BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,823,915	A→G	K21E (AAG→GAG)	srlA →	PTS system, glucitol/sorbitol‑specific IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,823,915	0	A	G	100.0%	635.1 / NA	175	K21E (AAG→GAG)	srlA	PTS system, glucitol/sorbitol‑specific IIC component
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (108/67); total (108/67)

Only 100 of 181 total aligned reads displayed.

ATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAAAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAG  >  NC_000913/2823825‑2824004

ataaAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGagag                                                                                         <  2:4691929/93‑1 (MQ=255)

 taaAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAgg                                                                                        >  1:679142/1‑93 (MQ=255)

 taaAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAgg                                                                                        <  2:802277/93‑1 (MQ=255)

 taaAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAgg                                                                                        >  2:5091313/1‑93 (MQ=255)

 aaaaaCAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAgg                                                                                        <  1:5579607/92‑1 (MQ=255)

  aaaaCAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGgt                                                                                       <  2:4834134/93‑1 (MQ=255)

   aaaCAATAAATCCTGAAGGAGAGAACAATTATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGgtg                                                                                      <  2:1427072/93‑1 (MQ=255)

    aaCAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGgtgt                                                                                     >  2:4076691/1‑93 (MQ=255)

      cAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGttt                                                                                   <  2:5313121/93‑1 (MQ=255)

        aTAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTAc                                                                                 >  2:3642613/1‑93 (MQ=255)

         tAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTAcc                                                                                <  1:939566/93‑1 (MQ=255)

          aaaTCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCg                                                                               <  2:3738438/93‑1 (MQ=255)

            aTCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCggg                                                                             >  1:2074655/1‑93 (MQ=255)

             tCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGa                                                                            >  1:4105440/1‑93 (MQ=255)

             tCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGa                                                                            <  1:106930/93‑1 (MQ=255)

              ccTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGag                                                                           >  1:2164457/1‑92 (MQ=255)

               cTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATg                                                                          >  1:5717193/1‑93 (MQ=255)

                 gAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGt                                                                        >  2:3807190/1‑93 (MQ=255)

                    ggAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAc                                                                     >  1:1243813/1‑93 (MQ=255)

                    ggAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAc                                                                     >  1:1814400/1‑93 (MQ=255)

                    ggAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAc                                                                     <  2:3507031/93‑1 (MQ=255)

                       gagaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCgg                                                                  >  2:2989373/1‑93 (MQ=255)

                         gaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCa                                                                >  2:4628328/1‑93 (MQ=255)

                         gaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCa                                                                >  2:4708253/1‑93 (MQ=255)

                         gaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCa                                                                >  1:3980565/1‑93 (MQ=255)

                          aaCAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAt                                                               <  2:855804/93‑1 (MQ=255)

                          aaCAATGATAGAAACCATTACTCATGATGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAt                                                               >  1:2917551/1‑93 (MQ=255)

                           aCAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGGGTTTACGGGGTTGGGGACCGGCAtc                                                              >  1:2157280/1‑92 (MQ=255)

                            cAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGGGACCGGCAttc                                                             <  1:3970175/93‑1 (MQ=255)

                             aaTGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttct                                                            >  1:5876413/1‑93 (MQ=255)

                              aTGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttctt                                                           >  2:4260499/1‑93 (MQ=255)

                              aTGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttctt                                                           >  2:1740842/1‑93 (MQ=255)

                               tGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttcttc                                                          <  2:5747282/93‑1 (MQ=255)

                               tGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttcttc                                                          >  1:2307031/1‑93 (MQ=255)

                                  tAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGc                                                       >  1:4241575/1‑93 (MQ=255)

                                  tAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGc                                                       >  2:3847203/1‑93 (MQ=255)

                                    gAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTg                                                     >  1:625565/1‑93 (MQ=255)

                                    gAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTg                                                     >  1:5382804/1‑93 (MQ=255)

                                    gAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTg                                                     >  1:2485119/1‑93 (MQ=255)

                                     aaaCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGACATTCTTCCGCTGt                                                    <  1:77760/93‑1 (MQ=255)

                                     aaaCCATTACTCATGGTGCAGAGTGGTTTATCGAGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTACCGCTGt                                                    >  1:1565868/1‑93 (MQ=255)

                                       aCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTg                                                  >  1:3016795/1‑93 (MQ=255)

                                        ccATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTgt                                                 >  1:5128858/1‑92 (MQ=255)

                                        ccATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGa                                                 >  2:2701740/1‑93 (MQ=255)

                                        ccATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGa                                                 <  1:4758211/93‑1 (MQ=255)

                                        ccATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGa                                                 >  1:5281597/1‑93 (MQ=255)

                                         cATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGAt                                                >  1:563851/1‑93 (MQ=255)

