BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	374,060	G→A	E323E (GAG→GAA)	mhpE →	4‑hydroxy‑2‑ketovalerate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	374,060	0	G	A	100.0%	810.1 / NA	217	E323E (GAG→GAA)	mhpE	4‑hydroxy‑2‑ketovalerate aldolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (97/120); total (97/120)

Only 100 of 236 total aligned reads displayed.

TCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAC  >  NC_000913/373970‑374149

tCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAggaaga                                                                                         <  2:4110222/93‑1 (MQ=255)

tCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAggaaga                                                                                         >  2:2136363/1‑93 (MQ=255)

 cACTGTGAAACGGCGGCGGCGCGTTATGGCTTAATTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGat                                                                                        <  1:2625839/93‑1 (MQ=255)

 cACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGat                                                                                        <  1:4976703/93‑1 (MQ=255)

 cACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGat                                                                                        <  2:5680495/93‑1 (MQ=255)

  aCTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGata                                                                                       <  2:346391/93‑1 (MQ=255)

  aCTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGata                                                                                       <  2:4768665/93‑1 (MQ=255)

   cTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTTGCGGCCAGGAAGatat                                                                                      >  1:4139399/1‑93 (MQ=255)

   cTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGatat                                                                                      <  2:2412336/93‑1 (MQ=255)

   cTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGatat                                                                                      >  2:1708145/1‑93 (MQ=255)

     gtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGa                                                                                    >  1:2232220/1‑93 (MQ=255)

     gtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGa                                                                                    >  2:1072225/1‑93 (MQ=255)

      tgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGAt                                                                                   <  1:1352709/93‑1 (MQ=255)

      tgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGAt                                                                                   >  2:3699868/1‑93 (MQ=255)

         aaCGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGt                                                                                >  2:2913360/1‑93 (MQ=255)

         aaCGGCGGCGGCGAGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGt                                                                                <  2:3801029/93‑1 (MQ=255)

           cggcggcggCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTg                                                                              >  1:3796932/1‑93 (MQ=255)

            ggcggcggcGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGa                                                                             >  1:4410639/1‑93 (MQ=255)

             gcggcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGACGATATGATCGTTGAc                                                                            >  2:2762406/1‑93 (MQ=255)

                gcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTg                                                                         >  1:2754766/1‑93 (MQ=255)

                gcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAAAGCCGGATGGTTGGTGGCCAGGAAGATATGATCGTTGACGTg                                                                         >  1:2166643/1‑93 (MQ=255)

                 cggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTgg                                                                        <  1:5925858/93‑1 (MQ=255)

                 cggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTgg                                                                        <  2:3569197/93‑1 (MQ=255)

                  ggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgc                                                                       <  1:1655118/93‑1 (MQ=255)

                   gcgcGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgcg                                                                      <  2:4019874/93‑1 (MQ=255)

                    cgcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgcgc                                                                     <  1:4677430/93‑1 (MQ=255)

                    cgcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgcgc                                                                     <  1:1645059/93‑1 (MQ=255)

                     gcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCt                                                                    >  2:2224668/1‑93 (MQ=255)

                       gTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTgg                                                                  >  2:2622253/1‑93 (MQ=255)

                         tATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGAt                                                                <  1:6392895/93‑1 (MQ=255)

                          aTGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATc                                                               <  2:455257/93‑1 (MQ=255)

                          aTGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATc                                                               <  1:107530/93‑1 (MQ=255)

                          aTGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATc                                                               <  2:4781678/93‑1 (MQ=255)

                            ggCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTg                                                             <  1:3183826/93‑1 (MQ=255)

                              cTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTgcg                                                           >  2:1384456/1‑93 (MQ=255)

                                tAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGca                                                         >  1:2034993/1‑93 (MQ=255)

                                 aaGTGCGGTGGATATTCTCGTTGAGCTGGGAAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaa                                                        >  1:2637940/1‑93 (MQ=255)

                                  aGTGGGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaac                                                       <  1:5759914/93‑1 (MQ=255)

                                  aGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaac                                                       >  2:3188169/1‑93 (MQ=255)

                                  aGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaac                                                       >  1:3528243/1‑93 (MQ=255)

                                  aGTGCGGTGAATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaac                                                       <  1:1470145/93‑1 (MQ=255)

                                   gTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaaca                                                      >  2:6579666/1‑93 (MQ=255)

                                   gTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaaca                                                      >  2:2445205/1‑93 (MQ=255)

                                     gCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaacaac                                                    <  2:2781448/93‑1 (MQ=255)

                                        gTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACaaa                                                 <  1:1867311/93‑1 (MQ=255)

                                        gTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACaaa                                                 <  1:359772/93‑1 (MQ=255)

