BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,360	+G	coding (166/1314 nt)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,358	1	.	G	99.0%	673.0 / ‑1.2	190	coding (164/1314 nt)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (110/78); total (111/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 201 total aligned reads displayed.

TCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑GG‑AC‑G‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544283‑3544437

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGcc                                                                   <  1:4614757/93‑1 (MQ=255)

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGcc                                                                   <  2:4810560/93‑1 (MQ=255)

 cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCt                                                                  <  2:2584263/93‑1 (MQ=255)

  gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCtt                                                                 >  1:2408873/1‑93 (MQ=255)

     gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGcc                                                              >  1:1540008/1‑93 (MQ=255)

      cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGccc                                                             <  1:3367032/93‑1 (MQ=255)

       gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCt                                                            >  1:5885933/1‑93 (MQ=255)

        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCTGTAGCCTTGCCCTg                                                           >  2:5626078/1‑93 (MQ=255)

        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTg                                                           >  2:4713545/1‑93 (MQ=255)

        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTg                                                           >  1:2675158/1‑93 (MQ=255)

         ttGCTGTTGGATTAATGCAAGCAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGa                                                          <  2:3493194/93‑1 (MQ=255)

           gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATc                                                        >  2:438604/1‑93 (MQ=255)

            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGCTCa                                                       <  1:3069669/93‑1 (MQ=255)

            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCa                                                       >  2:2107246/1‑93 (MQ=255)

            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCa                                                       <  2:5819900/93‑1 (MQ=255)

             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCAt                                                      <  2:1929768/93‑1 (MQ=255)

             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCAt                                                      >  2:1679070/1‑93 (MQ=255)

              gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:4990789/1‑93 (MQ=255)

              gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:5156412/1‑93 (MQ=255)

              gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:1515196/1‑93 (MQ=255)

              gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATg                                                     <  2:1221220/93‑1 (MQ=255)

              gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:3132548/1‑93 (MQ=255)

                 ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTTCCCTGATCATGGGt                                                  >  2:364513/1‑93 (MQ=255)

                 ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGt                                                  >  2:632017/1‑93 (MQ=255)

                  gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGtt                                                 >  2:828326/1‑93 (MQ=255)

                   aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGttt                                                <  2:4729732/93‑1 (MQ=255)

                    ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               >  2:727937/1‑93 (MQ=255)

                     tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTg                                              >  1:1986682/1‑93 (MQ=255)

                     tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTg                                              >  1:5269967/1‑93 (MQ=255)

                     tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTg                                              <  2:4440004/93‑1 (MQ=255)

                        tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcg                                           >  1:1763363/1‑93 (MQ=255)

                        tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcg                                           >  2:3763007/1‑93 (MQ=255)

                        tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcg                                           >  2:3785519/1‑93 (MQ=255)

                        tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcg                                           >  2:4514608/1‑93 (MQ=255)

                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  2:3930323/1‑93 (MQ=255)

                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  2:1826604/1‑93 (MQ=255)

                           caGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  2:381634/92‑1 (MQ=255)

                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        >  1:5701269/1‑93 (MQ=255)

                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        >  2:207511/1‑93 (MQ=255)

                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        >  2:3578772/1‑93 (MQ=255)

                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  2:1550195/93‑1 (MQ=255)

                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  2:1486843/93‑1 (MQ=255)

                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑CC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  1:5077361/1‑93 (MQ=255)

                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  2:5934756/93‑1 (MQ=255)

                              gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  2:5790052/93‑1 (MQ=255)

                              gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  2:3095506/93‑1 (MQ=255)

                              gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  1:3372025/93‑1 (MQ=255)

                              gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     >  2:5301429/1‑93 (MQ=255)

                               aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    <  1:3775719/93‑1 (MQ=255)

                                aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTTGCGCAGTGCTg                                   >  2:3441761/1‑93 (MQ=255)

                                 tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  <  1:1603394/93‑1 (MQ=255)

