BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,311,496	+C	coding (301/408 nt)	phnQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,311,495	1	.	C	99.2%	918.3 / ‑1.4	256	coding (302/408 nt)	phnQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (142/112); total (142/114)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 275 total aligned reads displayed.

AGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑C‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCG  >  NC_000913/4311404‑4311582

 gTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑cc                                                                                        <  1:5009175/93‑2 (MQ=255)

 gTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑cc                                                                                        <  2:2034849/93‑2 (MQ=255)

 gTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑cc                                                                                        <  1:2354784/93‑2 (MQ=255)

    aTGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑ccgtt                                                                                     <  2:4085731/93‑5 (MQ=255)

      gATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTcc                                                                                   <  1:2478027/93‑1 (MQ=255)

      gATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTcc                                                                                   <  2:4536707/93‑1 (MQ=255)

       aTTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCg                                                                                  <  1:3141175/93‑1 (MQ=255)

       aTTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCg                                                                                  >  2:5314786/1‑93 (MQ=255)

        ttAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCgg                                                                                 >  1:2941890/1‑93 (MQ=255)

         tAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGc                                                                                <  1:5294207/93‑1 (MQ=255)

           aTGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGccc                                                                              <  1:5365600/93‑1 (MQ=255)

            tgtgGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCa                                                                             <  1:2409295/93‑1 (MQ=255)

             gtgGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAg                                                                            <  1:2770597/93‑1 (MQ=255)

              tgGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAgg                                                                           <  1:683991/93‑1 (MQ=255)

               ggATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGc                                                                          <  1:5865200/93‑1 (MQ=255)

                gATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGcc                                                                         >  2:2389083/1‑93 (MQ=255)

                  tAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGa                                                                       <  2:4977563/93‑1 (MQ=255)

                  tAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGa                                                                       <  2:933826/93‑1 (MQ=255)

                  tAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGa                                                                       >  2:5793394/1‑93 (MQ=255)

                  tAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGa                                                                       >  1:1322563/1‑93 (MQ=255)

                  tAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGa                                                                       <  1:1522905/93‑1 (MQ=255)

                   agagTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGat                                                                      <  1:5633099/93‑1 (MQ=255)

                      gTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGAtatt                                                                   <  2:2779738/93‑1 (MQ=255)

                       tttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGAtatta                                                                  <  2:2556172/93‑1 (MQ=255)

                       tttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGAtatta                                                                  >  2:1038655/1‑93 (MQ=255)

                       tgtCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGAtatta                                                                  <  1:1382601/91‑1 (MQ=255)

                         tCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATc                                                                <  2:3391884/93‑1 (MQ=255)

                         tCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATc                                                                >  2:3325946/1‑93 (MQ=255)

                         tCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATc                                                                <  1:929104/93‑1 (MQ=255)

                         tCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATc                                                                >  2:565411/1‑93 (MQ=255)

                           tttttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATcga                                                              >  2:4052404/1‑93 (MQ=255)

                           tttttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATcga                                                              >  2:4352020/1‑93 (MQ=255)

                            ttttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATagac                                                             >  2:3680953/1‑93 (MQ=255)

                             tttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATcgacg                                                            <  1:1036554/93‑1 (MQ=255)

                                gAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGaca                                                         >  1:3595864/1‑93 (MQ=255)

                                  ggTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACAcc                                                       >  1:4152284/1‑93 (MQ=255)

                                    ttGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCAt                                                     <  1:4817019/93‑1 (MQ=255)

                                     tGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCAtt                                                    <  2:1620748/93‑1 (MQ=255)

                                     tGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCAtt                                                    >  1:2770335/1‑93 (MQ=255)

                                      ggCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTg                                                   <  2:263986/93‑1 (MQ=255)

                                         tAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCa                                                >  1:1451177/1‑93 (MQ=255)

                                          aaTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCaa                                               >  2:5165161/1‑93 (MQ=255)

                                           aTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAat                                              <  2:2459492/93‑1 (MQ=255)

                                           aTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAat                                              >  1:768582/1‑93 (MQ=255)

                                             gAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAatat                                            >  2:1270880/1‑93 (MQ=255)

                                             gAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAatat                                            >  2:5748528/1‑93 (MQ=255)

                                             gAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑CAGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAatat                                            >  2:279683/1‑93 (MQ=255)

                                               aaCGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTc                                          >  2:1379618/1‑93 (MQ=255)

                                               aaCGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTc                                          <  1:4559221/93‑1 (MQ=255)

                                               aaCGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTc                                          <  1:5536279/93‑1 (MQ=255)

