BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,359	0	C	T	48.3%	2.6 / inf	1019	I179I (ATC→ATT)	yecD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (253/273); new base T (235/257); total (488/531)
Fisher's exact test for biased strand distribution p-value = 9.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-05
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 1054 total aligned reads displayed.

TCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCT  >  NC_000913/1949264‑1949454

tCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATc                                                                                                 <  2:2053265/96‑1 (MQ=255)

  cACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCaa                                                                                               <  2:4143927/96‑1 (MQ=255)

  cACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTaa                                                                                               >  2:1414256/1‑96 (MQ=255)

  cACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTaa                                                                                               >  2:12135323/1‑96 (MQ=255)

   aCCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTaat                                                                                              >  1:120457/1‑95 (MQ=255)

    ccgcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAAcc                                                                                             <  2:3535/96‑1 (MQ=255)

    ccgcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAAcc                                                                                             <  1:11561250/96‑1 (MQ=255)

     cgcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCa                                                                                            >  2:1930710/1‑96 (MQ=255)

      gcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCat                                                                                           >  1:2706730/1‑96 (MQ=255)

      gcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCat                                                                                           <  2:9810474/96‑1 (MQ=255)

       cccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCata                                                                                          <  1:8372133/96‑1 (MQ=255)

          gcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCt                                                                                       <  2:13633806/96‑1 (MQ=255)

          gcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCt                                                                                       <  1:3231575/96‑1 (MQ=255)

           cAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTa                                                                                      <  2:9525816/96‑1 (MQ=255)

             gtGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATTACAGTATCAACCATATCTAcc                                                                                    <  1:10395802/95‑1 (MQ=255)

             aTGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTAcc                                                                                    >  2:7918010/1‑96 (MQ=255)

              tGCCTGGTAACTTGGCTTTAATCTGGTGATTGCCGACGCCGCCTGTAGTGCCGCCAGCGCCCAGCAGCACAATAACAGTATCAACCATATCTAccc                                                                                   <  2:499620/96‑1 (MQ=255)

              tGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGAGCCGCCAGCGCCGAGCAGCACAATAACAGGATCAACCATATCTAccc                                                                                   >  2:5815592/1‑96 (MQ=255)

                ccTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCcgc                                                                                 <  1:6505734/96‑1 (MQ=255)

                 cTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCcgcg                                                                                <  1:2538061/96‑1 (MQ=255)

                  tGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGAATCAACCATATCTACCCgcgc                                                                               >  2:2299840/1‑96 (MQ=255)

                  tGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCgcgc                                                                               >  2:11281058/1‑96 (MQ=255)

                    ggAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCAt                                                                             <  2:5229155/96‑1 (MQ=255)

                     gAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATc                                                                            <  1:3465261/96‑1 (MQ=255)

                     gAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATc                                                                            <  2:6026976/96‑1 (MQ=255)

                     gAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATc                                                                            >  2:4013007/1‑96 (MQ=255)

                      aaCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCg                                                                           <  2:1023498/96‑1 (MQ=255)

                      aaCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCg                                                                           >  1:8149701/1‑96 (MQ=255)

                      aaCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCg                                                                           <  1:5266351/96‑1 (MQ=255)

                      aaCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCg                                                                           >  2:4737372/1‑96 (MQ=255)

                       aCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGc                                                                          >  1:3080601/1‑96 (MQ=255)

                       aCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGc                                                                          >  1:7392401/1‑96 (MQ=255)

                         ttGGCTTTAATCTGGTGATTGCCGAAGAAGCCTGTAGAGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGccc                                                                        >  1:14193906/1‑96 (MQ=255)

                             cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgtgt                                                                    <  1:4980795/96‑1 (MQ=255)

                             cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgtgt                                                                    <  2:6368425/96‑1 (MQ=255)

                              tttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGtgtg                                                                   <  2:3189158/96‑1 (MQ=255)

                              tttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGtgtg                                                                   <  2:1615465/96‑1 (MQ=255)

                               ttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGc                                                                  >  2:10441798/1‑96 (MQ=255)

                                   tCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAg                                                              <  2:13341635/96‑1 (MQ=255)

                                    cTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGc                                                             <  1:6627054/96‑1 (MQ=255)

                                     tagTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCg                                                            >  1:10631758/3‑96 (MQ=255)

                                       gTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCACGCATCGCCCGTGTGCGTAGCGTg                                                          >  1:9774792/1‑96 (MQ=255)

                                        tGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTgg                                                         <  1:5480165/96‑1 (MQ=255)

                                        tGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTgg                                                         <  2:1387234/96‑1 (MQ=255)

                                         gATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGa                                                        <  1:2213953/96‑1 (MQ=255)

                                          aTTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGaa                                                       <  1:8067978/96‑1 (MQ=255)

                                              ccGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAgaga                                                   >  1:12578533/1‑96 (MQ=255)

                                                gAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATc                                                 >  2:12989333/1‑96 (MQ=255)

                                                gAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATc                                                 >  2:7665158/1‑96 (MQ=255)

