BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,614,238	G→T	L133I (CTA→ATA)	yjjI ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,614,238	0	G	T	99.8%	2084.4 / NA	527	L133I (CTA→ATA)	yjjI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (266/259); total (268/259)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 546 total aligned reads displayed.

GGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCA  >  NC_000913/4614145‑4614330

  ccGATATTGGCGTGCATAAAGGCGTCTGGCAGGGGTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAAtt                                                                                          >  2:7460434/1‑96 (MQ=255)

  cagATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAAtt                                                                                          >  1:529008/3‑96 (MQ=255)

   cGATATTGGCGTGCATAAAGGCGTCTGGCAGGGGTATGTCGAGGTAAAGCCAGAAACGTTTTATTCGAACATCGATCTAGTCTTGTGTTATAATTc                                                                                         >  1:11234722/1‑96 (MQ=255)

    gATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTTTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCt                                                                                        >  1:10199316/1‑96 (MQ=255)

    gATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCt                                                                                        <  2:13368199/96‑1 (MQ=255)

    gATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCt                                                                                        <  1:2496839/96‑1 (MQ=255)

    gATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGACCTCGTCTTGTGTTATAATTCt                                                                                        >  2:1881251/1‑96 (MQ=255)

      tatTGGCGTGCATAAAGGCGTCTGGCAGGGGTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTaa                                                                                      >  2:6691802/1‑96 (MQ=255)

        ttGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACa                                                                                    <  1:4461736/96‑1 (MQ=255)

          ggCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATa                                                                                  >  2:9709357/1‑96 (MQ=255)

            cGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACg                                                                                >  2:451977/1‑96 (MQ=255)

             gTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACgg                                                                               >  2:8232666/1‑96 (MQ=255)

               gCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGtt                                                                             <  2:10599696/96‑1 (MQ=255)

               gCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGtt                                                                             <  2:5546256/96‑1 (MQ=255)

                cATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTg                                                                            <  1:4119413/96‑1 (MQ=255)

                cATAAAGGCGTCTGGCAGGGTTCTGTCGAGCTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTg                                                                            <  2:14104051/96‑1 (MQ=255)

                 aTAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGc                                                                           <  2:3544734/96‑1 (MQ=255)

                   aaaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGcaa                                                                         <  1:5519693/96‑1 (MQ=255)

                    aaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGcaac                                                                        <  2:4943813/96‑1 (MQ=255)

                    aaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGcaac                                                                        >  1:356589/1‑96 (MQ=255)

                       gCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCaacaac                                                                     <  1:10469134/96‑1 (MQ=255)

                       gCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCaacaac                                                                     <  1:4883655/96‑1 (MQ=255)

                       gCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCaacaac                                                                     <  1:2829604/96‑1 (MQ=255)

                        cGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACg                                                                    <  2:6962939/96‑1 (MQ=255)

                        cGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATAAGGTTGCAACAACg                                                                    <  1:10976772/96‑1 (MQ=255)

                         gTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGc                                                                   >  2:12643166/1‑96 (MQ=255)

                          tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACCTACGGTTGCAACAACGCa                                                                  <  1:1701818/96‑1 (MQ=255)

                          tCTGGCAGGGGTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCa                                                                  >  2:9511781/1‑96 (MQ=255)

                             ggCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATcc                                                               <  2:2220298/96‑1 (MQ=255)

                              gCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCa                                                              >  2:580586/1‑96 (MQ=255)

                               cAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAg                                                             <  1:8790012/96‑1 (MQ=255)

                               cAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAg                                                             >  2:11461957/1‑96 (MQ=255)

                                aGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCACCCAGt                                                            >  1:3397014/1‑96 (MQ=255)

                                 gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGtt                                                           >  1:4731643/1‑96 (MQ=255)

                                  ggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTg                                                          >  1:13780593/1‑96 (MQ=255)

                                   gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGc                                                         >  2:7928148/1‑96 (MQ=255)

                                   gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGc                                                         <  1:7642515/96‑1 (MQ=255)

                                      cTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGccct                                                      <  1:11420765/96‑2 (MQ=255)

                                       tGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTCGCAACAACGCATCCAGTTGCCCCa                                                     <  1:14050142/96‑1 (MQ=255)

                                          cGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGt                                                  <  2:2811351/96‑1 (MQ=255)

                                            aGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAg                                                <  1:12201102/96‑1 (MQ=255)

                                            aGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAg                                                >  1:9247972/1‑96 (MQ=255)

                                             ggTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACCACGCATCCAGTTGCCCCAGGTAGa                                               <  1:2205331/96‑1 (MQ=255)

                                             ggTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGa                                               <  1:580586/96‑1 (MQ=255)

                                              gTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAc                                              <  1:9679385/96‑1 (MQ=255)

                                               tAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAcc                                             <  2:2297308/96‑1 (MQ=255)

                                                  cGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGc                                          <  1:9005398/96‑1 (MQ=255)

                                                   gCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAAGAACGCATCCAGTTGCCCCAGGTAGACCGGCa                                         >  1:11885090/1‑96 (MQ=255)

