BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,357	+G	coding (163/1314 nt)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,356	1	.	G	99.0%	680.3 / ‑1.1	191	coding (162/1314 nt)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (111/78); total (112/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 217 total aligned reads displayed.

GGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑GGACG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544264‑3544437

 gCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑g                                                                                      >  1:5433883/1‑93 (MQ=255)

 gCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑g                                                                                      >  2:5983513/1‑93 (MQ=255)

 gCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑g                                                                                      <  2:4790987/93‑1 (MQ=255)

   ttCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggc                                                                                    <  2:3131343/93‑3 (MQ=255)

   ttCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggc                                                                                    >  2:2182930/1‑91 (MQ=255)

   ttCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggc                                                                                    <  1:4186146/93‑3 (MQ=255)

    tCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggcg                                                                                   >  2:4683144/1‑90 (MQ=255)

                   tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGcc                                                                   <  1:4614757/93‑1 (MQ=255)

                    cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCt                                                                  <  2:25446/93‑1 (MQ=255)

                     gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCtt                                                                 >  1:2408873/1‑93 (MQ=255)

                      tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTg                                                                <  1:1891701/93‑1 (MQ=255)

                      tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTg                                                                >  1:1731385/1‑93 (MQ=255)

                        gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGcc                                                              >  1:1540008/1‑93 (MQ=255)

                         cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGccc                                                             <  2:3556180/93‑1 (MQ=255)

                          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCt                                                            <  2:4975420/93‑1 (MQ=255)

                          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCt                                                            >  1:5999959/1‑93 (MQ=255)

                           cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTg                                                           >  2:4713545/1‑93 (MQ=255)

                            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGa                                                          <  2:5934013/93‑1 (MQ=255)

                            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGa                                                          <  1:3743390/93‑1 (MQ=255)

                            ttGCTGTTGGATTAATGCAAGCAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGa                                                          <  2:3493194/93‑1 (MQ=255)

                            ctGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGa                                                          <  2:3672603/92‑1 (MQ=255)

                              gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATc                                                        >  2:1670609/1‑93 (MQ=255)

                              gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATc                                                        >  2:1515150/1‑93 (MQ=255)

                              gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATc                                                        >  2:438604/1‑93 (MQ=255)

                               cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGCTCa                                                       <  1:3069669/93‑1 (MQ=255)

                               cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCa                                                       >  2:2107246/1‑93 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATg                                                     <  2:1221220/93‑1 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:4990789/1‑93 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:5722343/1‑93 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:5156412/1‑93 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:3132548/1‑93 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:1515196/1‑93 (MQ=255)

                                  ttGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATgg                                                    >  2:5935203/1‑93 (MQ=255)

                                    ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGt                                                  >  2:364512/1‑93 (MQ=255)

                                    ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGt                                                  >  2:632017/1‑93 (MQ=255)

                                     gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGtt                                                 >  2:828326/1‑93 (MQ=255)

                                      aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGttt                                                <  2:4729732/93‑1 (MQ=255)

                                       ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               >  2:727937/1‑93 (MQ=255)

                                        tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTg                                              >  1:1986682/1‑93 (MQ=255)

                                        tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTg                                              >  1:337998/1‑93 (MQ=255)

                                        tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTg                                              <  2:4440004/93‑1 (MQ=255)

                                         aaTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTgg                                             <  2:5762854/93‑1 (MQ=255)

                                           tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcg                                           >  2:4514608/1‑93 (MQ=255)

                                             cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  2:3930323/1‑93 (MQ=255)

                                             cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         <  2:3778479/93‑1 (MQ=255)

                                              aaGGAGTGCCGCGGGATAAAGTCATTGGCTCCCACAAATCCGGTGTC‑GGCC‑GGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  2:381434/93‑1 (MQ=255)

                                              aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  2:4573521/93‑1 (MQ=255)

                                              aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        >  2:207511/1‑93 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGCCGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  1:5077361/1‑93 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  1:955431/1‑93 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGCGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  1:2605782/1‑93 (MQ=255)

                                                ggAATGCCGCTGGATAAAGTTATTGGCTCCATCCAAGCCGGTGGCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAtc                                      >  1:3721968/1‑92 (MQ=255)

                                                ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      >  1:5561824/1‑93 (MQ=255)

                                                ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATg                                      >  2:5823908/1‑93 (MQ=255)

                                                 gAATGCCTCTGGATAAAGTTATTGGCTCCAACAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  1:3372024/93‑1 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     >  1:5873257/1‑93 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  2:1614673/93‑1 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     >  2:5301429/1‑93 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  2:3095506/93‑1 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGc                                     >  1:2443793/1‑93 (MQ=255)

                                                   aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTTGCGCAGTGCTg                                   >  2:3441761/1‑93 (MQ=255)

                                                   aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   <  2:1715232/93‑1 (MQ=255)

                                                     gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTggg                                 >  2:5445313/1‑93 (MQ=255)

                                                       cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                               >  1:1361194/1‑93 (MQ=255)

                                                       cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                               >  1:476361/1‑93 (MQ=255)

                                                        gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  2:793543/1‑93 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           >  1:1711943/1‑93 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           <  2:5902033/93‑1 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           <  1:4882184/93‑1 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           >  1:755656/1‑93 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           >  2:3432214/1‑93 (MQ=255)

                                                            gATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                          >  2:1379295/1‑93 (MQ=255)

                                                              tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGcg                        >  1:5340229/1‑92 (MQ=255)

                                                              tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        >  2:4047774/1‑93 (MQ=255)

                                                                 aGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGc                     >  2:4463641/1‑93 (MQ=255)

                                                                 aGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGc                     <  2:5530199/93‑1 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCTGGTGTCGG‑‑CGGGACGCTTGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAggc                 <  1:1559252/93‑3 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCACAATGCTGGCAGAc                 >  2:2613324/1‑93 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:5797284/93‑1 (MQ=255)

                                                                     aTTGGCGCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:188457/93‑1 (MQ=255)

                                                                      ttGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                <  1:1263418/93‑1 (MQ=255)

                                                                      ttGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                <  1:5171259/93‑1 (MQ=255)

                                                                       tGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               >  1:1091499/1‑93 (MQ=255)

                                                                        tgCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  1:2703960/92‑1 (MQ=255)

                                                                         gCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGACAGACTGCg             <  1:4183330/93‑1 (MQ=255)

                                                                          cTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg            >  2:293364/1‑93 (MQ=255)

                                                                          cTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg            <  1:3783475/93‑1 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  1:3434604/93‑1 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  2:1324790/93‑1 (MQ=255)

                                                                            ccATCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg          >  2:2287435/1‑93 (MQ=255)

                                                                              aTCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTggg        >  2:5566249/1‑92 (MQ=255)

                                                                              aTCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc        <  1:2916810/93‑1 (MQ=255)

                                                                               tCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  2:3534433/1‑93 (MQ=255)

                                                                               tCAAAGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  1:3411640/1‑93 (MQ=255)

                                                                                  aaGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  2:5228766/1‑93 (MQ=255)

                                                                                  aaGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  1:4393714/1‑93 (MQ=255)

                                                                                  aaGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    <  1:4428883/93‑1 (MQ=255)

                                                                                   aGCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   <  1:1157952/93‑1 (MQ=255)

                                                                                    gCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  2:5432354/93‑1 (MQ=255)

                                                                                    gCCGGTGTCGG‑‑CGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  1:5379596/93‑1 (MQ=255)

GGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑GGACG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544264‑3544437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`