BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,115,116	+C	coding (2013/2028 nt)	b2974 ←	putative endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,115,115	1	.	C	99.5%	2575.7 / 0.3	651	coding (2014/2028 nt)	b2974	putative endoglucanase
Reads supporting (aligned to +/- strand): ref base . (0/3); new base C (247/400); total (248/403)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 703 total aligned reads displayed.

TCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGT  >  NC_000913/3115020‑3115205

tCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAt‑tt                                                                                             >  1:4436982/1‑96 (MQ=255)

 cGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTGAAGGATCAGCAGCGGCAT‑TT‑c                                                                                           >  2:1700046/1‑96 (MQ=255)

   gTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cct                                                                                         <  2:12565400/96‑3 (MQ=255)

   gTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cct                                                                                         <  2:4406643/96‑3 (MQ=255)

    tttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cctg                                                                                        <  2:951754/96‑4 (MQ=255)

     ttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cctgc                                                                                       <  1:857299/96‑5 (MQ=255)

     ttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cctgc                                                                                       <  2:583794/96‑5 (MQ=255)

       gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCgg                                                                                      >  2:13138372/1‑96 (MQ=255)

       gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCgg                                                                                      <  1:6284109/96‑1 (MQ=255)

        cTGGTCTCTGCACGCAGCGGGATTGCATAAGGATCGTTTCCCACCTGAGTCAAATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGa                                                                                     <  1:6001969/96‑1 (MQ=255)

        cTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGa                                                                                     >  1:7198969/1‑96 (MQ=255)

          ggaaTCTGCACGCAGCGGGATTGCATAAGGATCGTTAGCCACATGAGTCACATATTCAAAGCCGTTAAGGATGAGCAGCGGCAT‑TTCCTGCGGATc                                                                                   >  1:12393402/4‑96 (MQ=255)

          ggTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATc                                                                                   <  2:7151137/96‑1 (MQ=255)

          ggTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATc                                                                                   <  2:7327234/96‑1 (MQ=255)

             aTCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCgag                                                                                <  1:3148773/96‑1 (MQ=255)

               cagCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGGCACATATTCAAAGCCGTTAAGGAGCAGCAGCGGCATGTC‑CTGCGGATCGAGAt                                                                              >  1:3560903/3‑96 (MQ=255)

                tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATc                                                                             <  2:5990699/96‑1 (MQ=255)

                tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATc                                                                             >  1:7286218/1‑96 (MQ=255)

                tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATc                                                                             <  2:1959694/96‑1 (MQ=255)

                tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATc                                                                             <  1:2720451/96‑1 (MQ=255)

                tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATc                                                                             <  2:10367577/96‑1 (MQ=255)

                   aCGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGcc                                                                          <  1:2957675/96‑1 (MQ=255)

                    cGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCa                                                                         <  2:9061208/96‑1 (MQ=255)

                    cGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCa                                                                         >  1:10895738/1‑96 (MQ=255)

                     gCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCAt                                                                        <  1:1952025/96‑1 (MQ=255)

                      cAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCTGCAT‑TTCCTTCGGATCGAGATCGCCATa                                                                       <  1:13205743/96‑1 (MQ=255)

                      cAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATa                                                                       >  1:2229369/1‑96 (MQ=255)

                      cAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATa                                                                       <  1:10014663/96‑1 (MQ=255)

                       aGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATTTAGATCTCCATAg                                                                      <  1:13440659/96‑1 (MQ=255)

                       aGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAg                                                                      >  1:1364457/1‑96 (MQ=255)

                       aGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAg                                                                      <  2:12848135/96‑1 (MQ=255)

                        gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATaac                                                                     <  1:10619954/96‑3 (MQ=255)

                          gggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTa                                                                   <  1:9197495/96‑1 (MQ=255)

                            gATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACt                                                                 <  1:8982849/96‑1 (MQ=255)

                             aTTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACtt                                                                <  2:10706520/96‑1 (MQ=255)

                              ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTa                                                               <  2:10083885/96‑1 (MQ=255)

                              ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTa                                                               <  1:8329314/96‑1 (MQ=255)

                              ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTa                                                               <  1:7012386/96‑1 (MQ=255)

                                 cATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAAt                                                            >  2:8627210/1‑96 (MQ=255)

                                 cATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAAt                                                            <  1:6595215/96‑1 (MQ=255)

                                  aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATg                                                           >  2:8831049/1‑96 (MQ=255)

                                   tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGc                                                          <  2:2231416/96‑1 (MQ=255)

                                    aaGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCt                                                         >  1:4613718/1‑96 (MQ=255)

                                     aGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                        >  1:14409529/1‑96 (MQ=255)

                                        aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                     >  2:10594163/1‑96 (MQ=255)

                                        aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                     >  2:640888/1‑96 (MQ=255)

                                         tCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACa                                                    <  2:6147304/96‑1 (MQ=255)

                                          cGTGACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                   <  1:6749635/96‑1 (MQ=255)

