BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,277,670	T→C	R372R (CGT→CGC)	agaZ →	putative tagatose‑6‑phosphate kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,670	0	T	C	100.0%	886.9 / NA	270	R372R (CGT→CGC)	agaZ	putative tagatose‑6‑phosphate kinase 2
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (142/128); total (142/128)

Only 100 of 276 total aligned reads displayed.

TCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCC  >  NC_000913/3277580‑3277759

 cgtcTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAAt                                                                                        >  2:1765898/1‑93 (MQ=255)

  gtcTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATc                                                                                       <  2:4972886/93‑1 (MQ=255)

  gtcTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATc                                                                                       >  1:542749/1‑93 (MQ=255)

  gtcTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATc                                                                                       <  1:23691/93‑1 (MQ=255)

  gtcTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATc                                                                                       >  1:6443487/1‑93 (MQ=255)

   tcTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCt                                                                                      <  2:3905087/93‑1 (MQ=255)

   tcTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCt                                                                                      >  2:2399400/1‑93 (MQ=255)

     tGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTgg                                                                                    <  2:1126713/93‑1 (MQ=255)

      ggCGCGTGTTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGc                                                                                   <  1:1047263/93‑1 (MQ=255)

       gcgcGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCg                                                                                  <  1:1299184/93‑1 (MQ=255)

       gcgcGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCg                                                                                  >  2:6217411/1‑93 (MQ=255)

         gcgTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGa                                                                                <  1:5762236/93‑1 (MQ=255)

         gcgTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGa                                                                                >  1:5532189/1‑93 (MQ=255)

              gTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCAc                                                                           >  2:362846/1‑93 (MQ=255)

               ttATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCAcc                                                                          <  2:1623413/93‑1 (MQ=255)

                tataGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCa                                                                         <  1:211085/93‑1 (MQ=255)

                 ataGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCaa                                                                        >  2:2863457/1‑93 (MQ=255)

                 ataGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCaa                                                                        <  2:1999487/93‑1 (MQ=255)

                 ataGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCaa                                                                        <  1:3925013/93‑1 (MQ=255)

                 ataGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCaa                                                                        <  1:2035120/93‑1 (MQ=255)

                 ataGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCaa                                                                        <  1:2787649/93‑1 (MQ=255)

                  taGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAAt                                                                       >  2:826492/1‑93 (MQ=255)

                  taGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAAt                                                                       <  1:4472668/93‑1 (MQ=255)

                   aGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAAtt                                                                      <  1:1427271/93‑1 (MQ=255)

                   aGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAAtt                                                                      <  1:4275253/93‑1 (MQ=255)

                    gCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTc                                                                     >  1:1888516/1‑93 (MQ=255)

                    gCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTc                                                                     >  2:435290/1‑93 (MQ=255)

                     cTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTcc                                                                    >  2:3691392/1‑93 (MQ=255)

                     cTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTcc                                                                    <  2:464677/93‑1 (MQ=255)

                       aCTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgc                                                                  >  1:243399/1‑93 (MQ=255)

                        cTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgct                                                                 >  2:5741367/1‑93 (MQ=255)

                        cTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgct                                                                 <  1:3600567/93‑1 (MQ=255)

                        cTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgct                                                                 <  2:2244356/93‑1 (MQ=255)

                         tCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgctg                                                                <  1:2418508/93‑1 (MQ=255)

                         tCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgctg                                                                >  2:1448602/1‑93 (MQ=255)

                           ggATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgctgcc                                                              >  1:178951/1‑93 (MQ=255)

                            gATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgctgccg                                                             >  2:6407544/1‑93 (MQ=255)

                            gATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgctgccg                                                             >  1:4213061/1‑93 (MQ=255)

                             aTCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgctgccgc                                                            >  1:5013467/1‑93 (MQ=255)

                             aTCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgctgccgc                                                            <  2:5527472/93‑1 (MQ=255)

                               cgcgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTccgctgccgctg                                                          <  1:4511450/93‑1 (MQ=255)

                                 cgTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGAt                                                        <  1:6352117/93‑1 (MQ=255)

                                  gTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATc                                                       <  2:127326/93‑1 (MQ=255)

                                  gTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATc                                                       <  2:3364883/93‑1 (MQ=255)

                                   tGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCa                                                      <  2:1529326/93‑1 (MQ=255)

                                    gcgcTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAg                                                     <  1:5344058/93‑1 (MQ=255)

                                       cTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCa                                                  <  2:3539057/93‑1 (MQ=255)

                                       cTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCa                                                  <  1:3172713/93‑1 (MQ=255)

                                        tATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAg                                                 >  2:896142/1‑93 (MQ=255)

                                        tATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAg                                                 >  1:169393/1‑93 (MQ=255)

                                        tATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAg                                                 >  1:521090/1‑93 (MQ=255)

