BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,575,446	+G	coding (912/942 nt)	gntR ←	GntR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,575,445	1	.	G	99.8%	1918.7 / ‑4.0	496	coding (913/942 nt)	gntR	GntR
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (280/215); total (280/216)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-01

Only 100 of 561 total aligned reads displayed.

CAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑GCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTG  >  NC_000913/3575350‑3575535

cAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca                                                                                             <  2:974596/96‑1 (MQ=255)

cAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca                                                                                             <  1:4284376/96‑1 (MQ=255)

cAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca                                                                                             <  2:529138/96‑1 (MQ=255)

 aaaTTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑g                                                                                           <  2:14861/96‑1 (MQ=255)

 aaaTTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑g                                                                                           <  1:1602478/96‑1 (MQ=255)

  aaTTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑gg                                                                                          <  1:12434538/96‑2 (MQ=255)

  aaTTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑gg                                                                                          <  1:13302973/96‑2 (MQ=255)

   aattGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑ggg                                                                                         >  1:11509267/3‑94 (MQ=255)

     ttGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑ggcgt                                                                                       <  2:668120/96‑5 (MQ=255)

       gtgGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTc                                                                                      <  1:2689713/96‑1 (MQ=255)

       gtgGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTc                                                                                      <  2:955377/96‑1 (MQ=255)

       gtgGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTc                                                                                      <  1:8917253/96‑1 (MQ=255)

         gggCTTAAATAGATCCGCCCGGTTACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAg                                                                                    <  1:10059783/96‑1 (MQ=255)

         gggCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAg                                                                                    >  1:10882471/1‑96 (MQ=255)

          ggCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAgc                                                                                   >  2:12849378/1‑96 (MQ=255)

          ggCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAgc                                                                                   <  2:13081422/96‑1 (MQ=255)

              tAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCa                                                                               <  2:8374942/96‑1 (MQ=255)

               aaaTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCaa                                                                              <  2:11776496/96‑1 (MQ=255)

               aaaTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCaa                                                                              >  2:1989086/1‑96 (MQ=255)

               aaaTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGGTCAGCGCCaa                                                                              >  2:444726/1‑96 (MQ=255)

                 aTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAAtn                                                                            <  1:5523895/96‑2 (MQ=255)

                 aTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATa                                                                            <  2:5173366/96‑1 (MQ=255)

                 aTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGGGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATa                                                                            >  2:10662306/1‑96 (MQ=255)

                  tAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATAc                                                                           >  1:6320547/1‑96 (MQ=255)

                   aGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACt                                                                          >  1:13848457/1‑96 (MQ=255)

                   aGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACt                                                                          <  1:3997150/96‑1 (MQ=255)

                    gATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTg                                                                         >  2:10877830/1‑96 (MQ=255)

                    gATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTg                                                                         >  2:8418225/1‑96 (MQ=255)

                     aTCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGc                                                                        >  1:6386454/1‑96 (MQ=255)

                         gCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCAt                                                                    >  2:9159707/1‑96 (MQ=255)

                           atggTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATcc                                                                  >  1:11286720/3‑96 (MQ=255)

                            cGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCg                                                                 >  2:6989867/1‑96 (MQ=255)

                               tGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGcgc                                                              <  1:7563677/96‑3 (MQ=255)

                                gACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCATCGCCAATACTGCCCATCCGCTCa                                                             >  1:10307545/1‑96 (MQ=255)

                                gACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCa                                                             >  1:7888833/1‑96 (MQ=255)

                                 aCAAGGTGAAGCCTAAATCTAACATTGACGGTGTCACAGATTCGCCACGAATCCGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAc                                                            <  1:2342748/96‑1 (MQ=255)

                                 aCAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAc                                                            <  2:6056056/96‑1 (MQ=255)

                                 aCAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAc                                                            >  2:7822152/1‑96 (MQ=255)

                                  cAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAcg                                                           >  2:3145328/1‑96 (MQ=255)

                                   aaGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCTcgc                                                          <  1:9680127/96‑1 (MQ=255)

                                   aaGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAcgc                                                          <  1:4523291/96‑1 (MQ=255)

                                   aaGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAcgc                                                          >  2:11111872/1‑96 (MQ=255)

                                    aGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAcgcg                                                         >  2:2349037/1‑96 (MQ=255)

                                    aGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAcgcg                                                         >  1:3750579/1‑96 (MQ=255)

                                     ggTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACgcgg                                                        >  1:4957643/1‑96 (MQ=255)

                                     ggTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACgcgg                                                        <  1:7255672/96‑1 (MQ=255)

                                       tGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACgcggcg                                                      >  2:8094566/1‑96 (MQ=255)

                                       tGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACgcggcg                                                      >  2:12086521/1‑96 (MQ=255)

                                        ggaaCCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGt                                                     >  1:10551098/3‑96 (MQ=255)

