BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,305,459	C→T	A32T (GCT→ACT)	deaD ←	inducible ATP‑independent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,305,459	0	C	T	100.0%	1108.2 / NA	311	A32T (GCT→ACT)	deaD	inducible ATP‑independent RNA helicase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (158/153); total (158/153)

Only 100 of 313 total aligned reads displayed.

CGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCA  >  NC_000913/3305369‑3305548

cgcgGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCt                                                                                         <  2:5283966/93‑1 (MQ=255)

 gcgGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCtt                                                                                        >  2:5374021/1‑93 (MQ=255)

 gcgGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCtt                                                                                        <  1:703785/93‑1 (MQ=255)

  cgGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGTCTTCAAGGATAGGAGTCTTc                                                                                       <  1:2017601/93‑1 (MQ=255)

  cgGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTc                                                                                       <  1:4434431/93‑1 (MQ=255)

  cgGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTc                                                                                       <  1:4637278/93‑1 (MQ=255)

    gCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAg                                                                                     <  2:2071481/93‑1 (MQ=255)

     ccATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAgg                                                                                    >  2:491155/1‑93 (MQ=255)

       aTTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGcc                                                                                  >  2:1542276/1‑93 (MQ=255)

       aTTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGcc                                                                                  <  1:6647316/93‑1 (MQ=255)

       aTTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGcc                                                                                  >  1:2130902/1‑93 (MQ=255)

       aTTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGcc                                                                                  >  2:3273212/1‑93 (MQ=255)

        ttCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGccc                                                                                 >  2:6188571/1‑93 (MQ=255)

        ttCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGccc                                                                                 <  1:940618/93‑1 (MQ=255)

        ttCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGccc                                                                                 <  1:5545692/93‑1 (MQ=255)

           agcagATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTACGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGa                                                                              >  2:4699131/1‑93 (MQ=255)

             cagATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTTTTCAGGCCCAGATc                                                                            <  1:4065957/93‑1 (MQ=255)

               gATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTg                                                                          >  1:6423708/1‑93 (MQ=255)

                 tgtgGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCa                                                                        >  1:3280093/1‑93 (MQ=255)

                  gtgGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCaa                                                                       >  2:1985539/1‑93 (MQ=255)

                  gtgGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCaa                                                                       >  1:627628/1‑93 (MQ=255)

                   tgGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCaaa                                                                      >  1:4990414/1‑93 (MQ=255)

                    ggAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCaaaa                                                                     >  1:2617750/1‑93 (MQ=255)

                    ggAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCaaaa                                                                     >  2:769672/1‑93 (MQ=255)

                    ggAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCaaaa                                                                     >  2:3945960/1‑93 (MQ=255)

                      aaTACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGt                                                                   <  2:6576813/93‑1 (MQ=255)

                      aaTACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGt                                                                   <  1:5946898/93‑1 (MQ=255)

                      aaTACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGt                                                                   <  2:3365804/93‑1 (MQ=255)

                        tACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTgg                                                                 <  1:1009183/93‑1 (MQ=255)

                         acacTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGt                                                                >  2:3868502/1‑93 (MQ=255)

                           acTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGttt                                                              >  1:1976958/1‑93 (MQ=255)

                            ctctGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTc                                                             >  1:6604941/1‑93 (MQ=255)

                            ctctGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTc                                                             <  2:676228/93‑1 (MQ=255)

                            ctctGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTc                                                             >  1:5152035/1‑93 (MQ=255)

                             tctGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGGTAAGGGCTTCAAGGATAGGAGTCTTCAGTCCCAGATCTGCAAAAGTGGGTTCg                                                            >  1:3793418/1‑93 (MQ=255)

                              ctGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGa                                                           >  2:5442289/1‑93 (MQ=255)

                               tGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGaa                                                          <  2:5580562/93‑1 (MQ=255)

                               tGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGaa                                                          >  2:2984919/1‑93 (MQ=255)

                                gCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAAt                                                         <  1:2156234/93‑1 (MQ=255)

                                gCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAAt                                                         <  2:2762943/93‑1 (MQ=255)

                                gCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAAt                                                         >  2:6444344/1‑93 (MQ=255)

                                  cTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTc                                                       >  1:3927786/1‑93 (MQ=255)

                                  cTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTc                                                       >  2:4402218/1‑93 (MQ=255)

                                   tGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCa                                                      >  1:3720724/1‑93 (MQ=255)

                                   tGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCa                                                      <  1:4866423/93‑1 (MQ=255)

                                     aaTTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGc                                                    <  2:1855989/93‑1 (MQ=255)

                                      aTTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGcc                                                   <  1:109546/93‑1 (MQ=255)

                                       ttGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCa                                                  >  1:3588659/1‑93 (MQ=255)

