Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,483,814 C→T H20Y (CAC→TAC)  crp → cyclic AMP receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,483,8140CT99.7% 1099.8 / NA 293H20Y (CAC→TAC) crpcyclic AMP receptor protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (141/151);  minor base G (0/1);  total (141/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

CTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCA  >  NC_000913/3483724‑3483903
                                                                                          |                                                                                         
ctGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCtac                                                                                         <  2:5611360/93‑1 (MQ=255)
  ggAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCTACAt                                                                                       <  1:810838/93‑1 (MQ=255)
  ggAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCTACAt                                                                                       <  1:4547888/93‑1 (MQ=255)
   gagaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACAtt                                                                                      >  2:6054811/1‑93 (MQ=255)
    agaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTc                                                                                     >  1:6328728/1‑93 (MQ=255)
    agaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTc                                                                                     >  1:4370306/1‑93 (MQ=255)
    agaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTc                                                                                     <  1:2574650/93‑1 (MQ=255)
     gaAAGCTTATAACAGAGGATACCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCa                                                                                    <  1:4267415/93‑1 (MQ=255)
         gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGAGCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                <  2:3567741/93‑1 (MQ=255)
         gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                >  1:1011507/1‑93 (MQ=255)
         gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                <  1:5858135/93‑1 (MQ=255)
         gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                >  2:4478663/1‑93 (MQ=255)
          cTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGt                                                                               >  2:3115321/1‑93 (MQ=255)
           ttATAACAGAGGATAACCGCGCATGGCGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTa                                                                              >  2:2828033/1‑93 (MQ=255)
             ataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTAcc                                                                            >  1:96064/1‑93 (MQ=255)
              taACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTAccc                                                                           <  1:1189329/93‑1 (MQ=255)
              taACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTAccc                                                                           >  1:4113266/1‑93 (MQ=255)
               aaCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCa                                                                          <  2:5110483/93‑1 (MQ=255)
                aCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCAt                                                                         >  1:5804609/1‑93 (MQ=255)
                 cggagGATAACCGCGCAT‑GTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATcc                                                                       >  1:5311256/3‑93 (MQ=255)
                 cAGAGGATAACCGCGCATGGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATc                                                                        <  2:1288584/93‑1 (MQ=255)
                  agagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATcc                                                                       <  2:4770738/93‑1 (MQ=255)
                    agGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCaa                                                                     <  2:6240899/93‑1 (MQ=255)
                     ggATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAag                                                                    >  1:1233689/1‑93 (MQ=255)
                       aTAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAagag                                                                  >  2:5380959/1‑93 (MQ=255)
                        tAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGc                                                                 <  1:5724271/93‑1 (MQ=255)
                         aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCa                                                                >  1:1977976/1‑93 (MQ=255)
                            cgcgCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGc                                                             <  1:5024033/93‑1 (MQ=255)
                             gcgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCt                                                            <  2:6114216/93‑1 (MQ=255)
                              cgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCtt                                                           >  2:1375120/1‑93 (MQ=255)
                              cgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCtt                                                           <  1:2765417/93‑1 (MQ=255)
                               gcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTa                                                          >  2:1873013/1‑93 (MQ=255)
                                cATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTAt                                                         >  2:863079/1‑93 (MQ=255)
                                cATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTAt                                                         >  2:5758574/1‑93 (MQ=255)
                                cATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTAt                                                         <  2:2599503/93‑1 (MQ=255)
                                cATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTAt                                                         >  2:3921986/1‑93 (MQ=255)
                                 aTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTAtt                                                        >  1:1389760/1‑93 (MQ=255)
                                 aTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTAtt                                                        <  1:3302950/93‑1 (MQ=255)
                                 aTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTAtt                                                        >  1:5580388/1‑93 (MQ=255)
                                 aTGGTGCTTGCCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTAtt                                                        <  1:1568951/93‑1 (MQ=255)
                                  tGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTc                                                       <  2:684056/93‑1 (MQ=255)
                                    gTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCAc                                                     >  2:6617708/1‑93 (MQ=255)
                                     tGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCAcc                                                    <  2:1786132/93‑1 (MQ=255)
                                      gCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCa                                                   >  2:2138729/1‑93 (MQ=255)
                                        ttGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgg                                                 >  1:5097410/1‑93 (MQ=255)
                                          ggCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgggg                                               >  1:4630152/1‑92 (MQ=255)
                                          ggCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGt                                               <  1:4441190/93‑1 (MQ=255)
                                              aaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                           >  2:3871282/1‑93 (MQ=255)
                                               aCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                          <  2:3997404/93‑1 (MQ=255)
