BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,185,776	T→C	F121L (TTC→CTC)	yqiG →	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,185,776	0	T	C	100.0%	918.1 / NA	249	F121L (TTC→CTC)	yqiG	putative membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (121/128); total (121/128)

Only 100 of 257 total aligned reads displayed.

GGATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGG  >  NC_000913/3185686‑3185865

ggATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTAtctc                                                                                         >  2:5172222/1‑93 (MQ=255)

 gATTTACTGCTGAATTTATTGAAAAACCTCAGCTAACCCTGCTCGATGGTTGTTACCCTATCGAAAGGGAACAACAAATTCCAACTTATCTCg                                                                                        <  1:5476155/93‑1 (MQ=255)

 gATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCg                                                                                        <  1:664045/93‑1 (MQ=255)

 gATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCg                                                                                        <  1:2134969/93‑1 (MQ=255)

   tttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGAt                                                                                      >  2:5193842/1‑93 (MQ=255)

   tttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGAt                                                                                      <  1:4326114/93‑1 (MQ=255)

    ttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATa                                                                                     >  1:569291/1‑93 (MQ=255)

    ttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATa                                                                                     <  2:317680/93‑1 (MQ=255)

    ttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATa                                                                                     >  2:4865361/1‑93 (MQ=255)

     tACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACCAATTACAACTTATCTCGATaa                                                                                    >  1:225584/1‑93 (MQ=255)

      tttgaggaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGATAAAGAAAAACAAACTACAATTTATCTCGATaaa                                                                                   >  2:5478241/3‑93 (MQ=255)

       cTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAg                                                                                  <  2:5639596/93‑1 (MQ=255)

       cTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAg                                                                                  <  1:2356251/93‑1 (MQ=255)

        tgatgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAgg                                                                                 <  1:4646442/93‑1 (MQ=255)

         gatgaATTTATTGAAAAAACTTAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAggg                                                                                >  1:4014398/1‑93 (MQ=255)

         gatgaATTTATTGAAAAAACTCAGCAAAACCTGGTCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAggg                                                                                >  1:4940014/1‑93 (MQ=255)

         gatgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAggg                                                                                >  1:245303/1‑93 (MQ=255)

         gatgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAggg                                                                                >  1:5613514/1‑93 (MQ=255)

          atgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAAGTACAACTTATCTCGATAAAGGGa                                                                               >  1:3487493/1‑93 (MQ=255)

           tgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGaa                                                                              <  2:495862/93‑1 (MQ=255)

            gaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGaaa                                                                             <  1:601325/93‑1 (MQ=255)

             aaTTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGaaaa                                                                            >  2:815312/1‑93 (MQ=255)

              aTTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGaaaag                                                                           >  1:4061040/1‑92 (MQ=255)

              aTTTATTGAAAAAACTCAGCAAAACCTGATCGATGATTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAAt                                                                           >  1:6084330/1‑93 (MQ=255)

               tttATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATg                                                                          >  1:5436660/1‑93 (MQ=255)

                 tATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAGAATAAAAACAAATTACAACTTATCTCGATTAAGGGAAAATGCa                                                                        >  1:4232733/1‑93 (MQ=255)

                 tATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCa                                                                        >  1:4853065/1‑93 (MQ=255)

                 tATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCa                                                                        >  1:2675491/1‑93 (MQ=255)

                   ttGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAAt                                                                      >  2:895956/1‑93 (MQ=255)

                     gAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTa                                                                    <  2:2289459/93‑1 (MQ=255)

                     gAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTa                                                                    <  2:398186/93‑1 (MQ=255)

                      aaaaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTAt                                                                   >  2:6036638/1‑93 (MQ=255)

                       aaaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATc                                                                  <  2:1239034/93‑1 (MQ=255)

                       aaaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATc                                                                  <  1:1550652/93‑1 (MQ=255)

                       aaaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATc                                                                  <  1:4600081/93‑1 (MQ=255)

                        aaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATcc                                                                 >  1:4199666/1‑93 (MQ=255)

                          aaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCat                                                               >  1:2946028/1‑93 (MQ=255)

                          aaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCat                                                               >  2:3514612/1‑93 (MQ=255)

                           aCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCata                                                              <  1:1682423/93‑1 (MQ=255)

                            cTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCatat                                                             >  2:459844/1‑93 (MQ=255)

                             tCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCatatc                                                            >  2:375674/1‑92 (MQ=255)

                               aGCAAAACCTGATCGATGGTTGTGACCCTATCGAAAAAAAAAAACAAATTACAACTTATCTCGATAAATGTAAAATGGAATTATCCATATCTg                                                          >  1:1910575/1‑93 (MQ=255)

                                 cAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCa                                                        <  2:1096480/93‑1 (MQ=255)

                                  aaaaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCAc                                                       <  2:5501010/93‑1 (MQ=255)

