BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,251,140	G→C	L239V (CTT→GTT)	yhaJ ←	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,251,140	0	G	C	100.0%	416.6 / NA	129	L239V (CTT→GTT)	yhaJ	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (85/44); total (85/44)

Only 100 of 135 total aligned reads displayed.

TCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGC  >  NC_000913/3251050‑3251229

tctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAccc                                                                                         >  2:241444/1‑93 (MQ=255)

tctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAccc                                                                                         >  2:382143/1‑93 (MQ=255)

tctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAccc                                                                                         >  1:273620/1‑93 (MQ=255)

  tcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGCCCATAGGATACGGCATCGTCGCCACGCCAACcccc                                                                                       <  1:599983/93‑1 (MQ=255)

  tcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACcccc                                                                                       >  1:3720177/1‑93 (MQ=255)

  tcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACcccc                                                                                       >  1:5516287/1‑93 (MQ=255)

  tcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACcccc                                                                                       >  1:1110498/1‑93 (MQ=255)

    gCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGc                                                                                     >  1:6293003/1‑93 (MQ=255)

     cTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGcc                                                                                    >  1:5473371/1‑93 (MQ=255)

      tGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCa                                                                                   >  1:3839817/1‑93 (MQ=255)

      tGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCa                                                                                   >  1:797550/1‑93 (MQ=255)

          cGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTaa                                                                               >  1:3473043/1‑93 (MQ=255)

           gATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAAt                                                                              <  2:3685843/93‑1 (MQ=255)

            aTTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAAtc                                                                             >  2:5453847/1‑92 (MQ=255)

             ttCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGc                                                                            <  2:5783442/93‑1 (MQ=255)

              tCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGcc                                                                           <  1:4800635/93‑1 (MQ=255)

                cGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTg                                                                         <  2:2426375/93‑1 (MQ=255)

                cGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTg                                                                         >  1:3467835/1‑93 (MQ=255)

                 gggCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGa                                                                        >  2:3220710/1‑93 (MQ=255)

                  ggCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGAc                                                                       <  2:197935/93‑1 (MQ=255)

                     tGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGtt                                                                    <  2:6256440/93‑1 (MQ=255)

                     tGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGtt                                                                    >  2:3323939/1‑93 (MQ=255)

                      gacgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGttt                                                                   <  2:433133/93‑1 (MQ=255)

                      gacgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGttt                                                                   <  2:244917/93‑1 (MQ=255)

                       acgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTa                                                                  <  2:3899005/93‑1 (MQ=255)

                       acgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTa                                                                  >  1:5408060/1‑93 (MQ=255)

                         gacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATc                                                                >  1:2708322/1‑93 (MQ=255)

                         gacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATc                                                                <  2:4214218/93‑1 (MQ=255)

                          acacGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCt                                                               >  1:2489251/1‑93 (MQ=255)

                          acacGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCt                                                               >  2:4615440/1‑93 (MQ=255)

                            acGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCCTCGTCGCCACGCCAACCCCCGCCATTAATGCCTGACGTTTATCTTc                                                             >  1:1966410/1‑93 (MQ=255)

                            acGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTc                                                             <  1:1168659/93‑1 (MQ=255)

                            acGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTc                                                             >  2:4807647/1‑93 (MQ=255)

                            acGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTc                                                             >  1:22624/1‑93 (MQ=255)

                              gCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCaa                                                           >  1:5222709/1‑93 (MQ=255)

                               cAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAAt                                                          >  1:5159751/1‑93 (MQ=255)

                                aaCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATc                                                         >  2:380280/1‑93 (MQ=255)

                                 aCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCg                                                        >  1:1152/1‑93 (MQ=255)

                                 aCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCg                                                        >  1:873582/1‑93 (MQ=255)

                                 aCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCg                                                        >  2:4968457/1‑93 (MQ=255)

                                  ccGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGt                                                       >  1:3872207/1‑93 (MQ=255)

                                  ccGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGt                                                       >  1:1246569/1‑93 (MQ=255)

                                   cGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTg                                                      >  2:5783120/1‑93 (MQ=255)

                                     cccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCt                                                    >  2:5602554/1‑93 (MQ=255)

                                      ccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTc                                                   >  1:1263350/1‑93 (MQ=255)

                                       cTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCa                                                  >  1:234698/1‑93 (MQ=255)

                                       cTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCa                                                  >  2:4955249/1‑93 (MQ=255)

                                        ttCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCAc                                                 >  2:3480145/1‑93 (MQ=255)

                                          ccGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCg                                               >  2:6376861/1‑93 (MQ=255)

                                            gCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGtt                                             <  1:5720118/93‑1 (MQ=255)

                                            gCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGtt                                             >  1:553802/1‑93 (MQ=255)