                                                  gCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagc                                       >  1:4431287/1‑93 (MQ=255)

                                                  gCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagc                                       >  2:1184420/1‑93 (MQ=255)

                                                   cTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  2:1900602/93‑1 (MQ=255)

                                                   cTTTCCGCGATTTCTTTTATTCCCCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  2:2281497/93‑1 (MQ=255)

                                                    tttCCTCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagcag                                     <  2:5327372/93‑1 (MQ=255)

                                                      tCCGCTATTTCTTTTATTCCCCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGc                                   <  1:1236596/93‑1 (MQ=255)

                                                      tCCGCTATTTCTTTTATTCACCCTGCTCACGCTTTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGc                                   <  1:5893627/93‑1 (MQ=255)

                                                      tCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGc                                   <  2:110865/93‑1 (MQ=255)

                                                        cGCTATTTGTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGa                                 <  2:4716500/93‑1 (MQ=255)

                                                        cGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGa                                 >  2:874431/1‑93 (MQ=255)

                                                        cGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGa                                 >  2:6066798/1‑93 (MQ=255)

                                                          cTATTTCTTTTATTCCCCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAtt                               <  2:1548317/93‑1 (MQ=255)

                                                          cTATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAtt                               >  1:3367725/1‑93 (MQ=255)

                                                           tATTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGAAATTATCGCCGCAACCATTGGAAATATTCACAAGGCGATTa                              >  2:2691206/1‑93 (MQ=255)

                                                            aTTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGTGATTaa                             >  1:1806992/1‑93 (MQ=255)

                                                            aTTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTaa                             >  2:4887985/1‑93 (MQ=255)

                                                            aTTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTaa                             >  1:952504/1‑93 (MQ=255)

                                                            aTTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTaa                             >  2:4219888/1‑93 (MQ=255)

                                                            aTTTCTTTTATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTaa                             >  1:5831932/1‑93 (MQ=255)

                                                                tttttATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGAc                         <  2:3338090/92‑1 (MQ=255)

                                                                  tttATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGc                       >  1:2664266/1‑93 (MQ=255)

                                                                   ttATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCt                      >  2:3957506/1‑93 (MQ=255)

                                                                   ttATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCt                      >  1:1085790/1‑93 (MQ=255)

                                                                   ttATTCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCt                      <  1:4947251/93‑1 (MQ=255)

                                                                     tttCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTta                    <  1:4009930/92‑1 (MQ=255)

                                                                      ttCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtat                   >  2:3071449/1‑93 (MQ=255)

                                                                       tCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtata                  <  2:3432841/93‑1 (MQ=255)

                                                                       tCACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtata                  <  2:904613/93‑1 (MQ=255)

                                                                        cACCCTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATaa                 >  1:3807818/1‑93 (MQ=255)

                                                                         ccccTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAAc                <  1:1009546/92‑1 (MQ=255)

                                                                          cccTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAAcc               >  2:4250125/1‑93 (MQ=255)

                                                                          cccTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAAcc               >  2:4837141/1‑93 (MQ=255)

                                                                            cTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCt             >  1:2016167/1‑93 (MQ=255)

                                                                            cTGCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCt             >  1:3662650/1‑93 (MQ=255)

                                                                              gCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGa           <  1:1880383/93‑1 (MQ=255)

                                                                              gCTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGa           <  2:3539716/93‑1 (MQ=255)

                                                                               cTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAc          >  2:3222475/1‑93 (MQ=255)

                                                                               cTCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAc          <  2:561448/93‑1 (MQ=255)

                                                                                tCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAcc         >  1:3345855/1‑93 (MQ=255)

                                                                                tCACGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAcc         >  2:799764/1‑93 (MQ=255)

                                                                                  ccGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGt       <  2:3189821/92‑1 (MQ=255)

                                                                                  ccGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGt       <  2:1763389/92‑1 (MQ=255)

                                                                                  aCGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGt       >  2:2225879/1‑93 (MQ=255)

                                                                                   cGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTg      <  1:3491634/93‑1 (MQ=255)

                                                                                   cGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTg      <  1:209074/93‑1 (MQ=255)

                                                                                   cGCTGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTg      >  2:2115764/1‑93 (MQ=255)

                                                                                      tGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGc   <  1:5207663/93‑1 (MQ=255)

                                                                                      tGTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGc   >  2:602550/1‑93 (MQ=255)

                                                                                       gTCCACC‑GTTCCGGCCCAGGGCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTcggag  >  1:4366570/1‑91 (MQ=255)

                                                                                       gTCCACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCg  <  2:4421270/93‑1 (MQ=255)

AGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑C‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCG  >  NC_000913/4311404‑4311582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`