Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,978,702 C→T S68F (TCC→TTC)  yifK → putative amino acid/amine transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,978,7020CT99.7% 2309.0 / NA 599S68F (TCC→TTC) yifKputative amino acid/amine transport protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (348/248);  total (349/250)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 637 total aligned reads displayed.

CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTA  >  NC_000913/3978609‑3978794
                                                                                             |                                                                                            
tccTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTtcc                                                                                            >  1:11371432/2‑96 (MQ=255)
cccTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTtcc                                                                                            <  2:11815079/96‑1 (MQ=255)
cccTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTtcc                                                                                            <  1:13527601/96‑1 (MQ=255)
cccTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTtcc                                                                                            <  2:1240072/96‑1 (MQ=255)
   tGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCg                                                                                         <  1:2012116/96‑1 (MQ=255)
    taaaTGGGCCGGACCATCCGTATTGTTGGCCTATATTATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATTTTGTTCCTCGa                                                                                        <  2:9284381/95‑1 (MQ=255)
      aacgggccgggccATCCGTATTGTTGGCCCATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAac                                                                                      <  1:1390152/93‑1 (MQ=255)
       aTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAacc                                                                                     >  1:10007160/1‑96 (MQ=255)
       aTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAacc                                                                                     <  2:7524057/96‑1 (MQ=255)
       aTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAacc                                                                                     >  1:8082670/1‑96 (MQ=255)
       aTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAAGGGGCGAAATGTTGTTCCTCGAacc                                                                                     >  2:1731831/1‑96 (MQ=255)
         gggccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccgg                                                                                   >  2:1465527/1‑96 (MQ=255)
         gggccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccgg                                                                                   <  1:3115326/96‑1 (MQ=255)
         gggccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccgg                                                                                   >  1:5076217/1‑96 (MQ=255)
          ggccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggt                                                                                  >  1:8468440/1‑96 (MQ=255)
           gtcgggccATCCGTATTGTTGGACTATATGATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCACTGGGCGAAATGTTGTTCCTCGAaccggtt                                                                                 >  1:12081792/3‑96 (MQ=255)
           gccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAACGTTGTTCCTCGAaccggtt                                                                                 <  2:12094809/96‑1 (MQ=255)
            ccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtta                                                                                >  2:712374/1‑96 (MQ=255)
             cgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttac                                                                               >  2:3618615/1‑96 (MQ=255)
             cgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttac                                                                               >  2:6629930/1‑96 (MQ=255)
              gggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttacc                                                                              >  1:3795740/1‑96 (MQ=255)
                gccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccgg                                                                            >  1:1707993/1‑96 (MQ=255)
                   aTCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc                                                                         <  1:14118810/96‑1 (MQ=255)
                   aTCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc                                                                         >  2:4326812/1‑96 (MQ=255)
                    tCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcg                                                                        <  1:6148932/96‑1 (MQ=255)
                    gccGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcg                                                                        <  1:14332459/95‑1 (MQ=255)
                     ccGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgt                                                                       <  1:13205589/96‑1 (MQ=255)
                     ccGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgt                                                                       <  2:685804/96‑1 (MQ=255)
                     ccGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgt                                                                       <  2:3975422/96‑1 (MQ=255)
                      cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgtt                                                                      <  1:8777958/96‑1 (MQ=255)
                       gTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc                                                                     <  2:6021842/96‑1 (MQ=255)
                       gTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc                                                                     <  2:8915782/96‑1 (MQ=255)
                        tATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGttcgttcg                                                                    >  2:10599282/1‑96 (MQ=255)
                        tATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGttcgttcg                                                                    <  2:6487668/96‑1 (MQ=255)
                         aTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGc                                                                   >  1:8276740/1‑96 (MQ=255)
                         aTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGc                                                                   >  2:5626218/1‑96 (MQ=255)
                          ttgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGcc                                                                  <  1:7124360/96‑1 (MQ=255)
                           tgttgACCTATAGCCTCGCCGGGCTGTTCGTCTTTTTCATCATGGGTTCAATGGGCGAAATTTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCg                                                                 <  1:5209758/96‑1 (MQ=255)
                             ttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCAAACCGGTTACCGGCTCGTTCGCCGtt                                                               >  1:7787998/1‑96 (MQ=255)
                              tgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGttt                                                              <  2:4745929/96‑1 (MQ=255)
                                   tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcg                                                         <  2:4184829/96‑1 (MQ=255)
                                   tataTCATCGCCGGGCTGTTCGTCTTTATCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcg                                                         <  2:2895523/96‑1 (MQ=255)
                                    atatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcgc                                                        >  2:49737/1‑96 (MQ=255)
                                    atatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcgc                                                        <  2:6221157/96‑1 (MQ=255)
                                     tatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCa                                                       <  2:3795740/96‑1 (MQ=255)
                                     gatcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCa                                                       <  1:488187/95‑1 (MQ=255)
                                      atcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCAt                                                      >  1:4754245/1‑96 (MQ=255)
                                      atcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAAGGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCTCCGTTTATGCGCAt                                                      >  2:5405581/1‑96 (MQ=255)
                                       tcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATc                                                     >  1:9158093/1‑96 (MQ=255)
