Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,537,252 C→T P744S (CCG→TCG)  b3407 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,537,2520CT96.3% 769.7 / 9.0 216P744S (CCG→TCG) b3407hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base T (120/88);  total (125/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-01

Only 100 of 225 total aligned reads displayed.

AACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGT  >  NC_000913/3537160‑3537341
                                                                                            |                                                                                         
aaCGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGcc                                                                                           <  2:4485226/93‑1 (MQ=255)
  cGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCg                                                                                         <  2:2009808/93‑1 (MQ=255)
   gTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCgg                                                                                        >  2:1761165/1‑93 (MQ=255)
       cGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGc                                                                                    >  2:1351769/1‑93 (MQ=255)
        gCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAACACAACCGCTCGGCAGcc                                                                                   >  1:3388006/1‑93 (MQ=255)
        gCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGcc                                                                                   >  2:1154758/1‑93 (MQ=255)
         cccTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCa                                                                                  <  2:2538225/93‑1 (MQ=255)
         cccTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCa                                                                                  >  2:3046492/1‑93 (MQ=255)
          ccTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCaa                                                                                 >  2:5948029/1‑93 (MQ=255)
           cTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGTCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCaaa                                                                                >  1:3963215/1‑93 (MQ=255)
            tGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAAc                                                                               >  2:3136463/1‑93 (MQ=255)
              aCTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCa                                                                             >  1:4944243/1‑93 (MQ=255)
              aCTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCa                                                                             <  1:5138122/93‑1 (MQ=255)
              aCTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCa                                                                             >  2:4994579/1‑93 (MQ=255)
              aCTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCa                                                                             >  2:5862392/1‑93 (MQ=255)
               cTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCAc                                                                            <  1:2140716/93‑1 (MQ=255)
                taggcgcCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCAcg                                                                           <  2:1345002/90‑1 (MQ=255)
                tATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACTCCCGCAAAACAACCGCTCGGCAGCCAAACCAcc                                                                           >  2:3763661/1‑92 (MQ=255)
                tATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCAcg                                                                           <  1:4212742/93‑1 (MQ=255)
                  tGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCAcgcg                                                                         >  1:1180570/1‑93 (MQ=255)
                    cgcCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGt                                                                       <  1:4860366/93‑1 (MQ=255)
                    cgcCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGt                                                                       <  2:1329810/93‑1 (MQ=255)
                      ccTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCg                                                                     >  1:5986880/1‑93 (MQ=255)
                      ccTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCg                                                                     >  1:2560542/1‑93 (MQ=255)
                        tGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTg                                                                   <  1:5594953/93‑1 (MQ=255)
                        tGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTg                                                                   >  2:1425985/1‑93 (MQ=255)
                        tGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTg                                                                   >  2:3031500/1‑93 (MQ=255)
                         ggATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGa                                                                  <  2:4602389/93‑1 (MQ=255)
                             gAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAgcg                                                              >  1:4788963/1‑93 (MQ=255)
                              agcagcCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAgcgc                                                             >  2:5141628/1‑93 (MQ=255)
                               gcagcCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCa                                                            <  2:2991383/93‑1 (MQ=255)
                                  gcCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGcc                                                         >  1:2937948/1‑93 (MQ=255)
                                    cTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTg                                                       >  2:4777345/1‑93 (MQ=255)
                                    cTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTg                                                       >  1:4440787/1‑93 (MQ=255)
                                     tGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGc                                                      >  1:4646088/1‑93 (MQ=255)
                                         gAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGt                                                  >  2:4123103/1‑93 (MQ=255)
                                         gAAACCAACGCTCGTCGCGGCGGCGGTAATGAACACCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCgga                                                  >  2:3584488/1‑92 (MQ=255)
                                            aCCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAAt                                               >  1:1811419/1‑93 (MQ=255)
                                            aCCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAAt                                               >  2:2914382/1‑93 (MQ=255)
                                             ccAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATa                                              >  2:5240636/1‑93 (MQ=255)
                                             ccAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATa                                              >  1:1568028/1‑93 (MQ=255)
                                             ccAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATa                                              >  2:2158088/1‑93 (MQ=255)
                                             ccAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATa                                              <  1:1149413/93‑1 (MQ=255)
                                              cAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAg                                             <  2:1858369/93‑1 (MQ=255)
                                               aaCGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAgc                                            <  1:2800099/93‑1 (MQ=255)
                                                 cGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAgcgc                                          <  2:5057324/93‑1 (MQ=255)
                                                  gCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGcgcg                                         >  1:1673614/1‑93 (MQ=255)
                                                  gCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGcgcg                                         >  2:169777/1‑93 (MQ=255)
                                                   cTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCga                                        <  2:5434169/93‑1 (MQ=255)