                                               aCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                          <  2:6440346/93‑1 (MQ=255)
                                                ccGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaaa                                         <  2:5659059/93‑1 (MQ=255)
                                                ccGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaaa                                         <  1:108032/93‑1 (MQ=255)
                                                ccGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaaa                                         >  1:5361675/1‑93 (MQ=255)
                                                  gCAAACAAACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGc                                       <  2:4267555/93‑1 (MQ=255)
                                                   cAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCg                                      <  1:5258927/93‑1 (MQ=255)
                                                    aaaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCgg                                     >  2:2667803/1‑93 (MQ=255)
                                                     aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCAGa                                    >  1:3755778/1‑93 (MQ=255)
                                                      aCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaa                                   <  1:1350534/93‑1 (MQ=255)
                                                       cAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaaa                                  <  1:6539225/93‑1 (MQ=255)
                                                       cAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaaa                                  <  2:2424587/93‑1 (MQ=255)
                                                        agccccGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTCCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                 <  2:3441494/90‑1 (MQ=255)
                                                         gACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACg                                >  1:3208531/1‑93 (MQ=255)
                                                         gACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACg                                >  2:1735483/1‑93 (MQ=255)
                                                         gACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACg                                >  2:4201653/1‑93 (MQ=255)
                                                          ccccGCCTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGc                               <  1:3595490/92‑1 (MQ=255)
                                                          aCCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGc                               >  1:6041570/1‑93 (MQ=255)
                                                            ccGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAACAAGCGGAAACGCTg                             >  1:2411704/1‑93 (MQ=255)
                                                            ccGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                             <  2:3744355/93‑1 (MQ=255)
                                                             cGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGt                            <  2:3369017/93‑1 (MQ=255)
                                                              gACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCCAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGta                           >  2:2235331/1‑93 (MQ=255)
                                                              gACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGta                           >  1:3089326/1‑93 (MQ=255)
                                                               aCTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtac                          >  1:349158/1‑93 (MQ=255)
                                                                 tctcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtacta                        <  2:1340576/93‑1 (MQ=255)
                                                                   tcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                      >  2:1046161/1‑93 (MQ=255)
                                                                    cGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                     <  2:3200070/93‑1 (MQ=255)
                                                                    cGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                     >  2:4066463/1‑93 (MQ=255)
                                                                    cGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                     <  1:4218620/93‑1 (MQ=255)
                                                                     gAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATc                    <  2:4558657/93‑1 (MQ=255)
                                                                     gAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATc                    >  1:1778653/1‑93 (MQ=255)
                                                                      aaTGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCg                   <  1:6632783/93‑1 (MQ=255)
                                                                       aTGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGt                  <  1:3923756/93‑1 (MQ=255)
                                                                        tGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                 >  1:3999511/1‑93 (MQ=255)
                                                                        tGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                 >  1:4925838/1‑93 (MQ=255)
                                                                          gTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaa               >  2:121285/1‑93 (MQ=255)
                                                                          gTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaa               >  1:2297869/1‑93 (MQ=255)
                                                                           ttCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaa              <  2:2006364/93‑1 (MQ=255)
                                                                           ttCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaa              <  1:6251021/93‑1 (MQ=255)
                                                                           ttCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaa              <  2:4925089/93‑1 (MQ=255)
                                                                           ttCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaa              <  1:3456916/93‑1 (MQ=255)
                                                                           ttCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaa              <  1:220101/93‑1 (MQ=255)
                                                                              ttGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGc           >  1:6489477/1‑93 (MQ=255)
                                                                                gTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctc         <  2:1534233/93‑1 (MQ=255)
                                                                                gTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctc         <  1:5197441/93‑1 (MQ=255)
                                                                                  ctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtg       <  1:2093190/93‑1 (MQ=255)
                                                                                   tcATTGCTACATTCATAAGTACGCATCCAAGAGCACGCTTAGTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgt      <  1:4834503/93‑1 (MQ=255)
                                                                                   tcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgt      >  1:2346090/1‑93 (MQ=255)
                                                                                    cATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg     >  1:5382324/1‑93 (MQ=255)
                                                                                     aTTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTgg    >  2:5872110/1‑93 (MQ=255)
                                                                                     aTTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTgg    >  2:2104753/1‑93 (MQ=255)
                                                                                       tGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCa  <  2:3299891/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCA  >  NC_000913/3483724‑3483903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