                                        gTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACaaa                                                 <  2:4124695/93‑1 (MQ=255)

                                         tGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaat                                                <  1:3967501/93‑1 (MQ=255)

                                         tGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaat                                                <  1:1914853/93‑1 (MQ=255)

                                         tGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaat                                                >  1:2548857/1‑93 (MQ=255)

                                          ggATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaata                                               >  1:4261962/1‑93 (MQ=255)

                                          ggATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaata                                               >  2:5264100/1‑93 (MQ=255)

                                             tatTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatg                                            >  2:2177286/1‑93 (MQ=255)

                                              atTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatga                                           <  2:2823667/93‑1 (MQ=255)

                                              atTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatga                                           <  2:343870/93‑1 (MQ=255)

                                              atTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatga                                           <  1:5222523/93‑1 (MQ=255)

                                               ttCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatgat                                          <  2:1934540/93‑1 (MQ=255)

                                               ttCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatgat                                          <  2:878072/93‑1 (MQ=255)

                                                 ctcGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAAtgatga                                        >  2:1294964/1‑93 (MQ=255)

                                                  tcGTTGAGCTGGGCAAACGCCGGATTGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGAc                                       <  1:4758988/93‑1 (MQ=255)

                                                    gTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTg                                     >  1:2631608/1‑93 (MQ=255)

                                                     ttGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGc                                    >  2:4363325/1‑93 (MQ=255)

                                                      tGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGcc                                   >  2:2265206/1‑93 (MQ=255)

                                                       gAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCg                                  <  2:4583995/93‑1 (MQ=255)

                                                        aGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCga                                 >  2:1470275/1‑93 (MQ=255)

                                                         gCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCgag                                <  2:1108590/93‑1 (MQ=255)

                                                            gggCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAAc                             >  1:1228517/1‑93 (MQ=255)

                                                             ggCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACg                            <  1:2801909/93‑1 (MQ=255)

                                                               cAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTg                          <  1:4867120/93‑1 (MQ=255)

                                                               cAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTg                          <  2:3882940/93‑1 (MQ=255)

                                                                aaaCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGc                         >  1:4041002/1‑93 (MQ=255)

                                                                  aCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAt                       <  2:323385/93‑1 (MQ=255)

                                                                  aCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAt                       <  2:1007355/93‑1 (MQ=255)

                                                                  aCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAt                       >  1:3756411/1‑93 (MQ=255)

                                                                  aCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAt                       <  1:6158793/93‑1 (MQ=255)

                                                                    gCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGCACGTGAAttt                     >  1:3834728/1‑93 (MQ=255)

                                                                    gCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAttt                     >  1:579761/1‑93 (MQ=255)

                                                                    gCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAttt                     <  2:4999986/93‑1 (MQ=255)

                                                                    gCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAttt                     >  1:6239920/1‑93 (MQ=255)

                                                                     ccGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAtttt                    <  1:458911/93‑1 (MQ=255)

                                                                       ggATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGt                  >  1:104075/1‑93 (MQ=255)

                                                                       ggATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGt                  >  2:1435482/1‑93 (MQ=255)

                                                                        gATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTa                 >  1:571697/1‑93 (MQ=255)

                                                                        gATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTa                 >  2:918655/1‑93 (MQ=255)

                                                                         aTGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTAt                >  2:6526971/1‑93 (MQ=255)

                                                                         aTGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTAt                <  1:4830348/93‑1 (MQ=255)

                                                                          tGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATg               >  2:6660791/1‑93 (MQ=255)

                                                                           ggTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGc              >  1:1998932/1‑93 (MQ=255)

                                                                            gTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGcc             >  1:1698809/1‑93 (MQ=255)

                                                                            gTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGcc             >  1:4440985/1‑93 (MQ=255)

                                                                            gTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGcc             >  2:2982429/1‑93 (MQ=255)

                                                                             ttGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGccc            >  2:5387117/1‑93 (MQ=255)

                                                                                gcggcCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATc         >  1:179595/1‑93 (MQ=255)

                                                                                 cggcCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATcc        >  2:6281080/1‑93 (MQ=255)

                                                                                 cggcCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATcc        <  1:5661981/93‑1 (MQ=255)

                                                                                   gcCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCa      <  1:760974/93‑1 (MQ=255)

                                                                                     cAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGc    >  2:5923758/1‑93 (MQ=255)

                                                                                      aggaagATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCa   >  1:1286852/1‑93 (MQ=255)

                                                                                      aggaagATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCa   >  2:2343761/1‑93 (MQ=255)

                                                                                       ggaagaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAc  >  2:1522640/1‑93 (MQ=255)

TCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAC  >  NC_000913/373970‑374149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`