                                       taatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATc                                                     >  1:11743003/3‑96 (MQ=255)
                                        catcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCg                                                    >  1:5425915/1‑96 (MQ=255)
                                        catcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGGTTATGCGCATCg                                                    >  1:9918266/1‑96 (MQ=255)
                                        catcGCCGGGCTGTTCGTCTTTTACGTCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCg                                                    <  1:5814821/96‑1 (MQ=255)
                                              cGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTatat                                              >  2:12703168/1‑96 (MQ=255)
                                                ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGa                                            <  2:10784298/96‑1 (MQ=255)
                                                ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGa                                            >  1:2870065/1‑96 (MQ=255)
                                                ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGa                                            <  1:11920196/96‑1 (MQ=255)
                                                 gCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAg                                           <  1:9621399/96‑1 (MQ=255)
                                                  ctcttCGTCTTTTTCATCATGCGTTTAATGGGCGAAATGTTGTTCCTCGAACCGGTTCCCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc                                          <  1:11177546/93‑1 (MQ=255)
                                                  cTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc                                          >  2:3315574/1‑96 (MQ=255)
                                                    gTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGccc                                        >  1:4307824/1‑96 (MQ=255)
                                                    gTTCGGCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAAccc                                        >  1:416773/1‑96 (MQ=255)
                                                     ttCGTCTTTTTCATCATTCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCg                                       <  1:13728467/96‑1 (MQ=255)
                                                     ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCg                                       >  2:2771458/1‑96 (MQ=255)
                                                     ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCg                                       <  2:13402681/96‑1 (MQ=255)
                                                     ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCg                                       >  1:12483230/1‑96 (MQ=255)
                                                      tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGt                                      >  2:5814101/1‑96 (MQ=255)
                                                      tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGt                                      <  1:4990127/96‑1 (MQ=255)
                                                        gTCTTTTTCATCATGCGTGCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCTGATCGTTATATTAGCCCGTTc                                    >  1:6524850/1‑96 (MQ=255)
                                                         tCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGGTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCt                                   >  1:103946/1‑96 (MQ=255)
                                                          cTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCtt                                  >  1:12663608/1‑96 (MQ=255)
                                                           tttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCttt                                 <  2:9568001/96‑1 (MQ=255)
                                                           tttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGGTCttt                                 >  2:10267093/1‑96 (MQ=255)
                                                             tttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTgg                               >  2:11687838/1‑96 (MQ=255)
                                                              ttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGc                              >  2:10093428/1‑96 (MQ=255)
                                                              ttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGc                              <  1:6692818/96‑1 (MQ=255)
                                                               tccacatGGGTTTAATGGGCGAAATGTTTTTCCCCGAACCGGTTACCGGTTCGTTCGCCGTTTATTCGCATCGTTATATGAGCCCGTTCTTTGGCt                             <  2:8001647/92‑1 (MQ=255)
                                                               tcatcaTGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCt                             <  2:14286163/96‑1 (MQ=255)
                                                                catcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTa                            >  2:1984716/1‑96 (MQ=255)
                                                                   catGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGATTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtct                         >  2:11191617/1‑96 (MQ=255)
                                                                    aagCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtctc                        >  1:9341390/3‑96 (MQ=255)
                                                                      gCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAc                      >  2:2982117/1‑96 (MQ=255)
                                                                        gTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCg                    >  1:3455798/1‑96 (MQ=255)
                                                                         ttCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGc                   >  2:8514293/1‑96 (MQ=255)
                                                                          tCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATTAGCCCGTTCTTTGGCTATCTCACCGcc                  >  1:7483784/1‑96 (MQ=255)
                                                                          tCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGcc                  >  2:7235959/1‑96 (MQ=255)
                                                                          tCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGcc                  <  1:12933812/96‑1 (MQ=255)
                                                                          tCAATGGGCGAAATGTTGTTCCTCGAACAGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGcc                  <  2:8128848/96‑1 (MQ=255)
                                                                           cAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCt                 >  2:2340300/1‑96 (MQ=255)
                                                                               gggCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTc             <  2:5132406/96‑1 (MQ=255)
                                                                                ggCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCt            <  2:2095910/96‑1 (MQ=255)
                                                                                ggCGAAATGTTGTGCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCt            >  1:10946195/1‑96 (MQ=255)
                                                                                   gAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTAc         >  2:13005518/1‑96 (MQ=255)
                                                                                    aaaTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACt        <  2:7820111/96‑1 (MQ=255)
                                                                                    aaaTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACt        >  2:3494971/1‑96 (MQ=255)
                                                                                     agtgttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGCGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTg       >  1:10624935/3‑96 (MQ=255)
                                                                                      attttgtTCCTCGACCCGGTTACGGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg      <  1:4389048/93‑1 (MQ=255)
                                                                                      aTGTTGTACCTTGATCCGGTTCCCGGTTCGTTCGCCTTGTATGCGCATCGTTATATGAGCCTGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg      <  1:10109521/96‑1 (MQ=255)
                                                                                        gttgttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGtt    <  2:1378658/96‑1 (MQ=255)
                                                                                         ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGttt   >  1:2352923/1‑96 (MQ=255)
                                                                                             |                                                                                            
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTA  >  NC_000913/3978609‑3978794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