                                                gAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATc                                                 >  2:2740736/1‑96 (MQ=255)

                                                 aaGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATcc                                                >  1:854230/1‑96 (MQ=255)

                                                  aGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCt                                               >  2:10998206/1‑96 (MQ=255)

                                                  aGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATACCAGCATTAATCATATCTACCCGCGCATCGCCCGCGTGCGTAGCGTGGAAGAGATCCt                                               >  2:12619908/1‑96 (MQ=255)

                                                   gACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCtt                                              <  1:7855080/96‑2 (MQ=255)

                                                      gCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAAc                                           >  2:10716303/1‑96 (MQ=255)

                                                      gCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAAc                                           >  2:9332769/1‑96 (MQ=255)

                                                      gCCTGTAGTGCCGCCAGCGCCGAACAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttcac                                           <  2:10077811/96‑5 (MQ=255)

                                                        cTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAAcgc                                         <  1:2928558/96‑1 (MQ=255)

                                                         tGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCAcgcg                                        >  1:7608233/1‑96 (MQ=255)

                                                         tGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCAcgcg                                        >  1:13694753/1‑96 (MQ=255)

                                                            aGTGCCGCCAGCGCCGAGCAGCACAATAACAGTACCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTa                                     <  2:14362780/96‑1 (MQ=255)

                                                            aGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTa                                     <  2:13993822/96‑1 (MQ=255)

                                                             gTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTAt                                    >  2:7101872/1‑96 (MQ=255)

                                                             gTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTAt                                    <  2:8817895/96‑1 (MQ=255)

                                                              tGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATg                                   <  1:7158966/96‑1 (MQ=255)

                                                              tGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATg                                   <  1:14009218/96‑1 (MQ=255)

                                                                gcgccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTAACGCGCTATGAt                                 >  1:11765130/2‑96 (MQ=255)

                                                                ccgccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGAt                                 <  1:11348180/96‑1 (MQ=255)

                                                                 cgccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAtt                                <  1:3676479/96‑1 (MQ=255)

                                                                   ccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTa                              <  2:4555048/96‑1 (MQ=255)

                                                                   ccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTa                              >  2:251507/1‑96 (MQ=255)

                                                                      gcgcCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACAt                           <  1:8769096/96‑1 (MQ=255)

                                                                      gcgcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACAt                           >  2:6628556/1‑96 (MQ=255)

                                                                       cgcCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATc                          >  1:9473758/1‑96 (MQ=255)

                                                                       caccGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATc                          >  1:11050280/3‑96 (MQ=255)

                                                                        gcCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGGAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCg                         >  1:10010070/1‑96 (MQ=255)

                                                                         ccGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCgg                        <  1:4171207/96‑1 (MQ=255)

                                                                         ccGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCgg                        >  1:6011097/1‑96 (MQ=255)

                                                                          cGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGCTATGATTTACATCGGt                       >  1:4716953/1‑96 (MQ=255)

                                                                          cGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGt                       >  2:376853/1‑96 (MQ=255)

                                                                           gAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTc                      >  1:5718871/1‑96 (MQ=255)

                                                                           gAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTc                      <  2:2973501/96‑1 (MQ=255)

                                                                            agcaacACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCCCGCGTTATGATTTACATCGGTCt                     <  1:6165935/96‑1 (MQ=255)

                                                                             gcaacaCAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCtg                    >  1:10768196/1‑95 (MQ=255)

                                                                                acacaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCg                 <  1:6908558/95‑1 (MQ=255)

                                                                                  acaATAACCGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCa               >  1:12971574/1‑96 (MQ=255)

                                                                                  acaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCa               <  1:4528355/96‑1 (MQ=255)

                                                                                  acaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCa               <  2:8547289/96‑1 (MQ=255)

                                                                                  acaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCa               >  1:1662547/1‑96 (MQ=255)

                                                                                   caATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACACGTTATGATTTACATCGGTCTGCTGCaa              >  1:12258004/1‑96 (MQ=255)

                                                                                    aaTAACAGTATCAACCATATCTCCCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAAt             <  2:5664365/96‑1 (MQ=255)

                                                                                     aTAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATg            <  2:11824219/96‑1 (MQ=255)

                                                                                     aTAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATg            <  1:1928027/96‑1 (MQ=255)

                                                                                     aTAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATg            >  1:9117786/1‑96 (MQ=255)

                                                                                       aaCAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATAGGTCTACCGCAATGGt          >  1:12772221/1‑96 (MQ=255)

                                                                                       aaCAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCAGGCGTTATGATTTACATCGGTCTGCCGCAATGGt          <  2:11596805/96‑1 (MQ=255)

                                                                                        aCAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTc         <  1:1532994/96‑1 (MQ=255)

                                                                                        aCAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTc         <  1:13417154/96‑1 (MQ=255)

                                                                                         cAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCg        <  2:8186169/96‑1 (MQ=255)

                                                                                         cAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCg        <  1:3933001/96‑1 (MQ=255)

TCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCT  >  NC_000913/1949264‑1949454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`