                                               atatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAg                                          >  1:2116640/1‑93 (MQ=255)

                                               atatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAg                                          >  2:6384766/1‑93 (MQ=255)

                                                 atCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgcg                                        >  1:2212395/1‑93 (MQ=255)

                                                  tCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgcgc                                       >  2:1054045/1‑93 (MQ=255)

                                                    tttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCgg                                     <  2:6679891/93‑1 (MQ=255)

                                                    tttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCgg                                     <  1:2162355/93‑1 (MQ=255)

                                                     ttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGc                                    <  2:4856000/93‑1 (MQ=255)

                                                     ttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGc                                    >  2:5013313/1‑93 (MQ=255)

                                                      tttCGACCATAGGATCCGCCACCGTCGCCCCCCTATCCCCCGCCAGTAATGCCTGACGTTAATCTTCAATCGTGCTCACCGTTAAGCGCGGCt                                   <  2:3514266/93‑1 (MQ=255)

                                                      tttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCt                                   >  1:69820/1‑93 (MQ=255)

                                                        tCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGt                                 >  2:1689374/1‑93 (MQ=255)

                                                         cGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGtt                                <  2:2074037/93‑1 (MQ=255)

                                                         cGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGtt                                <  1:1422680/93‑1 (MQ=255)

                                                         cGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGtt                                <  1:1718468/93‑1 (MQ=255)

                                                            ccATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGt                             >  2:686414/1‑93 (MQ=255)

                                                            ccATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGt                             >  2:108064/1‑93 (MQ=255)

                                                             cATAGGATACGGCATCGTCGCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTc                            <  2:4847257/93‑1 (MQ=255)

                                                              aTAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTcc                           >  1:4828480/1‑93 (MQ=255)

                                                               tAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCca                          >  2:4265150/1‑93 (MQ=255)

                                                                 ggATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagc                        >  1:293639/1‑93 (MQ=255)

                                                                  gATGCGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagca                       <  1:1987611/93‑1 (MQ=255)

                                                                  gATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTCAGCGCGGCTGTTTCTCcagcc                       >  1:2742691/1‑92 (MQ=255)

                                                                  gATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagca                       >  2:2015694/1‑93 (MQ=255)

                                                                  gATACGGCATCGCCGCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagca                       <  1:36893/93‑1 (MQ=255)

                                                                    tACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCagcagc                     >  2:2292882/1‑93 (MQ=255)

                                                                    tACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCagcagc                     <  2:6575156/93‑1 (MQ=255)

                                                                    tACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGAGCGGCTGTTTGTCCagcagc                     >  1:6000750/1‑93 (MQ=255)

                                                                      cGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGCCCAGCAGCTg                   <  2:2379639/93‑1 (MQ=255)

                                                                       ggCATCGTCGCCACGCCAACCCCAGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCTAGCAGCTGt                  >  1:1952619/1‑93 (MQ=255)

                                                                        gCATCGTCGCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTa                 <  1:5969521/93‑1 (MQ=255)

                                                                        gCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTa                 >  1:2606777/1‑93 (MQ=255)

                                                                        gCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTa                 >  1:532983/1‑93 (MQ=255)

                                                                        gCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTa                 >  1:5776487/1‑93 (MQ=255)

                                                                         cATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTAc                >  1:5401243/1‑93 (MQ=255)

                                                                          ctcgtccCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACg               <  2:1927213/92‑1 (MQ=255)

                                                                          aTCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACg               >  1:1252093/1‑93 (MQ=255)

                                                                            cgtcgCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGt             <  1:5711418/93‑1 (MQ=255)

                                                                            cgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGt             <  2:2430273/93‑1 (MQ=255)

                                                                             gtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTc            >  1:1393053/1‑93 (MQ=255)

                                                                             gtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTc            >  1:4952519/1‑93 (MQ=255)

                                                                              tcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCa           >  2:5415733/1‑93 (MQ=255)

                                                                               cgccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCaa          >  1:227672/1‑93 (MQ=255)

                                                                               cgccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCaa          >  2:642903/1‑93 (MQ=255)

                                                                               cgccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCaa          >  2:6520039/1‑93 (MQ=255)

                                                                                gccacgccacCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAac         <  2:2843678/93‑1 (MQ=255)

                                                                                 ccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAaca        >  2:3184356/1‑93 (MQ=255)

                                                                                  cacgccaCCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAacac       <  1:3568775/93‑1 (MQ=255)

                                                                                    cgccaCCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCg     <  1:3919428/93‑1 (MQ=255)

                                                                                       caACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGgc  <  2:5399323/93‑1 (MQ=255)

TCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGC  >  NC_000913/3251050‑3251229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`