BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,825,102	T→C	F230L (TTC→CTC)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,825,102	0	T	C	100.0%	722.0 / NA	199	F230L (TTC→CTC)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (115/84); total (115/84)

Only 100 of 209 total aligned reads displayed.

ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTA  >  NC_000913/2825012‑2825191

aTTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTttcctc                                                                                         <  1:4379743/93‑1 (MQ=255)

aTTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTttcctc                                                                                         >  1:1155252/1‑93 (MQ=255)

 ttGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCg                                                                                        >  2:2361739/1‑93 (MQ=255)

 ttGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCg                                                                                        >  2:1979102/1‑93 (MQ=255)

    cccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGcc                                                                                     >  2:3689075/1‑93 (MQ=255)

    cccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGcc                                                                                     >  1:2864705/1‑93 (MQ=255)

     ccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGccc                                                                                    >  1:3204456/1‑93 (MQ=255)

     ccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGccc                                                                                    <  1:4041482/93‑1 (MQ=255)

      cACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCa                                                                                   >  2:4181490/1‑93 (MQ=255)

       aCGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                                                  <  1:173481/93‑1 (MQ=255)

       aCGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                                                  >  1:4845912/1‑93 (MQ=255)

        cGGTCTTGCCCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAgg                                                                                 <  1:2035424/93‑1 (MQ=255)

         ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                                                >  2:1016843/1‑93 (MQ=255)

            cTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCa                                                                             >  1:853383/1‑93 (MQ=255)

             ttGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAg                                                                            <  1:1846876/93‑1 (MQ=255)

             ttGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAg                                                                            >  2:2484158/1‑93 (MQ=255)

               gCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGtt                                                                          <  1:3515691/93‑1 (MQ=255)

               gCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGtt                                                                          >  1:881011/1‑93 (MQ=255)

                cTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTa                                                                         >  1:20706/1‑93 (MQ=255)

                cTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTa                                                                         >  2:2649025/1‑93 (MQ=255)

                 tCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTAt                                                                        <  2:538588/93‑1 (MQ=255)

                 tCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTAt                                                                        >  2:630548/1‑93 (MQ=255)

                 cccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGACGCAGTTAt                                                                        <  1:4184294/92‑1 (MQ=255)

                  ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                                                       <  1:653051/93‑1 (MQ=255)

                   cGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCg                                                                      >  2:1314317/1‑93 (MQ=255)

                   cGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCg                                                                      <  1:2954377/93‑1 (MQ=255)

                      tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcac                                                                   <  2:3330490/93‑1 (MQ=255)

                      tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcac                                                                   >  1:2303669/1‑93 (MQ=255)

                      tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcac                                                                   >  1:738715/1‑93 (MQ=255)

                          gAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGt                                                               >  2:1561412/1‑93 (MQ=255)

                            gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                             <  2:2768800/93‑1 (MQ=255)

                              cATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                                           >  2:5330081/1‑93 (MQ=255)

                              cATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                                           >  1:5107624/1‑93 (MQ=255)

                               aTCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCg                                                          >  2:3946945/1‑93 (MQ=255)

                               aTCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCg                                                          >  1:5642534/1‑93 (MQ=255)

                                tCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCgg                                                         <  2:349879/93‑1 (MQ=255)

                                 ccACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGc                                                        <  1:338099/93‑1 (MQ=255)

                                    cTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTa                                                     <  1:4496253/93‑1 (MQ=255)

                                    cTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTa                                                     <  1:3356448/93‑1 (MQ=255)

                                     tGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                                                    <  1:3094351/93‑1 (MQ=255)

                                     tGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCAGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                                                    <  1:472805/93‑1 (MQ=255)

                                      tggTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTatt                                                   <  2:544715/92‑1 (MQ=255)

                                       ggTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattg                                                  >  1:2465385/1‑93 (MQ=255)

                                        gTCTTGGCATGCTGGCGCTCATGTGTTCCTTCCCACGGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattga                                                 <  1:5166845/93‑1 (MQ=255)

                                         gcTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgat                                                <  2:1860603/92‑1 (MQ=255)

                                           tGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                                              <  2:1450930/93‑1 (MQ=255)

                                             gTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGc                                            <  2:1629322/93‑1 (MQ=255)

                                              tCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCg                                           <  1:1600031/93‑1 (MQ=255)

                                               cATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGt                                          <  2:5647861/93‑1 (MQ=255)

                                               cATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGt                                          >  2:4448103/1‑93 (MQ=255)

                                               cATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGt                                          <  1:1086638/93‑1 (MQ=255)

                                                 tGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGc                                        >  1:2578947/1‑93 (MQ=255)

