BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,542,643	Δ1 bp	intergenic (+46/‑22)	b4316 → / → fimD	Pil/FimD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,542,640	0	G	.	99.1%	992.5 / ‑0.3	214	intergenic (+43/‑25)	b4316/fimD	Pil/FimD
Reads supporting (aligned to +/- strand): ref base G (2/0); new base . (89/123); total (91/123)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 228 total aligned reads displayed.

TAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCATC  >  NC_000913/4542548‑4542728

tAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCg                                                                                          >  1:2790652/1‑93 (MQ=255)

  aaTGATTATGGCGCACTTACCCCCAAAATGACGGGCATAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCggg                                                                                        >  2:1029851/1‑93 (MQ=255)

   aTGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCgggt                                                                                       <  1:1778835/93‑2 (MQ=255)

     gATTATGGCGCACTTGCCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCgggtga                                                                                     <  2:2367528/93‑4 (MQ=255)

      aTTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCgggtgat                                                                                    <  2:138552/93‑5 (MQ=255)

      aTTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCgggtgat                                                                                    <  2:1660520/93‑5 (MQ=255)

         aTGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGAtttt                                                                                >  2:1105491/1‑93 (MQ=255)

           ggCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTaa                                                                              <  1:3250977/93‑1 (MQ=255)

            gcgcACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAg                                                                             <  1:5724198/93‑1 (MQ=255)

             cgcACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGc                                                                            <  2:223549/93‑1 (MQ=255)

              gcACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGcc                                                                           >  2:6048191/1‑93 (MQ=255)

              gcACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGcc                                                                           >  2:2129827/1‑93 (MQ=255)

               cACTTACCCCCAAAATGACGCGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCg                                                                          >  1:1854288/1‑93 (MQ=255)

                 cTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCgga                                                                        <  1:2201449/93‑1 (MQ=255)

                 cTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCgga                                                                        <  2:2892845/93‑1 (MQ=255)

                 cTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCgga                                                                        <  1:740962/93‑1 (MQ=255)

                 cTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCgga                                                                        <  1:5902488/93‑1 (MQ=255)

                   tACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCggagg                                                                      >  1:2592842/1‑93 (MQ=255)

                   tACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCggagg                                                                      <  2:391250/93‑1 (MQ=255)

                     cccccAAAATGACGGGCGTAATTGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaa                                                                    <  1:2066715/93‑1 (MQ=255)

                     cccccAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaa                                                                    <  1:215235/93‑1 (MQ=255)

                      ccccAAAATGACGGGCGTAATGGCATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaat                                                                   <  1:3488271/93‑1 (MQ=255)

                        ccAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaataa                                                                 <  1:4076788/93‑1 (MQ=255)

                         cAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaataat                                                                >  2:1878563/1‑93 (MQ=255)

                           aaaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGG‑GCTTTTAAGCCGGAGGAATAATGt                                                              >  1:1103218/1‑93 (MQ=255)

                           aaaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGt                                                              <  1:5795108/93‑1 (MQ=255)

                            aaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTc                                                             <  1:250768/93‑1 (MQ=255)

                             aTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCa                                                            <  2:4820179/93‑1 (MQ=255)

                             aTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCa                                                            <  1:3137991/93‑1 (MQ=255)

                                  gggCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCt                                                       >  2:1795845/1‑93 (MQ=255)

                                  gggCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCt                                                       <  1:3784629/93‑1 (MQ=255)

                                   ggCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTg                                                      >  2:5195760/1‑93 (MQ=255)

                                   ggCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTg                                                      <  1:5998277/93‑1 (MQ=255)

                                   ggCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTg                                                      >  1:4311943/1‑93 (MQ=255)

                                     cGTAATGCAATCACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAAGTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGaa                                                    <  2:2689391/93‑1 (MQ=255)

                                      gTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAAt                                                   >  1:3316280/1‑93 (MQ=255)

                                       tAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAAtt                                                  <  2:5244548/93‑1 (MQ=255)

                                         aTGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTa                                                >  1:436322/1‑93 (MQ=255)

                                          tGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTaa                                               >  1:4865729/1‑93 (MQ=255)

                                            gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGa                                             >  2:5533620/1‑93 (MQ=255)

                                            gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGa                                             <  1:2328574/93‑1 (MQ=255)

                                             aaTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGAc                                            >  1:5512051/1‑93 (MQ=255)

                                               tAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACtt                                          <  2:5693883/93‑1 (MQ=255)

                                                 aCGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTa                                        <  1:4971281/93‑1 (MQ=255)

                                                  cGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTAc                                       <  1:1425745/93‑1 (MQ=255)

                                                  cGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTAc                                       <  1:3916410/93‑1 (MQ=255)

                                                   gCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTAcc                                      <  1:3435745/93‑1 (MQ=255)

