACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGAc                                                                     >  1:6329980/1‑93 (MQ=255)

                     aaCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACa                                                                    <  2:83305/93‑1 (MQ=255)

                      aCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGAcca                                                                   >  2:3012875/1‑91 (MQ=255)

                       cgcgTTGGCTTCCGTGATATCAAAGTGCGCGACGTTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAAc                                                                  <  2:172338/93‑1 (MQ=255)

                       cgcgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAAc                                                                  >  2:4099644/1‑93 (MQ=255)

                       cgcgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAAc                                                                  >  2:6429802/1‑93 (MQ=255)

                        gcgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACg                                                                 >  2:6270214/1‑93 (MQ=255)

                        gcgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACg                                                                 <  1:5671604/93‑1 (MQ=255)

                        gcgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGGCAACCGTCACGACAACg                                                                 >  2:3953437/1‑93 (MQ=255)

                         cgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGa                                                                >  1:97861/1‑93 (MQ=255)

                           ttGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatc                                                              <  2:1032619/93‑1 (MQ=255)

                           ttGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatc                                                              >  1:5583644/1‑93 (MQ=255)

                            tGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatca                                                             <  1:691748/93‑1 (MQ=255)

                            tGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatca                                                             <  2:6191984/93‑1 (MQ=255)

                             ggCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatcat                                                            >  2:3593038/1‑93 (MQ=255)

                             ggCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatcat                                                            >  2:4603503/1‑93 (MQ=255)

                              gCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatcatc                                                           <  1:2526535/93‑1 (MQ=255)

                                ttCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGc                                                         >  1:2016302/1‑93 (MQ=255)

                                  ccGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCGCGACAACGATCGTCGCaa                                                       <  1:3896629/93‑1 (MQ=255)

                                       atatCAAAGTGCGCGACGGTCTTTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGcc                                                  <  1:2347252/93‑1 (MQ=255)

                                        tatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcg                                                 >  2:5239305/1‑93 (MQ=255)

                                        tatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcg                                                 >  1:816671/1‑93 (MQ=255)

                                         atCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcgc                                                >  1:1715450/1‑93 (MQ=255)

                                         atCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcgc                                                >  2:1230579/1‑93 (MQ=255)

                                           cAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCgcgc                                              >  2:1975087/1‑93 (MQ=255)

                                            aaaGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCgcgcg                                             >  2:2500853/1‑92 (MQ=255)

                                             aaGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCg                                            <  1:6518936/93‑1 (MQ=255)

                                              aGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGt                                           >  1:2545560/1‑93 (MQ=255)

                                               gTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGtt                                          <  1:482906/93‑1 (MQ=255)

                                               gTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGtt                                          >  1:1807943/1‑93 (MQ=255)

                                                tGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTg                                         >  2:3203269/1‑93 (MQ=255)

                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                      <  2:3872922/93‑1 (MQ=255)

                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                      >  2:5026014/1‑93 (MQ=255)

                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                      >  1:5827523/1‑93 (MQ=255)

                                                    cgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAAt                                     <  2:6368551/93‑1 (MQ=255)

                                                      tcGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCACCCGTCACGACAACGATCATCGCAAAGGCCGCTCCGTTGGAATgg                                   <  1:3192013/92‑1 (MQ=255)

                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATggtt                                 <  1:4205456/93‑3 (MQ=255)

                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                 >  1:2205776/1‑93 (MQ=255)

                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                 <  1:953251/93‑1 (MQ=255)

                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                 >  2:3142854/1‑93 (MQ=255)

                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                 <  1:4368458/93‑1 (MQ=255)

                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                 <  2:2349219/93‑1 (MQ=255)

                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATc                                <  1:4125380/93‑1 (MQ=255)

                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGCCCGCGCCGTTGGAATGGATc                                <  1:5443247/93‑1 (MQ=255)

                                                           cTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATcgc                              >  1:2730494/1‑93 (MQ=255)

                                                           cTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATcgc                              >  2:3791964/1‑93 (MQ=255)

                                                             gTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGt                            >  2:3440381/1‑93 (MQ=255)

                                                             gTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGt                            >  1:5149873/1‑93 (MQ=255)

                                                             gTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGt                            >  1:3762709/1‑93 (MQ=255)

                                                             gTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGt                            >  1:3805410/1‑93 (MQ=255)

                                                              ttCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTc                           >  1:2239048/1‑93 (MQ=255)

                                                              ttCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTc                           <  2:1418390/93‑1 (MQ=255)

                                                              ttCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTc                           <  2:3735489/93‑1 (MQ=255)

                                                               tCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCg                          >  1:855996/1‑93 (MQ=255)

                                                                 tGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCgag                        >  1:3975199/1‑93 (MQ=255)

                                                                 tGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCgag                        <  1:4306676/93‑1 (MQ=255)

                                                                  ggATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCgaga                       >  1:3506572/1‑93 (MQ=255)

                                                                   gATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGaa                      >  1:3185379/1‑93 (MQ=255)

                                                                    aTCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGaaa                     >  2:3165887/1‑93 (MQ=255)

                                                                    aTCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGaaa                     >  1:5196525/1‑93 (MQ=255)

                                                                     tCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAg                    >  1:1937017/1‑93 (MQ=255)

                                                                       aaTAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAt                  <  2:899017/93‑1 (MQ=255)

                                                                       aaTAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAt                  >  1:5228406/1‑93 (MQ=255)

                                                                        aTAACCGTTATGTGATGCCGCACGGCGTGAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtc                 >  1:1080002/1‑93 (MQ=255)

                                                                         tAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtct                <  2:2162221/93‑1 (MQ=255)

                                                                         tAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtcc                >  2:4808674/1‑92 (MQ=255)

                                                                           aCCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTcc              <  2:4518510/93‑1 (MQ=255)

                                                                            ccGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCa             <  2:3236723/93‑1 (MQ=255)

                                                                            ccGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCa             >  2:5669786/1‑93 (MQ=255)

                                                                            ccGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCa             >  1:6110219/1‑93 (MQ=255)

                                                                             cGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAg            <  1:2036217/93‑1 (MQ=255)

                                                                              gTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGt           >  1:4893317/1‑93 (MQ=255)

                                                                               ttATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGtt          <  2:2701235/93‑1 (MQ=255)

                                                                               ttATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGtt          <  1:1576988/93‑1 (MQ=255)

                                                                                 aTGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtga        >  2:4970189/1‑93 (MQ=255)

                                                                                 aTGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtga        >  2:706727/1‑93 (MQ=255)

                                                                                  tgtgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgat       >  1:4690194/1‑93 (MQ=255)

                                                                                   gtgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgatg      >  1:1213605/1‑93 (MQ=255)

                                                                                    tgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgatga     <  2:2832390/93‑1 (MQ=255)

                                                                                     gATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGaa    >  1:925780/1‑93 (MQ=255)

                                                                                       tgccgcACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAagc  >  1:3071036/1‑93 (MQ=255)

ACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGC  >  NC_000913/3221190‑3221369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`