BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,061,961	Δ1 bp	coding (1386/1458 nt)	b3876 ←	putative permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,061,959	0	C	.	98.9%	848.3 / ‑0.4	185	coding (1388/1458 nt)	b3876	putative permease
Reads supporting (aligned to +/- strand): ref base C (2/0); new base . (110/73); total (112/73)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

Only 100 of 202 total aligned reads displayed.

TTTTTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTA  >  NC_000913/4061867‑4062047

tttttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGc                                                                                          >  2:2669080/1‑93 (MQ=255)

   ttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGccgt                                                                                       >  1:2806566/1‑91 (MQ=255)

     cATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGccgttt                                                                                     >  1:4041922/1‑89 (MQ=255)

     cATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGccgttt                                                                                     >  2:5005786/1‑89 (MQ=255)

      ataataAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGtttt                                                                                   >  2:2519333/1‑93 (MQ=255)

      ataataAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTAGTGACATTTATAACGTTACGGAAG‑CCGtttt                                                                                   >  1:3928808/1‑93 (MQ=255)

      ataataAGTCCATTGTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGtttt                                                                                   <  2:4480184/93‑1 (MQ=255)

        aataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGtttttt                                                                                 >  1:2334853/1‑93 (MQ=255)

         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCGTtttttt                                                                                 >  2:3438471/1‑93 (MQ=255)

         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCGTtttttt                                                                                 >  1:6011793/1‑93 (MQ=255)

          taaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttct                                                                               >  2:5284971/1‑93 (MQ=255)

          aaaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttct                                                                               <  1:4765888/92‑1 (MQ=255)

            aGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttcttc                                                                             <  2:3314687/93‑1 (MQ=255)

             gTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTtcttct                                                                            <  2:5688224/93‑1 (MQ=255)

             gTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTtcttct                                                                            >  1:2548120/1‑93 (MQ=255)

             gTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTtcttct                                                                            <  2:5152754/93‑1 (MQ=255)

              tCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTg                                                                           <  1:5560281/93‑1 (MQ=255)

              tCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTg                                                                           >  1:15485/1‑93 (MQ=255)

                cATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTc                                                                         >  2:1601462/1‑93 (MQ=255)

                cATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTc                                                                         >  2:815960/1‑93 (MQ=255)

                 aTTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCg                                                                        >  1:1174185/1‑93 (MQ=255)

                    ttttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCa                                                                     >  2:2068246/1‑93 (MQ=255)

                     tttAAACGTAGGGAGGCCGCGACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAt                                                                    <  1:1720654/93‑1 (MQ=255)

                     tttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAt                                                                    <  1:6140377/93‑1 (MQ=255)

                     tttAAACGGAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGCTTTTTCTTCTGTCGCCAt                                                                    >  1:3389889/1‑93 (MQ=255)

                      ttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAtc                                                                   <  1:168472/93‑1 (MQ=255)

                      ttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAtc                                                                   <  2:771846/93‑1 (MQ=255)

                        aaaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAtctt                                                                 >  2:469293/1‑92 (MQ=255)

                        aaaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAtctc                                                                 >  2:1888700/1‑93 (MQ=255)

                        aaaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAtctc                                                                 >  1:5194724/1‑93 (MQ=255)

                         aaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATctct                                                                <  2:2426945/93‑1 (MQ=255)

                         aaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATctct                                                                <  2:283400/93‑1 (MQ=255)

                          aCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCtt                                                               >  1:3640449/1‑93 (MQ=255)

                           cGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCttt                                                              <  1:4201183/93‑1 (MQ=255)

                            gTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTa                                                             >  2:529591/1‑93 (MQ=255)

                            gTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTa                                                             <  1:5076492/93‑1 (MQ=255)

                            gTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTa                                                             >  1:2576518/1‑93 (MQ=255)

                             tAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCGTTAt                                                            >  1:5465029/1‑93 (MQ=255)

                               gggAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAttt                                                          <  1:506785/93‑1 (MQ=255)

                               gggAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAttt                                                          >  1:5277310/1‑93 (MQ=255)

                               gggAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAttt                                                          >  2:5929658/1‑93 (MQ=255)

                               gggAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAttt                                                          >  2:1278248/1‑93 (MQ=255)

                               gggAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAttt                                                          >  1:3768418/1‑93 (MQ=255)

                                ggAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAtttt                                                         <  1:2173762/93‑1 (MQ=255)

                                   ggCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGc                                                      <  1:3287117/93‑1 (MQ=255)

                                     ccgccACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgt                                                    <  1:1935500/93‑1 (MQ=255)

                                       gccACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttg                                                  >  1:5797768/1‑93 (MQ=255)

                                          acccaccAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttggtt                                               <  1:3683886/93‑1 (MQ=255)

