BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,765,203	A→T	Q119L (CAG→CTG)	yibJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,765,203	0	A	T	98.0%	165.9 / NA	50	Q119L (CAG→CTG)	yibJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (25/24); minor base G (0/1); total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC  >  NC_000913/3765184‑3765292

cGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCg                  >  2:5728399/1‑93 (MQ=9)

 gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 <  2:2406783/93‑1 (MQ=12)

 gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 >  1:1015056/1‑93 (MQ=12)

 gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 >  2:1551705/1‑93 (MQ=12)

  cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                <  2:3226363/93‑1 (MQ=16)

  cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                >  2:4116259/1‑93 (MQ=255)

   tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               >  2:4198454/1‑93 (MQ=255)

   tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               <  2:2672141/93‑1 (MQ=255)

   tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               >  1:826424/1‑93 (MQ=255)

   tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               >  1:2253177/1‑93 (MQ=255)

    attatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGTGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCa              <  2:3825174/93‑1 (MQ=11)

     ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             <  2:307340/93‑1 (MQ=255)

     ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             >  2:1877967/1‑93 (MQ=255)

     ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             >  1:2352516/1‑93 (MQ=255)

     ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             <  1:5554556/93‑1 (MQ=255)

      tatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa            >  1:1577886/1‑93 (MQ=255)

      tatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa            <  2:313104/93‑1 (MQ=255)

      tatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa            >  1:3425773/1‑93 (MQ=255)

      tatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa            >  1:1577887/1‑93 (MQ=255)

       atGGCCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           <  2:1847053/93‑1 (MQ=255)

       atGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           <  1:1932376/93‑1 (MQ=255)

       atGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           >  2:1493085/1‑93 (MQ=255)

       atGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           <  2:456886/93‑1 (MQ=255)

        tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:2258871/93‑1 (MQ=255)

        tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  1:1822487/93‑1 (MQ=255)

        tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:5816045/93‑1 (MQ=255)

        tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          >  1:3909788/1‑93 (MQ=255)

        tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  1:376328/93‑1 (MQ=255)

         gACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAt         >  1:1611827/1‑93 (MQ=255)

         gACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAt         >  2:209237/1‑93 (MQ=255)

          aCCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATg        <  2:4110017/93‑1 (MQ=255)

           cccGCTGCTGGGTCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt       <  2:2876585/93‑1 (MQ=255)

           cccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt       >  1:3268822/1‑93 (MQ=255)

            ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      >  1:2779498/1‑93 (MQ=255)

            ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      >  1:3090721/1‑93 (MQ=255)

            ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      >  1:3083752/1‑93 (MQ=255)

            ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      <  2:3156771/93‑1 (MQ=255)

            ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      >  1:3054957/1‑93 (MQ=255)

            ccGCTGCGGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      <  1:3553604/93‑1 (MQ=255)

             cGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     <  2:1742578/93‑1 (MQ=255)

             cGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     <  1:779696/93‑1 (MQ=255)

             cGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     <  1:4321246/93‑1 (MQ=255)

              gctgctGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    <  2:306766/93‑1 (MQ=255)

              gctgctGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    <  1:5252966/93‑1 (MQ=255)

               ctgctgGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt   <  2:4148134/93‑1 (MQ=255)

                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:389063/93‑1 (MQ=255)

                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  >  1:5345533/1‑93 (MQ=255)

                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:4405198/93‑1 (MQ=255)

                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  >  2:4496857/1‑93 (MQ=255)

                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  1:4635593/93‑1 (MQ=255)

                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:4797144/93‑1 (MQ=255)

                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  >  1:1715137/1‑93 (MQ=255)

                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:6101815/93‑1 (MQ=255)

                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  >  2:736231/1‑93 (MQ=255)

CGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC  >  NC_000913/3765184‑3765292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`