                                  aaaaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCAc                                                       >  2:3903622/1‑93 (MQ=255)

                                     aCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTc                                                    >  1:5528466/1‑93 (MQ=255)

                                     aCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTc                                                    <  2:6137659/93‑1 (MQ=255)

                                      ccTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCa                                                   <  2:1753033/93‑1 (MQ=255)

                                      ccTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCa                                                   >  2:680944/1‑93 (MQ=255)

                                      ccTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCa                                                   <  2:5063920/93‑1 (MQ=255)

                                       cTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAg                                                  <  2:5699707/93‑1 (MQ=255)

                                       cTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAg                                                  >  1:1822948/1‑93 (MQ=255)

                                        tGATCGATGGTTGTTACCATATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAgg                                                 <  1:3335153/93‑1 (MQ=255)

                                          aTCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCa                                               <  1:2151545/93‑1 (MQ=255)

                                             gATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATgg                                            <  2:594836/93‑1 (MQ=255)

                                                   tgttACCCTATCGAAAAAGAAAAACAAATTACATCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaaaa                                      <  2:1598520/93‑1 (MQ=255)

                                                   tgttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaaaa                                      <  1:2650019/93‑1 (MQ=255)

                                                   tgttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaaaa                                      <  1:3023856/93‑1 (MQ=255)

                                                   tgttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaaaa                                      <  2:2015498/93‑1 (MQ=255)

                                                    gttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAAt                                     >  1:1975430/1‑93 (MQ=255)

                                                       aCCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACa                                  <  1:1464425/93‑1 (MQ=255)

                                                       aCCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACa                                  >  1:3673828/1‑93 (MQ=255)

                                                         ccTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACaaa                                >  2:4061315/1‑93 (MQ=255)

                                                         ccTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACaaa                                >  2:3457678/1‑93 (MQ=255)

                                                           tATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGa                              <  2:2552899/93‑1 (MQ=255)

                                                           tATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGa                              <  2:3377190/93‑1 (MQ=255)

                                                            aTCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGAt                             <  2:2802306/93‑1 (MQ=255)

                                                             tCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATg                            >  1:4490127/1‑93 (MQ=255)

                                                              cGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGc                           >  2:2460078/1‑93 (MQ=255)

                                                              cGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGc                           >  2:5872437/1‑93 (MQ=255)

                                                               gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                          >  1:1135979/1‑93 (MQ=255)

                                                               gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                          >  2:578506/1‑93 (MQ=255)

                                                               gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                          <  2:4661516/93‑1 (MQ=255)

                                                                aaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCaa                         <  1:4957344/93‑1 (MQ=255)

                                                                  aaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAAc                       >  2:1471576/1‑93 (MQ=255)

                                                                  aaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAAc                       >  1:3645349/1‑93 (MQ=255)

                                                                   aagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACt                      >  2:366490/1‑93 (MQ=255)

                                                                   aagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTGAAAATACAAAGATGCAAACt                      <  1:5933469/93‑1 (MQ=255)

                                                                    agaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTg                     >  2:3011373/1‑93 (MQ=255)

                                                                      aaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGa                   <  1:1749893/93‑1 (MQ=255)

                                                                       aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                  >  2:4099982/1‑93 (MQ=255)

                                                                       aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                  >  1:1530459/1‑93 (MQ=255)

                                                                       aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                  >  1:4720366/1‑93 (MQ=255)

                                                                       aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                  >  1:4357864/1‑93 (MQ=255)

                                                                          aCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcc               <  2:335113/93‑1 (MQ=255)

                                                                          aCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcc               >  1:3742557/1‑93 (MQ=255)

                                                                           cAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcct              <  2:4230725/93‑1 (MQ=255)

                                                                           cAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcct              >  1:1644047/1‑93 (MQ=255)

                                                                            aaaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctc             <  1:1000044/93‑1 (MQ=255)

                                                                             aaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcc            >  1:3202839/1‑93 (MQ=255)

                                                                              aTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcct           <  1:5650614/93‑1 (MQ=255)

                                                                               ttACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTg          <  1:5785758/93‑1 (MQ=255)

                                                                                 aCAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGaa        >  2:747769/1‑93 (MQ=255)

                                                                                   aaCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACt      >  2:5257925/1‑93 (MQ=255)

                                                                                   aaCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACt      >  1:3163549/1‑93 (MQ=255)

                                                                                   aaCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACt      >  2:4001183/1‑93 (MQ=255)

                                                                                    aCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACtt     >  1:2827854/1‑93 (MQ=255)

                                                                                    aCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACtt     >  1:5306394/1‑93 (MQ=255)

                                                                                     cTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACttt    <  1:1348759/93‑1 (MQ=255)

                                                                                       tATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTgg  >  1:2460755/1‑93 (MQ=255)

GGATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGG  >  NC_000913/3185686‑3185865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`