BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,463,677	C→T	G166G (GGC→GGT)	hofD →	leader peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,463,677	0	C	T	99.1%	815.3 / NA	226	G166G (GGC→GGT)	hofD	leader peptidase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (104/120); minor base G (1/1); total (105/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 229 total aligned reads displayed.

GGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGTTTCACAACTTCTGCA  >  NC_000913/3463587‑3463766

 gTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCa                                                                                        <  1:6072991/93‑1 (MQ=255)

 gTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCa                                                                                        >  1:946065/1‑93 (MQ=255)

 gTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCa                                                                                        <  2:6017310/93‑1 (MQ=255)

 gTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCa                                                                                        <  2:4068350/93‑1 (MQ=255)

  tGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCAt                                                                                       <  1:5703393/93‑1 (MQ=255)

     tgtgGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATggg                                                                                    >  2:1550880/1‑92 (MQ=255)

     tgtgGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGgt                                                                                    <  1:1134931/93‑1 (MQ=255)

     tgtgGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGgt                                                                                    <  2:1859289/93‑1 (MQ=255)

      gtggtgTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGgtg                                                                                   >  2:693175/1‑93 (MQ=255)

        ggtgTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGtgtg                                                                                 >  1:3645788/1‑93 (MQ=255)

         gtgtgtTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGtgg                                                                                >  1:2514036/1‑93 (MQ=255)

          tgtgtTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGtggc                                                                               <  2:3563159/93‑1 (MQ=255)

          tgtgtTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGtggc                                                                               <  1:6298118/93‑1 (MQ=255)

            tgtTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTTGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGtggctg                                                                             >  1:6211053/1‑93 (MQ=255)

            tgtTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAATCTACTGGCCGCTGCAGGTGCATGGTGtggctg                                                                             >  1:4218084/1‑93 (MQ=255)

            tgtTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGtggctg                                                                             >  2:5520472/1‑93 (MQ=255)

            tgtTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGtggctg                                                                             >  2:6600785/1‑93 (MQ=255)

               ttACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCa                                                                          <  1:6180034/93‑1 (MQ=255)

                tACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCAAGGTGtggctggcca                                                                         >  2:5004089/1‑91 (MQ=255)

                 aCTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCaaa                                                                        <  1:5230613/93‑1 (MQ=255)

                 aCTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCaaa                                                                        <  2:3123285/93‑1 (MQ=255)

                  cTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAAc                                                                       <  1:2380535/93‑1 (MQ=255)

                    ggggCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGt                                                                     <  2:1963206/93‑1 (MQ=255)

                    ggggCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGt                                                                     <  2:3233721/93‑1 (MQ=255)

                       gCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGGAAACGTTGc                                                                  >  1:5150206/1‑93 (MQ=255)

                         gTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCa                                                                >  2:1274663/1‑93 (MQ=255)

                          tCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCaa                                                               <  2:6359886/93‑1 (MQ=255)

                          tCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCaa                                                               <  1:6461759/93‑1 (MQ=255)

                           cTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAAt                                                              <  1:1516704/93‑1 (MQ=255)

                            tGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATg                                                             >  2:6466134/1‑93 (MQ=255)

                              gTTCGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGAt                                                           <  2:2369505/93‑1 (MQ=255)

                              gTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGAt                                                           <  1:34522/93‑1 (MQ=255)

                               ttAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATa                                                          <  2:474930/93‑1 (MQ=255)

                               ttAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATa                                                          >  2:5170868/1‑93 (MQ=255)

                               ttAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGGTGCCAATGATa                                                          >  1:4151157/1‑93 (MQ=255)

                                tAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATAc                                                         <  2:1095529/93‑1 (MQ=255)

                                  gTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATActg                                                       >  1:3190355/1‑93 (MQ=255)

                                   tttGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATActgc                                                      >  2:1615202/1‑93 (MQ=255)

                                   tttGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATActgc                                                      <  2:4802930/93‑1 (MQ=255)

                                    ttGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGGTGCCAATGATActgct                                                     >  2:4485244/1‑93 (MQ=255)

                                      gTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGa                                                   <  2:1601240/93‑1 (MQ=255)

                                       tCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGAt                                                  <  1:4071008/93‑1 (MQ=255)

                                       tCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGAt                                                  <  2:1767432/93‑1 (MQ=255)

                                       tCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGAt                                                  <  2:4540841/93‑1 (MQ=255)

                                       tCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGAt                                                  >  2:4549057/1‑93 (MQ=255)

                                         acaAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGGCAATGATACTGCTGATTg                                                >  1:6390758/1‑93 (MQ=255)

                                         acaAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTTATTg                                                >  1:6089073/1‑93 (MQ=255)

                                         acaAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTg                                                >  1:4492708/1‑93 (MQ=255)

                                         acaAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTg                                                >  2:3654255/1‑93 (MQ=255)

