BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	234,824	G→A	P9P (CCG→CCA)	yafS →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	234,824	0	G	A	99.1%	816.4 / ‑3.7	216	P9P (CCG→CCA)	yafS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (124/90); total (124/92)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 217 total aligned reads displayed.

AAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCT  >  NC_000913/234734‑234913

aaaCCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGc                                                                                         >  2:1329343/1‑93 (MQ=255)

aaaCCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGc                                                                                         <  1:3380237/93‑1 (MQ=255)

 aaCCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCa                                                                                        >  1:5744781/1‑93 (MQ=255)

  aCCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCaa                                                                                       <  2:3064784/93‑1 (MQ=255)

   cccAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAag                                                                                      <  2:3313224/93‑1 (MQ=255)

    ccAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAaga                                                                                     >  1:623094/1‑93 (MQ=255)

    ccAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAaga                                                                                     <  2:4477447/93‑1 (MQ=255)

    ccAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAaga                                                                                     <  2:2609498/93‑1 (MQ=255)

    ccAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAaga                                                                                     >  2:3235635/1‑93 (MQ=255)

       gATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTc                                                                                  >  1:3396397/1‑93 (MQ=255)

       gATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTc                                                                                  <  2:2292606/93‑1 (MQ=255)

         tGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTccc                                                                                <  2:4171178/93‑1 (MQ=255)

          gTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCt                                                                               <  2:4446361/93‑1 (MQ=255)

           tAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTc                                                                              <  1:1029230/93‑1 (MQ=255)

           tAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTc                                                                              <  1:1920770/93‑1 (MQ=255)

             aTTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCaa                                                                            <  1:3642523/93‑1 (MQ=255)

               tGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAAc                                                                          <  1:5010716/93‑1 (MQ=255)

                gTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACt                                                                         <  1:5555072/93‑1 (MQ=255)

                 tcatcaAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTg                                                                        >  1:3170159/1‑93 (MQ=255)

                   atcaAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTc                                                                      <  1:1588967/93‑1 (MQ=255)

                   atcaAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTc                                                                      >  2:1153496/1‑93 (MQ=255)

                    tcaAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCg                                                                     >  2:4969934/1‑93 (MQ=255)

                     caAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGt                                                                    >  1:3131852/1‑93 (MQ=255)

                       aaGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTgg                                                                  >  2:3769632/1‑93 (MQ=255)

                        aGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGc                                                                 <  2:3628740/93‑1 (MQ=255)

                           cGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTcc                                                              <  2:6022710/93‑1 (MQ=255)

                            gggAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCt                                                             >  2:3275708/1‑93 (MQ=255)

                            gggAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCt                                                             >  2:1836667/1‑93 (MQ=255)

                            gggAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCt                                                             <  1:1289160/93‑1 (MQ=255)

                             ggAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTg                                                            >  1:4425855/1‑93 (MQ=255)

                             ggAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTg                                                            <  1:5440584/93‑1 (MQ=255)

                             ggAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTg                                                            >  2:1897082/1‑93 (MQ=255)

                             ggAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTg                                                            >  2:1044472/1‑93 (MQ=255)

                              gAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGa                                                           <  2:2653691/93‑1 (MQ=255)

                                aTACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGAtt                                                         >  1:2108384/1‑93 (MQ=255)

                                 tACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTg                                                        <  1:1656320/93‑1 (MQ=255)

                                 tACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTg                                                        >  2:3132288/1‑93 (MQ=255)

                                 tACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTg                                                        >  2:2412581/1‑93 (MQ=255)

                                 tACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTg                                                        >  2:1299/1‑93 (MQ=255)

                                  aCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGc                                                       <  1:2860991/93‑1 (MQ=255)

                                  aCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGc                                                       <  1:5188664/93‑1 (MQ=255)

                                   cTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGcg                                                      >  1:1850623/1‑92 (MQ=255)

                                   cTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCt                                                      >  1:2748112/1‑93 (MQ=255)

                                   cTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCt                                                      <  2:2021597/93‑1 (MQ=255)

                                   cTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCt                                                      >  2:4948589/1‑93 (MQ=255)

                                     gTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTgg                                                    <  1:5374980/93‑1 (MQ=255)

                                      ttAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTggg                                                   <  1:2489050/93‑1 (MQ=255)

                                      ttAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTggg                                                   <  2:1627926/93‑1 (MQ=255)

                                      ttAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTggg                                                   >  2:2680450/1‑93 (MQ=255)

                                       tAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTgggg                                                  <  1:2355207/93‑1 (MQ=255)

