Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,734,264 2 bp→GC coding (542‑543/825 nt) bax ← putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,734,2640CG99.8% 2263.7 / NA 563S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (275/287);  minor base T (1/0);  total (276/287)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,734,2650GC100.0% 2247.0 / NA 563S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (276/287);  total (276/287)

Only 100 of 576 total aligned reads displayed.

GGTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACTG  >  NC_000913/3734170‑3734354
                                                                                              ||                                                                                         
ggTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTCCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATcc                                                                                           <  1:8995372/96‑2 (MQ=255)
    aGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCAttt                                                                                       <  2:12832088/96‑1 (MQ=255)
    aGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCAttt                                                                                       <  2:5852288/96‑1 (MQ=255)
      tGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCa                                                                                     <  2:3006727/96‑1 (MQ=255)
      tGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCa                                                                                     <  2:3499821/96‑1 (MQ=255)
       gACATAGGCGCTCACCGATTCTTTGACAGAACTAACCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAt                                                                                    <  1:9203210/96‑1 (MQ=255)
       gACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAt                                                                                    <  1:11002848/96‑1 (MQ=255)
        aCATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATg                                                                                   >  1:10855737/1‑96 (MQ=255)
         cATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGc                                                                                  <  1:11246100/96‑1 (MQ=255)
         cATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGc                                                                                  <  2:3898637/96‑1 (MQ=255)
          aTAGGCGCTCATCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGcc                                                                                 <  1:5178171/96‑1 (MQ=255)
          aTAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTACATGcc                                                                                 <  2:13691040/96‑1 (MQ=255)
           tAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCg                                                                                <  2:10650653/96‑1 (MQ=255)
           tAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCg                                                                                <  1:12175516/96‑1 (MQ=255)
            aGGCACTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCACATTGGTACAACGTCCTTTCATGCATTTCATGCCGa                                                                               <  1:9471726/96‑1 (MQ=255)
              gcgcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAc                                                                             >  1:1901595/1‑96 (MQ=255)
                gcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAg                                                                           >  2:14107814/1‑96 (MQ=255)
                gcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAg                                                                           >  1:12439157/1‑96 (MQ=255)
                gcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAg                                                                           >  2:12465586/1‑96 (MQ=255)
                    aCCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                       >  1:1625977/1‑96 (MQ=255)
                      cGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgtt                                                                     >  2:9526755/1‑96 (MQ=255)
                       gATTCTTTTACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttg                                                                    >  2:5429770/1‑96 (MQ=255)
                       gATTCCTTGACAGAACTTAACTGTGAGTACCCTTTCCCTTTACCTGGCGCATTGGTCCACCGTCCTCTCATGCATTTCATGCCGAACAGgttgttg                                                                    <  2:1888971/96‑1 (MQ=255)
                        aTTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgt                                                                   <  2:7100232/96‑1 (MQ=255)
                         ttCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgtt                                                                  <  2:13109184/96‑1 (MQ=255)
                           cTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCCTTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttat                                                                <  1:10046142/96‑3 (MQ=255)
                            tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                               <  1:5028086/96‑1 (MQ=255)
                            tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                               <  2:6761475/96‑1 (MQ=255)
                            tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                               <  2:7865137/96‑1 (MQ=255)
                            tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                               <  2:8686408/96‑1 (MQ=255)
                             ttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGttgttgttgttg                                                              >  1:6069674/1‑96 (MQ=255)
                              tGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgc                                                             >  2:4796296/1‑96 (MQ=255)
                              tGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGGTGTTGTTGTTgc                                                             >  1:4398906/1‑96 (MQ=255)
                               gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcg                                                            >  2:3651909/1‑96 (MQ=255)
                                aCAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                           >  2:13498814/1‑96 (MQ=255)
                                aCAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                           <  2:2845231/96‑1 (MQ=255)
                                aCAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                           <  2:12152077/96‑1 (MQ=255)
                                 cAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgcg                                                          <  2:9780189/96‑1 (MQ=255)
                                  aGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgcgc                                                         >  1:13313305/1‑96 (MQ=255)
                                  aGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgcgc                                                         >  1:6343946/1‑96 (MQ=255)
                                  aGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgcgc                                                         <  2:4264197/96‑1 (MQ=255)
                                   gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGcc                                                        <  2:13932477/96‑1 (MQ=255)
                                    aaCTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGATTGTTGTTGTTGCGCGCCa                                                       <  2:12365092/96‑1 (MQ=255)
                                     aCTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAg                                                      <  1:14386895/96‑1 (MQ=255)
                                     aCTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAg                                                      >  1:14244401/1‑96 (MQ=255)
                                      cTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGc                                                     >  2:8961582/1‑96 (MQ=255)
                                       tAAACTGTGAGTACACTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCt                                                    >  1:5673881/1‑96 (MQ=255)
                                        aaaCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCtt                                                   >  2:2627640/1‑96 (MQ=255)
                                         aaCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTc                                                  >  2:4975405/1‑96 (MQ=255)
                                         aaCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTc                                                  >  1:4503772/1‑96 (MQ=255)
