Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,222,944 4 bp→AGCA coding (2671‑2674/3093 nt) b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,222,9440CA100.0% 760.4 / NA 203Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (99/104);  total (99/104)
*NC_0009133,222,9450AG100.0% 659.3 / NA 203Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (99/104);  total (99/104)
*NC_0009133,222,9460AC100.0% 658.9 / NA 204Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (99/105);  total (99/105)
*NC_0009133,222,9470GA100.0% 732.4 / NA 204A892T (GCC→ACC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (99/105);  total (99/105)

Only 100 of 206 total aligned reads displayed.

GATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  >  NC_000913/3222863‑3223027
                                                                                 ||||                                                                                
gATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGGCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGAt                                                                          >  1:6245328/1‑93 (MQ=255)
gATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGAt                                                                          <  2:6520623/93‑1 (MQ=255)
 aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                         >  2:4559034/1‑93 (MQ=255)
 aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                         >  1:1411715/1‑93 (MQ=255)
 aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                         >  1:153554/1‑93 (MQ=255)
 aTCAGGTGGCGGATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                         >  1:9803/1‑93 (MQ=255)
  tCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGc                                                                        >  2:5229435/1‑93 (MQ=255)
  tCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGc                                                                        >  1:933064/1‑93 (MQ=255)
   cAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGcc                                                                       <  1:5510664/93‑1 (MQ=255)
    aGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACTTCATCGATATCTGGCGCAGCACCGTCGATGCCa                                                                      <  2:6546312/93‑1 (MQ=255)
    aGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCa                                                                      <  1:3661003/93‑1 (MQ=255)
    aGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCa                                                                      <  1:2736539/93‑1 (MQ=255)
    aGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCa                                                                      >  2:4953310/1‑93 (MQ=255)
     ggTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCAt                                                                     >  2:3221399/1‑93 (MQ=255)
       ttgCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGt                                                                   <  1:1530038/91‑1 (MQ=255)
        ggCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt                                                                  <  2:1721518/93‑1 (MQ=255)
        ggCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt                                                                  >  2:6655714/1‑93 (MQ=255)
        ggCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt                                                                  <  1:2178270/93‑1 (MQ=255)
         gCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTc                                                                 <  1:6553405/93‑1 (MQ=255)
          cGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCg                                                                <  1:6309863/93‑1 (MQ=255)
           gTATTACGGTCGTGGACCGGGCGAAAACTACGCCTACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCga                                                               >  2:3191653/1‑93 (MQ=255)
           gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCga                                                               >  1:2484827/1‑93 (MQ=255)
             aTTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgaga                                                             >  2:4728732/1‑93 (MQ=255)
              ttACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGaa                                                            >  1:4445086/1‑93 (MQ=255)
              ttACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGaa                                                            <  1:5319436/93‑1 (MQ=255)
               tACGGTGGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAAc                                                           <  1:5191061/93‑1 (MQ=255)
                aCGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACt                                                          >  2:4308047/1‑93 (MQ=255)
                aCGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACt                                                          <  2:5259734/93‑1 (MQ=255)
                 cGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTa                                                         >  2:3472806/1‑93 (MQ=255)
                  ggTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTAt                                                        <  1:486514/93‑1 (MQ=255)
                    tCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATcc                                                      <  2:2120102/93‑1 (MQ=255)
                     cggggACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATccc                                                     <  1:174580/90‑1 (MQ=255)
                     cGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATccc                                                     <  2:3881878/93‑1 (MQ=255)
                      gTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCt                                                    >  1:6177947/1‑93 (MQ=255)
                      ctGGACCGGGCGAAAACGACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCt                                                    <  2:1231221/92‑1 (MQ=255)
                       tGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCtt                                                   >  1:2812657/1‑93 (MQ=255)
                       tGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCtt                                                   >  1:5014931/1‑93 (MQ=255)
                       tGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCtt                                                   >  1:4620400/1‑93 (MQ=255)
                         gACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTcc                                                 <  1:4967166/93‑1 (MQ=255)
                         gACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTcc                                                 >  2:5048618/1‑93 (MQ=255)
                          aCCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTccc                                                >  1:4976064/1‑93 (MQ=255)
                              ggCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAg                                            >  1:1040657/1‑93 (MQ=255)
                                 gAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac                                         <  2:6184221/93‑1 (MQ=255)
                                 gAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac                                         <  2:6477221/93‑1 (MQ=255)
                                  aaaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaaca                                        >  2:1928044/1‑93 (MQ=255)
                                   aaccTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaa                                       <  1:3545434/90‑1 (MQ=255)
                                    aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                      <  2:3449150/93‑1 (MQ=255)
                                    aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                      <  1:3529770/93‑1 (MQ=255)
                                    aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                      >  1:230374/1‑93 (MQ=255)