                                         aaaCCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTc                                                    >  1:7731015/1‑96 (MQ=255)

                                          aaCCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCa                                                   >  1:3342831/1‑96 (MQ=255)

                                            cttAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGc                                                 >  1:11041600/3‑96 (MQ=255)

                                            ccTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGc                                                 <  1:8763107/96‑1 (MQ=255)

                                             cTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCa                                                <  1:349325/96‑1 (MQ=255)

                                               aaaTCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACg                                              <  2:13448674/96‑1 (MQ=255)

                                                aaTCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGc                                             >  2:10566607/1‑96 (MQ=255)

                                                aaTCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGc                                             >  2:5789291/1‑96 (MQ=255)

                                                 aTCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCt                                            <  1:8506051/96‑1 (MQ=255)

                                                 aTCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCt                                            >  1:8319687/1‑96 (MQ=255)

                                                  tCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTc                                           >  1:5157873/1‑96 (MQ=255)

                                                  tCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTc                                           <  1:591971/96‑1 (MQ=255)

                                                  tCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTc                                           <  2:6217123/96‑1 (MQ=255)

                                                  tCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTc                                           >  1:4666183/1‑96 (MQ=255)

                                                     aaCATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCa                                        >  2:11629241/1‑96 (MQ=255)

                                                      aCATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCaa                                       <  1:14416477/96‑1 (MQ=255)

                                                      aCATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCaa                                       >  2:5607556/1‑96 (MQ=255)

                                                      aCATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCaa                                       >  1:1553712/1‑96 (MQ=255)

                                                       cATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAg                                      >  1:6618907/1‑96 (MQ=255)

                                                        aTTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGt                                     <  2:3482955/96‑1 (MQ=255)

                                                        aTTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGt                                     >  2:8752279/1‑96 (MQ=255)

                                                         ttttCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTc                                    <  1:5704065/96‑1 (MQ=255)

                                                          tttCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGtgg                                   <  1:13021690/96‑3 (MQ=255)

                                                          tttCGGTGTCACACATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCg                                   <  2:7276655/96‑1 (MQ=255)

                                                           ttCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTTACGCGGCGTCAGCACGCTCGCAAGTCGt                                  <  2:13910844/96‑1 (MQ=255)

                                                            tCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTg                                 <  2:7558223/96‑1 (MQ=255)

                                                            tCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTg                                 >  2:8180769/1‑96 (MQ=255)

                                                               gggtCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCt                              >  1:9386205/3‑96 (MQ=255)

                                                                  tCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGATGACGCGGCGTCAGCACGCTCGCAAGCCGTGGCTCCa                           >  1:2907054/1‑96 (MQ=255)

                                                                   cacaGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCAt                          >  2:11344510/1‑96 (MQ=255)

                                                                   cacaGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCAt                          <  2:1660208/96‑1 (MQ=255)

                                                                    acaGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATc                         <  2:2646925/96‑1 (MQ=255)

                                                                     caGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCa                        <  2:14181602/96‑1 (MQ=255)

                                                                       gATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCAcc                      <  1:2086984/96‑1 (MQ=255)

                                                                        aTTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCt                     >  1:12026419/1‑96 (MQ=255)

                                                                                 gAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGt            >  1:13679703/1‑96 (MQ=255)

                                                                                 gAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGt            >  2:3352743/1‑96 (MQ=255)

                                                                                 gAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGt            >  1:14072267/1‑96 (MQ=255)

                                                                                   aTACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCa          >  2:13704998/1‑96 (MQ=255)

                                                                                    tACGCGCTAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCAt         <  1:1869492/96‑1 (MQ=255)

                                                                                     aCGGGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATg        >  2:12575021/1‑96 (MQ=255)

                                                                                     aCGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATg        <  2:4917259/96‑1 (MQ=255)

                                                                                     aCGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATg        >  2:4607942/1‑96 (MQ=255)

                                                                                       gcgcCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCACGAc      <  2:10110159/96‑1 (MQ=255)

                                                                                         gcCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCg    >  2:12373968/1‑96 (MQ=255)

                                                                                         gcCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCg    >  1:3184849/1‑96 (MQ=255)

                                                                                         gcCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCg    >  1:9041399/1‑96 (MQ=255)

                                                                                         gcCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCg    >  1:6927593/1‑96 (MQ=255)

                                                                                          ccAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGt   >  2:6403973/1‑96 (MQ=255)

                                                                                           cagcagGCGTTCAGCGCCAATACTGCCCATCCGGTCACGCGGCGTCAGCACGCTCGCAAGTCGTGACTCCATCACCTGACCAATGTCATGACCggg  >  2:14088523/1‑94 (MQ=255)

                                                                                           cagcagGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTg  <  2:3836167/96‑1 (MQ=255)

CAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑GCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTG  >  NC_000913/3575350‑3575535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`