BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,435,684	+A	coding (400/501 nt)	ytfI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,435,681	1	.	A	100.0%	966.6 / NA	253	Q133K (CAA→AAA)	ytfI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (117/136); total (117/136)

Only 100 of 264 total aligned reads displayed.

GAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAT  >  NC_000913/4435589‑4435768

gAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac                                                                                          <  2:5982592/93‑1 (MQ=255)

 aGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑a                                                                                        >  2:4994294/1‑93 (MQ=255)

   gTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaa                                                                                      <  1:690651/93‑1 (MQ=255)

   gTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaa                                                                                      <  1:3903556/93‑1 (MQ=255)

     aGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaac                                                                                    <  2:1942322/93‑3 (MQ=255)

      gTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaaca                                                                                   <  1:1335372/93‑4 (MQ=255)

          aTTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACg                                                                                >  2:3665412/1‑93 (MQ=255)

           tttGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGa                                                                               >  1:4708563/1‑93 (MQ=255)

            ttGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGAt                                                                              <  2:1128047/93‑1 (MQ=255)

             tGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGAtt                                                                             <  2:3067617/93‑1 (MQ=255)

             tGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGAtt                                                                             <  1:271390/93‑1 (MQ=255)

                 tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTAt                                                                         <  1:5696761/93‑1 (MQ=255)

                 tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTAt                                                                         <  1:1707270/93‑1 (MQ=255)

                 tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTAt                                                                         >  2:4555258/1‑93 (MQ=255)

                 aacaaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTAt                                                                         <  2:1019816/89‑1 (MQ=255)

                  aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTAtc                                                                        >  2:1436578/1‑92 (MQ=255)

                  aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATg                                                                        >  2:3734738/1‑93 (MQ=255)

                    gaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGcc                                                                      <  2:4374321/93‑1 (MQ=255)

                     aagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCt                                                                     <  1:3379743/93‑1 (MQ=255)

                      agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTg                                                                    >  2:4472920/1‑93 (MQ=255)

                      agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTg                                                                    <  2:2128542/93‑1 (MQ=255)

                      agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTg                                                                    <  2:237172/93‑1 (MQ=255)

                      agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTg                                                                    <  2:1081668/93‑1 (MQ=255)

                       gaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTgg                                                                   <  1:3141185/93‑1 (MQ=255)

                         aaTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGa                                                                 <  1:1089276/93‑1 (MQ=255)

                         aaTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGa                                                                 <  2:5264415/93‑1 (MQ=255)

                          aTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGat                                                                <  1:4374475/93‑1 (MQ=255)

                            aCCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGatat                                                              >  1:2852155/1‑93 (MQ=255)

                            aCCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGatat                                                              >  2:747114/1‑93 (MQ=255)

                              ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTc                                                            >  1:5179786/1‑93 (MQ=255)

                               cATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCt                                                           <  1:4472271/93‑1 (MQ=255)

                                 tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTAc                                                         >  2:608841/1‑93 (MQ=255)

                                 tAACAATAAAGGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTAc                                                         <  1:947875/93‑1 (MQ=255)

                                  aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACa                                                        <  1:357164/93‑1 (MQ=255)

                                  aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACa                                                        >  2:815588/1‑93 (MQ=255)

                                    cAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGa                                                      >  2:1247015/1‑93 (MQ=255)

                                     aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaa                                                     >  1:3748826/1‑93 (MQ=255)

                                       tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaa                                                   <  1:3794982/93‑1 (MQ=255)

                                        aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaaa                                                  <  1:4689152/93‑1 (MQ=255)

                                        aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaaa                                                  >  2:2818476/1‑93 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 <  2:912819/93‑1 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 <  1:2212428/93‑1 (MQ=255)

                                          aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                >  2:4338787/1‑93 (MQ=255)

                                          aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                >  2:1542873/1‑93 (MQ=255)

                                           tGAATATAACTCATTCATGGATTTCAAAAACCGTTAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                               <  1:4156822/93‑1 (MQ=255)

                                            gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                              >  2:1744187/1‑93 (MQ=255)

                                            gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                              >  1:4528233/1‑93 (MQ=255)

