Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,729,373 +C intergenic (‑195/+247) rrsG ← / ← clpB 16S ribosomal RNA/ClpB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,729,3711.C99.3% 445.6 / ‑3.8 136intergenic (‑193/+249)rrsG/clpB16S ribosomal RNA/ClpB
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (65/70);  total (65/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

AAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TT‑CCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTG  >  NC_000913/2729307‑2729456
                                                                  |                                                                                     
aaaTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGc                                                            <  2:4811909/93‑1 (MQ=0)
aaaTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGc                                                            <  1:2914058/93‑1 (MQ=0)
 aaTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCt                                                           <  1:395775/93‑1 (MQ=9)
 aaTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCt                                                           >  2:5909820/1‑93 (MQ=0)
 aaTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCt                                                           >  1:2000124/1‑93 (MQ=0)
  aTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtt                                                          <  1:2267364/93‑1 (MQ=12)
  aTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtt                                                          >  1:5419969/1‑93 (MQ=9)
  aTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtt                                                          >  2:5545588/1‑93 (MQ=9)
  aTTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtt                                                          >  2:2631348/1‑93 (MQ=9)
   ttGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCttt                                                         <  1:4103476/93‑1 (MQ=16)
   ttGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCttt                                                         <  1:4717209/93‑1 (MQ=16)
    tGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                                                        <  1:2243558/93‑1 (MQ=16)
    tGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                                                        >  2:5055473/1‑93 (MQ=16)
    tGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                                                        >  2:3989668/1‑93 (MQ=16)
    tGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                                                        <  1:3106243/93‑1 (MQ=255)
     gTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                                                       >  2:5503193/1‑93 (MQ=255)
      ttgaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtct                                                      >  2:5358355/4‑93 (MQ=255)
       tAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                                                     <  2:5584814/93‑1 (MQ=255)
        aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                    <  2:444058/93‑1 (MQ=255)
        aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                    >  1:1067526/1‑93 (MQ=255)
        aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                    >  1:5378156/1‑93 (MQ=255)
        aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                    >  2:4363418/1‑93 (MQ=255)
        aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                    <  1:979543/93‑1 (MQ=255)
        aaaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                                                    <  1:1962556/93‑1 (MQ=255)
         aaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTg                                                   >  1:3093256/1‑93 (MQ=255)
         aaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTg                                                   <  1:5037772/93‑1 (MQ=255)
         aaGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTg                                                   >  1:4852763/1‑93 (MQ=255)
          agagCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGt                                                  >  2:3912349/1‑93 (MQ=255)
           gagCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                                                 <  1:384391/93‑1 (MQ=255)
           gagCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                                                 <  1:2428490/93‑1 (MQ=255)
           gagCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGAGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGtt                                                 >  1:1859043/1‑93 (MQ=255)
             gCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                                               <  1:293513/93‑1 (MQ=255)
             gCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                                               <  2:3110469/93‑1 (MQ=255)
             gCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                                               <  1:4553381/93‑1 (MQ=255)
             gCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                                               <  1:4442264/93‑1 (MQ=255)
               aGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga                                             <  2:3460966/93‑1 (MQ=255)
               aGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga                                             <  2:4833822/93‑1 (MQ=255)
                gTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt                                            <  2:4724145/93‑1 (MQ=255)
                gTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGGGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt                                            >  2:2127763/1‑93 (MQ=255)
                 tGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAtt                                           <  2:2673282/93‑1 (MQ=255)
                  gCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtc                                          >  2:2816457/1‑93 (MQ=255)
                   ccGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtct                                         >  2:2671835/1‑93 (MQ=255)
                    cgcttcgcttTTTCTCAGCGGCGCGGGGTGTGGATAATACGCCGTC‑CCGCTCCAGACTCAAGCATTTCTTTGCTTTTCTCTGTTTAGATtctc                                        >  2:62343/1‑93 (MQ=17)
                    cgcttcgcttTTTCTCAGCGGCGCGGGGGGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc                                        >  2:62344/1‑93 (MQ=255)
                     gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                       >  1:2434884/1‑93 (MQ=255)
                     gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                       <  1:5911628/93‑1 (MQ=255)
                     gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                       >  2:5093155/1‑93 (MQ=255)
                     gcttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa                                       >  2:4883417/1‑93 (MQ=255)
                      cttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg                                      <  1:3985631/93‑1 (MQ=255)
                      cttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg                                      <  1:5065187/93‑1 (MQ=255)