                                           aaaGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTTATTGcc                                              >  1:5605526/1‑93 (MQ=255)

                                            aaGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGccc                                             >  2:5464572/1‑92 (MQ=255)

                                            aaGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCt                                             <  2:4724515/93‑1 (MQ=255)

                                            aaGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCt                                             <  1:2235879/93‑1 (MQ=255)

                                            aaGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCt                                             <  2:2575405/93‑1 (MQ=255)

                                            aaGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCt                                             >  2:2447868/1‑93 (MQ=255)

                                            aaGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCt                                             <  2:2130731/93‑1 (MQ=255)

                                            aaGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCt                                             <  2:6670433/93‑1 (MQ=255)

                                            aaGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCt                                             >  2:133521/1‑93 (MQ=255)

                                            aaGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCt                                             <  1:4290603/93‑1 (MQ=255)

                                              gagGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTcc                                           >  2:6088230/1‑92 (MQ=255)

                                              gagGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCg                                           >  1:3772129/1‑93 (MQ=255)

                                                gggATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCt                                         <  1:3578721/93‑1 (MQ=255)

                                                  gATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTgg                                       >  1:5499251/1‑93 (MQ=255)

                                                    ttGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTgggg                                     >  2:3086759/1‑92 (MQ=255)

                                                      gggCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTgg                                   >  1:3742401/1‑93 (MQ=255)

                                                       ggCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGc                                  >  1:6527273/1‑93 (MQ=255)

                                                          tACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCAtt                               <  1:6281888/93‑1 (MQ=255)

                                                          tACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCAtt                               >  2:4862848/1‑93 (MQ=255)

                                                          tACGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCAtt                               >  1:1618813/1‑93 (MQ=255)

                                                            cGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTgg                             >  2:288906/1‑93 (MQ=255)

                                                            cGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTgg                             <  2:2017090/93‑1 (MQ=255)

                                                            cGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTgg                             <  1:443552/93‑1 (MQ=255)

                                                            cGGTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTgg                             <  1:5880650/93‑1 (MQ=255)

                                                             ggTGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGt                            <  1:1063752/93‑1 (MQ=255)

                                                               tGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTTGTTa                          >  1:2656196/1‑93 (MQ=255)

                                                               tGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTa                          >  2:325156/1‑93 (MQ=255)

                                                               tGATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTa                          <  2:2615308/93‑1 (MQ=255)

                                                                gATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTAc                         <  1:2833556/93‑1 (MQ=255)

                                                                gATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTAc                         <  1:2447534/93‑1 (MQ=255)

                                                                gATTTCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTAc                         <  1:4724994/93‑1 (MQ=255)

                                                                  tttCAGGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGc                       <  1:779724/93‑1 (MQ=255)

                                                                  tttCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATCGGTTACGc                       >  1:4788709/1‑93 (MQ=255)

                                                                    tCAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCa                     <  1:5322639/93‑1 (MQ=255)

                                                                     cAAGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGG‑ATTTGTTAAGCCATc                   >  1:6604861/1‑93 (MQ=255)

                                                                      aaGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATc                   >  2:4918858/1‑93 (MQ=255)

                                                                      aaGCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATc                   <  1:3155060/93‑1 (MQ=255)

                                                                        gCTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGt                 <  2:5960375/93‑1 (MQ=255)

                                                                         cTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGtt                <  1:5036848/93‑1 (MQ=255)

                                                                         cTACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGtt                <  2:3423797/93‑1 (MQ=255)

                                                                          tACTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGttt               >  2:2301365/1‑93 (MQ=255)

                                                                           aCTGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATAATGCTGATTGCGTCGCTGGGGGGCATTGGTTACGCCATCGCTTc              >  1:2023770/1‑93 (MQ=255)

                                                                             tGGCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGTTTcac            >  2:2754413/1‑93 (MQ=255)

                                                                              ggCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGTTTcaca           >  2:3438706/1‑93 (MQ=255)

                                                                               gCCGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGTTTCACaa          <  2:4455303/93‑1 (MQ=255)

                                                                                ccGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGGTTGCCTCGCTGGGTGGCATTGGTTACGCCATCGTTTCACAAc         <  1:974283/93‑1 (MQ=255)

                                                                                 cGCTGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGTTTCACAACt        >  2:5790856/1‑93 (MQ=255)

                                                                                    tGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGTTTCACAACTTCt     <  1:4622348/93‑1 (MQ=255)

                                                                                    tGCAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGTTTCACAACTTCt     >  2:767686/1‑93 (MQ=255)

                                                                                      cAGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGTTTCACAACTTCTGc   >  1:18974/1‑93 (MQ=255)

                                                                                       aGGTGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGTTTCACAACTTCTGCa  >  2:5304071/1‑93 (MQ=255)

GGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGGTTACGCCATCGTTTCACAACTTCTGCA  >  NC_000913/3463587‑3463766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`