BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,810,753	T→C	A132A (GCT→GCC)	b3639 →	Dfp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,810,753	0	T	C	99.1%	808.5 / ‑2.6	229	A132A (GCT→GCC)	b3639	Dfp
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (118/109); total (119/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 247 total aligned reads displayed.

GGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTC  >  NC_000913/3810663‑3810844

ggCGAAGGACCTGGTATCGAGGATTGGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCcgccgc                                                                                           <  1:5425428/93‑1 (MQ=255)

  cGAATGCCCTGGTATCGCCGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCa                                                                                         <  2:1085528/93‑1 (MQ=255)

  cGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCa                                                                                         <  2:83743/93‑1 (MQ=255)

   gAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCAc                                                                                        <  1:3545213/93‑1 (MQ=255)

   gAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCAc                                                                                        >  1:3928471/1‑93 (MQ=255)

   gAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCAc                                                                                        <  1:5147970/93‑1 (MQ=255)

    aaTGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACg                                                                                       >  2:3533695/1‑93 (MQ=255)

    aaTGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACg                                                                                       >  2:5301779/1‑93 (MQ=255)

     aTGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACgc                                                                                      >  2:4641425/1‑93 (MQ=255)

      tGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACgca                                                                                     <  1:1303680/93‑1 (MQ=255)

      agACCTTGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACgca                                                                                     <  1:5244085/92‑1 (MQ=255)

         ccTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACgcagca                                                                                  >  2:3587147/1‑93 (MQ=255)

         ccTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACgcagca                                                                                  >  1:2405605/1‑93 (MQ=255)

          cTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCAt                                                                                 >  1:1776595/1‑93 (MQ=255)

          cTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCAt                                                                                 >  2:1532414/1‑93 (MQ=255)

          cTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCAt                                                                                 >  2:5383745/1‑93 (MQ=255)

           tGGTATCGAGTATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATa                                                                                <  1:4634614/93‑1 (MQ=255)

           tGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATa                                                                                <  2:4046574/93‑1 (MQ=255)

           tGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATa                                                                                >  1:1206220/1‑93 (MQ=255)

            ggTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATaa                                                                               <  1:1055904/93‑1 (MQ=255)

            ggTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATaa                                                                               <  1:1130241/93‑1 (MQ=255)

             gTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAAt                                                                              <  2:3756961/93‑1 (MQ=255)

               aTCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAAttt                                                                            >  1:375583/1‑93 (MQ=255)

               aTCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAAttt                                                                            <  2:749382/93‑1 (MQ=255)

               aTCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAAttt                                                                            <  1:4896157/93‑1 (MQ=255)

                tCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTa                                                                           >  2:3344512/1‑93 (MQ=255)

                tCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTa                                                                           >  1:338035/1‑93 (MQ=255)

                 cgacgaTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTag                                                                          >  2:2202879/1‑93 (MQ=255)

                 cgacgaTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGGACCGTGCCGCCGCCACGCAGCATAATTTag                                                                          >  1:485129/1‑93 (MQ=255)

                  gacgaTTTGCCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTaga                                                                         <  1:1092046/93‑1 (MQ=255)

                   gcgttttGTCTGGCTCCACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTagag                                                                        <  1:4763016/89‑1 (MQ=255)

                   acgaTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTagag                                                                        >  2:5600397/1‑93 (MQ=255)

                    cgaTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAgg                                                                       <  2:3984874/93‑1 (MQ=255)

                    cgaTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAgg                                                                       <  2:4345130/93‑1 (MQ=255)

                     gaTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGGACCGTGCGGCCGCCAGGCAGCATAGTTTAGAggg                                                                      >  2:4666632/1‑92 (MQ=255)

                      aTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGtg                                                                     >  1:5633982/1‑93 (MQ=255)

                       tttGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGtgc                                                                    <  2:2826966/93‑1 (MQ=255)

                       tttGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGtgc                                                                    <  2:4052501/93‑1 (MQ=255)

                       tttGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGtgc                                                                    <  1:1722214/93‑1 (MQ=255)

                       tttGTCTAGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGtgc                                                                    <  1:2575312/93‑1 (MQ=255)

                        ttGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGtgct                                                                   <  2:796354/93‑1 (MQ=255)

                         tGTCTGGCTCCACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGtgctt                                                                  <  1:2299182/93‑1 (MQ=255)

                          gTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGtgcttg                                                                 >  2:4480053/1‑93 (MQ=255)

                          gTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGtgcttg                                                                 >  1:1990182/1‑93 (MQ=255)

                           tCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGtgcttgc                                                                >  2:3809523/1‑93 (MQ=255)

                             tGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTgcttgctt                                                              <  1:1333266/93‑1 (MQ=255)

                             tGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTgcttgctt                                                              >  2:2568228/1‑93 (MQ=255)

                                   cacCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTg                                                        <  2:5763239/93‑1 (MQ=255)

                                      cTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGtt                                                     >  1:6050104/1‑93 (MQ=255)

                                       tGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGttt                                                    >  2:3875608/1‑93 (MQ=255)