                      cttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg                                      >  2:2876579/1‑93 (MQ=255)
                       ttcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg                                     >  1:4717541/1‑93 (MQ=255)
                        tcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga                                    <  1:1304978/93‑1 (MQ=255)
                        tcgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga                                    <  1:4003997/93‑1 (MQ=255)
                        tagcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga                                    <  2:2263663/91‑1 (MQ=255)
                         cgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag                                   >  1:634650/1‑93 (MQ=255)
                         cgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag                                   <  2:3973459/93‑1 (MQ=255)
                         cgcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag                                   <  2:3973458/93‑1 (MQ=255)
                          gcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga                                  <  1:4250557/93‑1 (MQ=255)
                          gcttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga                                  <  2:2304108/93‑1 (MQ=255)
                           cttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgagga                                 <  1:4311475/93‑3 (MQ=255)
                           cttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGaa                                 >  2:5389294/1‑93 (MQ=255)
                           cttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGaa                                 >  2:5507528/1‑93 (MQ=255)
                            tttttCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc                                <  1:2755921/93‑1 (MQ=255)
                             ttttCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcc                               >  2:239759/1‑93 (MQ=255)
                             ttttCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCACTGTTGAGATTCTCAGGAGAAcc                               >  1:4492902/1‑93 (MQ=255)
                             ttttCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAAAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcc                               >  2:2086388/1‑93 (MQ=255)
                               ttCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcccc                             <  1:4760943/93‑1 (MQ=255)
                                 ctcAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCccgc                           <  2:3888205/93‑1 (MQ=255)
                                  tcAGCGGCGCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCccgcc                          <  2:904540/93‑1 (MQ=255)
                                     gcggcgCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAc                       >  1:3586375/1‑93 (MQ=255)
                                     gcggcgCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAc                       >  1:3878037/1‑93 (MQ=255)
                                     gcggcgCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAc                       >  2:1519428/1‑93 (MQ=255)
                                     gcggcgCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAc                       >  1:5121989/1‑93 (MQ=255)
                                     gcggcgCGGGGGGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAc                       >  1:4054575/1‑93 (MQ=255)
                                      cggcgCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAcc                      >  1:1268075/1‑93 (MQ=255)
                                      cggcgCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAcc                      <  1:1736075/93‑1 (MQ=255)
                                       ggcgCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAccc                     <  1:5026355/93‑1 (MQ=255)
                                       ggcgCGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAccc                     <  1:2457701/93‑1 (MQ=255)
                                        gcgcGGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcg                    <  1:2222896/93‑1 (MQ=255)
                                          gcgGGGTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggc                  >  1:4497676/1‑93 (MQ=255)
                                            ggggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcgg                >  2:30747/1‑93 (MQ=255)
                                            ggggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcgg                >  1:2574926/1‑93 (MQ=255)
                                            ggggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcgg                >  2:1623465/1‑93 (MQ=255)
                                             gggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCggcggc               >  2:4268494/1‑93 (MQ=255)
                                             gggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCggcggc               >  1:4153515/1‑93 (MQ=255)
                                              ggTGTGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGgcggcg              >  1:873894/1‑93 (MQ=255)
                                               gtgtGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgt             <  1:3490610/93‑1 (MQ=255)
                                               gtgtGCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgt             >  2:646417/1‑93 (MQ=255)
                                                tgtgCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCgtg            >  2:880533/1‑93 (MQ=255)
                                                  tgCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGtt          >  2:5036962/1‑93 (MQ=255)
                                                  tgCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGtt          <  1:1909312/93‑1 (MQ=255)
                                                  tgCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGtt          >  2:332298/1‑93 (MQ=255)
                                                   gCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGTTTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGttt         <  2:498535/93‑1 (MQ=255)
                                                   gCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGttt         <  2:153149/93‑1 (MQ=255)
                                                   gCATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGttt         <  1:2897750/93‑1 (MQ=255)
                                                    cATAATACGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTg        <  1:3566097/93‑1 (MQ=255)
                                                      taataCGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGcc      >  2:1130875/1‑93 (MQ=255)
                                                      taataCGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGcc      <  2:5223414/93‑1 (MQ=255)
                                                      taataCGCC‑TTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGcc      >  1:4454349/1‑93 (MQ=255)
                                                                  |                                                                                     
AAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCC‑TT‑CCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTG  >  NC_000913/2729307‑2729456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