BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,407,232	G→A	A176T (GCG→ACG)	prmA →	PrmA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,407,232	0	G	A	99.6%	861.4 / ‑5.4	233	A176T (GCG→ACG)	prmA	PrmA
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (98/134); total (99/134)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.14e-01

Only 100 of 248 total aligned reads displayed.

TCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGC  >  NC_000913/3407142‑3407321

  tctGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCt                                                                                       <  1:551672/93‑1 (MQ=255)

  tctGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCt                                                                                       <  2:6234181/93‑1 (MQ=255)

   ctGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTg                                                                                      >  1:6290208/1‑93 (MQ=255)

    tgtgCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGa                                                                                     >  1:1232093/1‑93 (MQ=255)

    tgtgCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGa                                                                                     <  2:3509414/93‑1 (MQ=255)

     gtgCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGaa                                                                                    <  1:4356854/93‑1 (MQ=255)

     gtgCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGaa                                                                                    >  2:5850417/1‑93 (MQ=255)

      tgCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGaaa                                                                                   <  2:2915778/93‑1 (MQ=255)

      tgCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGaaa                                                                                   <  1:860547/93‑1 (MQ=255)

       gCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAAc                                                                                  <  1:1596768/93‑1 (MQ=255)

        ccTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACt                                                                                 <  2:1651962/93‑1 (MQ=255)

          tGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTgg                                                                               >  2:333266/1‑93 (MQ=255)

           gCAATGGCTCGACAGCCTCGATTTAGCCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTggg                                                                              <  1:3405473/93‑1 (MQ=255)

           gCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTggg                                                                              >  2:6413996/1‑93 (MQ=255)

           gCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTggg                                                                              <  1:3976554/93‑1 (MQ=255)

            cAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGt                                                                             >  1:563800/1‑93 (MQ=255)

              aTGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTgc                                                                           >  1:6599253/1‑93 (MQ=255)

              aTGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTgc                                                                           <  2:2998075/93‑1 (MQ=255)

                ggCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTgcag                                                                         >  1:1721948/1‑93 (MQ=255)

                ggCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTgcac                                                                         >  1:3077046/1‑92 (MQ=255)

                     gACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCaaaa                                                                    <  1:4225285/93‑1 (MQ=255)

                     gACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCaaaa                                                                    <  2:4536810/93‑1 (MQ=255)

                     gACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCaaaa                                                                    <  1:5137907/93‑1 (MQ=255)

                       cAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGc                                                                  <  2:5988157/93‑1 (MQ=255)

                       cAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGc                                                                  >  2:2476347/1‑93 (MQ=255)

                         gCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCa                                                                <  1:6468745/93‑1 (MQ=255)

                         gCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCa                                                                <  1:5263696/93‑1 (MQ=255)

                          ccTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCAt                                                               >  2:797909/1‑93 (MQ=255)

                           cTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCAtt                                                              <  2:6616948/93‑1 (MQ=255)

                           cTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCAtt                                                              <  1:5977386/93‑1 (MQ=255)

                            tCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTg                                                             >  2:2646707/1‑93 (MQ=255)

                            tCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTg                                                             >  1:359591/1‑93 (MQ=255)

                            tCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTg                                                             <  1:783794/93‑1 (MQ=255)

                            tCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTg                                                             >  2:1002541/1‑93 (MQ=255)

                             cGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTgg                                                            <  2:399721/93‑1 (MQ=255)

                              gATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGt                                                           <  1:6068640/93‑1 (MQ=255)

                                tttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAt                                                         <  1:5079292/93‑1 (MQ=255)

                                tttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAt                                                         >  2:4570032/1‑93 (MQ=255)

                                tttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAt                                                         >  1:932697/1‑93 (MQ=255)

                                tttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAt                                                         >  1:206768/1‑93 (MQ=255)

                                tttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAt                                                         <  1:2929424/93‑1 (MQ=255)

                                 ttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAtt                                                        <  2:5050625/93‑1 (MQ=255)

                                   aaCCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGa                                                      >  2:6019127/1‑93 (MQ=255)

                                    aCCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGat                                                     >  2:6102798/1‑93 (MQ=255)

                                     ccGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGata                                                    >  1:47674/1‑93 (MQ=255)

                                     ccGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGata                                                    <  1:5761744/93‑1 (MQ=255)

                                      cGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGatat                                                   >  2:5612537/1‑93 (MQ=255)

                                      cGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGatat                                                   <  2:1360454/93‑1 (MQ=255)

                                      cGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGatat                                                   >  1:261932/1‑93 (MQ=255)