                                         cATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGAt                                                >  2:5312926/1‑93 (MQ=255)

                                          aTTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGAtt                                               >  1:4541471/1‑93 (MQ=255)

                                          aTTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGAtt                                               <  2:1371235/93‑1 (MQ=255)

                                          aTTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTAACGGGATGGTGACCGGCATTCTTCCGCTGTTGAtt                                               >  1:3532165/1‑93 (MQ=255)

                                            tACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAg                                             <  2:4953788/93‑1 (MQ=255)

                                            tACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAg                                             >  2:742620/1‑93 (MQ=255)

                                             aCTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGc                                            >  1:1923377/1‑93 (MQ=255)

                                             aCTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGc                                            >  1:34295/1‑93 (MQ=255)

                                              cTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGcc                                           <  1:2044415/93‑1 (MQ=255)

                                               tCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCct                                          <  1:5871407/93‑1 (MQ=255)

                                                cATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACTGGCATTCTTCCGCTGTTGTTCACCctg                                         >  1:4347016/1‑93 (MQ=255)

                                                 aTGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgc                                        >  2:120478/1‑93 (MQ=255)

                                                 aTGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgc                                        >  1:2568987/1‑93 (MQ=255)

                                                  tGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgct                                       <  1:4205885/93‑1 (MQ=255)

                                                  tGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgct                                       <  1:5215864/93‑1 (MQ=255)

                                                    gTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTgg                                     <  1:2233779/93‑1 (MQ=255)

                                                    gTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTgg                                     <  2:5022726/93‑1 (MQ=255)

                                                     tGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGt                                    <  2:5455698/93‑1 (MQ=255)

                                                       cAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTa                                  <  1:3918957/93‑1 (MQ=255)

                                                         gagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATc                                <  2:6119773/93‑1 (MQ=255)

                                                         gagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATc                                <  1:6087276/93‑1 (MQ=255)

                                                           gTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAGTCTTCCGCTGTTGATTAGCCTGCTGGTTATCAt                              >  2:5926746/1‑93 (MQ=255)

                                                            tGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATg                             <  2:5685991/93‑1 (MQ=255)

                                                            tGGTTGATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATg                             <  2:3022843/93‑1 (MQ=255)

                                                              gTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGaa                           >  1:2659292/1‑93 (MQ=255)

                                                                ttATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACg                         <  1:3659920/93‑1 (MQ=255)

                                                                 tATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGc                        >  2:1730055/1‑93 (MQ=255)

                                                                 tATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGc                        >  2:2468006/1‑93 (MQ=255)

                                                                  aTCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCa                       >  1:2821708/1‑93 (MQ=255)

                                                                  aTCGGACTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAACATGAACGCa                       <  1:5457451/93‑1 (MQ=255)

                                                                   tCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCTGCTGTTGATTAGCCTGCTGGTTATCATGAACGCAc                      <  1:1156710/93‑1 (MQ=255)

                                                                   tCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCAc                      <  2:3416343/93‑1 (MQ=255)

                                                                    cGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACt                     <  1:6024801/93‑1 (MQ=255)

                                                                     gggCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTg                    >  1:3465567/1‑93 (MQ=255)

                                                                      ggCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTgt                   >  1:1848992/1‑92 (MQ=255)

                                                                       gCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGAt                  >  1:36968/1‑93 (MQ=255)

                                                                        cTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGAtt                 >  2:1590099/1‑93 (MQ=255)

                                                                          gTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTat               >  2:3300178/1‑92 (MQ=255)

                                                                          gTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTaa               >  1:5475779/1‑93 (MQ=255)

                                                                           ttCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAt              >  1:734200/1‑93 (MQ=255)

                                                                           ttCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAt              >  1:2028203/1‑93 (MQ=255)

                                                                            tCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAtt             >  1:4752293/1‑93 (MQ=255)

                                                                            tCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAtt             <  2:3930027/93‑1 (MQ=255)

                                                                             ccAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAttt            <  1:2688170/93‑1 (MQ=255)

                                                                               aaaaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTa          <  2:2092166/93‑1 (MQ=255)

                                                                                aaaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTAt         >  2:4703458/1‑93 (MQ=255)

                                                                                aaaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTAt         >  1:3920118/1‑93 (MQ=255)

                                                                                aaaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTAt         >  2:1575240/1‑93 (MQ=255)

                                                                                 aaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATc        <  1:4292478/93‑1 (MQ=255)

                                                                                 aaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATc        <  2:1338470/93‑1 (MQ=255)

                                                                                     gCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTc    >  2:4054079/1‑93 (MQ=255)

                                                                                       ggAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAg  >  2:5721130/1‑93 (MQ=255)

                                                                                       ggAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAg  >  2:1360930/1‑93 (MQ=255)

ATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAAAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAG  >  NC_000913/2823825‑2824004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`