                                       tGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGttt                                                    >  1:880452/1‑93 (MQ=255)

                                       tGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGttt                                                    >  2:2382617/1‑93 (MQ=255)

                                       tGCGCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGttt                                                    <  1:1763166/93‑1 (MQ=255)

                                        gcgcCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTg                                                   >  1:3291721/1‑93 (MQ=255)

                                          gcCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCt                                                 >  1:2621907/1‑93 (MQ=255)

                                           ccTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTc                                                >  1:4353202/1‑93 (MQ=255)

                                           ccTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTc                                                >  2:1988818/1‑93 (MQ=255)

                                            cTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCa                                               <  1:4709706/93‑1 (MQ=255)

                                             tGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCAt                                              >  1:415652/1‑93 (MQ=255)

                                             tGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCAt                                              >  2:2910209/1‑93 (MQ=255)

                                             tGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGGGGTTTGCTCAt                                              >  1:2297611/1‑93 (MQ=255)

                                                aGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGACGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTAATCTg                                           >  1:4087276/1‑93 (MQ=255)

                                                aGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTg                                           <  2:3440291/93‑1 (MQ=255)

                                                  ccGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTggg                                         <  1:5658254/93‑1 (MQ=255)

                                                      gccccccGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGcc                                     <  2:4862919/90‑1 (MQ=255)

                                                          cccGCCATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGAc                                 >  2:5992294/1‑93 (MQ=255)

                                                          cccGCCATGAACAAGCAGATGTACCGTGCCGCCGCCAGGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGAc                                 <  2:1704119/93‑1 (MQ=255)

                                                            cGCCATGAACCAGCAGATGGACCGTGCCGCCGCCACGCAGCATAATTTAGAGTTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAg                               >  1:74389/1‑93 (MQ=255)

                                                               cATGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTgg                            >  2:629036/1‑93 (MQ=255)

                                                                aTGAACCAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGATTGCTCATCTGGGGGCCAGACAGTGGc                           >  1:2891425/1‑93 (MQ=255)

                                                                     ccAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCa                      <  2:1820889/93‑1 (MQ=255)

                                                                     ccAGCAGATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCCGGGGGCCAGACAGTGGCAGTCa                      <  2:777979/93‑1 (MQ=255)

                                                                       agcagATTTCGCGTGCCGCCGCCACGTAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAgg                    <  1:427199/93‑1 (MQ=255)

                                                                       agcagATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAgg                    >  1:6133545/1‑93 (MQ=255)

                                                                       agcagATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAgg                    <  2:1317537/93‑1 (MQ=255)

                                                                       agcagATGTACCGCGCCGCCGCCACGCAGCATAATTTAGAGGCGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCAAGACAGTGGCAGTCAgg                    >  1:383434/1‑93 (MQ=255)

                                                                        gcagATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGc                   >  1:2962618/1‑93 (MQ=255)

                                                                        gcagATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGc                   >  2:3082695/1‑93 (MQ=255)

                                                                        gcagATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGc                   <  1:308687/93‑1 (MQ=255)

                                                                         cagATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCt                  >  1:970533/1‑93 (MQ=255)

                                                                         cagATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCt                  >  1:2130370/1‑93 (MQ=255)

                                                                          agATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCtt                 >  1:1525967/1‑93 (MQ=255)

                                                                          agATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCtt                 <  1:192055/93‑1 (MQ=255)

                                                                          agATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCtt                 >  1:602587/1‑93 (MQ=255)

                                                                           gATGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTtg                <  2:2665897/93‑1 (MQ=255)

                                                                            aTGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTtgt               <  1:101582/93‑1 (MQ=255)

                                                                             tGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTtgtg              <  2:4383050/93‑1 (MQ=255)

                                                                             tGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTtgtg              >  2:5461717/1‑93 (MQ=255)

                                                                             tGTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGGGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCCtgtg              >  1:4447596/1‑93 (MQ=255)

                                                                              gTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTgtgg             >  2:4256353/1‑93 (MQ=255)

                                                                              gTACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTgtgg             >  1:4979984/1‑93 (MQ=255)

                                                                               tACCGTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTgtggt            <  1:5100168/93‑1 (MQ=255)

                                                                                   gTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGata        <  1:617887/93‑1 (MQ=255)

                                                                                   gTGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGata        <  1:2157091/93‑1 (MQ=255)

                                                                                    tGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGatat       <  2:2102922/93‑1 (MQ=255)

                                                                                    tGCCGCCGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGatat       >  1:3917172/1‑93 (MQ=255)

                                                                                     gccgccgccACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATc      <  2:5034442/93‑1 (MQ=255)

                                                                                     gccgccgccACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATc      <  2:4171122/93‑1 (MQ=255)

                                                                                     gccgccgccACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATc      >  2:2796397/1‑93 (MQ=255)

                                                                                       cgccgcCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCgg    <  2:5505661/93‑1 (MQ=255)

GGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTC  >  NC_000913/3810663‑3810844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`