Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,006,483 +C coding (183/207 nt) b4554 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,006,4801.C97.4% 1567.2 / 25.0 417coding (186/207 nt)b4554hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (4/7);  new base C (221/184);  total (226/191)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 482 total aligned reads displayed.

AGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG  >  NC_000913/4006385‑4006574
                                                                                                 |                                                                                              
aGCGGCTCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑Gt                                                                                                 <  1:6459867/96‑1 (MQ=255)
aGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑Gt                                                                                                 >  2:3061881/1‑96 (MQ=255)
aGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑Gt                                                                                                 >  2:5721837/1‑96 (MQ=255)
  cggcgCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cc                                                                                              <  1:7480686/96‑1 (MQ=255)
   gtcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑ccc                                                                                             >  1:8659421/3‑96 (MQ=255)
    gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACCCTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccc                                                                                            <  2:3224062/96‑2 (MQ=255)
    gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccc                                                                                            <  2:11090180/96‑2 (MQ=255)
    gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccc                                                                                            <  2:9345158/96‑2 (MQ=255)
    gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccc                                                                                            >  1:14397567/1‑95 (MQ=255)
      gcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑ccccaa                                                                                          <  2:8375762/96‑4 (MQ=255)
       cgcCCCTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCCAcc                                                                                         <  1:10660948/96‑1 (MQ=255)
       cgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑ccccaac                                                                                         <  2:9821362/96‑5 (MQ=255)
         ccACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCCAaccc                                                                                       >  2:11716443/1‑96 (MQ=255)
         ccACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCCAaccc                                                                                       <  1:13958909/96‑1 (MQ=255)
          cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAAcccc                                                                                       >  2:5213073/1‑96 (MQ=255)
          cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAAcccc                                                                                       >  1:5523796/1‑96 (MQ=255)
          cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAAcccc                                                                                       >  2:7938292/1‑96 (MQ=255)
           aCTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCa                                                                                      >  2:5464347/1‑96 (MQ=255)
           aCTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCa                                                                                      <  1:9422235/96‑1 (MQ=255)
              gctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAAGAGC‑GTCCCCAACCCCACGc                                                                                   >  1:12386419/1‑96 (MQ=255)
               ctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGcc                                                                                  <  1:11978323/96‑1 (MQ=255)
                tgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGGC‑CCCAACCCCACGCCa                                                                                 <  2:12904732/96‑1 (MQ=255)
                 ggATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCaa                                                                                <  2:8893347/96‑1 (MQ=255)
                  gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAAc                                                                               <  2:6250215/96‑1 (MQ=255)
                  gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAAc                                                                               >  2:13967218/1‑96 (MQ=255)
                  gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAAc                                                                               <  1:12241910/96‑1 (MQ=255)
                  gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAAc                                                                               >  1:2627806/1‑96 (MQ=255)
                   aTTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAAcc                                                                              <  2:10713742/96‑1 (MQ=255)
                    ttCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCa                                                                             <  2:4855692/96‑1 (MQ=255)
                     tCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAg                                                                            <  1:2279730/96‑1 (MQ=255)
                     tCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAg                                                                            <  2:1442041/96‑1 (MQ=255)
                      cccAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGc                                                                           >  1:13598709/1‑96 (MQ=255)
                       ccAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGGC‑CCCAACCCCACGCCAACCAGca                                                                          >  1:169484/1‑96 (MQ=255)
                        cAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGcac                                                                         >  1:10299841/1‑96 (MQ=255)
                        cAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTGCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGcac                                                                         >  1:12988872/1‑96 (MQ=255)
                          gCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACaa                                                                       >  1:5657304/1‑96 (MQ=255)
                            cAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAata                                                                     >  1:4690884/1‑96 (MQ=255)
                            cAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAata                                                                     >  1:5840720/1‑96 (MQ=255)
                              aaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATg                                                                   >  2:7605486/1‑96 (MQ=255)
                              aaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATg                                                                   <  1:11650757/96‑1 (MQ=255)
                                 cAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAAt                                                                <  1:1975576/96‑1 (MQ=255)
                                  aaGTAAGCCGCGCCTGCCCCCTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATc                                                               <  2:169817/96‑1 (MQ=255)
                                   aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGACTCg                                                              <  2:12576085/96‑1 (MQ=255)
                                   aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCg                                                              <  2:1813590/96‑1 (MQ=255)
                                   aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCg                                                              >  2:7203147/1‑96 (MQ=255)
                                   aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCg                                                              >  1:10034643/1‑96 (MQ=255)
                                     tAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGcc                                                            <  1:9155985/96‑1 (MQ=255)
                                      aaGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCa                                                           <  2:13733318/96‑1 (MQ=255)
                                       aGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAg                                                          >  2:3687372/1‑96 (MQ=255)
