BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,484,054	T→C	Y100H (TAC→CAC)	crp →	cyclic AMP receptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,484,054	0	T	C	100.0%	878.5 / NA	268	Y100H (TAC→CAC)	crp	cyclic AMP receptor protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (121/147); total (121/147)

Only 100 of 273 total aligned reads displayed.

TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA  >  NC_000913/3483964‑3484143

   aTTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaa                                                                                      >  2:4756941/1‑93 (MQ=255)

   aTTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaa                                                                                      <  2:4683282/93‑1 (MQ=255)

    ttGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaa                                                                                     <  1:2760786/93‑1 (MQ=255)

         gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAttt                                                                                <  1:5579913/93‑1 (MQ=255)

         gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAttt                                                                                >  2:3085171/1‑93 (MQ=255)

          aaCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTc                                                                               <  1:349372/93‑1 (MQ=255)

          aaCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTc                                                                               <  1:2661653/93‑1 (MQ=255)

            cTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGc                                                                             <  2:5804609/93‑1 (MQ=255)

             tGGTCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGcc                                                                            <  1:5172928/93‑1 (MQ=255)

             tGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGcc                                                                            >  2:147476/1‑93 (MQ=255)

             tGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGcc                                                                            <  1:4859521/93‑1 (MQ=255)

              gggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCa                                                                           <  2:2297869/93‑1 (MQ=255)

               ggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCaa                                                                          <  2:2589539/93‑1 (MQ=255)

               ggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCaa                                                                          >  2:5422763/1‑93 (MQ=255)

                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        <  2:6657573/93‑1 (MQ=255)

                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        <  1:3374705/93‑1 (MQ=255)

                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        <  2:4516085/93‑1 (MQ=255)

                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGCACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        >  2:3917391/1‑93 (MQ=255)

                  cTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTg                                                                       <  2:2966277/93‑1 (MQ=255)

                  cTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTg                                                                       >  2:1372712/1‑93 (MQ=255)

                   tGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAttca                                                                      <  1:6617708/93‑3 (MQ=255)

                   tGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGa                                                                      <  1:4365041/93‑1 (MQ=255)

                    gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAt                                                                     >  2:6329419/1‑93 (MQ=255)

                     tttGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAtt                                                                    <  1:4201653/93‑1 (MQ=255)

                       tGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCa                                                                  <  1:2178146/93‑1 (MQ=255)

                         aaGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAgg                                                                >  2:6570893/1‑93 (MQ=255)

                         aaGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAgg                                                                <  2:2499542/93‑1 (MQ=255)

                            agGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTaa                                                             >  2:4299492/1‑93 (MQ=255)

                              ggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAc                                                           <  1:3229999/93‑1 (MQ=255)

                                ccAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAccc                                                         >  2:32801/1‑93 (MQ=255)

                                 cAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                        >  1:4524529/1‑93 (MQ=255)

                                 cAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                        <  1:2820332/93‑1 (MQ=255)

                                 cAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                        <  2:6461773/93‑1 (MQ=255)

                                    gAACGTAGCGCATGGGTACGTGCGAAAGCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     <  2:5580388/93‑1 (MQ=255)

                                    gAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     <  1:3966677/93‑1 (MQ=255)

                                    gAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     <  1:78219/93‑1 (MQ=255)

                                    gAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     <  1:5259550/93‑1 (MQ=255)

                                     aaCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                    <  2:1635291/93‑1 (MQ=255)

                                     aaCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                    <  1:4671374/93‑1 (MQ=255)

                                      aCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                   >  2:112134/1‑93 (MQ=255)

                                       cGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                  <  2:5857236/93‑1 (MQ=255)

                                       cGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                  <  2:287038/93‑1 (MQ=255)

                                        gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  2:865005/93‑1 (MQ=255)

                                        gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  1:2104753/93‑1 (MQ=255)

                                         tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCt                                                >  2:6094085/1‑93 (MQ=255)

                                         tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCt                                                <  1:1316309/93‑1 (MQ=255)

                                           gcgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGa                                              <  2:2445715/93‑1 (MQ=255)

                                             gcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCCGATg                                            <  2:5097410/93‑1 (MQ=255)

                                              cATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGc                                           >  1:4961386/1‑93 (MQ=255)