                                           gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                                  >  2:2975138/1‑96 (MQ=255)

                                           gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                                  <  1:5015680/96‑1 (MQ=255)

                                            ttACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACagag                                                 <  2:5647498/96‑1 (MQ=255)

                                            ttACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACagag                                                 <  2:14344291/96‑1 (MQ=255)

                                             tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                                >  1:4211328/1‑96 (MQ=255)

                                             tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                                <  2:13375115/96‑1 (MQ=255)

                                             tACCCACCTGAGTCACATATTCAAAGCCGTTAAAGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                                >  2:14080800/1‑96 (MQ=255)

                                               cccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATg                                              <  2:12312393/96‑1 (MQ=255)

                                                ccACCTGAGTCACATAGTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAttc                                             >  1:4025786/1‑94 (MQ=255)

                                                 cACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGcc                                            <  1:13924991/96‑1 (MQ=255)

                                                  aCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGccc                                           <  2:2605740/96‑1 (MQ=255)

                                                  aCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCAGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGccc                                           <  2:11154017/96‑1 (MQ=255)

                                                   ccTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCg                                          >  1:10350853/1‑96 (MQ=255)

                                                    cTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGc                                         >  2:4072189/1‑96 (MQ=255)

                                                            caTATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATTGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCg                                 <  1:13026227/96‑1 (MQ=255)

                                                            caTATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCg                                 <  1:11520338/96‑1 (MQ=255)

                                                              tatTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGa                               >  2:7581497/1‑96 (MQ=255)

                                                               atTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAt                              <  2:9641835/96‑1 (MQ=255)

                                                               atTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAt                              <  2:12012056/96‑1 (MQ=255)

                                                                 tCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATgg                            <  2:6756607/96‑1 (MQ=255)

                                                                  cAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGa                           >  1:5301245/1‑96 (MQ=255)

                                                                   aaaGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                          <  1:11818710/96‑1 (MQ=255)

                                                                   aaaGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                          <  2:9324020/96‑1 (MQ=255)

                                                                   aaaGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAGGGaa                          >  2:7004208/1‑96 (MQ=255)

                                                                    aaGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAg                         <  1:14138492/96‑1 (MQ=255)

                                                                      gCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                       <  2:11451342/96‑1 (MQ=255)

                                                                        cGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGa                     <  2:4970980/96‑1 (MQ=255)

                                                                         gTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGaa                    <  2:1364457/96‑1 (MQ=255)

                                                                          ttAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                   <  1:11116996/96‑1 (MQ=255)

                                                                          ttAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                   >  2:3506462/1‑96 (MQ=255)

                                                                          ttAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                   <  2:11763733/96‑1 (MQ=255)

                                                                          ttAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                   <  1:6458078/96‑1 (MQ=255)

                                                                           tAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACg                  <  1:8022627/96‑1 (MQ=255)

                                                                           tAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACg                  >  1:7628524/1‑96 (MQ=255)

                                                                           tAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACg                  <  2:815124/96‑1 (MQ=255)

                                                                            aatgATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGc                 >  2:2434743/4‑96 (MQ=255)

                                                                               gATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgc              <  2:8380445/96‑1 (MQ=255)

                                                                                aTCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcg             >  2:5246295/1‑96 (MQ=255)

                                                                                  cagcagCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcgca           <  1:11374148/96‑2 (MQ=255)

                                                                                  cagcagCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCt           <  2:3270440/96‑1 (MQ=255)

                                                                                  cagcagCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCt           <  1:3352245/96‑1 (MQ=255)

                                                                                   agcagcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGGCGAACGCAGCGCTg          >  1:9533697/1‑96 (MQ=255)

                                                                                    gcagcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTgn         <  1:5659250/96‑2 (MQ=255)

                                                                                    gcagcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt         <  1:4766549/96‑1 (MQ=255)

                                                                                    gcagcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt         >  2:4716594/1‑96 (MQ=255)

                                                                                      agcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGttt       >  1:2584646/1‑96 (MQ=255)

                                                                                      agcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGttt       >  1:9710978/1‑96 (MQ=255)

                                                                                      agcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAGGGAAGTCGAACGCAGCGCTGttt       >  2:3880407/1‑96 (MQ=255)

                                                                                       gcGGCAT‑TTCCTGCGGATCGAGATCGCCATCGCTACGTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTc      <  1:12714983/96‑1 (MQ=255)

                                                                                       gcGGCAT‑TTCCTACGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATTCCCGCAAAATCCGGATGGAAGGCGAACGCAGCGCTGTTTc      <  1:12042575/96‑1 (MQ=255)

                                                                                        cGGCAT‑ATCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTcc     >  1:2473523/1‑96 (MQ=255)

                                                                                          gCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGCGATGCCCGCAAACTCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTg   <  1:1736358/96‑1 (MQ=255)

TCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGT  >  NC_000913/3115020‑3115205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`