                                        gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGt                                                         <  2:338295/96‑1 (MQ=255)
                                        gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGt                                                         <  1:9704987/96‑1 (MQ=255)
                                        gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGt                                                         <  1:7782484/96‑1 (MQ=255)
                                          cgcgCCTGCCCACTTCAACACTTAAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                       <  1:2980693/96‑1 (MQ=255)
                                           gcgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                                      <  2:3970458/96‑1 (MQ=255)
                                            cgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                                     <  1:10484123/96‑1 (MQ=255)
                                            cgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                                     >  1:7986817/1‑96 (MQ=255)
                                             gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGGACAATATGAATCGCCAGTCCGGt                                                    >  1:10128629/1‑96 (MQ=255)
                                               cTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                                  >  1:6892320/1‑96 (MQ=255)
                                                tGCCCACTTCCACACTTCAAACGCAATCCCTGAGCGGGAAAATAGC‑GTCCCCACCCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                                 <  1:4068998/96‑1 (MQ=255)
                                                tGCCCACTTCAACCCTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                                 <  2:3502030/96‑1 (MQ=255)
                                                tGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                                 >  2:1215072/1‑96 (MQ=255)
                                                 gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCGACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                                <  1:3111070/96‑1 (MQ=255)
                                                 gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                                >  2:6570231/1‑96 (MQ=255)
                                                  cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                               <  1:3463851/96‑1 (MQ=255)
                                                   ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAg                                              >  2:1338418/1‑96 (MQ=255)
                                                     actccaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGcc                                            <  2:2566037/96‑1 (MQ=255)
                                                      cttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCATCCCCACGCGAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                           <  1:12603412/96‑1 (MQ=255)
                                                      cttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                           >  2:13046441/1‑96 (MQ=255)
                                                        tcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAg                                         <  2:1941456/96‑1 (MQ=255)
                                                         cgacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGc                                        >  1:11102629/3‑96 (MQ=255)
                                                         caacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGc                                        <  2:2209687/96‑1 (MQ=255)
                                                          aacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCa                                       >  1:13377356/1‑96 (MQ=255)
                                                          aacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCa                                       >  2:1054826/1‑96 (MQ=255)
                                                          aacactccaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCa                                       <  2:9637291/96‑1 (MQ=255)
                                                             acttcaaACGCAATCACCGAGCGGGAAAATAGC‑GTCCCCACCCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCaaca                                    <  2:8153106/96‑3 (MQ=255)
                                                                 caaACGCAATCACTGAGCGGGGAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACg                                >  1:12861599/1‑96 (MQ=255)
                                                                 caaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACg                                <  1:14159854/96‑1 (MQ=255)
                                                                 caaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACg                                >  2:11414924/1‑96 (MQ=255)
                                                                 caaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACg                                <  2:4180760/96‑1 (MQ=255)
                                                                 caaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACg                                <  2:450918/96‑1 (MQ=255)
                                                                  aaaCGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGc                               <  2:10195636/96‑1 (MQ=255)
                                                                   aaCGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGcc                              <  1:7507994/96‑1 (MQ=255)
                                                                    aCGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCa                             >  1:4587549/1‑96 (MQ=255)
                                                                     cGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAc                            <  2:8659421/96‑1 (MQ=255)
                                                                      gCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                           <  1:9591017/96‑1 (MQ=255)
                                                                      gCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                           >  2:5995745/1‑96 (MQ=255)
                                                                        aaTCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgc                         <  1:3801520/96‑1 (MQ=255)
                                                                          tCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgc                       >  1:3825965/1‑96 (MQ=255)
                                                                           ctcTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGGAATAGACGCCAccgcgcc                      >  1:11782086/3‑96 (MQ=255)
                                                                            aCTGAGTGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg                     <  1:5145082/96‑1 (MQ=255)
                                                                            aCTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg                     >  2:12086768/1‑96 (MQ=255)
                                                                            aCTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg                     <  1:8572703/96‑1 (MQ=255)
                                                                              tGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAACCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccgcg                   <  2:2694721/96‑1 (MQ=255)
                                                                                 gCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATa                <  1:8218582/96‑1 (MQ=255)
                                                                                   gggAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAc              >  1:4340198/1‑96 (MQ=255)
                                                                                     gAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCg            >  1:2195964/1‑96 (MQ=255)
                                                                                      aaaaTAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGt           >  2:7021968/1‑96 (MQ=255)
                                                                                       aaaTAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTg          <  2:10036187/96‑1 (MQ=255)
                                                                                          tAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGtt       <  1:8934487/96‑1 (MQ=255)
                                                                                          tAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGtt       >  2:9501562/1‑96 (MQ=255)
                                                                                                 |                                                                                              
AGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG  >  NC_000913/4006385‑4006574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