BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,295,175	T→C	Q202R (CAG→CGG)	yraQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,295,175	0	T	C	99.5%	623.8 / ‑4.7	191	Q202R (CAG→CGG)	yraQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (87/103); minor base A (1/0); total (88/103)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01

Only 100 of 199 total aligned reads displayed.

ATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCA  >  NC_000913/3295085‑3295264

aTCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACGCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGc                                                                                         >  2:2552855/1‑93 (MQ=255)

  cGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGt                                                                                       >  1:732081/1‑93 (MQ=255)

   gTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGtt                                                                                      <  1:4275413/93‑1 (MQ=255)

   gTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGtt                                                                                      <  1:3505163/93‑1 (MQ=255)

   gTACTCCAGAAAAGCGTCCATAGAGCCCTGCCCCAGAGGGTAAAAAACCAGACCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGtt                                                                                      >  2:140254/1‑93 (MQ=255)

    tACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGttt                                                                                     <  2:1211777/93‑1 (MQ=255)

    tACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGttt                                                                                     <  2:3659930/93‑1 (MQ=255)

     aCTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTg                                                                                    <  1:1425227/93‑1 (MQ=255)

       tCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAACCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCg                                                                                  <  1:3630722/93‑1 (MQ=255)

        cgaGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCgg                                                                                 <  1:1016107/91‑1 (MQ=255)

        ccAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCgg                                                                                 <  2:1229577/93‑1 (MQ=255)

         cAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGgt                                                                                <  2:2267411/93‑1 (MQ=255)

         cAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGgt                                                                                <  1:4091682/93‑1 (MQ=255)

         cAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGgt                                                                                <  1:1533476/93‑1 (MQ=255)

         cAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGgt                                                                                <  1:157526/93‑1 (MQ=255)

           gAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGgtgt                                                                              <  1:4620756/93‑1 (MQ=255)

           gAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGgtgt                                                                              >  2:3891544/1‑93 (MQ=255)

           gAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCACGTTTGCGgtgt                                                                              >  1:5386832/1‑93 (MQ=255)

            gaaaGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGtt                                                                             <  2:6436863/92‑1 (MQ=255)

            aaaaGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGtt                                                                             <  1:573650/93‑1 (MQ=255)

             aaaGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGttt                                                                            <  1:1334193/93‑1 (MQ=255)

             aaaGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGttt                                                                            <  2:61713/93‑1 (MQ=255)

              aaGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTc                                                                           >  2:4086891/1‑93 (MQ=255)

              aaGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTc                                                                           >  1:2965651/1‑93 (MQ=255)

              aaGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTc                                                                           >  2:5714691/1‑93 (MQ=255)

               aGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCa                                                                          >  1:890248/1‑93 (MQ=255)

                gCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAGCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCAc                                                                         <  1:3041310/93‑1 (MQ=255)

                  gTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGc                                                                       >  2:614279/1‑93 (MQ=255)

                   tCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCa                                                                      >  2:4622257/1‑93 (MQ=255)

                      aTAGCGCCCTGCCCCAGCGGCTAAAAACCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCAccc                                                                   <  1:1466559/93‑1 (MQ=255)

                      aTAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCAccc                                                                   >  2:5802549/1‑93 (MQ=255)

                       tAGCGCCCTGCCCCAGCGGCTAAAAACCCCGCCCTGTGCTTCCGGTATGTCAACTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCa                                                                  <  2:4819179/93‑1 (MQ=255)

                        aGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAt                                                                 >  1:821344/1‑93 (MQ=255)

                        aGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAt                                                                 <  1:5746377/93‑1 (MQ=255)

                        aGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAt                                                                 <  1:275390/93‑1 (MQ=255)

                         gcgcCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAtt                                                                <  2:5230628/93‑1 (MQ=255)

                         gcgcCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAtt                                                                <  1:5844043/93‑1 (MQ=255)

                          cgcCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAttt                                                               <  2:3506378/93‑1 (MQ=255)

                            cccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCCCCCAttttt                                                             >  2:5177931/1‑93 (MQ=255)

                            cccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAttttt                                                             <  1:3128214/93‑1 (MQ=255)

                                 ccctAGCGGCTAAAAACCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCAcc                                                        <  1:5131074/93‑1 (MQ=255)

                                 ccccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGGGTTTGCGGTGTTTCACGCACCCATTTTTGCAcc                                                        >  1:1726567/1‑93 (MQ=255)

                                 ccccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCAcc                                                        >  2:1418846/1‑93 (MQ=255)

                                 ccccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCAcc                                                        <  2:6687861/93‑1 (MQ=255)

                                 ccccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCAcc                                                        <  2:5367485/93‑1 (MQ=255)

                                 ccccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCAcc                                                        <  2:877795/93‑1 (MQ=255)

                                  cccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCa                                                       <  1:6141124/93‑1 (MQ=255)