                                    aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                      <  1:5454711/93‑1 (MQ=255)
                                    aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                      <  1:3015807/93‑1 (MQ=255)
                                    aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                      >  2:756959/1‑93 (MQ=255)
                                    aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                      <  2:4757160/93‑1 (MQ=255)
                                     aCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACg                                     <  1:3444166/93‑1 (MQ=255)
                                      cTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACgg                                    >  2:6128846/1‑93 (MQ=255)
                                      cTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACgg                                    <  2:5300789/93‑1 (MQ=255)
                                       tACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACgtt                                   >  1:4388843/1‑91 (MQ=255)
                                       tACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGt                                   >  2:6362489/1‑93 (MQ=255)
                                       tACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGt                                   <  1:602452/93‑1 (MQ=255)
                                        aCGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTa                                  <  2:256811/93‑1 (MQ=255)
                                          gCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAc                                >  2:1430155/1‑93 (MQ=255)
                                          gCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAc                                <  2:3930228/93‑1 (MQ=255)
                                          gCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAc                                <  2:3984628/93‑1 (MQ=255)
                                             gACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGCTGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGGAACCgc                             >  1:57972/1‑92 (MQ=255)
                                             gACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGt                             >  2:6030301/1‑93 (MQ=255)
                                              aCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTc                            >  2:180177/1‑93 (MQ=255)
                                                 gccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGc                         >  2:566786/1‑93 (MQ=255)
                                                 gccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGc                         >  1:4257204/1‑93 (MQ=255)
                                                  ccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGACCTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCa                        <  1:4922782/93‑1 (MQ=255)
                                                  ccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCa                        <  1:3139822/93‑1 (MQ=255)
                                                  ccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCa                        <  2:5365146/93‑1 (MQ=255)
                                                   cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAt                       <  1:859007/93‑1 (MQ=255)
                                                    agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg                      <  2:3301212/93‑1 (MQ=255)
                                                     gcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGt                     <  2:1868885/93‑1 (MQ=255)
                                                     gcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGt                     >  1:5579593/1‑93 (MQ=255)
                                                     gcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCCTCAGCATGt                     >  2:2398768/1‑93 (MQ=255)
                                                      cagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTc                    >  2:4142146/1‑93 (MQ=255)
                                                         gCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGc                 <  2:4033634/93‑1 (MQ=255)
                                                         gCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGc                 <  2:3135536/93‑1 (MQ=255)
                                                         gCTAACATCATAGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTACCCGTCAGCATGTCCGc                 <  2:5776719/93‑1 (MQ=255)
                                                           tAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg               >  2:6406978/1‑93 (MQ=255)
                                                           tAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg               >  1:6606192/1‑93 (MQ=255)
                                                             aaatcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGCACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa             >  1:1860253/3‑93 (MQ=255)
                                                             aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATGCCTTCCCGCAGAACAACGGTAACCGTGAGCATGTctgctgtg             >  1:690139/1‑91 (MQ=255)
                                                             aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa             >  1:1685428/1‑93 (MQ=255)
                                                              catcatCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAc            >  2:3901112/1‑93 (MQ=255)
                                                              catcatCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAc            >  2:4331021/1‑93 (MQ=255)
                                                               atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACg           >  2:5509402/1‑93 (MQ=255)
                                                                tcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg          >  1:5943858/1‑93 (MQ=255)
                                                                tcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg          >  2:5849849/1‑93 (MQ=255)
                                                                tcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg          >  1:418021/1‑93 (MQ=255)
                                                                tcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg          <  1:2337275/93‑1 (MQ=255)
                                                                 catcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGc         >  1:6670712/1‑93 (MQ=255)
                                                                  atcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCa        >  2:876765/1‑93 (MQ=255)
                                                                   tcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAc       <  2:5736259/93‑1 (MQ=255)
                                                                   tcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAc       <  2:2914400/93‑1 (MQ=255)
                                                                   tcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAc       >  1:1308555/1‑93 (MQ=255)
                                                                     gATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTg     <  1:3840708/93‑1 (MQ=255)
                                                                     gATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTg     >  1:1188168/1‑93 (MQ=255)
                                                                       tatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAc   >  2:2806384/1‑93 (MQ=255)
                                                                                 ||||                                                                                
GATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  >  NC_000913/3222863‑3223027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