                                       ggTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATc                                                  <  2:3052023/93‑1 (MQ=255)

                                          aaaaCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGAt                                               <  2:2869713/93‑1 (MQ=255)

                                           aaaCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATc                                              <  2:2209750/93‑1 (MQ=255)

                                             aCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCg                                            >  2:84949/1‑93 (MQ=255)

                                              cAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGc                                           >  1:3731673/1‑93 (MQ=255)

                                                gTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAg                                         >  2:527445/1‑93 (MQ=255)

                                                 tCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAGCTGGGTGCAGCAAAAGGCATTGGTATTGATATCGATCCGCAgg                                        >  1:4539902/1‑93 (MQ=255)

                                                    tCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa                                     >  2:926611/1‑93 (MQ=255)

                                                      gACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGAtt                                   <  2:3573231/93‑1 (MQ=255)

                                                       aCTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTc                                  >  2:6550679/1‑93 (MQ=255)

                                                       aCTTTGGCTGTGGTTCAGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTc                                  >  1:3112204/1‑93 (MQ=255)

                                                        cTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 <  2:2548235/93‑1 (MQ=255)

                                                        cTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 <  1:4934963/93‑1 (MQ=255)

                                                        cTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 <  1:2799420/93‑1 (MQ=255)

                                                        cTTTGGCTGTGGTTCCGCCATTCTGGCGATCGCGATGCTGAAACTGGGCGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 <  1:6518532/93‑1 (MQ=255)

                                                         tttGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAg                                >  1:4409859/1‑93 (MQ=255)

                                                         tttGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAg                                >  2:3435545/1‑93 (MQ=255)

                                                           tGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGc                              <  2:1928112/93‑1 (MQ=255)

                                                            ggCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGcc                             <  2:3164290/93‑1 (MQ=255)

                                                            ggCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGcc                             <  2:3782776/93‑1 (MQ=255)

                                                             gCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCa                            <  2:5894930/93‑1 (MQ=255)

                                                             gCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCa                            <  1:4010125/93‑1 (MQ=255)

                                                               tgtgGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGc                          <  2:698960/93‑1 (MQ=255)

                                                               tgtgGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGc                          <  2:1963890/93‑1 (MQ=255)

                                                               tgtgGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGc                          >  2:4115442/1‑93 (MQ=255)

                                                               tgtgGTTCCGGCATTCCGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGc                          <  1:2993797/93‑1 (MQ=255)

                                                                 tgGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCcg                        <  1:1025862/93‑1 (MQ=255)

                                                                 gggTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCcg                        <  1:4697449/92‑1 (MQ=255)

                                                                  ggTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCcgc                       <  2:5449465/93‑1 (MQ=255)

                                                                   gTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCcgcg                      >  2:4825566/1‑93 (MQ=255)

                                                                    ttCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGa                     <  1:1784192/93‑1 (MQ=255)

                                                                       cGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa                  <  1:5745751/93‑1 (MQ=255)

                                                                        ggCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAATCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAAc                 <  2:5293034/93‑1 (MQ=255)

                                                                         gCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACg                >  2:6441001/1‑93 (MQ=255)

                                                                         gCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACg                >  1:4329338/1‑93 (MQ=255)

                                                                          cATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAGTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGc               <  2:4690801/93‑1 (MQ=255)

                                                                           aTTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGcg              >  1:4828722/1‑92 (MQ=255)

                                                                           aTTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGcc              <  2:604229/93‑1 (MQ=255)

                                                                           aTTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGcc              >  1:1996669/1‑93 (MQ=255)

                                                                            ttCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg             >  1:2567290/1‑93 (MQ=255)

                                                                            ttCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg             >  2:1660319/1‑93 (MQ=255)

                                                                              cTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGaa           <  1:3285091/93‑1 (MQ=255)

                                                                              cTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGaa           <  2:1433304/93‑1 (MQ=255)

                                                                               tGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAAc          <  2:6220246/93‑1 (MQ=255)

                                                                               tGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAAc          >  2:1242060/1‑93 (MQ=255)

                                                                               tGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAAc          <  2:5101889/93‑1 (MQ=255)

                                                                                   gATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTaa      <  1:641150/93‑1 (MQ=255)

                                                                                   gATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTaa      <  1:2286143/93‑1 (MQ=255)

                                                                                   gATCGAGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTaa      <  1:4593694/93‑1 (MQ=255)

                                                                                    aTCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAAt     >  1:6655850/1‑93 (MQ=255)

                                                                                       gcgacgCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGc  <  2:4670796/93‑1 (MQ=255)

TCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGC  >  NC_000913/3407142‑3407321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`