                                  cccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCa                                                       >  2:3340097/1‑93 (MQ=255)

                                  cccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCa                                                       <  2:244765/93‑1 (MQ=255)

                                   ccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAg                                                      >  1:640684/1‑93 (MQ=255)

                                   ccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAg                                                      <  1:5424416/93‑1 (MQ=255)

                                    cAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGc                                                     <  1:3659980/93‑1 (MQ=255)

                                     aGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCg                                                    <  1:1591942/93‑1 (MQ=255)

                                       cGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTc                                                  <  1:1284516/93‑1 (MQ=255)

                                        ggCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCg                                                 >  2:4178762/1‑93 (MQ=255)

                                        ggCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTCTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCg                                                 >  1:22708/1‑93 (MQ=255)

                                         gCTAAAAATCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGc                                                <  2:6330763/93‑1 (MQ=255)

                                         gCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGc                                                <  1:243853/93‑1 (MQ=255)

                                             aaaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATc                                            >  2:2159419/1‑93 (MQ=255)

                                             aaaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATc                                            <  2:4330417/93‑1 (MQ=255)

                                             aaaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATc                                            <  2:5463980/93‑1 (MQ=255)

                                             aaaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATc                                            <  2:4013510/93‑1 (MQ=255)

                                             aaaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATc                                            >  1:1646875/1‑93 (MQ=255)

                                                accccGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGc                                         <  2:5003929/91‑1 (MQ=255)

                                                   ccGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAac                                      <  1:392599/93‑1 (MQ=255)

                                                   ccGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCAGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAac                                      >  1:5504824/1‑93 (MQ=255)

                                                    cGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAaca                                     >  2:1944826/1‑93 (MQ=255)

                                                    cGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAaca                                     >  2:4538424/1‑93 (MQ=255)

                                                      cccTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACAcc                                   >  1:1058495/1‑93 (MQ=255)

                                                       ccTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCa                                  >  1:3693133/1‑93 (MQ=255)

                                                       ccTGTGCTTCCGGTATGTCAATCTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCa                                  <  1:2550725/93‑1 (MQ=255)

                                                        cTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCAt                                 >  2:3308873/1‑93 (MQ=255)

                                                        cTGTGCTTCCGGTATGTCAATTTCGACCGGGGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCAt                                 >  2:1492560/1‑93 (MQ=255)

                                                         tgtgCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATc                                >  1:5146067/1‑93 (MQ=255)

                                                           tgCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCAc                              <  1:536335/93‑1 (MQ=255)

                                                            gCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCAcc                             >  2:5563300/1‑93 (MQ=255)

                                                             cTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCa                            <  1:4967422/93‑1 (MQ=255)

                                                             cTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCa                            >  2:744292/1‑93 (MQ=255)

                                                              ttCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAg                           <  1:1040160/93‑1 (MQ=255)

                                                                ccGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGcc                         >  1:2366720/1‑93 (MQ=255)

                                                                   gTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCgg                      >  2:3844354/1‑93 (MQ=255)

                                                                     aTGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGcc                    >  2:804490/1‑93 (MQ=255)

                                                                      tGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGcca                   >  2:6047354/1‑93 (MQ=255)

                                                                       gTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGccac                  >  2:4901323/1‑93 (MQ=255)

                                                                       gTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGccac                  <  2:6582559/93‑1 (MQ=255)

                                                                        tCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGccacc                 <  2:4463984/93‑1 (MQ=255)

                                                                        tCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGccacc                 <  2:2317129/93‑1 (MQ=255)

                                                                           aTTTCGACCGGAGCCCGCGTTTGCGGTGGTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGa              >  1:2530609/1‑93 (MQ=255)

                                                                            tttCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGAc             >  2:2829996/1‑93 (MQ=255)

                                                                             ttCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACg            <  1:2092732/93‑1 (MQ=255)

                                                                                gACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAAt         <  1:6596941/93‑1 (MQ=255)

                                                                                 aCCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAAtg        >  1:1921107/1‑92 (MQ=255)

                                                                                 aCCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATc        <  2:4917915/93‑1 (MQ=255)

                                                                                 aCCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATc        >  2:1710718/1‑93 (MQ=255)

                                                                                 aCCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATc        <  2:2136775/93‑1 (MQ=255)

                                                                                 aCCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATc        <  2:2666011/93‑1 (MQ=255)

                                                                                 aCCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATc        <  1:1485305/93‑1 (MQ=255)

                                                                                  ccGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATcg       >  2:5901544/1‑93 (MQ=255)

                                                                                      tGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATcgccgc   >  1:3456998/1‑93 (MQ=255)

                                                                                       gCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCa  <  2:138121/93‑1 (MQ=255)

ATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCA  >  NC_000913/3295085‑3295264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`