BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,427,075	T→C	V68A (GTC→GCC)	b3279 →	putative transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,427,075	0	T	C	99.7%	963.4 / NA	299	V68A (GTC→GCC)	b3279	putative transferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (141/157); minor base G (1/0); total (142/157)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 301 total aligned reads displayed.

GGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCT  >  NC_000913/3426985‑3427164

  aTCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACt                                                                                       <  1:2984751/93‑1 (MQ=255)

   tCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTc                                                                                      <  1:4678204/93‑1 (MQ=255)

    cTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCa                                                                                     >  2:4702909/1‑93 (MQ=255)

     tGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCAt                                                                                    >  2:1202592/1‑93 (MQ=255)

     tGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCAt                                                                                    >  2:3103392/1‑93 (MQ=255)

      ggCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATa                                                                                   <  2:578626/93‑1 (MQ=255)

      ggCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATa                                                                                   >  2:3514887/1‑93 (MQ=255)

       gCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATaa                                                                                  <  2:1028123/93‑1 (MQ=255)

       gCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATaa                                                                                  <  1:3942637/93‑1 (MQ=255)

        ccGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATaaa                                                                                 <  2:868720/93‑1 (MQ=255)

        ccGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATaaa                                                                                 <  1:3299817/93‑1 (MQ=255)

          gCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATc                                                                               <  2:4495984/93‑1 (MQ=255)

          gCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATc                                                                               >  1:412389/1‑93 (MQ=255)

          gCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATc                                                                               >  2:4569010/1‑93 (MQ=255)

            tCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCt                                                                             <  1:2076174/93‑1 (MQ=255)

            tCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCt                                                                             >  2:4370408/1‑93 (MQ=255)

             cGTTGTGATTCGTGGAGATGTACATTATGTAGAGATCGGAGCACTCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTc                                                                            <  1:5970682/93‑1 (MQ=255)

             cGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTc                                                                            <  2:6238843/93‑1 (MQ=255)

              gTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCg                                                                           >  1:445893/1‑93 (MQ=255)

              gTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCg                                                                           >  2:6527943/1‑93 (MQ=255)

                tgtgATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATTCCACTCATAAATCCTCGTa                                                                         <  1:6530368/93‑1 (MQ=255)

                   gATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACaa                                                                      <  1:2167377/93‑1 (MQ=255)

                    aTTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAAc                                                                     <  1:4992826/93‑1 (MQ=255)

                     ttCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAAcc                                                                    >  1:3854264/1‑93 (MQ=255)

                     ttCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAAcc                                                                    >  1:2680186/1‑93 (MQ=255)

                     ttCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAAcc                                                                    >  2:4153546/1‑93 (MQ=255)

                      tCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAAccc                                                                   <  2:217940/93‑1 (MQ=255)

                      tCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAAccc                                                                   <  1:1102512/93‑1 (MQ=255)

                          ggAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGAt                                                               <  2:651391/93‑1 (MQ=255)

                          ggAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGAt                                                               >  1:5177011/1‑93 (MQ=255)

                          ggAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGAt                                                               >  1:4606796/1‑93 (MQ=255)

                           gagaTGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATg                                                              <  1:2925231/93‑1 (MQ=255)

                           gagaTGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATg                                                              <  2:3879350/93‑1 (MQ=255)

                               tGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCaa                                                          >  1:3353879/1‑93 (MQ=255)

                                 tACATTGTGGACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGTGGGCAAcc                                                        >  1:1477198/1‑93 (MQ=255)

                                  aCATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAAccc                                                       <  2:1106762/93‑1 (MQ=255)

                                    aTTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAt                                                     <  1:278284/93‑1 (MQ=255)

                                     ttATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAtt                                                    >  1:307239/1‑93 (MQ=255)

                                     ttATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAtt                                                    <  1:5537771/93‑1 (MQ=255)

                                        tGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTacc                                                 >  2:3358089/1‑91 (MQ=255)

                                        tGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAc                                                 >  2:4915694/1‑93 (MQ=255)

                                         gTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAcc                                                <  2:431294/93‑1 (MQ=255)

                                         gTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAcc                                                >  2:1809550/1‑93 (MQ=255)

                                         gTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAcc                                                >  1:1173297/1‑93 (MQ=255)

                                          tACAGCTCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCCTACAACCCAGATGGCAACCCAGTAGCCa                                               <  1:1824818/93‑1 (MQ=255)

                                          tACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCa                                               >  2:1692385/1‑93 (MQ=255)

                                           aCAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAt                                              >  2:6690006/1‑93 (MQ=255)

                                            cAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAtt                                             <  2:592878/93‑1 (MQ=255)

