BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,838,044	+G	coding (498/1458 nt)	ascF →	PTS system enzyme IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,043	1	.	G	99.6%	810.3 / ‑4.3	226	coding (497/1458 nt)	ascF	PTS system enzyme IIBC
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (112/113); total (112/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

GATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGC‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTG  >  NC_000913/2837951‑2838131

gaTGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc                                                                                           >  1:4116332/1‑93 (MQ=255)

gaTGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc                                                                                           >  2:2265344/1‑93 (MQ=255)

gaTGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc                                                                                           <  2:4857616/93‑1 (MQ=255)

  tGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑gg                                                                                        <  2:804122/93‑2 (MQ=255)

   gAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑ggc                                                                                       <  2:3603902/93‑3 (MQ=255)

     gcggcgTGCTGACAAAAGGATCGCCGACCTTAACCATTCGGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑ggcat                                                                                     <  1:3150183/93‑5 (MQ=255)

         cgTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGc                                                                                  <  1:3318388/93‑1 (MQ=255)

          gTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGcc                                                                                 >  1:2052491/1‑93 (MQ=255)

          gTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGcc                                                                                 <  2:3437947/93‑1 (MQ=255)

           tGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcg                                                                                >  2:2872636/1‑93 (MQ=255)

           tGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcg                                                                                >  1:5505358/1‑93 (MQ=255)

           tGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcg                                                                                >  2:1106714/1‑93 (MQ=255)

           tGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcg                                                                                >  2:1042997/1‑93 (MQ=255)

             cTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgcc                                                                              <  1:4222238/93‑2 (MQ=255)

              tGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgcca                                                                             >  2:1235812/1‑91 (MQ=255)

              tGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgcca                                                                             <  1:389500/93‑3 (MQ=255)

               gACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgccat                                                                            >  1:1696037/1‑90 (MQ=255)

               gACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgccat                                                                            <  2:4196988/93‑4 (MQ=255)

               gACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgcaat                                                                            >  2:6054399/1‑90 (MQ=255)

                aCAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgccatc                                                                           <  2:3705816/93‑5 (MQ=255)

                 cAAAAGGATCGCCGCCCTTAACCATTATTAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCCCTGATGGTCGCGGCATCTGCCGC‑CATCa                                                                         <  1:660829/93‑1 (MQ=255)

                  aaaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCaa                                                                        <  1:1045407/93‑1 (MQ=255)

                   aaaGGATGGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCaaa                                                                       <  2:2616380/93‑1 (MQ=255)

                   aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCaaa                                                                       <  1:5571990/93‑1 (MQ=255)

                    aaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAt                                                                      <  2:639573/93‑1 (MQ=255)

                     aGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAtt                                                                     >  2:3058929/1‑93 (MQ=255)

                      ggATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAttt                                                                    <  2:2949368/93‑1 (MQ=255)

                        aTCGCCGGCCTTAACCATTCTGAATGTGATTGGTGACTGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaa                                                                  <  2:3328295/93‑1 (MQ=255)

                        aTCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaa                                                                  <  2:1683999/93‑1 (MQ=255)

                           gCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAAc                                                               <  2:3370458/93‑1 (MQ=255)

                             cGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCa                                                             >  1:5186403/1‑93 (MQ=255)

                              gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                            <  1:1155390/93‑1 (MQ=255)

                               aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                                           <  1:1208212/93‑1 (MQ=255)

                               aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                                           <  1:2211618/93‑1 (MQ=255)

                               aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                                           <  2:727244/93‑1 (MQ=255)

                               aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                                           >  1:5922243/1‑93 (MQ=255)

                                ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAata                                                          >  2:2342652/1‑93 (MQ=255)

                                ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAAACAata                                                          >  1:2577424/1‑93 (MQ=255)

                                 cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                         >  1:1321681/1‑93 (MQ=255)

                                  ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                        >  1:896655/1‑93 (MQ=255)

                                  ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                        <  2:1878550/93‑1 (MQ=255)

                                  ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                        >  1:4578633/1‑93 (MQ=255)

                                   tAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAATCAATATGt                                                       >  2:892425/1‑93 (MQ=255)

                                    aaCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTc                                                      <  2:6007069/93‑1 (MQ=255)

                                    aaCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTc                                                      <  1:288923/93‑1 (MQ=255)

                                    aaCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTc                                                      >  1:3403666/1‑93 (MQ=255)

                                      ccATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGc                                                    >  2:2422553/1‑93 (MQ=255)

                                        aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                  <  2:4081254/93‑1 (MQ=255)

                                        aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                  <  1:2644688/93‑1 (MQ=255)

