BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,446	G→C	G196G (GGC→GGG)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,446	0	G	C	98.0%	690.1 / 1.2	204	G196G (GGC→GGG)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (2/2); new base C (112/87); total (115/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 218 total aligned reads displayed.

CGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGA  >  NC_000913/2819356‑2819535

   gCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGcccccc                                                                                      <  1:1035976/93‑1 (MQ=255)

   gCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGcccccc                                                                                      <  1:1544929/93‑1 (MQ=255)

    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCcccccc                                                                                     >  2:3559274/1‑93 (MQ=255)

    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCcccccc                                                                                     >  1:1867091/1‑93 (MQ=255)

      aTCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCa                                                                                   >  1:3837972/1‑93 (MQ=255)

       tCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCaa                                                                                  >  2:5042519/1‑93 (MQ=255)

       tCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCaa                                                                                  >  2:3552641/1‑93 (MQ=255)

        cGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCaaa                                                                                 <  1:195495/93‑1 (MQ=255)

        cGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGATCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCaaa                                                                                 >  1:4277606/1‑93 (MQ=255)

            cTGGCGGTTGAACTGCATGAAGCCGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCCACTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgtg                                                                             <  1:2266609/93‑1 (MQ=255)

            cTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgtg                                                                             >  2:3797190/1‑93 (MQ=255)

            cTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgtg                                                                             >  1:941509/1‑93 (MQ=255)

             tGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTgg                                                                            >  2:1602989/1‑93 (MQ=255)

             tGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTgg                                                                            <  1:84513/93‑1 (MQ=255)

             tGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTgg                                                                            >  2:493231/1‑93 (MQ=255)

               gCGGTTGAACTGCATGAAGCCGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTc                                                                          <  1:5641324/93‑1 (MQ=255)

               gCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTc                                                                          >  1:6032623/1‑93 (MQ=255)

               gCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTc                                                                          <  2:2050684/93‑1 (MQ=255)

                 ggTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGc                                                                        >  1:2821012/1‑93 (MQ=255)

                 ggTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGc                                                                        >  2:1729386/1‑93 (MQ=255)

                  gTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGcc                                                                       <  2:6113158/93‑1 (MQ=255)

                   ttGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCg                                                                      >  1:841043/1‑93 (MQ=255)

                   ttGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCg                                                                      >  2:2162560/1‑93 (MQ=255)

                    tGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGt                                                                     <  2:4793077/93‑1 (MQ=255)

                      aaCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGa                                                                   >  1:134752/1‑93 (MQ=255)

                      aaCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGa                                                                   <  1:2746071/93‑1 (MQ=255)

                      aaCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGa                                                                   <  2:5083934/93‑1 (MQ=255)

                       aCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGAt                                                                  >  2:5874957/1‑93 (MQ=255)

                       aCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGAt                                                                  >  2:3839877/1‑93 (MQ=255)

                        cTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATc                                                                 >  1:680094/1‑93 (MQ=255)

                         tGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCg                                                                >  1:3133965/1‑93 (MQ=255)

                         tGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCg                                                                >  1:3293681/1‑93 (MQ=255)

                            aTGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTag                                                             >  1:594785/1‑93 (MQ=255)

                             tGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTaga                                                            >  1:21517/1‑93 (MQ=255)

                             tGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGACCCCCCCAAATGGGGTCGCCGTGATCGTaga                                                            >  1:3378732/1‑93 (MQ=255)

                              gAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaa                                                           >  1:2541860/1‑93 (MQ=255)

                                aGACGATGTTCCAGATCTCAGTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaaga                                                         <  2:2874411/93‑1 (MQ=255)

                                aGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCCAATGTGGTCGCCGTGATCGTagaagt                                                         >  1:5403811/1‑92 (MQ=255)

                                aGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaaga                                                         <  1:1337391/93‑1 (MQ=255)

                                 gACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAt                                                        >  1:5728295/1‑93 (MQ=255)

                                 gACAATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAt                                                        <  1:35469/93‑1 (MQ=255)

                                  aCGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAtt                                                       <  2:1949503/93‑1 (MQ=255)

                                  aCGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAtt                                                       >  1:3267397/1‑93 (MQ=255)

                                  aCGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAtt                                                       <  1:6106118/93‑1 (MQ=255)

                                    gATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTc                                                     >  2:4460751/1‑93 (MQ=255)

                                    gATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTc                                                     <  1:1143738/93‑1 (MQ=255)

                                    gATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTc                                                     <  1:3448795/93‑1 (MQ=255)

                                    gATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTc                                                     >  1:3978044/1‑93 (MQ=255)

                                       gTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGt                                                  <  2:648525/93‑1 (MQ=255)

                                        ttCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTg                                                 >  2:4251515/1‑93 (MQ=255)

