BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,924	A→G	L294L (TTA→TTG)	lysR →	LysR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,924	0	A	G	99.1%	733.3 / ‑2.9	212	L294L (TTA→TTG)	lysR	LysR
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (95/115); total (97/115)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 218 total aligned reads displayed.

GGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTA  >  NC_000913/2977834‑2978013

ggTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGcc                                                                                         <  2:5410004/93‑1 (MQ=255)

 gTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCg                                                                                        >  1:4391234/1‑93 (MQ=255)

    ccGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGaaa                                                                                     <  1:5027741/93‑1 (MQ=255)

    ccGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGaaa                                                                                     <  2:3255452/93‑1 (MQ=255)

     cGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAAc                                                                                    <  1:2522930/93‑1 (MQ=255)

     cGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAAc                                                                                    <  2:280239/93‑1 (MQ=255)

      gTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACt                                                                                   >  2:4442318/1‑93 (MQ=255)

       ttCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTg                                                                                  >  1:2228246/1‑93 (MQ=255)

        tCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTgg                                                                                 >  2:3707963/1‑93 (MQ=255)

         cACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGt                                                                                <  2:5773554/93‑1 (MQ=255)

         cACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGt                                                                                <  2:5749659/93‑1 (MQ=255)

         cACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGt                                                                                <  2:1812918/93‑1 (MQ=255)

         cACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGt                                                                                >  2:4067689/1‑93 (MQ=255)

          aCCGTCAGCGTGATCCTCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTc                                                                               <  1:2632049/93‑1 (MQ=255)

           ccGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCa                                                                              <  2:6137138/93‑1 (MQ=255)

             gTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACt                                                                            <  2:3921531/93‑1 (MQ=255)

             gTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACt                                                                            <  1:4560604/93‑1 (MQ=255)

             gTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACt                                                                            >  1:5878218/1‑93 (MQ=255)

             gTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACt                                                                            >  2:700397/1‑93 (MQ=255)

              tCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACtt                                                                           <  1:3684598/93‑1 (MQ=255)

               cAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTtc                                                                          <  1:2575082/93‑1 (MQ=255)

                 gCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTtctc                                                                        >  1:3326137/1‑93 (MQ=255)

                 gCCTGATCCGCCCCCTGAACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTtctc                                                                        >  2:4418429/1‑93 (MQ=255)

                  ccTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTctct                                                                       <  1:3672930/93‑1 (MQ=255)

                  ccTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTctct                                                                       >  1:2182229/1‑93 (MQ=255)

                   cTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCtt                                                                      <  2:3475899/93‑1 (MQ=255)

                    tGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTg                                                                     >  2:330219/1‑93 (MQ=255)

                    tGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTg                                                                     >  2:2619478/1‑93 (MQ=255)

                    tGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTg                                                                     >  1:4355521/1‑93 (MQ=255)

                     gATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGa                                                                    >  2:3987999/1‑93 (MQ=255)

                        tcGCCCCCTTCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGTGTTGCCGAAACTGGTCACTTCTCTTGACGc                                                                 <  2:2058334/92‑1 (MQ=255)

                        ccGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGc                                                                 >  1:4428413/1‑93 (MQ=255)

                         cGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCt                                                                >  1:471018/1‑93 (MQ=255)

                           cccccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAt                                                              >  1:5268031/1‑93 (MQ=255)

                           cccccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGAAGCTAt                                                              >  1:1475276/1‑93 (MQ=255)

                             cccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAttt                                                            <  1:3770001/93‑1 (MQ=255)

                             cccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAttt                                                            >  2:1674900/1‑93 (MQ=255)

                              ccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtttt                                                           <  2:4003852/93‑1 (MQ=255)

                               cTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTg                                                          >  2:2379035/1‑93 (MQ=255)

                               cTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTg                                                          <  1:739550/93‑1 (MQ=255)

                                  cACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtcg                                                       >  2:2770802/1‑93 (MQ=255)

                                     cGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCa                                                    <  2:1657343/93‑1 (MQ=255)

                                      gCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAg                                                   <  2:4578961/93‑1 (MQ=255)

                                       cccGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGc                                                  >  1:2784433/1‑93 (MQ=255)

                                         cGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTa                                                >  1:4772268/1‑93 (MQ=255)

                                         cGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTa                                                <  1:1845179/93‑1 (MQ=255)

                                         cGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTa                                                <  2:5162164/93‑1 (MQ=255)

                                          gTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTAc                                               <  2:5977153/93‑1 (MQ=255)

                                          gTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTAc                                               <  2:5935705/93‑1 (MQ=255)

