BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,823,915	A→G	K21E (AAG→GAG)	srlA →	PTS system, glucitol/sorbitol‑specific IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,823,915	0	A	G	100.0%	1902.7 / NA	487	K21E (AAG→GAG)	srlA	PTS system, glucitol/sorbitol‑specific IIC component
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (260/227); total (260/227)

Only 100 of 513 total aligned reads displayed.

GTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAAAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCAT  >  NC_000913/2823822‑2824007

gTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGagag                                                                                            >  2:14406830/1‑96 (MQ=255)

 taataaAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAgg                                                                                           >  2:9032139/1‑96 (MQ=255)

 taataaAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAgg                                                                                           <  2:411844/96‑1 (MQ=255)

  aataaAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAgga                                                                                          >  2:9692554/1‑95 (MQ=255)

  aataaAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGgt                                                                                          <  2:7690986/96‑1 (MQ=255)

     aaaaCAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGtt                                                                                       <  1:866394/96‑1 (MQ=255)

      aaaCAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGttt                                                                                      <  2:1429139/96‑1 (MQ=255)

       aaCAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTa                                                                                     >  2:9357201/1‑96 (MQ=255)

        aCAATAAATCCTGAAGGAGAGAACAATGATCGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTAc                                                                                    <  2:14098760/96‑1 (MQ=255)

        aCAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGATGTGtttgc                                                                                    <  1:10499825/96‑3 (MQ=255)

         cAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTAcc                                                                                   <  1:14125657/96‑1 (MQ=255)

           aTAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCgg                                                                                 <  1:12340014/96‑1 (MQ=255)

           aTAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCgg                                                                                 <  2:1362476/96‑1 (MQ=255)

            tAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCggg                                                                                <  1:259480/96‑1 (MQ=255)

            tAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCggg                                                                                <  2:8094120/96‑1 (MQ=255)

               aTCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATg                                                                             <  1:4542265/96‑1 (MQ=255)

                 ccTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGt                                                                           <  2:8262429/96‑1 (MQ=255)

                    gAAGGAGAGAACAGTGATAGAAGCCATTACTCATGGTGCAGAGGGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAc                                                                        >  1:10959167/1‑96 (MQ=255)

                    gAAGGAGAGAACAATGATAGAAACCATTACTCATGGAGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAc                                                                        >  1:2515423/1‑96 (MQ=255)

                     aaGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAcc                                                                       >  1:8151904/1‑96 (MQ=255)

                     aaGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAcc                                                                       <  1:2281322/96‑1 (MQ=255)

                      atgagagaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGTTGTTTACCGGGATGGTGACCg                                                                      >  1:11112526/3‑96 (MQ=255)

                      aGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCg                                                                      <  2:12691674/96‑1 (MQ=255)

                      aGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCg                                                                      <  1:4037377/96‑1 (MQ=255)

                          gagaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAt                                                                  >  2:1614489/1‑96 (MQ=255)

                            gaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttc                                                                >  1:686118/1‑96 (MQ=255)

                             aaCAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttct                                                               <  1:13179807/96‑1 (MQ=255)

                             aaCAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttct                                                               <  1:6332618/96‑1 (MQ=255)

                               cAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttcttc                                                             >  1:2012005/1‑96 (MQ=255)

                               cAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttcttc                                                             >  2:7576274/1‑96 (MQ=255)

                               cAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttcttc                                                             <  1:5934681/96‑1 (MQ=255)

                               cAATGATAGAAACCATTACTCATGGTGCAGAGCGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTCTACCGGGATGGTGACCGGCAttcttc                                                             >  2:6276827/1‑96 (MQ=255)

                                     tAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGt                                                       >  2:13484826/1‑96 (MQ=255)

                                     tAGAAACCATTACTCATGGTGCAGAGGGGTTGATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCTGCATTCTTCCGCTGt                                                       >  1:5179507/1‑96 (MQ=255)

                                      aGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGtt                                                      <  1:14349442/96‑1 (MQ=255)

                                       gAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTg                                                     >  1:3358651/1‑96 (MQ=255)

                                          aCCATTAGTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGAtt                                                  <  1:7691652/96‑1 (MQ=255)

                                          aCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGAtt                                                  >  2:2209540/1‑96 (MQ=255)

                                           ccATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTa                                                 >  2:12560752/1‑96 (MQ=255)

                                           ccATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCGGTTGATTa                                                 <  1:12954841/96‑1 (MQ=255)

                                               tACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCct                                             <  1:5686625/96‑1 (MQ=255)

                                               tACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCct                                             <  1:959961/96‑1 (MQ=255)

                                                 cTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgc                                           <  2:606933/96‑1 (MQ=255)

                                                  tCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgct                                          >  1:5499558/1‑96 (MQ=255)

                                                   cATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgctg                                         <  1:2945097/96‑1 (MQ=255)