                                            gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                              <  1:1880165/93‑1 (MQ=255)

                                              atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                            <  2:5246359/93‑1 (MQ=255)

                                              atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                            >  1:1842362/1‑93 (MQ=255)

                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           <  2:1677355/93‑1 (MQ=255)

                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           >  2:136960/1‑93 (MQ=255)

                                                 taACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                         <  1:2681151/93‑1 (MQ=255)

                                                 taACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                         >  1:2537983/1‑93 (MQ=255)

                                                   aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                       >  2:5113999/1‑93 (MQ=255)

                                                    cTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAt                                      >  2:4212896/1‑93 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTATAGAAAAAACAGTCTGACATa                                     >  2:1652384/1‑93 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     <  2:5621861/93‑1 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     <  2:4016289/93‑1 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     <  2:5830136/93‑1 (MQ=255)

                                                       attcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                   >  2:5369068/1‑93 (MQ=255)

                                                        ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                  <  2:3438762/93‑1 (MQ=255)

                                                         tcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGa                                 >  2:1871186/1‑93 (MQ=255)

                                                            tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                              <  2:3368372/93‑1 (MQ=255)

                                                            tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                              >  1:2313930/1‑93 (MQ=255)

                                                            tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                              >  1:4188463/1‑93 (MQ=255)

                                                             ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                             >  2:5309917/1‑93 (MQ=255)

                                                             ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                             <  1:5906822/93‑1 (MQ=255)

                                                              gATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg                            >  2:2426233/1‑93 (MQ=255)

                                                               aTTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa                           >  2:549998/1‑93 (MQ=255)

                                                                tttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt                          >  1:5258192/1‑93 (MQ=255)

                                                                  tCAAAAACCGTGAATTTCCGTTAGAACAAAACATACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa                        <  1:6018094/93‑1 (MQ=255)

                                                                   cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaa                       <  2:4700350/93‑1 (MQ=255)

                                                                    aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                      <  1:4301623/93‑1 (MQ=255)

                                                                    aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                      <  2:5773602/93‑1 (MQ=255)

                                                                     aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGACAAACCAGTCTGACATACCGAAAAGGATTAAAg                     >  2:1428735/1‑93 (MQ=255)

                                                                     aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                     <  1:1107008/93‑1 (MQ=255)

                                                                        aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGtt                  >  1:2165358/1‑93 (MQ=255)

                                                                          cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                <  2:1314711/93‑1 (MQ=255)

                                                                          cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                <  2:573873/93‑1 (MQ=255)

                                                                           gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGTGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata               <  2:5351671/93‑1 (MQ=255)

                                                                           gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata               >  2:832640/1‑93 (MQ=255)

                                                                           gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata               <  2:5562919/93‑1 (MQ=255)

                                                                            tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat              <  1:4792069/93‑1 (MQ=255)

                                                                              aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGTATTAAAGGTTATATTc            <  1:460667/93‑1 (MQ=255)

                                                                              aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTc            <  1:194106/93‑1 (MQ=255)

                                                                              aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTc            <  1:5640544/93‑1 (MQ=255)

                                                                               aTTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCa           <  2:1833251/93‑1 (MQ=255)

                                                                                tttCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc          >  1:5092286/1‑93 (MQ=255)

                                                                                  tCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa        <  2:3192462/93‑1 (MQ=255)

                                                                                  tCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa        <  1:1490896/93‑1 (MQ=255)

                                                                                   ccGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAg       <  1:5598938/93‑1 (MQ=255)

                                                                                   ccGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAg       <  1:3338554/93‑1 (MQ=255)

                                                                                      ttAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg    >  2:2853657/1‑93 (MQ=255)

                                                                                      ttAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg    >  2:1700094/1‑93 (MQ=255)

                                                                                       tAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAGAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGa   <  1:3304897/93‑1 (MQ=255)

                                                                                       tAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGa   >  2:2517422/1‑93 (MQ=255)

                                                                                       tAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGa   <  1:2464635/93‑1 (MQ=255)

                                                                                       tAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGa   >  1:2983026/1‑93 (MQ=255)

                                                                                        aGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAt  <  2:2649550/93‑1 (MQ=255)

GAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAT  >  NC_000913/4435589‑4435768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`