BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,748,385	A→G	D115D (GAT→GAC)	grpE ←	GrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,748,385	0	A	G	98.9%	680.0 / ‑4.7	189	D115D (GAT→GAC)	grpE	GrpE
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (92/95); total (93/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

AGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATATCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAA  >  NC_000913/2748295‑2748474

aGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTc                                                                                         <  2:633277/93‑1 (MQ=255)

 gTTTCGGCGATCACTTCAACGCCAATCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTcc                                                                                        <  1:5278446/93‑1 (MQ=255)

 gTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTcc                                                                                        <  2:2702858/93‑1 (MQ=255)

 gTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTcc                                                                                        <  1:3410366/93‑1 (MQ=255)

 gTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTcc                                                                                        <  2:5144352/93‑1 (MQ=255)

 gTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTcc                                                                                        <  1:2767307/93‑1 (MQ=255)

 gTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTcc                                                                                        >  1:5220429/1‑93 (MQ=255)

  tttCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCg                                                                                       >  2:4727545/1‑93 (MQ=255)

  tttCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCg                                                                                       <  1:2455051/93‑1 (MQ=255)

   ttCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCgg                                                                                      <  2:321317/93‑1 (MQ=255)

      ggCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGtt                                                                                   <  1:3237438/93‑1 (MQ=255)

      ggCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGtt                                                                                   >  1:1593403/1‑93 (MQ=255)

       gCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCTGGGTTa                                                                                  >  1:5432126/1‑93 (MQ=255)

       gCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTa                                                                                  <  2:5679917/93‑1 (MQ=255)

         gATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGc                                                                                <  2:5502753/93‑1 (MQ=255)

          aTCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCt                                                                               >  1:2524317/1‑93 (MQ=255)

            cACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCttt                                                                             >  2:3843356/1‑93 (MQ=255)

            cACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCttt                                                                             >  1:2685876/1‑93 (MQ=255)

              cTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTAt                                                                           <  2:412958/93‑1 (MQ=255)

              cTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTAt                                                                           <  2:1367166/93‑1 (MQ=255)

              cTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTAt                                                                           >  1:4077884/1‑93 (MQ=255)

               ttCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTAta                                                                          >  2:1475800/1‑92 (MQ=255)

               ttCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATc                                                                          >  1:2635865/1‑93 (MQ=255)

               ttCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATc                                                                          >  2:5440491/1‑93 (MQ=255)

               ttCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATc                                                                          >  2:3170546/1‑93 (MQ=255)

                tCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCa                                                                         <  1:4826998/93‑1 (MQ=255)

                tCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCa                                                                         <  2:2612899/93‑1 (MQ=255)

                tCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCa                                                                         <  1:2155271/93‑1 (MQ=255)

                 cAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAg                                                                        <  1:2816224/93‑1 (MQ=255)

                 cAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAg                                                                        <  1:4532388/93‑1 (MQ=255)

                   aCGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGcc                                                                      >  1:1827274/1‑93 (MQ=255)

                     gCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCAc                                                                    >  2:2807559/1‑93 (MQ=255)

                      ccAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACt                                                                   <  2:5023516/93‑1 (MQ=255)

                       cAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACtt                                                                  >  2:3985971/1‑93 (MQ=255)

                        aaaCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTc                                                                 >  1:2991391/1‑93 (MQ=255)

                        aaaCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTc                                                                 >  1:5666911/1‑93 (MQ=255)

                        aaaCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTc                                                                 <  1:5323339/93‑1 (MQ=255)

                         aaCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTcc                                                                <  2:357633/93‑1 (MQ=255)

                          aCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCa                                                               >  2:491966/1‑93 (MQ=255)

                           cTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAg                                                              <  2:2414427/93‑1 (MQ=255)

                            ttACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCTAgc                                                             >  2:4247900/1‑93 (MQ=255)

                            ttACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAgc                                                             <  1:121383/93‑1 (MQ=255)

                            ttACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAgc                                                             <  2:1533468/93‑1 (MQ=255)

                            tgaCGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAgc                                                             <  2:5887324/91‑1 (MQ=255)

                             tACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAgcg                                                            >  2:5631522/1‑93 (MQ=255)

                                gCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCAc                                                         <  1:3861134/93‑1 (MQ=255)

                                gCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCAc                                                         >  2:87673/1‑93 (MQ=255)

                                 cacaACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACg                                                        >  1:559018/1‑93 (MQ=255)

                                    aacaTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATc                                                     >  2:6103365/1‑93 (MQ=255)

                                     acaTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATcc                                                    <  1:2117529/93‑1 (MQ=255)

