BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	419,492	T→C	R226R (CGT→CGC)	brnQ →	branched chain amino acid transport system II carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	419,492	0	T	C	100.0%	717.6 / NA	218	R226R (CGT→CGC)	brnQ	branched chain amino acid transport system II carrier protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (115/103); total (115/103)

Only 100 of 225 total aligned reads displayed.

CATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCT  >  NC_000913/419402‑419581

cATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCta                                                                                         <  1:3152781/93‑1 (MQ=255)

cATGGATACGCTGGGCGCAATGGGGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCta                                                                                         >  1:1745410/1‑93 (MQ=255)

 aTGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCtat                                                                                        <  2:5054154/93‑1 (MQ=255)

   ggATACGCTGGGCGCATTGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATAc                                                                                      >  1:6237865/1‑93 (MQ=255)

   ggATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATAc                                                                                      <  1:51639/93‑1 (MQ=255)

    gATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATAcc                                                                                     >  1:1039707/1‑93 (MQ=255)

    gATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATAcc                                                                                     <  1:5584945/93‑1 (MQ=255)

    gATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATAcc                                                                                     >  1:2946801/1‑93 (MQ=255)

      tACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTGCCGAAGCGCGTCTGCTGACCCGCTATACCGt                                                                                   <  2:4924243/93‑1 (MQ=255)

       aCGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTc                                                                                  <  1:4309948/93‑1 (MQ=255)

       aCGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTc                                                                                  <  2:5110890/93‑1 (MQ=255)

       aCGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTc                                                                                  <  2:535387/93‑1 (MQ=255)

          cTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTgg                                                                               >  1:957200/1‑93 (MQ=255)

          cTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTgg                                                                               >  1:6642418/1‑93 (MQ=255)

           tGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTggg                                                                              >  1:4346613/1‑93 (MQ=255)

            gggCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGc                                                                             <  2:1888652/93‑1 (MQ=255)

            gggCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGc                                                                             >  2:3819728/1‑93 (MQ=255)

            gggCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGc                                                                             <  1:5901367/93‑1 (MQ=255)

               cgcAATTGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTgg                                                                          <  1:5548348/93‑1 (MQ=255)

                gcAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGc                                                                         >  2:2946818/1‑93 (MQ=255)

                gcAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGc                                                                         >  2:5414087/1‑93 (MQ=255)

                 cAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGcc                                                                        <  2:247260/93‑1 (MQ=255)

                 cAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGcc                                                                        <  2:2148219/93‑1 (MQ=255)

                  aaTGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCt                                                                       >  2:6177657/1‑93 (MQ=255)

                   aTGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTg                                                                      >  1:2196492/1‑93 (MQ=255)

                    tGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGa                                                                     >  2:2829304/1‑93 (MQ=255)

                    tGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGa                                                                     <  2:5178987/93‑1 (MQ=255)

                    tGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGa                                                                     <  1:6300403/93‑1 (MQ=255)

                    tGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGa                                                                     <  1:3662785/93‑1 (MQ=255)

                    tGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGa                                                                     >  1:5424544/1‑93 (MQ=255)

                     ggTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGAt                                                                    <  2:3279552/93‑1 (MQ=255)

                      gtgtTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATg                                                                   >  2:3642767/1‑93 (MQ=255)

                          ttGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCgg                                                               <  2:5378489/93‑1 (MQ=255)

                           tGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCggg                                                              >  1:3010027/1‑93 (MQ=255)

                            ggTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGgt                                                             <  2:764680/93‑1 (MQ=255)

                               aTCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGtt                                                          >  1:3752842/1‑93 (MQ=255)

                                tCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTg                                                         <  1:3206293/93‑1 (MQ=255)

                                 cGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTgg                                                        <  2:5804861/93‑1 (MQ=255)

                                   ttATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTc                                                      <  2:4777322/93‑1 (MQ=255)

                                   ttATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTc                                                      >  1:3071235/1‑93 (MQ=255)

                                    tATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCt                                                     >  1:5623082/1‑93 (MQ=255)

                                    tATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCt                                                     <  2:3477513/93‑1 (MQ=255)

                                     aTTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGGGTTGGTCTg                                                    >  2:6497921/1‑93 (MQ=255)

                                      ttGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGa                                                   >  2:5545510/1‑93 (MQ=255)

                                       tGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGAc                                                  >  1:1588539/1‑93 (MQ=255)

                                       tGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGAc                                                  >  2:1820248/1‑93 (MQ=255)

                                       tGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGGGTTGGTATGAc                                                  >  2:4412678/1‑93 (MQ=255)

                                        gTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGAct                                                 >  2:3861529/1‑93 (MQ=255)

                                        gTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGAct                                                 >  1:1174421/1‑93 (MQ=255)

                                        gTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGGCTGAct                                                 >  2:2276465/1‑93 (MQ=255)

                                        gTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGGTGGTCTGAct                                                 >  2:4750062/1‑93 (MQ=255)