                                               aTGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCg                                          <  1:3033213/93‑1 (MQ=255)

                                                tGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                         <  1:1027015/93‑1 (MQ=255)

                                                tGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                         <  1:5868874/93‑1 (MQ=255)

                                                agggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                         <  1:6395944/92‑1 (MQ=255)

                                                 gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                        >  1:1830297/1‑93 (MQ=255)

                                                 gggTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                        <  1:115836/93‑1 (MQ=255)

                                                  ggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt                                       >  2:3493481/1‑93 (MQ=255)

                                                     aCGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTc                                    <  1:2459363/93‑1 (MQ=255)

                                                       gTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTg                                  >  2:1497114/1‑93 (MQ=255)

                                                       gTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTg                                  <  1:6000612/93‑1 (MQ=255)

                                                        tGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGc                                 >  1:4468896/1‑93 (MQ=255)

                                                        tGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGc                                 <  1:6478910/93‑1 (MQ=255)

                                                           gAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcaca                              >  1:6560695/1‑93 (MQ=255)

                                                           gAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcaca                              <  1:1488301/93‑1 (MQ=255)

                                                              aaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                           >  1:1687228/1‑93 (MQ=255)

                                                              aaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                           >  2:3320027/1‑93 (MQ=255)

                                                               aCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATg                          <  1:251504/93‑1 (MQ=255)

                                                               aCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATg                          >  2:797310/1‑93 (MQ=255)

                                                                ccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         >  2:5950866/1‑93 (MQ=255)

                                                                ccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         >  2:5017333/1‑93 (MQ=255)

                                                                ccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         >  2:1312971/1‑93 (MQ=255)

                                                                ccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         >  2:1994334/1‑93 (MQ=255)

                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        >  2:3109564/1‑93 (MQ=255)

                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        <  1:970984/93‑1 (MQ=255)

                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        <  2:1293924/93‑1 (MQ=255)

                                                                  gCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcg                       <  2:3999511/93‑1 (MQ=255)

                                                                   ccTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcgc                      <  1:5946542/93‑1 (MQ=255)

                                                                    cTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGcgcg                     <  1:2117306/93‑1 (MQ=255)

                                                                     tgtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                    <  2:171011/93‑1 (MQ=255)

                                                                       tgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtcg                  >  1:3877716/1‑93 (MQ=255)

                                                                         aaGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCgtcgtc                >  2:4869937/1‑93 (MQ=255)

                                                                         aaGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCgtcgtc                >  2:5340479/1‑93 (MQ=255)

                                                                          aGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCt               >  1:6174896/1‑93 (MQ=255)

                                                                          aGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCt               >  1:4886506/1‑93 (MQ=255)

                                                                           gTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTg              >  2:5721326/1‑93 (MQ=255)

                                                                           gTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTg              <  2:6380924/93‑1 (MQ=255)

                                                                             ggCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCa            >  2:1876677/1‑93 (MQ=255)

                                                                              gCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCaa           >  2:6698312/1‑93 (MQ=255)

                                                                              gCTGAAATTTCGCACAAAAAATATCGCCAATTGAGTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCaa           <  1:2828033/93‑1 (MQ=255)

                                                                                 gAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTc        <  2:5227439/93‑1 (MQ=255)

                                                                                 gAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTc        <  2:4234719/93‑1 (MQ=255)

                                                                                  aaaTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCa       >  2:3546282/1‑93 (MQ=255)

                                                                                    aTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACt     <  1:4003892/93‑1 (MQ=255)

                                                                                    aTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACt     >  2:2725081/1‑93 (MQ=255)

                                                                                     tttCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt    >  2:4990859/1‑93 (MQ=255)

                                                                                     tttCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt    >  1:3753438/1‑93 (MQ=255)

                                                                                     tttCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt    <  1:5354550/93‑1 (MQ=255)

                                                                                     tttCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt    >  2:2120205/1‑93 (MQ=255)

                                                                                      ttCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTc   <  2:1830420/93‑1 (MQ=255)

                                                                                       tCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa  >  1:2336909/1‑93 (MQ=255)

                                                                                       tCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa  >  1:6685923/1‑93 (MQ=255)

TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA  >  NC_000913/3483964‑3484143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`