BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,736,298	T→C	N391N (AAT→AAC)	malS →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,736,298	0	T	C	99.6%	884.2 / ‑5.0	249	N391N (AAT→AAC)	malS	alpha‑amylase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (129/119); total (130/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01

Only 100 of 261 total aligned reads displayed.

TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTA  >  NC_000913/3736208‑3736387

tAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAccc                                                                                         <  1:1233900/93‑1 (MQ=255)

tAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAccc                                                                                         >  1:299575/1‑93 (MQ=255)

tAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAccc                                                                                         <  1:273377/93‑1 (MQ=255)

tAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAccc                                                                                         <  2:3538323/93‑1 (MQ=255)

 aaCGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCt                                                                                        >  1:2519572/1‑93 (MQ=255)

 aaCGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCt                                                                                        <  2:2965516/93‑1 (MQ=255)

   cGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTgg                                                                                      >  2:1457226/1‑93 (MQ=255)

    gATTACATTAATGTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGa                                                                                     <  1:5788373/93‑1 (MQ=255)

     aTTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGAt                                                                                    >  2:2307141/1‑93 (MQ=255)

       tACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTc                                                                                  <  1:3567412/93‑1 (MQ=255)

       tACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTc                                                                                  <  1:529430/93‑1 (MQ=255)

        aCATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcg                                                                                 >  2:4613646/1‑93 (MQ=255)

        aCATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcg                                                                                 >  2:4924736/1‑93 (MQ=255)

        aCATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcg                                                                                 >  1:5187576/1‑93 (MQ=255)

         cATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTCCGACAACCCTGGATTcga                                                                                <  1:655211/93‑1 (MQ=255)

         cATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcga                                                                                >  2:5065629/1‑93 (MQ=255)

         cATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcga                                                                                >  2:779372/1‑93 (MQ=255)

         cATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcga                                                                                >  1:621400/1‑93 (MQ=255)

          aTTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgag                                                                               >  1:3100675/1‑92 (MQ=255)

          aTTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgac                                                                               >  1:1022140/1‑93 (MQ=255)

            tAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgacga                                                                             <  2:5118088/93‑1 (MQ=255)

               tttCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGAtcc                                                                          >  2:298874/1‑92 (MQ=255)

                    gCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTa                                                                     <  1:6073244/93‑1 (MQ=255)

                    gCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTa                                                                     <  1:4613689/93‑1 (MQ=255)

                    gCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTa                                                                     >  2:2062429/1‑93 (MQ=255)

                    gCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTa                                                                     <  2:5378982/93‑1 (MQ=255)

                      gACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATg                                                                   <  1:2014239/93‑1 (MQ=255)

                      gACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATg                                                                   >  1:1124785/1‑93 (MQ=255)

                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  >  1:968337/1‑93 (MQ=255)

                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  >  2:1165945/1‑93 (MQ=255)

                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  <  2:1139316/93‑1 (MQ=255)

                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  >  1:4047433/1‑93 (MQ=255)

                          aaaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGc                                                               <  2:4157432/93‑1 (MQ=255)

                           aaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCt                                                              >  2:3605561/1‑93 (MQ=255)

                           aaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCt                                                              >  2:4401796/1‑93 (MQ=255)

                            aCAGGCTGGGATAAATGGTGGGGAAAAAACTTGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTa                                                             <  1:1831691/93‑1 (MQ=255)

                              aGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGc                                                           >  2:3656801/1‑93 (MQ=255)

                               ggCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGcc                                                          <  1:4716909/93‑1 (MQ=255)

                                 cTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGATGATCTCACTATGTCGCTAGCCtt                                                        >  2:1200760/1‑93 (MQ=255)

                                 cTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCtt                                                        >  2:712361/1‑93 (MQ=255)

                                 cTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCAATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCtt                                                        <  1:5762704/93‑1 (MQ=255)

                                   gggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCtttt                                                      >  2:5824550/1‑93 (MQ=255)

                                    ggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCttttt                                                     <  1:2845829/93‑1 (MQ=255)

                                    ggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCttttt                                                     <  1:4817756/93‑1 (MQ=255)

                                    ggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCttttt                                                     <  2:2893612/93‑1 (MQ=255)

                                    ggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCttttt                                                     <  2:3446266/93‑1 (MQ=255)

                                      aTAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGc                                                   >  1:37200/1‑93 (MQ=255)

                                       tAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGcc                                                  >  2:4463766/1‑93 (MQ=255)