                                         ttAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGActc                                                <  2:5959457/93‑1 (MQ=255)

                                          tAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGActct                                               <  2:4698787/93‑1 (MQ=255)

                                              gcggcgCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTctgctg                                           >  2:1689960/1‑93 (MQ=255)

                                              gcggcgCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTctgctg                                           <  1:4504747/93‑1 (MQ=255)

                                              gcggcgCGTTCTCGTGGCGTTACCGAAGCGCGGCTGCTGACCCGCTATACCGTCTGGGCTGGGCCGATGGCGGGGGGTGGTCTGACTctgctg                                           >  1:3573318/1‑93 (MQ=255)

                                               cggcgCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGt                                          <  1:2053880/93‑1 (MQ=255)

                                                ggcgCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTa                                         >  2:1742726/1‑93 (MQ=255)

                                                  cgcgTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTAcc                                       <  1:5317514/93‑1 (MQ=255)

                                                  cgcgTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTAcc                                       <  2:5288338/93‑1 (MQ=255)

                                                  cgcgTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTAcc                                       <  2:6101196/93‑1 (MQ=255)

                                                  cgcgTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTAcc                                       >  1:51085/1‑93 (MQ=255)

                                                  cgcgTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTAcc                                       <  2:2643675/93‑1 (MQ=255)

                                                      ttCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGgc                                   >  2:5238957/1‑93 (MQ=255)

                                                      ttCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGgc                                   >  1:1296678/1‑93 (MQ=255)

                                                       tctcGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGgcg                                  >  2:6628038/1‑93 (MQ=255)

                                                       tctcGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGgcg                                  >  2:4100297/1‑93 (MQ=255)

                                                       tctcGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGCGTTTGTCTGACTCTGCTGTACCTGgcg                                  >  1:2155073/1‑93 (MQ=255)

                                                        ctcGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGgcgc                                 <  2:278214/93‑1 (MQ=255)

                                                         tcGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCt                                <  1:6472935/93‑1 (MQ=255)

                                                         tcGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCt                                <  1:5862976/93‑1 (MQ=255)

                                                         tcGTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGCGTTGGTCTGACTCTGCTGTACCTGGCGCt                                >  2:3355998/1‑93 (MQ=255)

                                                           gTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGt                              >  2:140540/1‑93 (MQ=255)

                                                           gTGGCGTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCACTGt                              <  2:6671830/93‑1 (MQ=255)

                                                                gTTACCGACGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGt                         >  1:6514767/1‑93 (MQ=255)

                                                                gTTACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGAGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGt                         >  2:2584105/1‑93 (MQ=255)

                                                                 ttACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTc                        <  2:379655/93‑1 (MQ=255)

                                                                 ttACCGAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTc                        <  1:4287924/93‑1 (MQ=255)

                                                                    ccGAACCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTgg                     <  1:4613977/93‑1 (MQ=255)

                                                                      gAAGCGCGTCTGCTGACCCGCTATGCCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTgggg                   <  2:1201009/93‑1 (MQ=255)

                                                                      gAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTgggg                   <  1:4868242/93‑1 (MQ=255)

                                                                      gAAGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTgggg                   <  2:2886434/93‑1 (MQ=255)

                                                                       aaGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGAGTTGGTCTGACTCTGCTGTACCTCGCGCTGTTCCGTCTGGGGt                  >  2:2918746/1‑93 (MQ=255)

                                                                        aGCGCGTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTc                 <  2:5429600/93‑1 (MQ=255)

                                                                           gcgTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGa              >  2:1714893/1‑93 (MQ=255)

                                                                           gcgTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGGCAGa              >  1:5928375/1‑93 (MQ=255)

                                                                            cgTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGAc             >  2:4317548/1‑93 (MQ=255)

                                                                            cgTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGAc             >  1:82646/1‑93 (MQ=255)

                                                                             gTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACa            >  1:2678362/1‑93 (MQ=255)

                                                                             gTCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACa            >  1:468281/1‑93 (MQ=255)

                                                                              tCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAg           <  2:4246655/93‑1 (MQ=255)

                                                                              tCTGCTGACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAg           <  2:4205097/93‑1 (MQ=255)

                                                                                tgctgACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACGgcg         >  2:5479858/1‑93 (MQ=255)

                                                                                tgctgACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAgcg         <  2:4962235/93‑1 (MQ=255)

                                                                                  ctgACCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGcgcg       <  1:272819/93‑1 (MQ=255)

                                                                                     aCCCGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCg    <  1:3539047/93‑1 (MQ=255)

                                                                                      cccGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGc   <  1:4141390/93‑1 (MQ=255)

                                                                                      cccGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGc   <  1:24747/93‑1 (MQ=255)

                                                                                      cccGCTATACCGTCTGGGCTGGCCCGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTTGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGc   >  1:3890466/1‑93 (MQ=255)

                                                                                       ccGCTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCt  >  1:5956579/1‑93 (MQ=255)

CATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCT  >  NC_000913/419402‑419581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`