BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,130,334	A→G	V30V (GTT→GTC)	b2983 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,130,334	0	A	G	99.8%	1574.8 / NA	395	V30V (GTT→GTC)	b2983	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (174/220); minor base T (0/1); total (174/221)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 468 total aligned reads displayed.

GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTC  >  NC_000913/3130241‑3130426

 tACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGGGCCCacgaca                                                                                           >  2:7146893/1‑96 (MQ=255)

  aCGATTGCACAATCACCAGTACGCCAAACATGGCGGGGGTCATCCCTTTTCCGGCAAACGAGAGCGCCAACAGACCCAGGAAGGCGCTCACGACAt                                                                                          >  1:3764250/1‑96 (MQ=255)

  aCGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAt                                                                                          <  2:11328380/96‑1 (MQ=255)

  aCGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCCTTACCGGCACACCAGAGCGCCAACAAACCCAGCAAGGCGCTCacgacaa                                                                                          >  2:4480523/1‑95 (MQ=255)

   cGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAtt                                                                                         <  2:5836750/96‑1 (MQ=255)

    gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTa                                                                                        >  1:12155479/1‑96 (MQ=255)

     aTTGCACAATCACCAGTACGCCAAACATGGCGGGCGGCATCCCCTTACCGGCACACCAGAGCGCCAACAGACCCAGCAAGGGGCTGACGACATTAc                                                                                       >  2:13205627/1‑96 (MQ=255)

          agaaTCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACTACATTACCGGAg                                                                                  >  1:10542821/3‑96 (MQ=255)

          acaATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAg                                                                                  <  2:2277494/96‑1 (MQ=255)

          acaATCACCAGTACGCCAAACATGGCGGGCGGCCTCCCTTTACCGGCACCCGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAg                                                                                  >  2:2634143/1‑96 (MQ=255)

           caATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACACTACTGGAGc                                                                                 >  1:5135125/1‑96 (MQ=255)

            aaTCACCAGTACGCCACACATGGCGGGCGTCATCCCTTTACCGGCACCCGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGcc                                                                                <  1:1361456/96‑1 (MQ=255)

            aaTCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTAACGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTTGAGcc                                                                                >  1:7459958/1‑96 (MQ=255)

             aTCACAAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCt                                                                               >  1:8101768/1‑96 (MQ=255)

               ccccAGTCCGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTaa                                                                             <  2:5524579/94‑1 (MQ=255)

                aCCAGTACGCCAAACATGGCGGGCGCCATCCCTTTACCGGCACACGAGAGCGCCCACAGAACCACCAAAGCGCTCACGACATTAATGGAGCCTAAc                                                                            >  2:636587/1‑96 (MQ=255)

                    gTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAgg                                                                        <  2:2175032/96‑1 (MQ=255)

                    gTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAgg                                                                        <  2:1815564/96‑1 (MQ=255)

                     tACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgc                                                                       <  2:8709105/96‑1 (MQ=255)

                     tACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgc                                                                       <  2:12667767/96‑1 (MQ=255)

                      aCGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcg                                                                      <  2:10940462/96‑1 (MQ=255)

                       cGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcgc                                                                     >  1:10939254/1‑96 (MQ=255)

                       cGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcgc                                                                     <  1:14037100/96‑1 (MQ=255)

                        gCCCAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCt                                                                    <  1:4229476/96‑1 (MQ=255)

                          cAAACATGGCGGGCGTCATCCCTTTACCGGCCCACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAt                                                                  <  2:13461319/96‑1 (MQ=255)

                            agtaTGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCCCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTg                                                                <  1:10435408/93‑1 (MQ=255)

                            aaCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCACGCGCTATTg                                                                >  1:14346410/1‑96 (MQ=255)

                             aCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGc                                                               >  1:7720768/1‑96 (MQ=255)

                              cATGGCGGCCGTCACCCCTTTCCCGGCACACGAGAGCGCCAAAAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCg                                                              <  2:3753752/96‑1 (MQ=255)

                                tGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaa                                                            >  2:11187024/1‑96 (MQ=255)

                                 ggcgggcgTCATCCTGTGACCGGCACAGGAGCCCGCCAACAGACCCAGCAGGCCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaat                                                           <  1:2634143/96‑1 (MQ=255)

                                 ggcgggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaat                                                           <  1:13485406/96‑1 (MQ=255)

                                 ggcgggcgTCATCCCTTTACCGGCACACGAGACCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaat                                                           <  2:2957367/96‑1 (MQ=255)

                                   cgggcgTCATCCCTTTACCGGCCCACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataa                                                         <  2:8860267/96‑1 (MQ=255)

                                    gggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                                        <  1:4335864/96‑1 (MQ=255)

                                     ggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATg                                                       <  1:12760186/96‑1 (MQ=255)

                                     ggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATg                                                       <  2:1582073/96‑1 (MQ=255)

                                     ggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATg                                                       <  1:1536959/96‑1 (MQ=255)

                                     gccgTCACCCCTTTCCCGGAACACAAGAGCGCCAACAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATg                                                       <  1:4992509/94‑1 (MQ=255)

                                      gcgTGATCCCTTTACCGGGACACGAGAGCGCCAACAGACACAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                                      >  1:10147415/1‑96 (MQ=255)

                                      gcgTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                                      <  2:13813384/96‑1 (MQ=255)

                                      gcgTCATCCCTTTACCGGCCCACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataatcg                                                      <  1:5689718/96‑3 (MQ=255)