                                          gTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCGAGTTAGTCGTCAGCTAc                                               >  1:5485252/1‑93 (MQ=255)

                                          gTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGCTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTAc                                               <  2:2871678/93‑1 (MQ=255)

                                          gTCATCAGCGCTGGTCCAGGGGTTTAGGGGGAATTTAAAACGGGGGTGGCCGAAATGGGTCACTTTCCTTGCCGCATTTTGGTGGTCGGCTAc                                               >  2:1725124/1‑93 (MQ=255)

                                           tcatcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTTGTCAGCTACg                                              >  1:1563956/1‑93 (MQ=255)

                                           tcatcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACg                                              <  1:2104856/93‑1 (MQ=255)

                                            catcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGa                                             >  2:5447148/1‑93 (MQ=255)

                                            catcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGa                                             <  2:3614504/93‑1 (MQ=255)

                                             atcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAc                                            >  2:5135613/1‑93 (MQ=255)

                                              tcaGCGCTGGTTCAGGCGTTTATTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           <  2:1494715/93‑1 (MQ=255)

                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           >  2:1706645/1‑93 (MQ=255)

                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           <  2:1252013/93‑1 (MQ=255)

                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           >  1:3564210/1‑93 (MQ=255)

                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           <  1:4822052/93‑1 (MQ=255)

                                                aGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGc                                         <  2:2706597/93‑1 (MQ=255)

                                                aGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGc                                         <  2:3059187/93‑1 (MQ=255)

                                                 gcgcTGGTTCAGGCGTTTAGCGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCa                                        >  1:3062361/1‑93 (MQ=255)

                                                  cgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCAt                                       >  2:4347217/1‑93 (MQ=255)

                                                    cTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaa                                     >  2:4609433/1‑93 (MQ=255)

                                                    cTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaa                                     <  1:5975326/93‑1 (MQ=255)

                                                     tGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaaa                                    >  1:1709833/1‑93 (MQ=255)

                                                     tGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGCTGCCGAAACTGGCCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACGGCATaaa                                    >  1:2005604/1‑93 (MQ=255)

                                                      ggTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaaaa                                   <  2:3840490/93‑1 (MQ=255)

                                                        ttCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGc                                 <  1:3156148/93‑1 (MQ=255)

                                                            ggCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACa                             <  1:5344105/93‑1 (MQ=255)

                                                            ggCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACa                             <  2:3145824/93‑1 (MQ=255)

                                                              cGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGc                           <  1:625582/93‑1 (MQ=255)

                                                                tttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCAt                         <  1:5516969/93‑1 (MQ=255)

                                                                tttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCAt                         >  2:384528/1‑93 (MQ=255)

                                                                tttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCAt                         <  2:2813378/93‑1 (MQ=255)

                                                                 ttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATc                        <  2:920069/93‑1 (MQ=255)

                                                                 ttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATc                        <  1:3987870/93‑1 (MQ=255)

                                                                     tGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCg                    >  2:1363452/1‑93 (MQ=255)

                                                                      gggCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCgg                   >  2:1334591/1‑93 (MQ=255)

                                                                      gggCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCgg                   <  1:5638859/93‑1 (MQ=255)

                                                                       ggCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGc                  <  2:3518966/93‑1 (MQ=255)

                                                                        gCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCa                 >  1:6056274/1‑93 (MQ=255)

                                                                         cATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCAt                <  2:2070841/93‑1 (MQ=255)

                                                                           tttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATgg              <  2:570138/93‑1 (MQ=255)

                                                                           tttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATgg              >  2:4911232/1‑93 (MQ=255)

                                                                            ttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGa             <  1:1270510/93‑1 (MQ=255)

                                                                             tACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGAt            <  1:5053903/93‑1 (MQ=255)

                                                                             tACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGAt            <  2:5852613/93‑1 (MQ=255)

                                                                              tcAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGGCGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATc           <  2:838334/92‑1 (MQ=255)

                                                                               cAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcg          >  1:1143124/1‑93 (MQ=255)

                                                                               cAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcg          >  1:1106409/1‑93 (MQ=255)

                                                                                aaGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgc         <  1:638005/93‑1 (MQ=255)

                                                                                  gCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgccg       <  1:856109/93‑1 (MQ=255)

                                                                                   gggggTTGCCAAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgccgc      <  1:2435441/92‑1 (MQ=255)

                                                                                     gggTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCgg    <  1:1851442/93‑1 (MQ=255)

                                                                                       gTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTa  <  2:117308/93‑1 (MQ=255)

                                                                                       gTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTa  >  2:773004/1‑93 (MQ=255)

GGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTA  >  NC_000913/2977834‑2978013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`