                                       tAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTgggg                                                  >  1:2672942/1‑93 (MQ=255)

                                        aaGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGc                                                 >  2:4143705/1‑93 (MQ=255)

                                           aTTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGAt                                              >  1:4189578/1‑93 (MQ=255)

                                            ttCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGAtt                                             >  1:756888/1‑93 (MQ=255)

                                            ttCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGAtt                                             >  2:4715048/1‑93 (MQ=255)

                                             tCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGAttt                                            <  1:72332/93‑1 (MQ=255)

                                              cATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTg                                           >  1:5986371/1‑93 (MQ=255)

                                              cATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTg                                           >  1:6024634/1‑93 (MQ=255)

                                              cATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTg                                           >  1:4843542/1‑93 (MQ=255)

                                               aTAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGc                                          >  1:4314448/1‑93 (MQ=255)

                                                tAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGcc                                         >  2:5834352/1‑93 (MQ=255)

                                                tAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGcc                                         >  2:5721373/1‑93 (MQ=255)

                                                 aGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGccc                                        <  2:5321419/93‑1 (MQ=255)

                                                   aTTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTg                                      <  2:4755247/93‑1 (MQ=255)

                                                     tACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTggg                                    >  1:3481326/1‑93 (MQ=255)

                                                      aCCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTgggg                                   >  1:220829/1‑93 (MQ=255)

                                                      aCCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCCTGGCTCCTGATTGCTGGGGCGATTTGCCCTgggg                                   <  2:815761/93‑1 (MQ=255)

                                                       ccTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGa                                  >  2:5576592/1‑93 (MQ=255)

                                                       ccTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGa                                  <  1:354634/93‑1 (MQ=255)

                                                         tctcAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGaaa                                >  1:901581/1‑93 (MQ=255)

                                                          ctcAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAg                               >  1:5252411/1‑93 (MQ=255)

                                                           tcAGTGTGAAACGGAAGGTTGTGATGAAGCCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGc                              >  2:5263402/1‑93 (MQ=255)

                                                           tcAGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGc                              <  2:2203869/93‑1 (MQ=255)

                                                             aGTGTGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCtt                            >  2:3975647/1‑93 (MQ=255)

                                                              gtgtGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCttt                           <  2:2884345/93‑1 (MQ=255)

                                                              gtgtGAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCttt                           <  2:54494/93‑1 (MQ=255)

                                                                tggAAGCGGAAGGTTGTGATGAAACCAGCAAGAGTTCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTAt                         <  2:3368391/91‑1 (MQ=255)

                                                                 tgAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATc                        <  2:2234066/93‑1 (MQ=255)

                                                                 tgAAACGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATc                        >  2:2269459/1‑93 (MQ=255)

                                                                   aaaCGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGc                      >  2:3944578/1‑93 (MQ=255)

                                                                    aaCGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCa                     >  2:4317920/1‑93 (MQ=255)

                                                                     aCGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCaa                    <  2:509616/93‑1 (MQ=255)

                                                                     aCGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCaa                    <  1:3107611/93‑1 (MQ=255)

                                                                      cGGAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAg                   >  1:4008129/1‑93 (MQ=255)

                                                                       ggAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAgg                  >  2:3672723/1‑93 (MQ=255)

                                                                       ggAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAgg                  <  2:1799513/93‑1 (MQ=255)

                                                                        gAAGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGgc                 <  2:5510733/93‑1 (MQ=255)

                                                                         aaGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGgcg                >  1:1379083/1‑93 (MQ=255)

                                                                         aaGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGgcg                <  2:2516959/93‑1 (MQ=255)

                                                                          aGGTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCCAGgcgc               >  1:2920520/1‑93 (MQ=255)

                                                                           ggTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCt              <  2:3706031/93‑1 (MQ=255)

                                                                            gTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTg             >  2:3223160/1‑93 (MQ=255)

                                                                            gTTGTGATGAAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTg             >  2:848844/1‑93 (MQ=255)

                                                                                tgatgaAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAgc         >  2:3002981/1‑93 (MQ=255)

                                                                                 gatgaAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAgcg        >  1:3964979/1‑93 (MQ=255)

                                                                                  atgaAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAgcgc       <  1:145652/93‑1 (MQ=255)

                                                                                   tgaAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGcc      >  2:4774776/1‑93 (MQ=255)

                                                                                   tgaAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGcc      >  2:1196101/1‑93 (MQ=255)

                                                                                    gaAACCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCa     >  2:6137509/1‑93 (MQ=255)

                                                                                       aCCAGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCt  >  2:460267/1‑93 (MQ=255)

AAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCT  >  NC_000913/234734‑234913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`