BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,131,917	A→G	N168S (AAC→AGC)	katG →	catalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,131,917	0	A	G	96.1%	846.1 / 14.9	257	N168S (AAC→AGC)	katG	catalase
Reads supporting (aligned to +/- strand): ref base A (7/3); new base G (126/121); total (133/124)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

Only 100 of 261 total aligned reads displayed.

GCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGG  >  NC_000913/4131827‑4132006

gCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCg                                                                                         >  2:3392224/1‑93 (MQ=255)

gCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCg                                                                                         >  1:205879/1‑93 (MQ=255)

 ccTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGt                                                                                        <  1:3843478/93‑1 (MQ=255)

  cTCGATAAAGCGCGTCTCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTg                                                                                       <  2:6076923/93‑1 (MQ=255)

  cTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTg                                                                                       >  2:1187020/1‑93 (MQ=255)

   tCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTgg                                                                                      >  2:3300291/1‑93 (MQ=255)

   tCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTgg                                                                                      >  1:1288785/1‑93 (MQ=255)

    cGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGgc                                                                                     <  1:3595774/93‑1 (MQ=255)

      aTAAAGCTCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGgcgc                                                                                   <  1:4444716/93‑1 (MQ=255)

      aTAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGgcgc                                                                                   <  1:4098828/93‑1 (MQ=255)

      aTAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGgcgc                                                                                   <  1:1218433/93‑1 (MQ=255)

       tAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCt                                                                                  >  1:1255726/1‑93 (MQ=255)

        aaaGCGCGTCGCCTGTTGTTGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTa                                                                                 <  2:4350451/93‑1 (MQ=255)

        aaaGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTa                                                                                 >  1:2311083/1‑93 (MQ=255)

         aaGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAg                                                                                >  2:5731726/1‑93 (MQ=255)

          aGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGa                                                                               >  2:2104545/1‑93 (MQ=255)

             gcgTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGaaaa                                                                            <  1:3042486/93‑1 (MQ=255)

             gcgTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGaaaa                                                                            <  1:3684635/93‑1 (MQ=255)

                tCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTc                                                                         <  2:4678797/93‑1 (MQ=255)

                tCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTc                                                                         >  1:5324519/1‑93 (MQ=255)

                gcGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTc                                                                         <  1:4524805/92‑1 (MQ=255)

                  gCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCg                                                                       <  1:526512/93‑1 (MQ=255)

                  gCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCg                                                                       >  2:150842/1‑93 (MQ=255)

                  gCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCg                                                                       >  1:2949362/1‑93 (MQ=255)

                  gCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCg                                                                       >  2:3594945/1‑93 (MQ=255)

                   ccTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCgg                                                                      >  2:5208635/1‑93 (MQ=255)

                     tgttgtGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCt                                                                    >  2:5304290/1‑93 (MQ=255)

                      gttgtGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCtt                                                                   >  1:6085733/1‑93 (MQ=255)

                      gttgtGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCtt                                                                   >  1:5931226/1‑93 (MQ=255)

                         gtgGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCg                                                                >  1:4995361/1‑93 (MQ=255)

                            gCCAATCAAACAGAAATATGGTCAAAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTAc                                                             <  2:3645448/93‑1 (MQ=255)

                               aaTCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCtt                                                          >  1:2580921/1‑93 (MQ=255)

                               aaTCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCtt                                                          <  1:97835/93‑1 (MQ=255)

                                ataaaaCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTc                                                         <  2:2184154/90‑1 (MQ=255)

                                aTCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTc                                                         >  2:1252724/1‑93 (MQ=255)

                                aTCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTc                                                         <  1:5561578/93‑1 (MQ=255)

                                 tCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCg                                                        <  2:1374785/93‑1 (MQ=255)

                                 tCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCg                                                        >  2:1130909/1‑93 (MQ=255)

                                    aaCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGtt                                                     >  2:5107978/1‑93 (MQ=255)

                                     aCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGttt                                                    <  1:2742198/93‑1 (MQ=255)

                                     aCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGttt                                                    >  2:2918154/1‑93 (MQ=255)

                                     aCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGttt                                                    <  2:2445305/93‑1 (MQ=255)

                                      cAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGtttt                                                   >  2:3343906/1‑93 (MQ=255)

                                      cAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGtttt                                                   >  1:1817552/1‑93 (MQ=255)

                                      cAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGtttt                                                   >  2:1537939/1‑93 (MQ=255)

                                       aGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTg                                                  >  2:2732103/1‑93 (MQ=255)

                                       aGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTg                                                  >  2:2685056/1‑93 (MQ=255)

                                        gAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTgg                                                 <  1:5237030/93‑1 (MQ=255)