                                                    ccAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCAt                                        >  1:7415398/1‑96 (MQ=255)

                                                     cAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATc                                       <  2:6822174/96‑1 (MQ=255)

                                                     cAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATAAGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATc                                       <  2:2586035/96‑1 (MQ=255)

                                                      aGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCTAGGTAGACCGGCATCg                                      >  1:12787623/1‑96 (MQ=255)

                                                      aGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCg                                      <  1:8006816/96‑1 (MQ=255)

                                                      aGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCg                                      >  1:1951167/1‑96 (MQ=255)

                                                       gAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGa                                     >  1:6830390/1‑96 (MQ=255)

                                                          aCGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCTCCAGGCAGACCGGCATCGAtga                                  >  1:13066861/1‑95 (MQ=255)

                                                           cGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtgtg                                 <  2:3711565/96‑1 (MQ=255)

                                                             ttttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGAc                               <  1:12308589/96‑1 (MQ=255)

                                                              tttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGAcc                              >  2:3083522/1‑96 (MQ=255)

                                                              tttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGAcc                              >  2:8863951/1‑96 (MQ=255)

                                                              tttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGAcc                              <  1:7795587/96‑1 (MQ=255)

                                                               ttATTCGAACCTCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCg                             <  2:6377913/96‑1 (MQ=255)

                                                               ttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGCCCGGCATCGATGTGACCg                             <  1:6405481/96‑1 (MQ=255)

                                                                 aTTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGAc                           <  2:4217710/96‑1 (MQ=255)

                                                                 aTTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGAc                           <  2:14377407/96‑1 (MQ=255)

                                                                  ttCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGGCCGACg                          >  1:6130100/1‑96 (MQ=255)

                                                                  ttCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACg                          >  2:3421791/1‑96 (MQ=255)

                                                                  ttCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACg                          <  2:12423381/96‑1 (MQ=255)

                                                                   tCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACgt                         >  2:3195646/1‑95 (MQ=255)

                                                                   tCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACgg                         <  1:1881251/96‑1 (MQ=255)

                                                                    cGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGt                        <  1:3670242/96‑1 (MQ=255)

                                                                    cGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGGTGCCCCAGGTAGACCGGCATCGATGTGAACGACGGt                        >  2:11923677/1‑96 (MQ=255)

                                                                     gAACATCGATCTCGTCTTGTGTTATAATTCTAACATGCGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTa                       >  1:12938013/1‑96 (MQ=255)

                                                                      aaCATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCTTCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGttc                      <  1:10559952/96‑3 (MQ=255)

                                                                      aaCATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTAc                      <  2:1128481/96‑1 (MQ=255)

                                                                       ataTCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACg                     >  2:10909293/3‑96 (MQ=255)

                                                                       aCATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACg                     <  1:7273223/96‑1 (MQ=255)

                                                                       aCATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACg                     <  2:10743422/96‑1 (MQ=255)

                                                                       aCATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACg                     <  2:4679855/96‑1 (MQ=255)

                                                                          tCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtgg                  <  1:7032514/96‑1 (MQ=255)

                                                                          tCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtgg                  >  2:85673/1‑96 (MQ=255)

                                                                          tCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtgg                  >  2:5076714/1‑96 (MQ=255)

                                                                            gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggtg                <  1:10428804/96‑1 (MQ=255)

                                                                            gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggtg                <  2:8431783/96‑1 (MQ=255)

                                                                            gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggtg                >  1:14341180/1‑96 (MQ=255)

                                                                            gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggtg                >  1:8370610/1‑96 (MQ=255)

                                                                              tctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGAACGCggtggt              >  1:13661362/1‑96 (MQ=255)

                                                                               ctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTa             >  2:909723/1‑96 (MQ=255)

                                                                                 cGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTaaa           <  2:12224248/96‑1 (MQ=255)

                                                                                  gTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAg          >  2:1302089/1‑96 (MQ=255)

                                                                                   tCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGa         >  2:7197808/1‑96 (MQ=255)

                                                                                    cTTGTGTTATGATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGaa        <  2:13199173/96‑1 (MQ=255)

                                                                                    cTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGaa        >  2:1762239/1‑96 (MQ=255)

                                                                                     ttGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGatt       <  1:3092007/96‑3 (MQ=255)

                                                                                         gtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGCCCGACGGTCCGTGGTGGTAAAGAATGGTc   <  1:6053265/96‑1 (MQ=255)

                                                                                         gtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTc   >  1:3267133/1‑96 (MQ=255)

                                                                                         gtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGCGGTAAAGAATGGTc   >  2:4081393/1‑96 (MQ=255)

                                                                                          ttATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGtta  <  1:11693437/96‑3 (MQ=255)

                                                                                          ttATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTca  <  2:6190311/96‑1 (MQ=255)

                                                                                          ttATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTca  <  2:10022699/96‑1 (MQ=255)

GGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCA  >  NC_000913/4614145‑4614330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`