BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,421,120	Δ1 bp	noncoding (60/121 nt)	rrfF ←	5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,421,119	0	G	.	93.8%	225.6 / 15.6	96	noncoding (61/121 nt)	rrfF	5S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base G (5/1); new base . (42/48); total (47/49)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 104 total aligned reads displayed.

CAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAA  >  NC_000913/3421027‑3421207

  gTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgtt                                                                                        <  1:4950646/93‑3 (MQ=38)

   tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                       >  2:5893096/1‑93 (MQ=255)

   tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                       >  1:408014/1‑93 (MQ=255)

   tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                       >  2:2867864/1‑93 (MQ=255)

   tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                       >  2:3430636/1‑93 (MQ=37)

   tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                       >  2:2867865/1‑93 (MQ=255)

    cTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGtttc                                                                                      <  2:2012802/93‑2 (MQ=25)

     ttCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGtttcc                                                                                     >  2:3112542/1‑91 (MQ=255)

     ttCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCa                                                                                    >  2:797769/1‑93 (MQ=255)

     ttCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCa                                                                                    >  1:1829440/1‑93 (MQ=255)

     ttCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCa                                                                                    <  2:4485987/93‑1 (MQ=19)

      tCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCAc                                                                                   <  2:4501259/93‑1 (MQ=16)

       ccGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACt                                                                                  >  1:5996782/1‑93 (MQ=12)

       ccGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACt                                                                                  <  2:2714690/93‑1 (MQ=12)

        cGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCGCACTACCATCGGCGCTACGGC‑GTTTCACtt                                                                                 <  2:1752458/93‑1 (MQ=255)

        cGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACtt                                                                                 <  2:2083855/93‑1 (MQ=9)

        cGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACtt                                                                                 <  2:2176270/93‑1 (MQ=9)

        cGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACtt                                                                                 <  1:411455/93‑1 (MQ=9)

         gACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTc                                                                                >  2:2548554/1‑93 (MQ=16)

         gACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTc                                                                                <  2:2274461/93‑1 (MQ=9)

         gACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTc                                                                                >  1:5273180/1‑93 (MQ=12)

          aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCt                                                                               >  2:1391555/1‑93 (MQ=12)

          aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCt                                                                               >  2:1714696/1‑93 (MQ=12)

          aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCt                                                                               >  2:6123840/1‑93 (MQ=16)

          aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCt                                                                               <  1:6135948/93‑1 (MQ=9)

           cTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTg                                                                              >  1:559186/1‑93 (MQ=12)

           cTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTg                                                                              >  1:2913233/1‑93 (MQ=12)

           cTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTg                                                                              >  2:1544780/1‑93 (MQ=12)

            tGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGa                                                                             >  1:2689591/1‑93 (MQ=12)

            tGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGa                                                                             >  1:4021925/1‑93 (MQ=16)

            tGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGa                                                                             >  2:4097545/1‑93 (MQ=16)

             gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                            >  2:692026/1‑93 (MQ=16)

             gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                            >  2:3645489/1‑93 (MQ=16)

             gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                            >  1:4949250/1‑93 (MQ=16)

             gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                            >  2:2307391/1‑93 (MQ=16)

             gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                            >  2:250523/1‑93 (MQ=16)

             gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                            >  2:276636/1‑93 (MQ=9)

              aGCTTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGt                                                                           <  1:4224371/93‑1 (MQ=9)

              aGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGt                                                                           >  1:1395618/1‑93 (MQ=12)

              aGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGt                                                                           >  1:4365982/1‑93 (MQ=16)

              aGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGt                                                                           >  1:3705910/1‑93 (MQ=9)

               gCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGtt                                                                          >  1:3607112/1‑93 (MQ=16)

               gCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGtt                                                                          >  1:2018003/1‑93 (MQ=12)

               gCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGtt                                                                          >  2:5826904/1‑93 (MQ=16)

                ccTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTc                                                                         <  2:1980721/93‑1 (MQ=9)

                ccTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTc                                                                         <  1:2988385/93‑1 (MQ=9)

                ccTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTc                                                                         <  1:2908255/93‑1 (MQ=9)

                ccTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTc                                                                         <  2:3366222/93‑1 (MQ=9)

                cattttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTc                                                                         <  2:5464737/91‑1 (MQ=9)

                 cTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCg                                                                        >  2:5115990/1‑93 (MQ=9)

                 cTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCg                                                                        >  1:3299544/1‑93 (MQ=12)