                                         tCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGc                                                <  2:5625939/93‑1 (MQ=255)

                                          ccAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCa                                               <  2:5820554/93‑1 (MQ=255)

                                           cAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGCCCCCCCCCAAATGTGGTAGCCGTGATCGTAGAAGATTTCGGTGCAc                                              >  2:2971938/1‑93 (MQ=255)

                                             gATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACgg                                            >  2:3094773/1‑93 (MQ=255)

                                              aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                           >  1:2648223/1‑93 (MQ=255)

                                              aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                           <  2:5910584/93‑1 (MQ=255)

                                              aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                           >  2:5619621/1‑93 (MQ=255)

                                              aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                           >  1:3083768/1‑93 (MQ=255)

                                               tctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGc                                          <  2:1826133/93‑1 (MQ=255)

                                               tctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGc                                          <  2:2723714/93‑1 (MQ=255)

                                               tctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGc                                          >  2:5057745/1‑93 (MQ=255)

                                                 tcAGTGTAGCGGTCGCCTTCGTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCg                                        <  2:5698054/93‑1 (MQ=255)

                                                 tcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCg                                        >  1:5323887/1‑93 (MQ=255)

                                                   aaTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                      <  1:5252518/93‑1 (MQ=255)

                                                   aaTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAGTTCGGTGCACGGGCCGCa                                      >  1:9991/1‑93 (MQ=255)

                                                    aTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCAc                                     >  1:4548224/1‑93 (MQ=255)

                                                     tGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCCAAAGTGGTTGCCGGGGTCCGAGAAGATTTTGGTGCCACGGCCGCACg                                    >  1:3726710/1‑93 (MQ=255)

                                                       tAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGa                                  <  2:5121793/93‑1 (MQ=255)

                                                        aGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                 >  1:4766335/1‑93 (MQ=255)

                                                         gCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAcc                                <  2:458660/93‑1 (MQ=255)

                                                         gCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAcc                                <  2:4985438/93‑1 (MQ=255)

                                                          cGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCa                               <  2:1185314/93‑1 (MQ=255)

                                                          cGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCa                               >  1:4612417/1‑93 (MQ=255)

                                                            gTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgt                             <  2:4371308/93‑1 (MQ=255)

                                                               gCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCTCGGACCAGTGTc                          <  2:5544237/93‑1 (MQ=255)

                                                               gCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTc                          >  2:4525070/1‑93 (MQ=255)

                                                                 cGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAc                        <  2:2888675/93‑1 (MQ=255)

                                                                  gTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAcc                       >  1:5073455/1‑93 (MQ=255)

                                                                   tcttctTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAccc                      >  2:2641994/1‑93 (MQ=255)

                                                                    cttcttCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCAGGGGCCGCACGGACCAGTGGCACCCa                     >  1:1494057/1‑93 (MQ=255)

                                                                    cttcttCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCa                     >  2:5523857/1‑93 (MQ=255)

                                                                      tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCTGTGCACCGGCCGCCCGGACCAGTGTCCCCCacc                   >  1:4943927/1‑91 (MQ=255)

                                                                      tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGTCCGCACGGACCAGTGTCACCCATc                   <  1:3907185/93‑1 (MQ=255)

                                                                      tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATc                   <  2:4862840/93‑1 (MQ=255)

                                                                      tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATc                   <  2:5361555/93‑1 (MQ=255)

                                                                        ttcCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTg                 <  1:2212793/93‑1 (MQ=255)

                                                                        ttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTg                 >  2:3519256/1‑93 (MQ=255)

                                                                        ttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTg                 >  1:5212170/1‑93 (MQ=255)

                                                                            gggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAg             <  1:2793056/93‑1 (MQ=255)

                                                                             ggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGa            >  2:5187878/1‑93 (MQ=255)

                                                                             ggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGa            <  1:201266/93‑1 (MQ=255)

                                                                              gCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCCCCCATCTGCCAGaa           <  2:709375/93‑1 (MQ=255)

                                                                              gCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGaa           <  1:5662481/93‑1 (MQ=255)

                                                                                ttCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGt         <  1:3593226/93‑1 (MQ=255)

                                                                                ttCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGt         >  2:3397159/1‑93 (MQ=255)

                                                                                  cccGGGGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTg       <  1:4768209/93‑1 (MQ=255)

                                                                                     ggAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTCCCAGAAGTTGTCa    >  2:1486632/1‑93 (MQ=255)

                                                                                      gAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAg   >  2:3025530/1‑93 (MQ=255)

                                                                                       agcgCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGa  <  2:605734/90‑1 (MQ=255)

                                                                                       aGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGa  <  1:5503947/93‑1 (MQ=255)

CGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGA  >  NC_000913/2819356‑2819535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`