BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,078,245	C→G	G470G (GGG→GGC)	tktA ←	transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,078,245	0	C	G	99.8%	1546.2 / NA	427	G470G (GGG→GGC)	tktA	transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (211/215); total (211/216)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 499 total aligned reads displayed.

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTA  >  NC_000913/3078152‑3078337

gACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGgcc                                                                                            >  2:11126754/1‑96 (MQ=255)

gACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGgcc                                                                                            >  2:7917398/1‑96 (MQ=255)

gACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGgcc                                                                                            <  1:7329237/96‑1 (MQ=255)

   cgcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGta                                                                                         <  1:8910242/96‑2 (MQ=255)

    gcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                                                        <  1:111698/96‑1 (MQ=255)

      ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                      <  2:1575386/96‑1 (MQ=255)

      ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                      <  1:8059894/96‑1 (MQ=255)

      ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                      <  1:7410894/96‑1 (MQ=255)

         ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCTGCTGGTGATAAGGGCCGTCTTCGcc                                                                                   >  1:12175864/1‑96 (MQ=255)

          tCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                                                  <  2:6286908/96‑1 (MQ=255)

          tCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                                                  >  1:396257/1‑96 (MQ=255)

            aaCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAg                                                                                >  2:8669614/1‑96 (MQ=255)

            aaCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGGTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGGCGGGCCGTCTTCGCCCAg                                                                                >  2:1794886/1‑96 (MQ=255)

             aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGTCGGCTTCGCCCAGa                                                                               >  1:5834164/1‑96 (MQ=255)

             aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGa                                                                               >  1:11244756/1‑96 (MQ=255)

              ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                                              <  1:3013363/96‑1 (MQ=255)

              ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                                              <  2:9349118/96‑1 (MQ=255)

               cTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAcc                                                                             <  1:8371526/96‑1 (MQ=255)

                tGGTCACACGGACGCCATGTAGACATGTCCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCg                                                                            <  2:960568/96‑1 (MQ=255)

                 ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                           <  2:12075822/96‑1 (MQ=255)

                  gTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                          >  2:2737606/1‑96 (MQ=255)

                  gTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                          <  2:5523362/96‑1 (MQ=255)

                  gTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                          <  2:6942289/96‑1 (MQ=255)

                   tCACCCGGACCCCATGTAGCCTTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                         <  1:4360570/96‑1 (MQ=255)

                   tCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                         <  2:14290378/96‑1 (MQ=255)

                   tCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                         <  2:4717659/96‑1 (MQ=255)

                    cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCCATgg                                                                        <  1:12604321/96‑1 (MQ=255)

                      cacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAg                                                                      >  2:9784319/1‑96 (MQ=255)

                       acGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGt                                                                     <  1:5108717/96‑1 (MQ=255)

                        gggtcGCCATGTAGACATGTTCGGGGTTACGCGCAGGGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTc                                                                    <  1:4691122/92‑1 (MQ=255)

                        cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTc                                                                    >  1:623442/1‑96 (MQ=255)

                        cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAACGATGGAggc                                                                    >  1:1748688/1‑94 (MQ=255)

                          gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                                                  >  1:11282558/1‑96 (MQ=255)

                           aCGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                                                 <  2:10874140/96‑1 (MQ=255)

                            cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGCCGTgg                                                                >  2:11521762/1‑96 (MQ=255)

                             gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                               <  1:2688750/96‑1 (MQ=255)

                              cgaTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                                              >  1:11786253/3‑96 (MQ=255)

                              ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGGTGGAGTCGCgggg                                                              >  2:5360946/1‑95 (MQ=255)

                                aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                                            <  1:8653422/96‑1 (MQ=255)

                                 tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTa                                                           >  2:3618954/1‑96 (MQ=255)

                                  gTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaa                                                          >  2:659588/1‑96 (MQ=255)

                                  gTAGACATATTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaa                                                          >  1:12943636/1‑96 (MQ=255)

                                   tAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaaa                                                         <  2:10589653/96‑1 (MQ=255)

                                    aGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTCTAAAc                                                        >  2:12742425/1‑96 (MQ=255)

                                     gACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGGCGTGGGTGTAAAcc                                                       >  2:9471563/1‑96 (MQ=255)

                                     gACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCCCAACCGGCGGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                                                       <  1:9711396/96‑1 (MQ=255)

                                       cATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGCGTAAAccct                                                     >  1:1382180/1‑94 (MQ=255)

                                          gTTCGGGGTTCCGCGCAGAGAAGCGCCCTGCTCACCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAc                                                  <  1:14225125/96‑1 (MQ=255)

                                           ttCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAcc                                                 >  1:13542201/1‑96 (MQ=255)