                                 tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  >  2:4580398/1‑93 (MQ=255)

                                  gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTggg                                 >  2:3198178/1‑93 (MQ=255)

                                  gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTggg                                 >  2:5445313/1‑93 (MQ=255)

                                   ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCCTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                >  1:2451517/1‑93 (MQ=255)

                                    cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                               >  1:1361194/1‑93 (MQ=255)

                                     gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  2:793543/1‑93 (MQ=255)

                                       tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            >  2:35272/1‑93 (MQ=255)

                                        ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           >  2:4529740/1‑93 (MQ=255)

                                        ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           <  1:4882184/93‑1 (MQ=255)

                                         gATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                          >  2:1379295/1‑93 (MQ=255)

                                         gATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                          <  1:3397494/93‑1 (MQ=255)

                                           tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        >  2:2625069/1‑93 (MQ=255)

                                           tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        >  2:2203247/1‑93 (MQ=255)

                                           tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        >  2:4047774/1‑93 (MQ=255)

                                           tAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑GGAG‑GG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        <  2:5929132/93‑1 (MQ=255)

                                            aaaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       >  1:4666028/1‑93 (MQ=255)

                                            aaaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       <  1:3424791/93‑1 (MQ=255)

                                              aGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGc                     <  2:5530199/93‑1 (MQ=255)

                                              aGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGc                     <  2:5938132/93‑1 (MQ=255)

                                              aGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGc                     >  2:4463641/1‑93 (MQ=255)

                                               gTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                    >  1:5519777/1‑93 (MQ=255)

                                                 tATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGa                  <  1:2425223/93‑1 (MQ=255)

                                                  aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 >  1:1712804/1‑93 (MQ=255)

                                                  aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 >  1:3737535/1‑93 (MQ=255)

                                                  aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  1:1559251/93‑1 (MQ=255)

                                                   ttGGCTCTATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  1:3717411/1‑93 (MQ=255)

                                                   ttGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                <  1:5171259/93‑1 (MQ=255)

                                                   ttGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                <  1:1263418/93‑1 (MQ=255)

                                                   ttGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  1:892050/1‑93 (MQ=255)

                                                    tGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               <  2:1648373/93‑1 (MQ=255)

                                                    tGGCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               >  1:1091499/1‑93 (MQ=255)

                                                     tgCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  2:5166033/92‑1 (MQ=255)

                                                     ggCTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  2:3555579/93‑1 (MQ=255)

                                                      gcaccATCAAAGCCGGTGTCGG‑‑C‑GGCACAC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGTCGCAATGCTGGGTAAAATGCTGCCAGACTTCg             <  1:4183331/90‑1 (MQ=255)

                                                       cTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg            <  1:3783475/93‑1 (MQ=255)

                                                       cTCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg            >  2:293364/1‑93 (MQ=255)

                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCggg           >  1:744563/1‑92 (MQ=255)

                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  2:1324790/93‑1 (MQ=255)

                                                         ccATCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg          >  2:5827274/1‑93 (MQ=255)

                                                           aTCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTggg        >  2:5566249/1‑92 (MQ=255)

                                                            tCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  1:2748819/1‑93 (MQ=255)

                                                            tCAAAGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGACCATGGGTTTTGGCGCAATGCTGGGCAAAATGTTGGAAAATTGCGgggggc       >  1:5726781/1‑91 (MQ=255)

                                                               aaGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    <  1:4428883/93‑1 (MQ=255)

                                                               aaGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  1:4393714/1‑93 (MQ=255)

                                                               aaGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  2:5228766/1‑93 (MQ=255)

                                                                aGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   <  2:2307164/93‑1 (MQ=255)

                                                                aGCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   <  1:1157952/93‑1 (MQ=255)

                                                                 gCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  1:5379596/93‑1 (MQ=255)

                                                                 gCCGGTGTCGG‑‑CGGG‑AC‑GCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  2:5432354/93‑1 (MQ=255)

TCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑GG‑AC‑G‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544283‑3544437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`