                 cTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCg                                                                        >  1:2349527/1‑93 (MQ=12)

                  ttttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGTCCCCACACTACCATCGGCGCTACGGC‑GTATCACTTCTGAGATCgg                                                                       >  1:178586/1‑93 (MQ=11)

                  ttttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCgg                                                                       >  2:3234401/1‑93 (MQ=9)

                    ttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCa                                                                     <  1:3058714/93‑1 (MQ=0)

                    ttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCa                                                                     <  2:2275146/93‑1 (MQ=0)

                    ttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCa                                                                     <  2:4192372/93‑1 (MQ=0)

                    ttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCa                                                                     <  1:2712120/93‑1 (MQ=0)

                    ttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCa                                                                     <  2:4192373/93‑1 (MQ=0)

                             ttGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgt                                                                                         >  1:1171869/1‑64 (MQ=25)

                                                                   cccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCttt                      <  1:1824438/93‑1 (MQ=0)

                                                                    ccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCtttt                     <  2:5036707/93‑1 (MQ=12)

                                                                     cacaCTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                    >  1:5872372/1‑93 (MQ=12)

                                                                     cacaCTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                    <  2:1643811/93‑1 (MQ=12)

                                                                     cacaCTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGCGGGACCACCACGCTACTGCCCCCAGACAAGTTCTTTTc                    >  2:146336/1‑93 (MQ=11)

                                                                      acacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                   <  2:5874465/93‑1 (MQ=19)

                                                                       cccTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTa                  <  2:5673604/91‑1 (MQ=255)

                                                                       cacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTa                  <  1:5693122/93‑1 (MQ=19)

                                                                        acTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTaa                 <  2:2028005/93‑1 (MQ=255)

                                                                         cTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAAt                <  2:5454605/93‑1 (MQ=255)

                                                                          tACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATc               <  1:4519073/93‑1 (MQ=255)

                                                                          tACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATc               <  1:4704315/93‑1 (MQ=255)

                                                                          tACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATc               >  2:5710679/1‑93 (MQ=255)

                                                                          caCCATCGGCGCTACGGC‑TTTTCACTTCTGAGTGCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATc               <  1:4519072/92‑1 (MQ=14)

                                                                           aCCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCt              <  1:6124436/93‑1 (MQ=255)

                                                                           aCCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCt              <  2:2982626/93‑1 (MQ=255)

                                                                           aCCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCt              <  2:5402610/93‑1 (MQ=255)

                                                                              aTCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGcc           <  1:2850733/93‑1 (MQ=255)

                                                                              aTCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGcc           <  2:3432789/93‑1 (MQ=255)

                                                                              aTCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGcc           <  2:2634274/93‑1 (MQ=255)

                                                                               tCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTATTCTGCCg          >  1:4597578/1‑93 (MQ=255)

                                                                               tCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCg          >  2:2226119/1‑93 (MQ=255)

                                                                               tCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCg          >  1:738298/1‑93 (MQ=255)

                                                                                cGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGa         <  2:584840/93‑1 (MQ=255)

                                                                                 ggCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGaa        >  2:4295971/1‑93 (MQ=255)

                                                                                  gcgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAc       <  1:3696169/93‑1 (MQ=255)

                                                                                  gcgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAc       <  2:5169048/93‑1 (MQ=255)

                                                                                   cgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACt      <  2:4007659/93‑1 (MQ=255)

                                                                                    gcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAcct     >  2:4368269/1‑91 (MQ=255)

                                                                                    gcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACtt     >  2:421083/1‑93 (MQ=255)

                                                                                    gcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACtt     <  1:4530088/93‑1 (MQ=255)

                                                                                    gcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACtt     >  2:2438590/1‑93 (MQ=255)

                                                                                     cTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt    <  1:4640562/93‑1 (MQ=255)

                                                                                     cTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt    >  2:2971020/1‑93 (MQ=255)

                                                                                     cTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt    >  1:422670/1‑93 (MQ=255)

                                                                                     cTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt    <  1:1676250/93‑1 (MQ=255)

                                                                                     cTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGGCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTTTTCTTATCTGCCGAACctt    >  1:2974947/1‑93 (MQ=11)

                                                                                      tACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTa   <  1:4590755/93‑1 (MQ=255)

                                                                                      tACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTa   <  1:5917309/93‑1 (MQ=255)

                                                                                       aCGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTaa  <  2:2831155/93‑1 (MQ=255)

CAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAA  >  NC_000913/3421027‑3421207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`