                                           cccaccAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttggttg                                              >  1:2121523/1‑93 (MQ=255)

                                              accaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAtt                                           <  1:3053486/93‑1 (MQ=255)

                                              accaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAtt                                           >  1:5476539/1‑93 (MQ=255)

                                               ccaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAttt                                          <  1:970033/93‑1 (MQ=255)

                                                caGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTc                                         >  1:4495741/1‑93 (MQ=255)

                                                caGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTc                                         <  2:5556246/93‑1 (MQ=255)

                                                 aGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTcc                                        >  1:1328309/1‑93 (MQ=255)

                                                 aGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTcc                                        >  2:1625690/1‑93 (MQ=255)

                                                  ggATGGAAATAGCGGCTATTGACCTTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCt                                       >  2:2308225/1‑93 (MQ=255)

                                                  ggATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCt                                       <  2:539545/93‑1 (MQ=255)

                                                     tGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCaa                                    >  2:5477671/1‑93 (MQ=255)

                                                       gAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCaata                                  <  2:1546687/93‑1 (MQ=255)

                                                       gAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCaata                                  >  2:3999897/1‑93 (MQ=255)

                                                        aaaTAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCaataa                                 <  1:2261525/93‑1 (MQ=255)

                                                         aaTAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCaataat                                >  1:2932329/1‑93 (MQ=255)

                                                          aTAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATc                               <  2:1402448/93‑1 (MQ=255)

                                                           tAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCa                              >  1:5698941/1‑93 (MQ=255)

                                                           tAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCGCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCa                              <  2:3759801/93‑1 (MQ=255)

                                                            aGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCaa                             <  2:3639337/93‑1 (MQ=255)

                                                            aGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCaa                             >  1:4269358/1‑93 (MQ=255)

                                                             gCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAAt                            >  2:4640067/1‑93 (MQ=255)

                                                               ggCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGc                          >  1:2724873/1‑93 (MQ=255)

                                                               ggCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGc                          >  1:2792592/1‑93 (MQ=255)

                                                                gCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCa                         >  2:3727249/1‑93 (MQ=255)

                                                                 cTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCaa                        >  1:5884743/1‑93 (MQ=255)

                                                                   aTTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATCCAAAc                      <  1:6101206/93‑1 (MQ=255)

                                                                     tGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCg                    >  2:4313360/1‑93 (MQ=255)

                                                                      gACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGt                   >  2:5594057/1‑93 (MQ=255)

                                                                       gcATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGtt                  <  1:2085421/92‑1 (MQ=255)

                                                                       aCATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGtt                  >  2:2774577/1‑93 (MQ=255)

                                                                        cATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACAGttt                 <  2:2934753/93‑1 (MQ=255)

                                                                          tttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGttttt               <  1:751377/93‑1 (MQ=255)

                                                                          tttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGttttt               >  2:2161803/1‑93 (MQ=255)

                                                                          tttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGttttt               >  2:306773/1‑93 (MQ=255)

                                                                          tttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGttttt               >  2:842889/1‑93 (MQ=255)

                                                                           ttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTc              >  1:5345446/1‑93 (MQ=255)

                                                                           ttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAACCAATGCAAACCGTTTTTc              <  2:2119721/93‑1 (MQ=255)

                                                                            tataACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCa             <  2:2577972/93‑1 (MQ=255)

                                                                             ataACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCAt            >  1:4333252/1‑93 (MQ=255)

                                                                              taACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCAtt           >  1:4579426/1‑93 (MQ=255)

                                                                              taACGTTACGGAAG‑CCGGTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCAtt           <  1:3003371/93‑1 (MQ=255)

                                                                                aCGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGa         <  1:1495315/93‑1 (MQ=255)

                                                                                aCGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGa         <  2:3842188/93‑1 (MQ=255)

                                                                                 cGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAg        <  2:3867481/93‑1 (MQ=255)

                                                                                  gTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAgt       >  2:90664/1‑93 (MQ=255)

                                                                                   ttACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAgtg      <  2:5277677/93‑1 (MQ=255)

                                                                                   ttACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAgtg      >  2:4258836/1‑93 (MQ=255)

                                                                                   ttACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAgtg      >  2:2895388/1‑93 (MQ=255)

                                                                                   ttACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTATTTCATTGAgtg      >  1:1940172/1‑93 (MQ=255)

                                                                                     aCGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAgtgtg    >  2:3410926/1‑93 (MQ=255)

                                                                                       ggAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTa  <  1:645919/93‑1 (MQ=255)

                                                                                       ggAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTa  >  2:2255253/1‑93 (MQ=255)

TTTTTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTA  >  NC_000913/4061867‑4062047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`