                                        tGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCAt                                                 >  1:2855303/1‑93 (MQ=255)

                                         ggAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATg                                                >  2:4777155/1‑93 (MQ=255)

                                          gagaTGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgt                                               >  2:1326704/1‑93 (MQ=255)

                                          gagaTGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgt                                               <  1:4922503/93‑1 (MQ=255)

                                           agaTGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgta                                              >  2:3141725/1‑93 (MQ=255)

                                           agaTGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgta                                              >  2:2576074/1‑93 (MQ=255)

                                            gaTGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgtag                                             <  2:6026283/93‑1 (MQ=255)

                                            gaTGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgtag                                             >  1:5391145/1‑93 (MQ=255)

                                            gaTGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgtag                                             >  1:2328175/1‑93 (MQ=255)

                                             attgTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgtagt                                            <  1:4561093/90‑1 (MQ=255)

                                             aTGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgtagt                                            <  1:4350006/93‑1 (MQ=255)

                                             aTGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgtagt                                            >  1:6506949/1‑93 (MQ=255)

                                              tGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgtagta                                           >  2:303147/1‑93 (MQ=255)

                                              tGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATgtagta                                           >  1:5233913/1‑93 (MQ=255)

                                               ggTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTAc                                          <  2:2893166/93‑1 (MQ=255)

                                                 tttttCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACgt                                        <  1:1812589/93‑1 (MQ=255)

                                                 tttttCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACgt                                        <  2:4121878/93‑1 (MQ=255)

                                                  ttttCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACgtg                                       <  1:2767483/93‑1 (MQ=255)

                                                    ttCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGtgtg                                     >  2:516267/1‑93 (MQ=255)

                                                     tCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGc                                    >  1:3898035/1‑93 (MQ=255)

                                                      cGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTAAGCCATGTAGTACGTGTGcc                                   <  2:5559096/93‑1 (MQ=255)

                                                             cAGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAAt                            >  2:3738579/1‑93 (MQ=255)

                                                              aGATCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAAtt                           >  2:3522693/1‑93 (MQ=255)

                                                                 tCGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAAt                        >  1:6241071/1‑93 (MQ=255)

                                                                  cGTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATg                       <  1:1231224/93‑1 (MQ=255)

                                                                   gTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATgg                      >  1:3068617/1‑93 (MQ=255)

                                                                   gTTAAGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATgg                      >  1:2467929/1‑93 (MQ=255)

                                                                    gtAAGGGCTTCAAGGAAAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATggc                     <  1:6093855/92‑1 (MQ=255)

                                                                      aaGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATggcgg                   <  2:5854108/93‑1 (MQ=255)

                                                                       aGGGCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATggcggc                  <  2:2404985/93‑1 (MQ=255)

                                                                        gggCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGcc                 <  1:2234303/93‑1 (MQ=255)

                                                                        gggCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGcc                 <  2:6213038/93‑1 (MQ=255)

                                                                          gCTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAg               <  2:5717023/93‑1 (MQ=255)

                                                                           cTTCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt              >  2:5936283/1‑93 (MQ=255)

                                                                            ttCAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTc             <  2:1644531/93‑1 (MQ=255)

                                                                              cAAGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTa           >  2:1011120/1‑93 (MQ=255)

                                                                               aaGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTAc          <  2:884714/93‑1 (MQ=255)

                                                                               aaGGATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTAc          <  2:1054901/93‑1 (MQ=255)

                                                                                 ggATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACAt        >  2:1709980/1‑93 (MQ=255)

                                                                                 ggATAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACAt        <  1:1615754/93‑1 (MQ=255)

                                                                                   aTAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATaa      <  2:2236833/93‑1 (MQ=255)

                                                                                   aTAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATaa      <  2:1151146/93‑1 (MQ=255)

                                                                                    tAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAAc     >  2:5066653/1‑93 (MQ=255)

                                                                                    tAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAAc     <  2:4331503/93‑1 (MQ=255)

                                                                                    tAGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAAc     <  2:3690877/93‑1 (MQ=255)

                                                                                     aGGAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACt    >  1:5317666/1‑93 (MQ=255)

                                                                                      ggAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACtc   <  1:3191262/93‑1 (MQ=255)

                                                                                      ggAGTCTTCAGGCCCAGATCTGCAAAAGTGGTATCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACtc   >  2:3661936/1‑93 (MQ=255)

                                                                                       gAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACtca  >  2:2375090/1‑93 (MQ=255)

                                                                                       gAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACtca  >  2:1400504/1‑93 (MQ=255)

                                                                                       gAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACtca  >  1:3407216/1‑93 (MQ=255)

                                                                                       gAGTCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACtca  >  1:1284420/1‑93 (MQ=255)

CGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCA  >  NC_000913/3305369‑3305548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`