                                      gcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCACCAAGGCGCACACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                                      <  2:3319325/96‑1 (MQ=255)

                                        gccATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTg                                                    <  1:12906006/94‑1 (MQ=255)

                                        gTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTg                                                    <  2:13674201/96‑1 (MQ=255)

                                        gTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTg                                                    >  2:11622801/1‑96 (MQ=255)

                                         tCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                                                   >  1:13870259/1‑96 (MQ=255)

                                          cATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGcgcg                                                  >  1:14243275/1‑96 (MQ=255)

                                            tCCCTTTCCCGGCCCACGAGAGCGCCAACAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGNGaa                                                <  2:522210/96‑1 (MQ=255)

                                            tCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaa                                                <  2:6115321/96‑1 (MQ=255)

                                              ccTTTACCGCCCCACGAGAGCGCAAACAGACCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                              <  2:5010252/96‑1 (MQ=255)

                                               cTTTCCCGGCCCACGAGAGCGCAAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcg                                             <  2:5631131/96‑1 (MQ=255)

                                               cTTTACCGGCACAGGAGAGCGCCAACAGCCCAAGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcg                                             <  2:9557473/96‑1 (MQ=255)

                                               cTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAct                                             <  1:11268793/96‑2 (MQ=255)

                                                 ttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcgcg                                           >  1:185432/1‑96 (MQ=255)

                                                  tACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACgcgc                                          >  2:732357/1‑96 (MQ=255)

                                                   aCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGcc                                         >  1:3542783/1‑96 (MQ=255)

                                                     cGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCAt                                       >  2:7716462/1‑96 (MQ=255)

                                                     cGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCAt                                       >  1:2769571/1‑96 (MQ=255)

                                                      ggCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATc                                      >  2:8336842/1‑96 (MQ=255)

                                                       gCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCt                                     >  2:11041400/1‑96 (MQ=255)

                                                        cacaGGAGACCCCAAACAGCCCCAGCAGGCCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTg                                    <  1:7096311/96‑1 (MQ=255)

                                                          cacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCa                                  >  2:10009426/1‑96 (MQ=255)

                                                           acGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaa                                 >  2:8038705/1‑96 (MQ=255)

                                                           acGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCATAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaa                                 >  2:10314173/1‑96 (MQ=255)

                                                            cGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaaa                                >  1:885323/1‑96 (MQ=255)

                                                              agagCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAAcc                              <  2:4640936/96‑1 (MQ=255)

                                                                 gcgcCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                           <  2:11757987/96‑1 (MQ=255)

                                                                 gcgcAACCAGCCCCAGAAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                           <  1:8484201/96‑1 (MQ=255)

                                                                  cgcCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATg                          <  2:710242/96‑1 (MQ=255)

                                                                  caccaccaGACACAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATg                          <  2:3542783/94‑1 (MQ=255)

                                                                   gcaaaCAGACCCAGAAAGGCGTTAACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGt                         <  1:12544542/93‑1 (MQ=255)

                                                                   gcCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCCAACCAATGt                         >  1:2276288/1‑96 (MQ=255)

                                                                   gcCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGt                         >  2:6622277/1‑96 (MQ=255)

                                                                   gcCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGt                         >  1:10978909/1‑96 (MQ=255)

                                                                     cAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGttt                       <  1:6387277/96‑1 (MQ=255)

                                                                      aaCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTg                      <  2:5325124/96‑1 (MQ=255)

                                                                       aCAGGCCCAGGAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCCAACCAATGTTTGa                     <  2:14224696/96‑1 (MQ=255)

                                                                        cAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAt                    >  2:4150738/1‑96 (MQ=255)

                                                                         aGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATg                   >  1:2931680/1‑96 (MQ=255)

                                                                         aGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCAGCTGCAAACCAATGTTTGATg                   >  1:6840596/1‑96 (MQ=255)

                                                                            cccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGtca                <  1:11493845/96‑3 (MQ=255)

                                                                              cAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGGTGTTaaa              >  1:14415641/1‑96 (MQ=255)

                                                                              cAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaa              >  2:6866061/1‑96 (MQ=255)

                                                                               aGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaaa             <  1:13080264/96‑1 (MQ=255)

                                                                                 cAAGGCGCCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAcc           <  2:11990812/96‑1 (MQ=255)

                                                                                  aaGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAccc          >  2:4849993/1‑96 (MQ=255)

                                                                                  aaGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAccc          <  1:11622801/96‑1 (MQ=255)

                                                                                   aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCg         >  2:10536477/1‑96 (MQ=255)

                                                                                   aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCg         <  1:14154784/96‑1 (MQ=255)

                                                                                    ggCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGc        >  1:8303878/1‑96 (MQ=255)

                                                                                    ggCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGc        <  2:12399697/96‑1 (MQ=255)

                                                                                     gcgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATTGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTGAAAACCCGca       >  1:3300732/1‑95 (MQ=255)

                                                                                      cgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCa      >  1:9371657/1‑96 (MQ=255)

                                                                                      cgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCa      <  1:6104453/96‑1 (MQ=255)

                                                                                       gcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCaa     >  1:9713965/1‑96 (MQ=255)

                                                                                        cTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAt    >  1:3934208/1‑96 (MQ=255)

                                                                                        cTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAt    >  2:9267729/1‑96 (MQ=255)

                                                                                         tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAtt   <  2:10127323/96‑1 (MQ=255)

                                                                                         tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAtt   <  1:4537296/96‑1 (MQ=255)

GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTC  >  NC_000913/3130241‑3130426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`