                                                    aTGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCGTCCGCTGTTGATTAGCCTGCTgg                                        >  1:12524232/1‑96 (MQ=255)

                                                     tGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGt                                       <  2:10693861/96‑1 (MQ=255)

                                                      ggTGCAGGGTGGTTTATCGGGCTGTTCCAAAAGGGCGGGGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTACTTGtt                                      >  1:4239000/1‑96 (MQ=255)

                                                       gTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTa                                     <  2:9568508/96‑1 (MQ=255)

                                                        tGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAt                                    >  2:11059014/1‑96 (MQ=255)

                                                        tGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAt                                    <  1:9032139/96‑1 (MQ=255)

                                                         gaagagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATc                                   >  1:11461434/3‑96 (MQ=255)

                                                          cAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCa                                  >  2:578279/1‑96 (MQ=255)

                                                          cAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGGGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTACCa                                  >  2:6833528/1‑96 (MQ=255)

                                                           agagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCAt                                 <  1:7161830/96‑1 (MQ=255)

                                                            gagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATg                                >  2:5338025/1‑96 (MQ=255)

                                                             agTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGa                               <  1:8432961/96‑1 (MQ=255)

                                                             agTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGa                               <  1:7273802/96‑1 (MQ=255)

                                                             agTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGa                               >  2:13231444/1‑96 (MQ=255)

                                                             agTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTCGATTAGCCTGCTGGTTATCATGa                               >  1:10080026/1‑96 (MQ=255)

                                                               tGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAAc                             >  2:11393161/1‑96 (MQ=255)

                                                                ggTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACg                            >  1:1749355/1‑96 (MQ=255)

                                                                ggTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACg                            <  1:5388620/96‑1 (MQ=255)

                                                                 gTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGc                           >  2:7309078/1‑96 (MQ=255)

                                                                 gTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGc                           >  1:4779121/1‑96 (MQ=255)

                                                                   ttATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCAc                         >  2:2400311/1‑96 (MQ=255)

                                                                   ttATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCAc                         <  2:2825879/96‑1 (MQ=255)

                                                                     aTCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTg                       >  2:11821856/1‑96 (MQ=255)

                                                                     aTCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTg                       <  1:12169289/96‑1 (MQ=255)

                                                                     aTCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTg                       >  1:6542670/1‑96 (MQ=255)

                                                                       cGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGAt                     >  2:1952603/1‑96 (MQ=255)

                                                                         ggCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTa                   <  1:8937587/96‑1 (MQ=255)

                                                                          gCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTaa                  >  1:11533628/1‑96 (MQ=255)

                                                                           cTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAt                 >  2:4566761/1‑96 (MQ=255)

                                                                            tGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAtt                <  1:6133110/96‑1 (MQ=255)

                                                                             gTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAttt               >  1:282798/1‑96 (MQ=255)

                                                                             gTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAttt               <  2:3570061/96‑1 (MQ=255)

                                                                             gTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAttt               <  1:13591018/96‑1 (MQ=255)

                                                                              ttCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAtttt              <  2:5218222/96‑1 (MQ=255)

                                                                              ttCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAtttt              >  2:10462211/1‑96 (MQ=255)

                                                                              ttCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAtttt              <  1:3547170/96‑1 (MQ=255)

                                                                               tCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTa             >  2:8819397/1‑96 (MQ=255)

                                                                               tCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTa             <  1:9357201/96‑1 (MQ=255)

                                                                                 cAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATc           >  2:4448266/1‑96 (MQ=255)

                                                                                 cAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATc           >  2:261283/1‑96 (MQ=255)

                                                                                  aaaaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTacct          <  1:10779255/96‑4 (MQ=255)

                                                                                  aaaaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCg          >  1:13978359/1‑96 (MQ=255)

                                                                                   aaaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCgg         >  2:3338685/1‑96 (MQ=255)

                                                                                   aaaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCgg         >  2:8588455/1‑96 (MQ=255)

                                                                                   aaaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCgg         >  2:7011824/1‑96 (MQ=255)

                                                                                     ggggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTc       >  1:11405163/2‑96 (MQ=255)

                                                                                     aGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTc       >  2:5012390/1‑96 (MQ=255)

                                                                                     aGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTc       <  1:4863769/96‑1 (MQ=255)

                                                                                     aGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTc       <  2:2970680/96‑1 (MQ=255)

                                                                                      gggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCa      <  2:12161673/96‑1 (MQ=255)

                                                                                      gggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCa      >  2:3870591/1‑96 (MQ=255)

                                                                                        gCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGc    <  2:13839291/96‑1 (MQ=255)

                                                                                          ggAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCAt  >  2:6641402/1‑96 (MQ=255)

                                                                                          ggAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCAt  >  1:13405244/1‑96 (MQ=255)

                                                                                          ggAGAGGTGTTTACCGGGATAGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCAt  <  1:3078164/96‑1 (MQ=255)

GTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAAAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCAT  >  NC_000913/2823822‑2824007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`