                                        aaaTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCg                                                 >  2:571974/1‑93 (MQ=255)

                                         aaTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCgg                                                <  2:4689309/93‑1 (MQ=255)

                                         aaTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCgg                                                >  2:550691/1‑93 (MQ=255)

                                         aaTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTCCCgg                                                >  1:3289201/1‑93 (MQ=255)

                                              tggGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATc                                           >  2:2838651/1‑93 (MQ=255)

                                               ggggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCa                                          <  2:5651866/93‑1 (MQ=255)

                                                gggAAAAAACTGGATCAGAACGGATATCGTCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaa                                         <  1:3022123/93‑1 (MQ=255)

                                                gggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaa                                         <  1:1657441/93‑1 (MQ=255)

                                                gggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaa                                         <  1:4267190/93‑1 (MQ=255)

                                                  gAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaaaa                                       >  2:3489354/1‑93 (MQ=255)

                                                  gAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaaaa                                       <  2:4041466/93‑1 (MQ=255)

                                                  gAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaaaa                                       >  2:4376178/1‑93 (MQ=255)

                                                   aaaaaaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAAc                                      >  1:2401449/1‑93 (MQ=255)

                                                    aaaaaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAAcc                                     >  1:549907/1‑93 (MQ=255)

                                                      aaaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGa                                   <  2:25808/93‑1 (MQ=255)

                                                       aaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGaa                                  <  1:166829/93‑1 (MQ=255)

                                                        aCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAAt                                 >  1:5052817/1‑93 (MQ=255)

                                                        aCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAAt                                 <  2:3141267/93‑1 (MQ=255)

                                                         cTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATc                                >  1:670311/1‑93 (MQ=255)

                                                          tGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCa                               >  1:2141128/1‑93 (MQ=255)

                                                           ggATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCaa                              >  1:499097/1‑93 (MQ=255)

                                                           ggATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCaa                              <  1:2762560/93‑1 (MQ=255)

                                                           ggATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCaa                              >  1:3333365/1‑93 (MQ=255)

                                                           ggATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCaa                              <  2:3243125/93‑1 (MQ=255)

                                                            gATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAAc                             >  2:6048764/1‑93 (MQ=255)

                                                             aTCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCACACCCGCATCAACt                            >  2:876415/1‑93 (MQ=255)

                                                             aTCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACt                            <  1:4312760/93‑1 (MQ=255)

                                                                 gAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCg                        <  2:1732202/93‑1 (MQ=255)

                                                                     ggATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCttt                    >  2:2204134/1‑92 (MQ=255)

                                                                     ggATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTc                    <  2:3679868/93‑1 (MQ=255)

                                                                     ggATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTc                    >  1:1691763/1‑93 (MQ=255)

                                                                      gATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCt                   >  1:1910245/1‑93 (MQ=255)

                                                                      gATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCt                   >  1:4118107/1‑93 (MQ=255)

                                                                      gATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCt                   <  2:1317888/93‑1 (MQ=255)

                                                                        tatCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTgg                 >  1:567295/1‑93 (MQ=255)

                                                                          tCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTc               >  2:2118563/1‑93 (MQ=255)

                                                                          tCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTc               <  1:1299532/93‑1 (MQ=255)

                                                                          tCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTc               >  2:2007658/1‑93 (MQ=255)

                                                                          tCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTc               >  1:806467/1‑93 (MQ=255)

                                                                          tCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTc               <  1:5897239/93‑1 (MQ=255)

                                                                            ggCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTg             >  1:4338647/1‑93 (MQ=255)

                                                                             gCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGc            <  2:1776548/93‑1 (MQ=255)

                                                                              cGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGcc           <  2:245319/93‑1 (MQ=255)

                                                                              cGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGcc           <  1:3835623/93‑1 (MQ=255)

                                                                               gATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCg          >  2:4841318/1‑93 (MQ=255)

                                                                                aTTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCgg         >  2:3833016/1‑93 (MQ=255)

                                                                                  tACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtg       <  2:1552101/93‑1 (MQ=255)

                                                                                   aCGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtgt      <  1:379318/93‑1 (MQ=255)

                                                                                     gACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTc    <  1:143575/93‑1 (MQ=255)

                                                                                     gACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTc    >  2:4331612/1‑93 (MQ=255)

                                                                                     gACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTc    <  2:4227614/93‑1 (MQ=255)

                                                                                       cAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCta  <  1:3436683/93‑1 (MQ=255)

TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTA  >  NC_000913/3736208‑3736387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`