                                            tCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                                <  2:4216972/96‑1 (MQ=255)

                                            tCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                                <  1:13220610/96‑1 (MQ=255)

                                            tCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                                <  1:489220/96‑1 (MQ=255)

                                            tCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                                <  1:7013117/96‑1 (MQ=255)

                                             cGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTg                                               <  1:10869169/96‑1 (MQ=255)

                                             cGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTg                                               >  2:2496836/1‑96 (MQ=255)

                                              ggggTTACGCGCAGAGAAGCGACATGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                                              <  1:10366308/96‑1 (MQ=255)

                                               gggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                             <  1:10000650/96‑1 (MQ=255)

                                               gggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                             <  2:807592/96‑1 (MQ=255)

                                               gggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                             <  2:13554821/96‑1 (MQ=255)

                                                   tACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTg                                         >  1:11545695/1‑96 (MQ=255)

                                                   tACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGGCGGGCCGTCTTCGCCCAGACCGGTTGAGTCGAGGGTGTAAACCATCACCTGACGCTg                                         >  2:11832241/1‑96 (MQ=255)

                                                      gcgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt                                      <  1:7618026/96‑1 (MQ=255)

                                                       cgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtc                                     >  2:3971088/1‑96 (MQ=255)

                                                        gcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca                                    <  2:3070999/96‑1 (MQ=255)

                                                         cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcat                                   <  2:5098016/96‑1 (MQ=255)

                                                         cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcat                                   <  2:2191452/96‑1 (MQ=255)

                                                         cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGGTtcat                                   >  2:827117/1‑96 (MQ=255)

                                                          agagAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                                  <  1:659588/96‑1 (MQ=255)

                                                          agagAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                                  <  2:4946363/96‑1 (MQ=255)

                                                          agagAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                                  <  2:4462976/96‑1 (MQ=255)

                                                           gagaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca                                 <  2:13175619/96‑1 (MQ=255)

                                                           gagaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca                                 <  2:9733014/96‑1 (MQ=255)

                                                           gagaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca                                 >  2:7442307/1‑96 (MQ=255)

                                                            agaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAg                                >  2:14047312/1‑96 (MQ=255)

                                                             gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc                               <  1:7950245/96‑1 (MQ=255)

                                                             gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGGAAACCATCACCTGACGCTGTTTCATCAgc                               >  2:11898366/1‑96 (MQ=255)

                                                             gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGGCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc                               >  1:9393648/1‑96 (MQ=255)

                                                                 ctaCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAg                           >  1:10558250/3‑96 (MQ=255)

                                                                 cGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAg                           >  2:10129445/1‑96 (MQ=255)

                                                                  gACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGc                          <  1:4181439/96‑1 (MQ=255)

                                                                  gACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGc                          >  2:5216693/1‑96 (MQ=255)

                                                                      tGCTCAACCGGCTGGTGAGTCGGTCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCTCCTGACGCTGTTTCATCAGCGCAGCCAta                      <  2:7724651/96‑1 (MQ=255)

                                                                      tGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAta                      >  1:5346827/1‑96 (MQ=255)

                                                                       gCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtac                     <  2:11274848/96‑1 (MQ=255)

                                                                       gCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtac                     <  2:1938957/96‑1 (MQ=255)

                                                                         tCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgt                   <  2:7062823/96‑1 (MQ=255)

                                                                            aCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtacg                <  2:8989054/96‑1 (MQ=255)

                                                                            aCCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtacg                >  1:1520511/1‑96 (MQ=255)

                                                                               ggCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCg             >  2:12468242/1‑96 (MQ=255)

                                                                               ggCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCg             <  2:3184839/96‑1 (MQ=255)

                                                                               ggCTGGTGAGGCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCg             >  2:5842260/1‑96 (MQ=255)

                                                                                  tGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTa          >  1:3215851/1‑96 (MQ=255)

                                                                                   ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAACCCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTAc         <  1:3710132/96‑1 (MQ=255)

                                                                                   ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTAc         <  1:14047312/96‑1 (MQ=255)

                                                                                   ggTGAGGCGGTCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTCTTTCATCAGCGCAGCCATACGGACGGCGTTAc         <  2:3311383/96‑1 (MQ=255)

                                                                                    gTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACg        >  1:3402952/1‑96 (MQ=255)

                                                                                     tGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGt       >  2:13716103/1‑96 (MQ=255)

                                                                                      gAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTg      <  1:9935285/96‑1 (MQ=255)

                                                                                        gTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCg    >  1:216994/1‑96 (MQ=255)

                                                                                          cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGGCGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTa  >  2:3831111/1‑96 (MQ=255)

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTA  >  NC_000913/3078152‑3078337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`