                                         ttCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTgg                                                 >  2:5330142/1‑93 (MQ=255)

                                          tCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGc                                                <  1:2145436/93‑1 (MQ=255)

                                          tCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGc                                                <  2:3639928/93‑1 (MQ=255)

                                          tCTGAATGTGATTGGTGACGGTGCTTTCTTCTGCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGc                                                <  1:2450090/93‑1 (MQ=255)

                                            tGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGa                                              >  1:1037356/1‑93 (MQ=255)

                                            tGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGa                                              >  2:2464242/1‑93 (MQ=255)

                                            tGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGa                                              <  2:1210905/93‑1 (MQ=255)

                                            tGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTCGCGa                                              >  2:1272599/1‑93 (MQ=255)

                                                tgtgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGc                                          <  2:402966/93‑1 (MQ=255)

                                                 gtgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGcc                                         >  1:779816/1‑93 (MQ=255)

                                                  tgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCa                                        >  1:4847788/1‑93 (MQ=255)

                                                  tgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCa                                        <  2:4983964/93‑1 (MQ=255)

                                                   gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                       <  1:1077787/93‑1 (MQ=255)

                                                   gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                       <  1:1705243/93‑1 (MQ=255)

                                                    aTTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGTCGATTGCCATc                                      >  1:3259327/1‑93 (MQ=255)

                                                     ttGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcg                                     >  2:5896242/1‑93 (MQ=255)

                                                        gTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCgg                                  <  2:4505174/93‑1 (MQ=255)

                                                          gACGTTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgt                                <  2:4446384/93‑1 (MQ=255)

                                                             ggTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGtgtg                             >  1:87712/1‑93 (MQ=255)

                                                              gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGTCATCGCGGGTGTGc                            >  2:4973586/1‑93 (MQ=255)

                                                              gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                            >  2:4558829/1‑93 (MQ=255)

                                                              gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCAACTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                            >  1:6061058/1‑93 (MQ=255)

                                                                gCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTg                          >  1:5675965/1‑93 (MQ=255)

                                                                 cTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCCTCGCGGGTGTGCTgg                         >  2:3875313/1‑93 (MQ=255)

                                                                  tttCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGt                        >  1:4071262/1‑93 (MQ=255)

                                                                   ttcttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTa                       >  2:2739515/1‑93 (MQ=255)

                                                                       tcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATc                   >  2:4712470/1‑93 (MQ=255)

                                                                       tcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATc                   >  1:4685387/1‑93 (MQ=255)

                                                                       tcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATc                   >  1:1472712/1‑93 (MQ=255)

                                                                        cttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATcc                  >  2:2517782/1‑93 (MQ=255)

                                                                          tcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGa                <  1:3645983/93‑1 (MQ=255)

                                                                           ccTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAg               >  2:426836/1‑93 (MQ=255)

                                                                           ccTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAg               <  2:2571261/93‑1 (MQ=255)

                                                                            cTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGGGCTGGTACATCCGAGt              >  2:1438809/1‑93 (MQ=255)

                                                                             tGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtt             >  2:5494412/1‑93 (MQ=255)

                                                                             tGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtt             <  2:5233678/93‑1 (MQ=255)

                                                                             tGCCGCTGAAGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtt             >  1:4185611/1‑93 (MQ=255)

                                                                              gCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGttt            >  1:1829194/1‑93 (MQ=255)

                                                                               ccGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtttt           >  1:1531422/1‑93 (MQ=255)

                                                                                cGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTa          <  1:5861805/93‑1 (MQ=255)

                                                                                 gCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAt         >  2:4852472/1‑93 (MQ=255)

                                                                                 gCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGCGTGCTGGTACATCCGAGTTTTAt         >  2:5768378/1‑93 (MQ=255)

                                                                                  cTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAtt        >  1:2875202/1‑93 (MQ=255)

                                                                                  cTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAtt        <  2:1476246/93‑1 (MQ=255)

                                                                                  cTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAtt        >  2:2799128/1‑93 (MQ=255)

                                                                                  cTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAtt        <  1:312282/93‑1 (MQ=255)

                                                                                   tGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg       >  2:1777514/1‑93 (MQ=255)

                                                                                    gATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGa      <  1:5046500/93‑1 (MQ=255)

                                                                                    gATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGa      >  2:2331684/1‑93 (MQ=255)

                                                                                     aTGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGaa     <  2:3234457/93‑1 (MQ=255)

                                                                                        gTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTg  >  2:1585587/1‑93 (MQ=255)

GATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGC‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTG  >  NC_000913/2837951‑2838131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`