                                          aatgtgAGTACCCTTTCACTTAACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCttag                                                 >  1:12870032/3‑94 (MQ=255)
                                          aCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCg                                                 <  1:10387007/96‑1 (MQ=255)
                                           cTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                                <  2:11790356/96‑1 (MQ=255)
                                            tgtgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAc                                               <  1:13593644/96‑1 (MQ=255)
                                            tgtgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAc                                               >  1:8204059/1‑96 (MQ=255)
                                               gAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGtt                                            >  2:8283460/1‑96 (MQ=255)
                                                 gtccccTTTCACTTTTCCTGGCGCATTTGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcc                                          <  1:11563933/93‑1 (MQ=255)
                                                  tACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcct                                         <  1:11416529/96‑2 (MQ=255)
                                                   aCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAGCAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                        >  1:2272364/1‑96 (MQ=255)
                                                   aCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                        >  2:218239/1‑96 (MQ=255)
                                                    cccTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCa                                       <  1:399297/96‑1 (MQ=255)
                                                      cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAc                                     >  1:6588046/1‑96 (MQ=255)
                                                      cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGCTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAc                                     >  2:12221627/1‑96 (MQ=255)
                                                       tttCCCTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAccc                                    <  1:346999/96‑1 (MQ=255)
                                                       tttCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAcc                                    >  2:9167862/1‑96 (MQ=255)
                                                        ttCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCg                                   <  1:12575303/96‑1 (MQ=255)
                                                        ttCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCg                                   <  2:13291664/96‑1 (MQ=255)
                                                           tcTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCtt                                <  1:3209177/95‑1 (MQ=255)
                                                            cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCttt                               >  1:285053/1‑96 (MQ=255)
                                                              ttACCTGGCGCATTGGTCCAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCGCCAACCGCTTTcc                             <  1:13843270/96‑2 (MQ=255)
                                                              ttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTct                             <  2:11178030/96‑1 (MQ=255)
                                                               tACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctg                            >  2:14336563/1‑96 (MQ=255)
                                                               tACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctg                            <  2:5591419/96‑1 (MQ=255)
                                                               tACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctg                            <  2:10869812/96‑1 (MQ=255)
                                                               tACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCACCACCGCTTTctg                            <  1:13423160/96‑1 (MQ=255)
                                                                aCCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCCACCGCTTTctgc                           >  1:3656877/1‑96 (MQ=255)
                                                                aCCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgc                           >  1:799315/1‑96 (MQ=255)
                                                                 ccTGGCGCATTGGTTCAACGTCCTTTTATTCATTTCATGCCGGACAGGGTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgct                          <  1:5548150/96‑1 (MQ=255)
                                                                 ccTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgct                          <  2:12665327/96‑1 (MQ=255)
                                                                  cTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgctg                         <  1:8383037/96‑1 (MQ=255)
                                                                   tGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCtgctgc                        >  1:9783123/1‑96 (MQ=255)
                                                                     gcgcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAg                      <  2:8009887/96‑1 (MQ=255)
                                                                      cgcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGc                     <  2:6371250/96‑1 (MQ=255)
                                                                       gcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGcc                    <  2:6017866/96‑1 (MQ=255)
                                                                        cATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCa                   >  1:1459636/1‑96 (MQ=255)
                                                                        cATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCa                   >  1:6712667/1‑96 (MQ=255)
                                                                         aTTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAt                  >  2:11569781/1‑96 (MQ=255)
                                                                          ttGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtc                 <  2:11105494/96‑1 (MQ=255)
                                                                             gTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGGTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtc              >  2:7589637/1‑96 (MQ=255)
                                                                              tACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtcg             <  1:14159279/96‑1 (MQ=255)
                                                                                cAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGcc           >  2:1752590/1‑96 (MQ=255)
                                                                                 aaCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGcca          >  1:8606739/1‑96 (MQ=255)
                                                                                 aaCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGcca          >  2:7761975/1‑96 (MQ=255)
                                                                                 aaCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGGTCCCCAACCGCTTTCTGCTGCAGCCATCGGAGcca          >  1:7524157/1‑96 (MQ=255)
                                                                                    gTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca       <  1:11944316/96‑1 (MQ=255)
                                                                                    gTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca       >  2:10069791/1‑96 (MQ=255)
                                                                                    gTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca       <  1:2570027/96‑1 (MQ=255)
                                                                                     tCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCAt      >  2:3223396/1‑96 (MQ=255)
                                                                                        tttCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACt   <  2:1516984/96‑1 (MQ=255)
                                                                                        tttCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACt   <  1:7164579/96‑1 (MQ=255)
                                                                                              ||                                                                                         
GGTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACTG  >  NC_000913/3734170‑3734354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