                                             aGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTg                                            <  2:5587008/93‑1 (MQ=255)

                                                tCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCg                                         >  1:3460771/1‑93 (MQ=255)

                                                 cGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGa                                        >  1:1903726/1‑93 (MQ=255)

                                                 cGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGa                                        >  1:943917/1‑93 (MQ=255)

                                                  ggAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCTACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGaa                                       <  2:5430665/93‑1 (MQ=255)

                                                  ggAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGaa                                       >  1:4859175/1‑93 (MQ=255)

                                                   gAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAg                                      >  1:6393511/1‑93 (MQ=255)

                                                   gAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAg                                      >  2:3625382/1‑93 (MQ=255)

                                                    aGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGa                                     <  2:1491557/93‑1 (MQ=255)

                                                    aGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATAGGCGAAGa                                     <  1:6566294/93‑1 (MQ=255)

                                                     gcacgcacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAt                                    <  1:2859924/93‑1 (MQ=255)

                                                     gcacgcacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAt                                    >  1:5253090/1‑93 (MQ=255)

                                                     gcacgcacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAt                                    <  2:2662963/93‑1 (MQ=255)

                                                      cacgcacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATg                                   >  2:2019511/1‑93 (MQ=255)

                                                          ctccAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAc                               <  2:3820043/91‑1 (MQ=255)

                                                          cacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAc                               <  2:1924133/93‑1 (MQ=255)

                                                          cacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAc                               <  2:3540140/93‑1 (MQ=255)

                                                          cacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAc                               <  1:4237585/93‑1 (MQ=255)

                                                           acCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACt                              <  1:144162/93‑1 (MQ=255)

                                                              aaTATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGtt                           <  2:2486985/93‑1 (MQ=255)

                                                              aaTATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGtt                           <  1:6075189/93‑1 (MQ=255)

                                                              aaTATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGtt                           >  1:5649957/1‑93 (MQ=255)

                                                               atatCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTg                          <  2:5078415/93‑1 (MQ=255)

                                                               atatCCAGGATCGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTg                          <  1:4525173/93‑1 (MQ=255)

                                                                tatCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTgg                         <  1:5314653/93‑1 (MQ=255)

                                                                 atCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGt                        >  2:5035821/1‑93 (MQ=255)

                                                                    cAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTcac                     >  1:1663374/1‑93 (MQ=255)

                                                                    cAGGATGGCAGTATGTTGCATGCCACTCATAAATACTCGTACAACCCAGATGGCAACGCATTAACCATTGGCGAAGGTGTCACTGTTGGTcag                     >  1:4323984/1‑92 (MQ=255)

                                                                       gATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAg                  <  1:1732015/93‑1 (MQ=255)

                                                                       gATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAg                  >  1:197752/1‑93 (MQ=255)

                                                                        aTGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAgg                 >  2:6489405/1‑93 (MQ=255)

                                                                        aTGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAgg                 <  1:4774461/93‑1 (MQ=255)

                                                                        aTGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAgg                 <  1:6109697/93‑1 (MQ=255)

                                                                         tGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGt                <  2:5237956/93‑1 (MQ=255)

                                                                         tGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGt                <  1:1044384/93‑1 (MQ=255)

                                                                           gCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGa              >  1:2281574/1‑93 (MQ=255)

                                                                           gCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGa              <  1:5841050/93‑1 (MQ=255)

                                                                           gCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGa              <  1:2457736/93‑1 (MQ=255)

                                                                            cAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAt             <  1:803401/93‑1 (MQ=255)

                                                                              gTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGc           >  1:372673/1‑93 (MQ=255)

                                                                              gTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGc           >  2:4520312/1‑93 (MQ=255)

                                                                              gTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGc           >  1:5460875/1‑93 (MQ=255)

                                                                                 tGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACACGGTGATGCTcc        >  2:1365763/1‑93 (MQ=255)

                                                                                 tGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTcc        >  1:1196217/1‑93 (MQ=255)

                                                                                  gTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCa       >  1:2396097/1‑93 (MQ=255)

                                                                                  gTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCa       <  1:2819949/93‑1 (MQ=255)

                                                                                   ttGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCAc      <  2:2850700/93‑1 (MQ=255)

                                                                                   ttGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCAc      <  2:3197864/93‑1 (MQ=255)

                                                                                    tGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACg     >  2:6087535/1‑93 (MQ=255)

                                                                                      cATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGc   <  2:5659929/93‑1 (MQ=255)

                                                                                      cATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGc   <  2:2624574/93‑1 (MQ=255)

                                                                                       aTGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCt  >  1:3197518/1‑93 (MQ=255)

GGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCT  >  NC_000913/3426985‑3427164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`