                                                    cAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATACTGTCATATCTGAATTTCAGACTTTACCa                                     >  2:823535/1‑93 (MQ=255)

                                                    cAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCa                                     <  2:2306900/93‑1 (MQ=255)

                                                    cAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCa                                     <  1:1190083/93‑1 (MQ=255)

                                                    cAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTACC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCa                                     >  2:3985323/1‑93 (MQ=255)

                                                     aGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAg                                    >  2:5429530/1‑93 (MQ=255)

                                                     aGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAg                                    >  1:2295495/1‑93 (MQ=255)

                                                      gggggAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGc                                   >  2:5894966/1‑93 (MQ=255)

                                                       ggggAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCg                                  >  1:3575244/1‑93 (MQ=255)

                                                       ggggAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCg                                  >  2:5948671/1‑93 (MQ=255)

                                                          gAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGaaa                               <  2:1279367/93‑1 (MQ=255)

                                                          gAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGaaa                               <  2:693546/93‑1 (MQ=255)

                                                           aaTTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAac                              <  1:2848541/93‑1 (MQ=255)

                                                           aaTTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAac                              >  1:805179/1‑93 (MQ=255)

                                                            aTTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAaca                             <  2:444188/93‑1 (MQ=255)

                                                            aTTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAaca                             <  2:630341/93‑1 (MQ=255)

                                                              ttttCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAacaca                           <  2:198656/93‑1 (MQ=255)

                                                                 tCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACaa                        <  1:3377025/93‑1 (MQ=255)

                                                                 tCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACaa                        <  1:3544106/93‑1 (MQ=255)

                                                                  cGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAAt                       <  1:1690382/93‑1 (MQ=255)

                                                                   gCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATg                      <  1:1376361/93‑1 (MQ=255)

                                                                   gCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATg                      >  2:5040193/1‑93 (MQ=255)

                                                                    ccTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGc                     >  1:5887280/1‑93 (MQ=255)

                                                                     cTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCt                    >  2:3347315/1‑93 (MQ=255)

                                                                      tGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCtt                   <  2:855835/93‑1 (MQ=255)

                                                                      tGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCtt                   >  2:5612255/1‑93 (MQ=255)

                                                                      tGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCtt                   >  2:1591936/1‑93 (MQ=255)

                                                                       gAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTg                  >  2:1260974/1‑93 (MQ=255)

                                                                        aaTAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGc                 <  2:903386/93‑1 (MQ=255)

                                                                        aaTAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGc                 >  2:3752555/1‑93 (MQ=255)

                                                                        aaTAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGc                 <  2:3376397/93‑1 (MQ=255)

                                                                        aaTAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGc                 <  1:2024656/93‑1 (MQ=255)

                                                                         aTAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCa                >  1:5648650/1‑93 (MQ=255)

                                                                         aTAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCa                >  1:3174648/1‑93 (MQ=255)

                                                                          tAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCat               <  1:2967100/93‑1 (MQ=255)

                                                                           aaaaaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCatt              >  2:5381878/1‑92 (MQ=255)

                                                                           aaaaaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCata              >  1:4197091/1‑93 (MQ=255)

                                                                           aaaaaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCata              >  1:4744974/1‑93 (MQ=255)

                                                                           aaaaaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCata              <  2:3161503/93‑1 (MQ=255)

                                                                           aaaaaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCata              <  2:4913511/93‑1 (MQ=255)

                                                                           aaaaaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCata              >  2:3414473/1‑93 (MQ=255)

                                                                            aaaaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCatat             >  2:5324011/1‑93 (MQ=255)

                                                                             aaaGAATTGACTGCC‑GGGTGATTTTAAGGCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATAtt            <  2:4739755/93‑1 (MQ=255)

                                                                              aaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTc           <  2:4098684/93‑1 (MQ=255)

                                                                               aGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCg          <  1:4926944/93‑1 (MQ=255)

                                                                                 aaTTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTa        >  1:3025879/1‑93 (MQ=255)

                                                                                  aTTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTaa       <  1:5402896/93‑1 (MQ=255)

                                                                                  aTTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTaa       >  2:4573598/1‑93 (MQ=255)

                                                                                   ttGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAg      >  1:517617/1‑93 (MQ=255)

                                                                                    tGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGc     <  1:4911246/93‑1 (MQ=255)

                                                                                     gACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCa    <  1:1005544/93‑1 (MQ=255)

                                                                                      aCTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCAt   <  2:715095/93‑1 (MQ=255)

                                                                                       cTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCATc  <  2:5858823/93‑1 (MQ=255)

                                                                                       cTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCATc  >  1:5223755/1‑93 (MQ=255)

TAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCATC  >  NC_000913/4542548‑4542728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`