                                                  tcTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTCTCGGCGTATTGATTGGCGTGCa                                       <  2:1189460/92‑1 (MQ=255)

                                                   gtGGCGCTCATCTGTTCCTTCCCACTTCTTTCACCTTTCCTCCGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAg                                      <  1:3264968/92‑1 (MQ=255)

                                                    tGGCGCTTATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGa                                     <  2:2307849/93‑1 (MQ=255)

                                                     ggCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                                    <  1:5747096/93‑1 (MQ=255)

                                                      gcgcTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt                                   <  1:3570106/93‑1 (MQ=255)

                                                       cgcTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTg                                  <  1:1114377/93‑1 (MQ=255)

                                                       cgcTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTg                                  >  1:3614817/1‑93 (MQ=255)

                                                        gcTCATCTGTTCCTTCCCACTGCTTTCCCCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTgg                                 <  2:4584842/93‑1 (MQ=255)

                                                        gcTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCCGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTAGCGTGCAGATTgg                                 >  1:3246726/1‑93 (MQ=255)

                                                         cTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGt                                >  2:410713/1‑93 (MQ=255)

                                                         cTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGt                                >  1:5063255/1‑93 (MQ=255)

                                                          tCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtc                               >  2:1792724/1‑93 (MQ=255)

                                                          tCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtc                               <  1:559075/93‑1 (MQ=255)

                                                          tCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtc                               >  2:2019925/1‑93 (MQ=255)

                                                          tCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtc                               <  1:1249466/93‑1 (MQ=255)

                                                          tCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtc                               >  2:5055748/1‑93 (MQ=255)

                                                          tCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGACCACGCGCAGTTATCGCACAGGTTATCAGAGTATTGATTGGCGTGCAAAATGGtc                               >  1:1558947/1‑93 (MQ=255)

                                                           cATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtct                              >  2:2985624/1‑93 (MQ=255)

                                                             tCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                            >  2:3391918/1‑93 (MQ=255)

                                                               tGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGc                          >  1:6114360/1‑93 (MQ=255)

                                                                gTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCa                         >  1:255540/1‑93 (MQ=255)

                                                                 ttccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCaa                        <  1:1361488/93‑1 (MQ=255)

                                                                  tccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAat                       >  2:10548/1‑93 (MQ=255)

                                                                   ccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGACGTGCAGATTGGTCTCGGCAata                      >  1:3495147/1‑93 (MQ=255)

                                                                    cttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAatat                     <  2:5694058/93‑1 (MQ=255)

                                                                    cttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAatat                     >  1:5341345/1‑93 (MQ=255)

                                                                    cttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAatat                     >  1:2529591/1‑93 (MQ=255)

                                                                      tccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGCATTGATTGGCGTGCAGATTGGTCTCGGCAATATTc                   >  2:3139654/1‑93 (MQ=255)

                                                                        ccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccg                 >  2:383613/1‑93 (MQ=255)

                                                                        ccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccg                 >  2:7376/1‑93 (MQ=255)

                                                                        ccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccg                 >  1:6009009/1‑93 (MQ=255)

                                                                         cACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgc                >  2:882455/1‑93 (MQ=255)

                                                                           cTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgccg              >  1:3306225/1‑93 (MQ=255)

                                                                             gCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGGTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCa            >  1:2256117/1‑93 (MQ=255)

                                                                               tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATc          >  1:3028912/1‑93 (MQ=255)

                                                                                 tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTg        >  2:4853231/1‑93 (MQ=255)

                                                                                 tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTg        <  1:350808/93‑1 (MQ=255)

                                                                                 tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTg        >  1:4782483/1‑93 (MQ=255)

                                                                                    ccTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCt     >  2:5268376/1‑93 (MQ=255)

                                                                                    ccTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCt     >  2:6023073/1‑93 (MQ=255)

                                                                                     cTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCATTATTCCCCCGCATCTGGCtt    >  2:4692494/1‑93 (MQ=255)

                                                                                     cTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCtt    <  2:3436600/93‑1 (MQ=255)

                                                                                      tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt   >  2:2581563/1‑93 (MQ=255)

                                                                                      tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt   >  2:5965147/1‑93 (MQ=255)

                                                                                      tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt   <  2:4517340/93‑1 (MQ=255)

                                                                                      tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCCTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt   >  1:1489205/1‑93 (MQ=255)

                                                                                       ttcctcGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGGTTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTa  >  2:2543629/1‑93 (MQ=255)

                                                                                       ttcctcGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTa  <  1:2622211/93‑1 (MQ=255)

ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTA  >  NC_000913/2825012‑2825191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`