                                        gAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTgg                                                 <  2:5172167/93‑1 (MQ=255)

                                        gAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTgg                                                 <  1:4331610/93‑1 (MQ=255)

                                           atatGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGc                                              >  1:4817838/1‑93 (MQ=255)

                                            tatGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGcc                                             <  2:2378736/93‑1 (MQ=255)

                                             atGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCg                                            >  2:1328826/1‑93 (MQ=255)

                                             atGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCg                                            <  1:5742824/93‑1 (MQ=255)

                                              tGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCgg                                           >  1:737371/1‑93 (MQ=255)

                                              tGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCgg                                           >  1:1935037/1‑93 (MQ=255)

                                                gTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTc                                         >  2:5364616/1‑93 (MQ=255)

                                                 tCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCg                                        <  1:5602037/93‑1 (MQ=255)

                                                   aGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTg                                      <  2:400955/93‑1 (MQ=255)

                                                    gAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGa                                     >  2:2552371/1‑93 (MQ=255)

                                                     aaaaTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGaa                                    <  1:3229994/93‑1 (MQ=255)

                                                       aaTCTCCTGGGGTGACCTGTTTATCCGCGCGGGTAGCGTGGGGCTAGAAACCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  <  1:81018/93‑1 (MQ=255)

                                                       aaTCTCCTGGGCCGCCGTGTTTATCCTCGCGTGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  <  1:4717725/93‑1 (MQ=255)

                                                       aaTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  >  2:5859522/1‑93 (MQ=255)

                                                       aaTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  >  1:5842693/1‑93 (MQ=255)

                                                       aaTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  <  1:4772216/93‑1 (MQ=255)

                                                       aaTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  >  1:2552879/1‑93 (MQ=255)

                                                        aTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGAc                                 <  2:1392507/93‑1 (MQ=255)

                                                        aTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGAc                                 <  1:172176/93‑1 (MQ=255)

                                                          ctcCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGt                               <  2:1990455/93‑1 (MQ=255)

                                                          ctcCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGt                               >  1:2132481/1‑93 (MQ=255)

                                                           tcCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTc                              <  1:1531470/93‑1 (MQ=255)

                                                           tcCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTc                              <  2:305273/93‑1 (MQ=255)

                                                           tcCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTc                              >  2:738180/1‑93 (MQ=255)

                                                            ccTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCt                             <  1:3277679/93‑1 (MQ=255)

                                                             cTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAGGACGTCTg                            >  2:3955474/1‑93 (MQ=255)

                                                             cTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTg                            >  1:1765728/1‑93 (MQ=255)

                                                              tGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTgg                           <  2:2625904/93‑1 (MQ=255)

                                                               gggCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTggg                          >  2:919875/1‑93 (MQ=255)

                                                                  ccGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAGCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAAc                       <  2:4875005/93‑1 (MQ=255)

                                                                    gACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCg                     <  1:1017449/93‑1 (MQ=255)

                                                                     aCCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCgg                    >  2:2299388/1‑93 (MQ=255)

                                                                     aCCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCgg                    >  2:4588091/1‑93 (MQ=255)

                                                                      ccTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGa                   <  2:1915981/93‑1 (MQ=255)

                                                                        tGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATc                 <  1:5070314/93‑1 (MQ=255)

                                                                        tGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATc                 >  2:5540690/1‑93 (MQ=255)

                                                                        cgTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATc                 <  1:5877000/92‑1 (MQ=255)

                                                                          tttATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTg               <  1:2028148/93‑1 (MQ=255)

                                                                          tttATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTg               <  2:5393285/93‑1 (MQ=255)

                                                                          tttATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTg               <  2:5939842/93‑1 (MQ=255)

                                                                             aTCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGAt            <  2:2215903/93‑1 (MQ=255)

                                                                             aTCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGAt            >  1:4092228/1‑93 (MQ=255)

                                                                              tCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATg           >  1:4971845/1‑93 (MQ=255)

                                                                                cTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGtt         >  2:2515535/1‑93 (MQ=255)

                                                                                cTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGtt         >  2:648581/1‑93 (MQ=255)

                                                                                  cgcgGGTAGCGTGGCGCTAGAAACCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTaa       <  1:5408706/93‑1 (MQ=255)

                                                                                   gcgGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTAGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGACGTGGATGGTaat      >  1:3309915/1‑92 (MQ=255)

                                                                                     gggTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTg    <  2:1775520/93‑1 (MQ=255)

                                                                                      ggTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTgg   >  2:1861639/1‑93 (MQ=255)

                                                                                       gTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTggg  >  1:1618235/1‑93 (MQ=255)

GCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGG  >  NC_000913/4131827‑4132006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`