                                          ttACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTa                                               <  1:6507979/93‑1 (MQ=255)

                                           tACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTAt                                              <  2:2477093/93‑1 (MQ=255)

                                           tACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTAt                                              <  2:6603133/93‑1 (MQ=255)

                                           tACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTAt                                              >  1:6112478/1‑93 (MQ=255)

                                           tACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTAt                                              >  1:3817785/1‑93 (MQ=255)

                                              tGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTg                                           <  2:1162371/93‑1 (MQ=255)

                                              tGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTg                                           >  1:5863955/1‑93 (MQ=255)

                                              tGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTg                                           >  1:998520/1‑93 (MQ=255)

                                               ggCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGc                                          >  2:5123122/1‑93 (MQ=255)

                                               ggCAGGACAGCCAGATTGACGAAGCTTTCACTCATCTGGTACGCAATCTGGCTGATTCACCAATTCCAATGCCGCTGATCAGCCAGTATCTGc                                          <  1:3098792/93‑1 (MQ=255)

                                                  cGGACAGCCAGATTGATGACTCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACTAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGc                                       <  2:5678969/93‑1 (MQ=255)

                                                  cGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGc                                       <  2:4187203/93‑1 (MQ=255)

                                                  cGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGc                                       <  2:2065678/93‑1 (MQ=255)

                                                  cGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGc                                       >  1:1114646/1‑93 (MQ=255)

                                                     aCAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGc                                    <  2:4047882/93‑1 (MQ=255)

                                                      cAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCa                                   >  2:214923/1‑93 (MQ=255)

                                                       aGCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAg                                  <  1:5065754/93‑1 (MQ=255)

                                                        gCCAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGt                                 >  1:5153489/1‑93 (MQ=255)

                                                        cccAGATTGATGACGCTTTCCCTCATCTGTTACGCAATCTGTCGGATTCACCAATACCGCTGCCGCTGATCAGCCAGTATCTGCCTCTGCAGt                                 <  1:6204826/92‑1 (MQ=255)

                                                          cAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTAc                               <  2:1401845/93‑1 (MQ=255)

                                                          cAGATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTAc                               >  2:1257720/1‑93 (MQ=255)

                                                            gATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGt                             >  1:2258749/1‑93 (MQ=255)

                                                            gATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGt                             <  1:5593519/93‑1 (MQ=255)

                                                            gATTGATGACGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGt                             >  2:5757466/1‑93 (MQ=255)

                                                               tgatgaCGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGaa                          <  2:6490962/93‑1 (MQ=255)

                                                                 atgaCGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAg                        <  2:4794298/93‑1 (MQ=255)

                                                                   gaCGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGtt                      >  1:3308964/1‑93 (MQ=255)

                                                                   gaCGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGtt                      <  1:3510087/93‑1 (MQ=255)

                                                                    aCGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTc                     <  2:6543985/93‑1 (MQ=255)

                                                                     cGCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCg                    <  2:3027312/93‑1 (MQ=255)

                                                                      gCTTTCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGc                   >  1:6305657/1‑93 (MQ=255)

                                                                       cTTTCGCTCATCTGGTACGCAATCTGCCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCt                  <  1:4755531/93‑1 (MQ=255)

                                                                        tttCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTc                 <  2:5278039/93‑1 (MQ=255)

                                                                        tttCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTc                 <  2:4437626/93‑1 (MQ=255)

                                                                         ttCGCTCATCTCGTACGCAATCTGGCGCATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTcc                <  1:4101483/93‑1 (MQ=255)

                                                                          tCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCg               >  1:876647/1‑93 (MQ=255)

                                                                          tCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCg               >  1:81463/1‑93 (MQ=255)

                                                                          tCGCTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCg               <  2:3277430/93‑1 (MQ=255)

                                                                             cTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCg            <  1:3797243/93‑1 (MQ=255)

                                                                             cTCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCg            <  1:678595/93‑1 (MQ=255)

                                                                              tCATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGa           >  1:889533/1‑93 (MQ=255)

                                                                              gcATCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGa           <  1:222866/92‑1 (MQ=255)

                                                                                aTCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGc         <  2:319628/93‑1 (MQ=255)

                                                                                aTCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGc         >  1:2530006/1‑93 (MQ=255)

                                                                                aTCTGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCAGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGc         >  2:1797910/1‑93 (MQ=255)

                                                                                   tGGTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGc      >  1:3632038/1‑93 (MQ=255)

                                                                                    ggTACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCa     >  1:2963558/1‑93 (MQ=255)

                                                                                      tACGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGc   >  2:2984505/1‑93 (MQ=255)

                                                                                       aCGCAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGcc  <  1:6041636/93‑1 (MQ=255)

TCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCC  >  NC_000913/3277580‑3277759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`