                                                    tcgtcgCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCgat                                       >  1:4140192/1‑93 (MQ=255)
                                                    tcgtcgCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCgat                                       >  2:6029659/1‑93 (MQ=255)
                                                     cgtcgCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCgatg                                      <  2:2073202/93‑1 (MQ=255)
                                                     cgtcgCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCgatg                                      >  1:219924/1‑93 (MQ=255)
                                                      gtcgCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCgatga                                     <  1:3782285/93‑1 (MQ=255)
                                                        cgcgGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGatgatg                                   <  2:3949186/93‑1 (MQ=255)
                                                         gcggcggcgGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATgatgg                                  >  1:3318382/1‑93 (MQ=255)
                                                         gcggcggcgGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATgatgg                                  >  1:540611/1‑93 (MQ=255)
                                                          cggcggcggTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATgatgga                                 <  1:1409028/93‑1 (MQ=255)
                                                          cggcggcggTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATgatgga                                 <  2:5710484/93‑1 (MQ=255)
                                                           ggcggcggTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATgatggat                                <  1:5783559/93‑1 (MQ=255)
                                                              ggcggTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATgcg                             >  1:1023051/1‑93 (MQ=255)
                                                               gcggTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATgcgc                            <  2:3307906/93‑1 (MQ=255)
                                                               gcggTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATgcgc                            >  1:4379348/1‑93 (MQ=255)
                                                                  gTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTgg                         <  2:4645468/93‑1 (MQ=255)
                                                                   tAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggc                        <  2:1054717/93‑1 (MQ=255)
                                                                   tAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggc                        >  2:617134/1‑93 (MQ=255)
                                                                   tAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggc                        >  1:4237184/1‑93 (MQ=255)
                                                                    aaTGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcg                       >  2:859263/1‑93 (MQ=255)
                                                                     aTGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcgg                      <  2:3641797/93‑1 (MQ=255)
                                                                      tGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggg                     >  2:2269347/1‑92 (MQ=255)
                                                                      tGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggc                     >  1:3049461/1‑93 (MQ=255)
                                                                      tGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggc                     >  2:4884547/1‑93 (MQ=255)
                                                                       gAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcg                    >  1:4431794/1‑93 (MQ=255)
                                                                       gAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcg                    >  1:369657/1‑93 (MQ=255)
                                                                       gAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcg                    >  1:5314875/1‑93 (MQ=255)
                                                                        aaCGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcgg                   >  2:2377539/1‑93 (MQ=255)
                                                                        aaCGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcgg                   >  1:3352019/1‑93 (MQ=255)
                                                                          cGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCa                 >  1:4518241/1‑93 (MQ=255)
                                                                            cccGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAAt               <  1:3850002/93‑1 (MQ=255)
                                                                             ccGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATg              >  2:886996/1‑93 (MQ=255)
                                                                             ccGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATg              >  1:1908006/1‑93 (MQ=255)
                                                                              cGCAACACAACCGCTCATCAACCAAACCGCGCGGTCGTGAAGCACAGCCTGCCGGTAATAGCGCCATGATGGATGCGCTGGCAGCGGCAATgg             <  2:5875180/93‑1 (MQ=255)
                                                                              cGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATgg             <  2:3548211/93‑1 (MQ=255)
                                                                              cGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATgg             >  1:2457848/1‑93 (MQ=255)
                                                                              cGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATgg             >  1:3540772/1‑93 (MQ=255)
                                                                                 aaaaGAACCGCTCGGCAGCAAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCa          <  1:3831826/93‑1 (MQ=255)
                                                                                 aaaaCAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCa          >  2:1144106/1‑93 (MQ=255)
                                                                                 aaaaCAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCa          >  1:722331/1‑93 (MQ=255)
                                                                                  aaaCAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaa         >  1:1285302/1‑93 (MQ=255)
                                                                                   aacaacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaa        >  1:2952665/1‑93 (MQ=255)
                                                                                   aacaacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaa        >  1:2892074/1‑93 (MQ=255)
                                                                                   aacaacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaa        >  2:4570264/1‑93 (MQ=255)
                                                                                    acaacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaa       <  2:2036513/93‑1 (MQ=255)
                                                                                    acaacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaa       >  2:706657/1‑93 (MQ=255)
                                                                                     caacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaaa      <  1:1993195/93‑1 (MQ=255)
                                                                                      ccccGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaaaa     <  2:1603920/91‑1 (MQ=255)
                                                                                      aacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaaaa     <  1:4830686/93‑1 (MQ=255)
                                                                                       acCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAAc    >  1:5845155/1‑93 (MQ=255)
                                                                                       acCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAAc    >  2:2798749/1‑93 (MQ=255)
                                                                                         cGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGt  >  1:5679053/1‑93 (MQ=255)
                                                                                            |                                                                                         
AACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGT  >  NC_000913/3537160‑3537341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