                                      caTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCa                                                   <  1:3584494/93‑1 (MQ=255)

                                       aTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAg                                                  >  2:4542426/1‑93 (MQ=255)

                                       aTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAg                                                  <  2:177594/93‑1 (MQ=255)

                                         ccAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGc                                                <  2:2925181/93‑1 (MQ=255)

                                           aGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTa                                              <  1:5436711/93‑1 (MQ=255)

                                              aTCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatca                                           >  2:208346/1‑93 (MQ=255)

                                                 gACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatcaatc                                        >  2:5382634/1‑93 (MQ=255)

                                                 gACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatcaatc                                        <  1:6135964/93‑1 (MQ=255)

                                                  aCTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatcaatca                                       <  1:1368617/93‑1 (MQ=255)

                                                   cTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCAc                                      <  2:1904623/93‑1 (MQ=255)

                                                   cTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCAc                                      >  1:3837163/1‑93 (MQ=255)

                                                    ttCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCAcc                                     <  2:3759667/93‑1 (MQ=255)

                                                    ttCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCAcc                                     <  2:1110698/93‑1 (MQ=255)

                                                    ttCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCAcc                                     <  1:5239706/93‑1 (MQ=255)

                                                    ttCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACATCCAGCGCACGATCCAGGCTATCAATCAcc                                     >  1:6010588/1‑93 (MQ=255)

                                                    ttCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGACACTTCAAGCGCACGATCAAGGCTATCAATCAcc                                     >  1:2270389/1‑93 (MQ=255)

                                                     tCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCg                                    >  2:3752300/1‑93 (MQ=255)

                                                       aGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGgc                                  >  2:3494450/1‑93 (MQ=255)

                                                        gCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGgca                                 <  1:719035/93‑1 (MQ=255)

                                                           tCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGgcagca                              >  2:4836280/1‑93 (MQ=255)

                                                            cAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCaa                             >  1:4153737/1‑93 (MQ=255)

                                                             cgCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAAt                            <  1:1958464/92‑1 (MQ=255)

                                                             aGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAAt                            <  2:2303995/93‑1 (MQ=255)

                                                             aGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAAt                            >  1:4926585/1‑93 (MQ=255)

                                                              gCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAAtt                           <  1:4224024/93‑1 (MQ=255)

                                                               cTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTc                          >  2:5963762/1‑93 (MQ=255)

                                                               cTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTc                          <  2:3058576/93‑1 (MQ=255)

                                                               cTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTc                          >  1:4510090/1‑93 (MQ=255)

                                                               cTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTc                          <  2:5238950/93‑1 (MQ=255)

                                                                tCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCg                         >  1:130980/1‑93 (MQ=255)

                                                                  aaggCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGtt                       <  1:596443/90‑1 (MQ=255)

                                                                  aaTGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGtt                       <  1:1015558/93‑1 (MQ=255)

                                                                  aaTGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGtt                       <  1:1319860/93‑1 (MQ=255)

                                                                  aaTGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGtt                       <  1:5039010/93‑1 (MQ=255)

                                                                   aTGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTtg                      >  1:2058554/1‑93 (MQ=255)

                                                                     gCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTtgat                    <  1:147063/93‑1 (MQ=255)

                                                                        ttCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGaa                 >  1:1171415/1‑93 (MQ=255)

                                                                        ttCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGaa                 >  1:2345313/1‑93 (MQ=255)

                                                                          cAACCATCGCAAACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAAtt               <  2:4689847/93‑1 (MQ=255)

                                                                           aaCCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAAttt              >  2:772893/1‑93 (MQ=255)

                                                                             ctaTCGCAGACATGTCCGGGTTAGCTTTATCATCCACTTACAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGACTTtct            >  1:2129155/3‑93 (MQ=255)

                                                                              cATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTtctc           <  2:1612400/93‑1 (MQ=255)

                                                                                tCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCa         >  2:5368119/1‑93 (MQ=255)

                                                                                tCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCa         <  2:1988593/93‑1 (MQ=255)

                                                                                tCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCa         >  2:2202632/1‑93 (MQ=255)

                                                                                acGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCa         <  2:1048587/92‑1 (MQ=255)

                                                                                  gCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAgc       <  2:2509534/93‑1 (MQ=255)

                                                                                    aGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAgcgc     <  2:1259305/93‑1 (MQ=255)

                                                                                     gACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGcgcg    >  2:5109699/1‑93 (MQ=255)

                                                                                       cATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGaa  >  1:915474/1‑93 (MQ=255)

AGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATATCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAA  >  NC_000913/2748295‑2748474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`